• 한국발생생물학회지:발생과생식
• /
• 제17권4호
• /
• pp.389-397
• /
• 2013

FGF9/16/20 signaling pathway specify the developmental fates of notochord, mesenchyme, and neural cells in ascidian embryos. Although a conserved Ras/MEK/Erk/Ets pathway is known to be involved in this signaling, the detailed mechanisms of regulation of FGF signaling pathway have remained largely elusive. In this study, we have isolated Hr-Erf, an ascidian orthologue of vertebrate Erf, to elucidate interactions of transcription factors involved in FGF signaling of the ascidian embryo. The Hr-Erf cDNA encompassed 3110 nucleotides including sequence encoded a predicted polypeptide of 760 amino acids. The polypeptide had the Ets DNA-binding domain in its N-terminal region. In adult animals, Hr-Erf mRNA was predominantly detected in muscle, and at lower levels in ganglion, gills, gonad, hepatopancreas, and stomach by quantitative real-time PCR (QPCR) method. During embryogenesis, Hr-Erf mRNA was detected from eggs to early developmental stage embryos, whereas the transcript levels were decreased after neurula stage. Similar to the QPCR results, maternal transcripts of Hr-Erf was detected in the fertilized eggs by whole-mount in situ hybridization. Maternal mRNA of Hr-Erf was gradually lost from the neurula stage. Zygotic expression of Hr-Erf started in most blastomeres at the 8-cell stage. At gastrula stage, Hr-Erf was specifically expressed in the precursor cells of brain and mesenchyme. When MEK inhibitor was treated, embryos resulted in loss of Hr-Erf expression in mesenchyme cells, and in excess of Hr-Erf in a-line neural cells. These results suggest that zygotic Hr-Erf products are involved in specification of mesenchyme and neural cells.

• Reproductive and Developmental Biology
• /
• 제31권2호
• /
• pp.77-82
• /
• 2007

This study was conducted to investigate the effects of oocyte maturational age and activation condition on in vitro development of porcine parthenogenetic embryos (parthenotes). Porcine follicular oocytes were matured in vitro for 30 to 44 hr. Maturation rate was examined during in vitro maturation (IVM) every 2 hr interval. The cdc2 kinase activity was measured at 36 and 44 hr of IVM. Some oocytes were activated at 36 or 44 hr of IVM by three different conditions; 1) single electric stimulation (1.5 kV/cm for $30{\mu}sec$; ES), 2) double electric stimulations (1.5 kV/cm for $30{\mu}sec$, followed by 1.0 kV/cm for $50{\mu}sec$ after 1 hr; ES+ES) or 3) ES+ES followed by culture in 6-dimethlyaminopurine (6-DMAP) for 4 hr (ES+ES+D), and cultured for 6-7 days. Maturation rate was significantly increased as culture period was increased to 36 hr (66.9%, p<0.05), and then gradually increased to 87.1% at 44 hr of IVM. The cdc2 kinase activity was decreased (p<0.05) with culture period prolonged from 36 hr to 44 hr. Lower blastocyst formation rate (4.3%, p<0.05) were obtained by ES in 36 hr-matured oocytes compared to other treatments (16.5 and 20.5%) in the same age and the same treatment in 44 hr-matured oocytes (15.0%). High blastocyst formation rate (23.6%) was obtained by ES+ES+D in 44 hr-matured oocytes (p<0.05). These results demonstrate that porcine oocyte activation and in vitro development of parthenotes can be affected by interactions between oocyte maturational age and activation condition.

• 한국습지학회지
• /
• 제11권1호
• /
• pp.91-97
• /
• 2009

본 연구는 강우강도에 따른 계분의 농지 환원시 비점유출로 인하여 수계에 미치는 영향을 연구하였다. 실험실 규모의 반응조를 제작하여 강우강도에 따른 표면유출수와 토양침투수에 대해 조사하였다. 표면유출수의 경우 10mm/hr 이하의 강우에서는 일어나지 않았으며, 20mm/hr에서 일부 유출되는 경향을 보였다. 32.4mm/hr시 가장 높은 표면유출을 보였으며 이때, $BOD_5$는 686mg/L, $COD_{Mn}$은 630mg/L의 농도를 나타내었다. 토양침투수의 경우도 32.4mm/hr의 강우시 많은 양의 계분퇴비가 유출되었고, 43.2mm/hr의 강우시에는 유출되는 양이 큰 폭으로 줄어들었다가 강우량이 증가할수록 유출되는 양이 다시 많아졌다. 비점유출량을 산정한 결과 $BOD_5$는 32.4mm/hr의 강우시 가장 높게 나타났고, T-N은 67.1mm/hr의 강우시 가장 높게 나타났다.

• Journal of Pharmaceutical Investigation
• /
• 제34권2호
• /
• pp.115-123
• /
• 2004

Geniposide and its related iridoid compounds have been used in traditional herbal medicine for thε treatment of Jaundice hepatic diseases and various inflammatorys. For the purpose to increase trandsdermal absorption, the hydrolyzed products of Gardeniae Furctus were identified and assayed of active ingredients and investigated trandsdermal absorption and anti-inflammatory effects. Geniposide was hydrolyzed to genipin by ${\beta}-glucosidase$ and it was suggested that genipin was more suitable form than geniposide for transdermal absorption by its lipophilic property. Using Franz type diffusion cell and the skin of hairless mouse, the permeation rate of hydrolyzed products and their emulsion preparation were determined. Genipin have more increased absorption ratio through the skin of hairless mouse than geniposide. Also, the emulsion of hydrolyzed products of extracts showed higher permeability than that of nonhydrolyzed preparations. After 9 hours $280.85\;{\mu}g/cm^2$ of genipin was absorbed and $193.52\;{\mu}g/cm^2$ in case of geniposide. The Js of geniposide and genipin were $26.27{\pm}4.11\;{\mu}g/cm^2/hr$ and $40.35{\pm}5.04\;{\mu}g/cm^2/hr$ respectively. After carrageenan injection, the swelling was increased repidly to 24 hr and maintained as plateau. but emulsion group weer reached about 2.5 mL and the swelling decreased successively form 24 hr to 72 hr. The anti-inflammation effects of extracts and hydrolyzed products emulsion were increased with significant difference with control group after 24 hr, 48 hr and 72 hr. In carrageenan induced edema, inhibition of swelling was increased in case of hydrolyzed product emulsion compare with nonhydrolyzed group at 24 hr, 48 hr and 72 hr after swelling. In histological study, the anti-inflammatory effects of hydrolyzed products were remarkable at 48 hr and 72 hr compare with nonhydrolyzed. Hydrolyzed products of Gardeniae Fructus extracts containing genipin would be a suitable preparation to increase the transdermal absorption and anti-inflammatory effects.

• Journal of Preventive Medicine and Public Health
• /
• 제51권6호
• /
• pp.298-309
• /
• 2018

Objectives: The objective of this study was to assess the relationship between systolic and diastolic blood pressure (SBP, DBP) and the risk of death from specific causes other than cardiovascular diseases. Methods: We calculated the risk of specific death by SBP and DBP categories for 506 508 health examinees in 2002-2003 using hazard ratios (HRs) and 95% confidence intervals (CIs) in a Cox proportional hazards model. Results: Compared to normal levels (SBP <120 or DBP <90 mmHg), stage I systolic and diastolic hypertension (SBP 140-159, DBP 85-89 mmHg, respectively) were associated with an increased risk of death from diabetes mellitus, alcoholic liver disease, and renal failure (HR, 1.83; 95% CI, 1.51 to 2.22; HR, 1.24; 95% CI, 1.06 to 1.46; HR, 2.30; 95% CI, 1.64 to 3.21; HR, 1.67; 95% CI, 1.27 to 2.20; HR, 1.99; 95% CI, 1.41 to 2.81; HR, 1.31; 95% CI, 0.99 to 1.73, respectively), but a decreased risk of death from intestinal pneumonia (HR, 0.64; 95% CI, 0.42 to 0.98; HR, 0.59; 95% CI, 0.39 to 0.91). Only stage II systolic hypertension (SBP ${\geq}160mmHg$) was associated with an increased risk of death from pneumonia, liver cirrhosis, and intestinal ischemia (HR, 1.54; 95% CI, 1.19 to 1.98; HR, 1.46; 95% CI, 1.00 to 2.15; HR, 3.77; 95% CI, 1.24 to 11.40, respectively), and stage I and II diastolic hypertension (SBP 140-159 and ${\geq}160mmHg$) were associated with an increased risk of death from intestinal ischemia (HR, 3.07; 95% CI, 1.27 to 7.38; HR, 4.39; 95% CI, 1.62 to 11.88, respectively). Conclusions: An increase in blood pressure levels may alter the risk of death from certain causes other than cardiovascular diseases, a well-known outcome of hypertension, although the mechanism of these associations is not well documented.

• 산업융합연구
• /
• 제20권7호
• /
• pp.31-36
• /
• 2022

경쟁우위 확보를 위해 인적자원을 효율적이고 효과적으로 활용하는 인사관리가 필요하다는 인식이 빠르게 확산하고 있다. 팬데믹으로 인해 노동시장과 근로환경이 빠르게 변화하는 상황에서 기업현장에서는 데이터와 같은 근거에 기반한 인사관리에 대한 관심이 확산하고 있다. 이에 본 연구에서는 기업현장에서 인사관리 직무를 수행하고 있는 전문가의 인식에 초점을 두고 데이터 기반 인사관리 실행과 활용 전도에 관해 확인하고자 한다. 연구대상은 경기도 판교에 소재한 IT 서비스 개발/제공 전문기업 3개사 인사기획/관리 직무전문가 3명으로 선정하였으며, 데이터 기반 인사관리 실행현황, 측정/분석 도구와 활용도 수준 파악 등에 집중하였다. 연구방법으로는 근거이론 기반의 개방코딩, 축코딩, 선택코딩 절차를 활용하였다. 심층면담을 통한 연구결과, 기업의 인사관리 측정지표는 사람, 생산성, 문화의 세 영역으로 나누어지며, 활용도가 높은 지표를 영역별로 구분할 수 있었다. 본 연구를 통해 연구대상 기업이 어떠한 측정도구와 메커니즘을 통해 인사관리 기능을 실행하고 있으며, 인사관리 실행 효과성과 효율성을 측정, 분석, 활용에 관한 기업현장의 인식을 확인할 수 있었다.

• 한국콘텐츠학회논문지
• /
• 제22권9호
• /
• pp.614-627
• /
• 2022

본 연구는 국내 HR Analytics 연구에서 활용한 데이터와 분석방법을 탐색하여 향후 연구를 위한 기초자료를 제공하고 HR Analytics 연구 현황을 밝히는 것을 목적으로 한다. 이를 위하여 체계적 문헌고찰 방법을 활용하여 국내 KCI 등재 학술지에 수록된 실증연구 논문 78편을 선정하였고 해당 논문을 근로자 생애주기에 따라 분류하여 검토하였다. 문헌고찰 결과 다음과 같은 결과를 얻을 수 있었다. 첫째, 근로자 생애주기에 따른 HR Analytics 연구 동향을 살펴본 결과, 선행연구에서는 구성원의 유지(retention)와 관련한 연구가 가장 많았고 성과 관리에 대한 연구가 그 뒤를 이었다. 둘째, HR Analytics 연구에서 사용한 데이터를 살펴본 결과 각 연구는 해당 연구문제에 따라 다양한 데이터(정형, 비정형)를 활용하고 있었으며 데이터 출처 또한 조직내부 시스템부터 국가 통계 DB까지 매우 다양한 것으로 확인하였다. 셋째, 문헌고찰 결과 국내 HR Analytics 연구는 기술적, 진단적 분석이 가장 많으며, 예측 및 처방과 관련한 연구는 미미한 수준임을 알 수 있었다.

• 아태비즈니스연구
• /
• 제13권1호
• /
• pp.125-139
• /
• 2022

Purpose - This study examines how employees' perception of HR department expertise affect their satisfaction of education and training. Moreover, this study explores that the HR department's communication activities moderate the main effects between satisfaction of education and training opportunities. Design/methodology/approach - This study predicts the positive relationship between employees' perceptions of HR department expertise and their satisfaction of education and training. Furthermore, the HR department's communication activities will strengthen this positive relationship. To test these hypotheses, this study used the Human Capital Corporate Panel (HCCP) datasets, especially individual-level 2017 data. The final number of samples is 1,947 for the analyses. In addition, this study utilized a hierarchical regression model with SPSS program. Finding - The results analyzed with the hierarchical regression model showed that the perceptions of HR department expertise had a positive relationship with satisfaction of provided educational and training. In addition, the HR department's communication activities moderated the relationship between perception of HR department expertise and satisfaction of education and training opportunities. Research implications or Originality - This study suggests academic and practical implications for future research in the human resource development filed by clarifying the critical factors to increase employees' satisfaction and transferability of education and training.

• 한국임상수의학회지
• /
• 제40권6호
• /
• pp.464-467
• /
• 2023

The autonomic regulation of heart rate (HR) is an indicator of the body's ability to adapt to an exercise stimulus. However, there are no reports on this topic in relation to Jeju horses used for racing. The aim of this study was to analyze the HR responses of Jeju horses during race trials and to investigate the correlation between HR and performance in these horses. HR data were collected from 17 healthy 2- to 7-year-old Jeju horses wearing HR monitors during race trials. Pearson correlation coefficients was used to determine the significance of the association between the race trial time and HR. A significant inverse correlation was observed between the race trial time (performance) and both the maximal HR (HRmax, r = -0.69, p < 0.01) and HR reserve (HRR) (HRmax - average HR during the warm-up period, r = -0.63, p < 0.01) and between the HRR and HRmax (r = 0.73, p < 0.01). Horses with a lower average HR during the warm-up period and higher heart rates during the trial achieved faster race trial times. This study reports the first HR measurements in Jeju horses during race trials, providing new insights into the HR response of this breed to exercise. The use of a noninvasive remote HR monitor system allowed assessment of exercise responses in the field, and the results suggest that both the HRR during warm-up period and HRmax may be useful performance indicators in Jeju horses.

• 한국통신학회논문지
• /
• 제30권8C호
• /
• pp.824-831
• /
• 2005

LR 영상을 이용하는 HR 영상 재구성에서 추정된 움직임 벡터의 오류는 재구성된 영상의 화질을 저하시킨다. 이를 해결하기 위해 움직임 추정과 HR 영상의 추정을 동시에 하는 방법들이 제안되었으나, 블록 정합법과 같은 간단한 방법을 사용하는 경우 추정된 움직임 벡터에는 여전히 오류가 발생한다. 본 논문에서는 정규화된 움직임 추정과 POCS를 이용한 HR 영상 재구성을 동시에 하는 IBR 재구성 방법을 제안한다. 이 방법에서는 어느 한 화소의 움직임 벡터가 이웃의 움직임 벡터들과 연관성이 있음을 이용하여 블록 정합법에 정규화를 적용하여 움직임 추정이 이루어진다. 제안된 방법을 사용한 실험 결과 종래의 방법에 비하여 움직임 추정이 개선되었으며 이에 따라서 재구성된 고해상도 영상의 화질과 PSNR이 현저히 개선되었다.