• BMB Reports
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• v.40 no.4
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• pp.554-561
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• 2007

Shen and colleagues (Lin et al., 2004) have recently shown that overexpression of the Drosophila DNA methyltransferase 2 isoform C, dDnmt2c, extended life span of fruit flies, probably due to increased expression of small heat shock proteins such as Hsp22 or Hsp26. Here, I demonstrate with immunoprecipitations that overexpressed dDnmt2c interacts with endogenous Hsp70 protein in vivo in S2 cells. However, its C-terminal half, dDnmt2c(178-345) forms approximately 10-fold more Hsp70-containing protein complexe than wild-type dDnmt2c. Overexpressed dDnmt2c(178-345) but not the full length dDnmt2c is able to increase endogenous mRNA levels of the small heat shock proteins, Hsp26 and Hsp22. I provide evidence that dDnmt2c(178-345) increases Hsp26 promoter activity via two heat shock elements, HSE6 and HSE7. Simultaneously overexpressed Hsp40 or a dominant negative form of heat shock factor abrogates the dDnmt2c(178-345)-dependent increase in Hsp26 transcription. The data support a model in which the activation of heat shock factor normally found as an inactive monomer bound to chaperones is linked to the overexpressed C-terminus of dDnmt2c. Despite the differences observed in flies and S2 cells, these findings provide a possible explanation for the extended lifespan in dDnmt2c-overexpressing flies with increased levels of small heat shock proteins.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2008.11a
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• pp.495-498
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• 2008

여러 가지 흡착제를 사용한 회분식 흡착성능 측정 실험에서 흡착성능은 음이온교환수지 > 입상활성탄 > Biomass > 분말활성탄 > 제올라이트 순으로 나타났으며, 모든 흡착제는 30분 안에 흡착평형 상태에 도달 하였다. 음이온교환수지를 이용한 회분식 흡착실험에서 최대흡착량은 pH 10에서 0.0483 mmol/g로 가장 높았고, 대체적으로 pH가 높은 범위에서 흡착이 잘 되는 것으로 밝혀졌다. 이것은 산화물 상태의 셀레늄이 pH 6 이하의 영역에서는 HSeO$_3^{-}$가 존재하며, PH 6$\sim$10 영역에서는 HSeO$_3^{-}$와 SeO$_3^{-2}$가 공존하며, pH 10 이상에서는 SeO$_3^{-2}$만 존재함을 알 수 있는데, 실험에서도 유사한 결과가 나온 것으로 사료된다. 입상활성탄을 이용한 흡착실험에서 최대흡착량은 pH 4.5에서 0.0574 mmol/g으로 가장 높았다. pH 4.5$\sim$6.5 범위에서는 대체적으로 비슷한 성능을 나타내었다. 입상활성탄의 표면전위 특성상 음이온으로 존재하는 셀레늄과 정전기적 반발력으로 인해 흡착이 거의 일어나지 않는 것으로 보이지만, 음이온 상태로 존재하는 셀레늄 이외의 금속간 화합물이나 물리적인 결합상태의 미세입자들이 흡착된 것으로 보인다.

• Pediatric Infection and Vaccine
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• v.3 no.2
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• pp.207-213
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• 1996

Herpes simplex virus(HSV) infections of the CNS are associated with significant morbidity and mortality even when appropriate antiviral therapy is administered. HSV infections of the brain can be subdivided into two categories : neonatal HSV infections, which usually are caused by HSV type 2, and herpes simplex encephalitis(HSE), which occur in patients over 3 months old and is nearly uniformly caused by HSV type 1. The clinical presentation of HSE is one of the focal encephalopathic process associated with altered levels of consciousness, fever, focal seizures and hemiparesis. But because of the lack of pathognomic clinical presentation and diagnostic procedure, the efforts to develop alternative diagnostic procedure have led to the use of new diagnostic technique such as polymerase chain reaction(PCR). We report a case of HSV type 1 encephalitis in 13 month old male infant who presented with altered level of consciousness, fever and focal seizures. With the use of the PCR, HSV-1 DNA was detected in cerebrospinal fluid from the patient. The symptoms and signs of encephalitis subsided by treatment with acyclovir in 14 days.

• Journal of the Korean Institute of Gas
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• v.13 no.1
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• pp.61-67
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• 2009

There are lots of energy facilities using gas(storage facility, compressed gas pipe, station, tank lorry) on the domestic. These major gas facilities cause major accidents associated with fire, explosion, toxic and etc. With the increased interest in reducing air pollution, supply of natural gas for gas vehicles is increasing. Thus, the number of establishments of LNG (Liquefied Natural Gas) and CNG(Compressed Natural Gas) stations is increasing as well. However, due to major gas accidents such as the fire and explosion accident of a Buchen LPG (Liquefied Petroleum Gas) station, it is difficult to establish a new station. In this research, we present quantitative risk assessment for LCNG;LNG multi-station and compare it result against individual risk criteria of HSE.

• Journal of Ginseng Research
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• v.31 no.1
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• pp.1-13
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• 2007

We found that the main bacterial metabolite M1 is an active component of orally administered protopanxadiol-type ginsenosides, and that the anti-metastatic effect by oral administration of ginsenosides may be primarily mediated through the inhibition of tumor invasion, migration and growth of tumor cells by their metabolite M1. Pharmacokinetic study after oral administration of ginsenoside Rb1 revealed that M1 was detected in serum for 24 h by HPLC analysis but Rb1 was not detected. M1, with anti-metastatic property, inhibited the proliferation of murine and human tumor cells in a time- and concentration-dependent manner in vitro, and also induced apoptotic cell death (the ladder fragmentation of the extracted DNA). The induction of apoptosis by M1 involved the up-regulation of the cyclin-dependent kinase(CDK) inhibitor $p27^{Kip1}$ as well as the down-regulation of a proto-oncogene product c-Myc and cyclin D1 in a time-dependent manner. Thus, M1 might cause the cell-cycle arrest (G1 phase arrest) in honor cells through the up/down-regulation of these cell-growth related molecules, and consequently induce apoptosis. The nucleosomal distribution of fluorescence-labeled M1 suggests that the modification of these molecules is induced by transcriptional regulation. Tumor-induced angiogenesis (neovascularization) is one of the most important events concerning tumor growth and metastasis. Neovascularization toward and into tumor is a crucial step for the delivery of nutrition and oxygen to tumors, and also functions as the metastatic pathway to distant organs. M1 inhibited the tube-like formation of hepatic sinusoidal endothelial (HSE) cells induced by the conditioned medium of colon 26-L5 cells in a concentration-dependent manner. However, M1 at the concentrations used in this study did not affect the growth of HSE cells in vitro.

• Journal of Energy Engineering
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• v.28 no.4
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• pp.29-34
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• 2019

In this study, a quantitative risk assessment was carried out for a hydrogen complex station. The complex fueling station to be evaluated was hydrogen-LPG, and the components of each station were analyzed and the risk was evaluated. The final risk is assessed by individual and societal risks, taking into account the impact of damage and the frequency of accidents. As a result of individual risk calculation for the hydrogen-LPG fueling station that is the subject of this study, the hydrogen-LPG type fueling station does not show the unacceptable hazardous area (> 1 × 10E-3) proposed by HSE. The level of individual risk for both the public and the worker is within acceptable limits. In societal risk assessment, the model to be interpreted shows the distribution of risks in an acceptable range(ALARP, As Low As Reasonably Practicable). To ensure improved safety, we recommend regular inspections and checks for high-risk hydrogen reservoirs, dispensers, tube trailer leaks, and LPG vapor recovery lines.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.50-50
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• 2003

We have identified and analysed a putative response regulator two-component gene (CaSKN7) from Candida albicans and its encoding protein (CaSkn7). CaSKN7 has an open reading frame of 1677bp. CaSKN7 encodes a 559 amino acid protein (CaSkn7) with an estimated molecular mass of 61.1 kDa. CaSKN7 is a homologue of a Saccharomyces cerevisiae SKN7 that is the regulator involved in the oxidative stress response. To study the role of CaSKN7, we constructed a CAI4-derived mutant strain carrying a homozygous deletion of the CaSKN7 gene. In the caskn7 disruptant cells, the formation of germ tube require shorter time than that in the congenic wild-type strain but the growth of mycelium delayed in liquid media. In contrast, the caskn7 disruptant cells attenuate the differentiation in solid media and the virulence in mouse model system. Expression level of hypha-specific and virulence genes - HYR1, ECE1, HWP1, and ALS1 - in the caskn7 disruptant cells increased as compared with that in the congenic wild-type strain in 10％ serum YPD. Skn7 in 5. cerevisiae was found to bind the HSE element from the SSA promoter, Also, CaSkn7 contains heat shock factor DNA-binding domain and the promoters of these genes have HSE-like sties. Therefore these results show that CaSKN7 regulate the differentiation and virulence of C. albicans.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.37 no.1
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• pp.1-8
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• 2024

This paper employs stochastic processing techniques to analyze explosion risks in plant facilities based on explosion return periods. Release probability is calculated using data from the Health and Safety Executive (HSE), along with annual leakage frequency per plant provided by DNV. Ignition probability, derived from various researchers' findings, is then considered to calculate the explosion return period based on the release quantity. The explosion risk is assessed by examining the volume, radius, and blast load of the vapor cloud, taking into account the calculated explosion return period. The reference distance for the design blast load model is determined by comparing and analyzing the vapor cloud radius according to the return period, historical vapor cloud explosion cases, and blast-resistant design guidelines. Utilizing the multi-energy method, the blast load range corresponding to the explosion return period is presented. The proposed return period serves as a standard for the design blast load model, established through a comparative analysis of vapor cloud explosion cases and blast-resistant design guidelines. The outcomes of this study contribute to the development of a performance-based blast-resistant design framework for plant facilities.

• Journal of the Ergonomics Society of Korea
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• v.31 no.1
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• pp.191-196
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• 2012

Objective: The Work-related musculoskeletal disorders(WMSDs) have been a problem on industrial occupational safety and health in Korean shipyard industry. It has been a big social problem. Background: As a result, Korean government enacted a law to prevent musculoskeletal disorders at work and some major companies have already implemented an ergonomics program. Method: This paper presents a study on the effect of an ergonomics program in a Korean shipbuilding company during the last ten years. Conclusion: The Incidence of WMSDs, the worker's compensation cost and the lost time were decreased after implementation of the ergonomics program. Application: The results of the publishing will help efficient operation of MSDs prevention activity in other companies.

• Journal of the Ergonomics Society of Korea
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• v.31 no.1
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• pp.197-202
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• 2012

Objective: The aim of this study is to introduce activities for job stress management in shipbuilding industry. Background: Recently, workers are exposed many job stress like workload, interpersonal conflict while management condition of company changes day by day. Particularly, shipbuilding industry has poor environment which aggravating the stress to the workers. So managing job stress may be more important. Method: The method of this study is to investigate preventive actions of job stress in "S" shipbuilding company. Results: This study through case of "S" shipbuilding company introduce activities of prevention of job stress including identification of level of job stress, managing of psychological clinic, training of worker and manager. Conclusion: In order to manage the job stress in the workplace, 1) The total management system based on the concern of the management in the workplace has to be constructed. 2) Computerized system for operation of total management system has to be constructed. 3) New employees need training for Job Stress. Application: Through this case study, I hope to help prevention activities of job stress in the workplace.