• Genomics & Informatics
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• 제11권1호
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• pp.52-54
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• 2013

Proteins in DNAJ/K families are ubiquitous, from prokaryotes to eukaryotes, and function as molecular chaperones. For systematic phylogenomics of the DNAJ/K families, we developed the Eukaryotic DNAJ/K Database (EDD). A total of 12,908 DNAJs and 4,886 DNAKs were identified from 339 eukaryotic genomes in the EDD. Kingdom-wide comparison of DNAJ/K families provides new insights on the evolutionary relationship within these families. Empowered by 'class', 'cluster', and 'taxonomy' browsers and the 'favorite' function, the EDD provides a versatile platform for comparative genomic analyses of DNAJ/K families.

• BMB Reports
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• 제55권10호
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• pp.488-493
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• 2022

The specific pair of heat shock protein 70 (Hsp70) and Hsp40 constitutes an essential molecular chaperone system involved in numerous cellular processes, including the proper folding/refolding and transport of proteins. Hsp40 family members are characterized by the presence of a conserved J-domain (JD) that functions as a co-chaperone of Hsp70. Tumorous imaginal disc 1 (Tid1) is a tumor suppressor protein belonging to the DNAJA3 subfamily of Hsp40 and functions as a co-chaperone of the mitochondrial Hsp70, mortalin. In this work, we performed nuclear magnetic resonance spectroscopy to determine the solution structure of JD and its interaction with the glycine/phenylalanine-rich region (GF-motif) of human Tid1. Notably, Tid1-JD, whose conformation was consistent with that of the DNAJB1 JD, appeared to stably interact with its subsequent GF-motif region. Collectively with our sequence analysis, the present results demonstrate that the functional and regulatory mode of Tid1 resembles that of the DNAJB1 subfamily members rather than DNAJA1 or DNAJA2 subfamily proteins. Therefore, it is suggested that an allosteric interaction between mortalin and Tid1 is involved in the mitochondrial Hsp70/Hsp40 chaperone system.

• Genomics & Informatics
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• 제8권1호
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• pp.34-40
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• 2010

Radiofrequency (RF) radiation might induce the transcription of a certain set of genes as other physical stresses like ionizing radiation and UV. To observe transcriptional changes upon RF radiation, we exposed WI-38, human lung fibroblast cell to 1763 MHz of mobile phone RF radiation at 60 W/kg of specific absorption rate (SAR) for 24h with or without heat control. There were no significant changes in cell numbers and morphology after exposure to RF radiation. Using quantitative RT-PCR, we checked the expression of three heat shock protein (HSP) (HSPA1A, HSPA6 and HSP105) and seven stress-related genes (TNFRSF11B, FGF2, TGFB2, ITGA2, BRIP1, EXO1, and MCM10) in RF only and RF/HS groups of RF-exposed cells. The expressions of three heat shock proteins and seven stress-related genes were selectively changed only in RF/HS groups. Based on the expression of ten genes, we could classify thermal and non-thermal effect of RF-exposure, which genes can be used as biomarkers for RF radiation exposure.

• 한국동물생명공학회지
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• 제34권3호
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• pp.240-246
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• 2019

The aim of this study was to investigate effect of heat stress on expression levels of plasminogen activators (PAs) related mRNAs and proteins, and changes of PAs activity in porcine endometrial explants. The endometrial explants (200 ± 50 mg) were isolated from middle part of uterine horn at follicular phase (Day 19-21) and were pre-incubated in serum-free culture medium at 38.5℃ in 5% CO₂ for 18 h. Then, the tissues were transferred into fresh medium and were cultured at different temperature (38.5, 39.5, 40.5 or 41.5℃) for 24 h. The expression level of urokinase-type PA (uPA), type-1 PA inhibitor (PAI-1), type-2 PAI (PAI-2), and heat shock protein-90 (HSP-90) mRNA were analysis by reverse-transcription PCR and proteins were measured by western blotting. The supernatant were used for measurement of PAs activity. In results, mRNA and protein levels of HSP-90 was higher in 41.5℃ treatment groups than other treatment groups (p < 0.05). The expression of uPA, PAI-1, and PAI-2 mRNA were slightly increased by heat stress, however, there were no significant difference. Heat stress condition suppressed expression of active uPA and PAI-2 proteins (p < 0.05), whereas PAI-1 protein was increased (p < 0.01). Although PAI-1 protein was increased and active uPA was decreased, PAs activity was greatly enhanced by exposure of heat stress (p < 0.05). These results suggest that heat stress condition could change intrauterine microenvironment through regulation of PAs activity and other factors regarding with activation of PAs might be regulate by heat stress. Therefore, more studies regarding with regulatory mechanism of PAs activation are needed.

• 한국미생물·생명공학회지
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• 제25권2호
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• pp.129-136
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• 1997

Helicobacter pylori is a spiral-shaped, microaerophilic human gastric pathogen causing chronic-active gastritis in association with duodenal ulcer and gastric cancer. To investigate the possibility of H. pylori outer membrane proteins (OMPS) as the oral vaccine antigens, sarcosine-insoluble outer membrane fraction has been prepared from H. pylori NCTC 11637. The major OMPs having apparent molecular masses of 62 kDa, 54 kDa and 33 kDa were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which were identified as urease B subunit (UreB), heat shock protein (Hsp54 kDa) and urease A subunit (UreA), respectively. Minor protein bands of 57 kDa, 52 kDa, 40 kDa, 36 kDa and 31 kDa were also observed. The antigenicity of H. pylori OMPs and antigenic cross-reactivity among the strains were determined by immunoblot analysis using anti-H. pylori OMPs antisera or intestinal lavage solutions. The results showed that UreB, Hsp54 kDa, UreA and 40 kDa proteins vigorously stimulated mucosal immune response rather than systemic immunity. From this results, these proteins seemed to be useful as the antigen candidates for the oral vaccine. The immunoblotting results with surface proteins from eight isolated H. pylori strains were similar to that of H. pylori NCTC 11637. The IgA which had been arised from oral administration of H. pylori OMPs, was able to bind H. pylori whole-cells.

• Journal of Periodontal and Implant Science
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• 제40권2호
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• pp.61-68
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• 2010

Purpose: The present study was performed to clarify the relationship between periodontal disease severity and selected immunological parameters consisting of serum IgG titer against periodontopathogenic bacteria, the expression of the helper T-cell cytokine by gingival mononuclear cells, and patients' immunoreactivity to cross-reactive heat shock protein (HSP) epitope peptide from P. gingivalis HSP60. Methods: Twenty-five patients with moderate periodontitis had their gingival connective tissue harvested of gingival mononuclear cells during an open flap debridement procedure and peripheral blood was drawn by venipuncture to collect serum. The mean level of interproximal alveolar bone was calculated to be used as an index for periodontal disease severity for a given patient. Each of selected immunologic parameters was subject to statistical management to seek their correlations with the severity of periodontal disease. Results: A significant correlation could not be identified between serum IgG titers against specific bacteria (Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Streptococcus mutans) and the severity of periodontal disease. Expression of interleukin (IL)-10 by gingival mononuclear cells was statistically significant in the group of patients who had higher levels of alveolar bone height. However, a similar correlation could not be demonstrated in cases for IL-4 or interferon-$\gamma$. Patients' serum reactivity to cross-reactive epitope peptide showed a significant correlation with the amount of alveolar bone. Conclusions: It was concluded that expression of IL-10 by gingival mononuclear cells and patients' sero-reactivity to the cross-reactive HSP peptide of P. gingivalis HSP60 were significantly correlated with alveolar bone height.

• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4475-4482
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• 2014

Background: Cisplatin, a DNA damaging agent, induces apoptosis through increasing DNA fragmentation. However, identification of intrinsic resistance molecules against Cisplatin is vital to estimate the success of therapy. Bag-1 (Bcl-2-associated anthanogene) is one anti-apoptotic protein involved in drug resistance impacting on therapeutic efficiency. Elevated levels of this protein are related with increase cell proliferation rates, motility and also cancer development. For this reason, we aimed to understand the role of Bag-1 expression in Cisplatin-induced apoptosis in HeLa cervix cancer cells. Cisplatin decreased cell viability in time- and dose-dependent manner in wt and Bag-1L+HeLa cells. Although, $10{\mu}M$ Cisplatin treatment induced cell death within 24h by activating caspases in wt cells, Bag-1L stable transfection protected cells against Cisplatin treatment. To assess the potential protective role of Bag-1, we first checked the expression profile of interacting anti-apoptotic partners of Bag-1. We found that forced Bag-1L expression prevented Cisplatin-induced apoptosis through acting on Mcl-1 expression, which was reduced after Cisplatin treatment in wt HeLa cells. This mechanism was also supported by the regulation of heat shock protein (Hsp) family members, Hsp90 and Hsp40, which were involved in the regulation Bag-1 interactome including several anti-apoptotic Bcl-2 family members and c-Raf.

• 한국식품저장유통학회지
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• 제21권6호
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• pp.776-783
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• 2014

절단 배추를 예열처리하고 LDPE 필름 포장하여 단기저장하면서 이화학적인 품질특성과 갈변도를 조사하였다. $40^{\circ}C$에서 1, 2, 4, 8시간동안 예열처리한 후 SDS-PAGE 법으로 단백질 밴드를 조사한 결과, 예열처리구에서 대조구에 비하여 HSP 밴드로 추정되는 60, 39, 33, 12 kDa 밴드가 강하게 나타났으며, 이 중 4시간 예열처리구(HS 4)가 가장 발현율이 높았으며 중량감소율도 적었다. 8주 동안 저장 중 예열처리한 절단배추의 중량감소율 및 적정산도는 거의 변화가 없었으며, 가용성 고형분 함량은 4시간 예열처리구가 가장 적게 감소하였다. 갈변도는 HS 4가 가장 적었다(Con>HS 1>HS 8>HS 2>HS 4, p<0.05). PPO활성은 저장 중 모든 구에서 지속적으로 증가하였으며, HS 4가 가장 낮았고 총페놀성 화합물은 모든 처리구에서 유사하게 감소하였다. 예열처리한 절단배추에서 HSP 밴드의 발현을 확인하였으며, 4시간 예열처리구의 단기 저장 효과가 가장 좋았다.

• 한국환경농학회지
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• 제38권4호
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• pp.296-306
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• 2019

온도는 곤충의 발달, 성장, 생식에 중요한 요인이며, 또한 곤충의 생존에 직접적 관련있는 물리적 요인이다. 변온동물인 곤충은 생존을 위해 기후변화에 반응을 해야 하며, 저온과 같은 취약한 환경하에서도 다양한 생존전략을 발달시켜야 한다. 본 연구는 저온에 대한 적응에 기여하는 유전자를 동정하기 위해 배추좀나방 유충의 지방체를 저온과 상온에 노출시켜 전사체 분석을 수행하였다. 저온전사체에서는 chitinase, 표피단백질, Hsp23, chytochrome, Glutathione S transferase, phospholipase 2 유전자의 발현이 증가된 반면, 에너지 대사에 관여하는 UDP-당전이효소, trehalase, trehalose transporter는 오히려 발현이 감소하였다. 저온에 곤충이 노출되었을 때, 대사중심인 지방체의 유전자 발현의 변화가 곤충의 온도 적응을 이해하는 단서를 제공할 수 있을 것으로 기대된다.

• 한국가금학회지
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• 제40권1호
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• pp.31-40
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• 2013

본 연구는 브로일러에서 비타민 C와 E의 첨가 급여가 성장 능력 및 개체별 스트레스 경감 정도에 미치는 영향을 살펴보고자 하였다. 스트레스 반응 정도는 혈액과 각 조직별 세포들에 대한 텔로미어 함량, DNA 손상율 및 열손상단백질 유전자(HSP, HMGCR) 발현율을 분석하고 고찰하였다. 텔로미어 함량 및 감축율은 양적 형광접합보인법(Q-FISH)으로 분석하였고, DNA 손상율은 comet assay로 분석하였다. 열손상단백질 유전자 발현율은 HSP70, HSP90-${\alpha}$, HSP90-${\beta}$ 및 HMGCR을 표적으로 하여 real-time PCR로 분석하였다. 시험 결과, 급여 처리구 간에 체중, 증체량, 사료 섭취량, 사료 요구율 및 생존율 등 생산 능력의 차이는 없는 것으로 나타났다. 텔로미어 감축율에 있어서는 비타민 E 첨가 급여구가 대조구에 비해 유의하게 낮은 감축율을 보여 스트레스 경감의 효과를 나타내었다. DNA 손상율 또한 모든 비타민 첨가 급여구가 대조구에 비해 유의하게 낮은 양상을 보였다. HMGCR, HSP90-${\alpha}$ 및 HSP90-${\beta}$의 유전자 발현율에 있어서도 비타민 E 첨가 급여구가 대조구에 비해 유의하게 낮은 발현율을 나타내어 스트레스 경감 효과를 나타내었다. 이상의 결과에 따라 브로일러에 사료 내 비타민 E의 첨가 급여(100 mg/kg feed)는 성장 능력의 저하 없이 개체의 생리적 스트레스 정도를 경감시키는 바람직한 항산화 제재로 사료된다.