Yang, Kisuk;Lee, Jong Seung;Lee, Jaehong;Cheong, Eunji;Lee, Taeyoon;Im, Sung Gap;Cho, Seung-Woo
Proceedings of the Korean Institute of Surface Engineering Conference
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2016.11a
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pp.122.2-122.2
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2016
Surface topographical cues has been highlighted to control the fate of neural stem cells (NSCs). Herein we developed a hierarchically patterned substrate (HPS) platform for regulating NSC differentiation. The HPS induced cytoskeleton alignment and highly activated focal adhesion in hNSCs as indicated by enhanced expression of focal adhesion proteins such as focal adhesion kinase (FAK) and vinculin. hNSCs cultured on HPS exhibited enhanced neuronal differentiation compared to flat group. We also developed a graphene oxide (GO)-based hierarchically patterned substrates (GPS) that promote focal adhesion formation and neuronal differentiation of hNSCs. Enhanced focal adhesion and differentiation of hNSCs on the HPS was reversed by blocking the ${\beta}1$ integrin binding and mechanotransduction-associated signals including Rho-associated protein kinase (ROCK) and extracellular-regulated kinase (ERK) pathway, which may suggest a potential mechanism of beneficial effects of HPS. In addition, hNSCs on the HPS differentiated into functional neurons exhibiting sodium currents and action potentials as confirmed by whole cell patch-clamping analysis. The hierarchical topography can direct differentiation of NSCs towards functional neurons, and therefore would be an important element for the design of functional biomaterials for neural tissue regeneration applications.
Plant growth was greatly affected by the spectral distribution and light intensity of artificial lighting sources. In this study, the spectral characteristics of high power sodium (HPS) lamps and metal halide (MH) lamps produced by three different manufacturers were measured. Even though the spectral distribution of HPS lamps with lamp wattage of 250 W and 400 W was very similar, but the spectral light intensity by the manufacturers was different. Difference in the spectral light intensity of MH lamps by the manufacturers was increased with the increasing lamps wattage. Light intensity at the region of blue (B), green (G), red (R) and far-red (FR) light of HPS and MH lamps was also analyzed. HPS lamps showed the light intensity in order of R, FR, B and G light. The ratio of G, B, R and FR to photosynthetic photon flux (PPF) of HPS lamps with the lamp wattage of 250 W was 3.0-3.2%, 5.5-5.9%, 17.3-19.2% and 6.5-7.8%, respectively. For MH lamps, it showed the light intensity in order of R, FR, B, and G. The ratio of B, G, R, and FR to PPF of MH lamps with 250 W was 14.0-15.5%, 22.6-27.5%, 7.5-9.5% and 2.7-4.2%, respectively. HPS and MH lamps with 400 W had a relatively smaller ratio of R and FR to PPF than those with 250 W. HPS lamps showed that the ratio of light intensity of B and FR to R was 0.15-0.28 and 0.36-0.4, respectively. For MH lamps, the ratio of light intensity of B and FR to R was 1.26-2.72 and 0.27-0.56, respectively. From these results, it was concluded that the portion of blue light of MH lamps was higher than those of HPS lamps.
This study was performed to analyze the effect of light quality of discharge lamp on growth and phytochemicals contents of lettuce (Lactuca sativa L. cv. Jeokchima) grown under metal halide (MH) lamp, high-pressure sodium (HPS) lamp, and xenon (XE) lamp in a plant factory. Cool-white fluorescent (FL) lamp was used as the control. Photoperiod, air temperature, relative humidity, $CO_2$ concentration, and photosynthetic photon flux (PPF) in a plant factory were 16/8 h (day/night), $22/18^{\circ}C$, 70%, 400 ${\mu}mol{\cdot}mol^{-1}$, and 200 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. MH lamp had the greatest fraction of blue light (400-500 nm) of 23.0%. However, HPS lamp had the lowest fraction of 4.7% for blue light and the greatest fraction of 38.0% for red light (600-700 nm). At 11 and 21 days after transplanting, leaf length, leaf width, leaf area, shoot fresh weight, and shoot dry weight of lettuce as affected by the light quality of the discharge lamp were significantly different. The leaf area of lettuce grown under HPS, MH, and XE lamp increased by 45.7%, 16.3%, and 9.5%, respectively, as compared to the control. These results were similar for shoot fresh weight. Growth characteristics of lettuce grown under HPS lamp increased since HPS lamp had more fraction of red light. However, growth of lettuce grown under MH and XE lamp decreased since they had more fraction of blue light. As compared to the control, the ascorbic acid in lettuce leaves grown under discharge lamp decreased. The greatest anthocyanins accumulation of 0.70 mg/100 g was found at MH treatment. Anthocyanins content in lettuce leaves grown under XL and HPS lamp were 79.3% and 8.6%, respectively, compared with the control. Growth and phytochemicals contents of lettuce were highly affected by the different spectral distribution of the discharge lamp. These results indicate that the combination of discharge lamp or LED lamp for enhancing the light quality of discharge lamps is required to increase the growth and phytochemicals accumulation of lettuce in controlled environment such as plant factory.
Lee, Jong-Won;Kim, Ho Cheol;Jeong, Pyeong Hwa;Ku, Yang-Gyu;Bae, Jong Hyang
Horticultural Science & Technology
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v.32
no.3
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pp.346-352
/
2014
This research was carried out to investigate the effect of supplemental lighting on stable productivity of paprika (Capsicum annuum L.) during low radiation period of winter season. The supplemental lighting sources used in this research were high pressure sodium (HPS) and lighting emitting plasma (LEP). Photosynthetic photon flux density (PPFD) emitted from both lamps decreased as vertical distance from lamp increased. The PPFD of LEP lamps were twice more than that of the HPS lamp per unit distance, but the rate of decreased PPFD of t he LEP per unit distance was higher than that of HPS lamp. And different degrees of PPFD between HPS and LEP lamps by horizontal distance had a smaller degree of difference than by vertical distance at the 100 cm away point. As daily average PPFD measured at the top of the plant under the supplemental lighting during January, the supplemental lighting significantly increased radiation. Radiation of HPS and LEP lighting was 137% and 315% higher than control (without supplemental lighting = sunlight). Air temperature in the top of the plant was not significant different among treatments. HPS and LEP lighting had no effect on increase of flower settings. Leaf length and width with LEP lighting was the longest, photosynthetic was higher than those of other treatments. Supplemental lighting treatments significant increased fruit length and diameter. Especially LEP lighting treatment had a greater effect on fruit length and diameter. In conclusion, LEP lighting treatment during low radiation period greatly affected growth and production of paprika. Further research will be required for the suitable application of LEP lighting in paprika production.
In this experiment the effect of supplemental lighting on the growth and yield of cucumber (Cucumis sativus L. 'Fresh') plants during low radiation period of winter season were investigated in glasshouses using common high-pressure sodium (HPS) lamps and newly developed plasma lighting system (PLS) lamps. Plants grown without supplemental lighting were considered as a control. Supplemental lighting was provided from November 20th, 2015 to March 15th, 2016 to ensure 14-hour photoperiod (natural+supplemental light), also lamps were operated automatically when the outside sun radiation levels were less than $100W{\cdot}m^{-2}$. Spectral analysis showed that HPS lamp had a discrete spectrum, lacked of the radiation in the 400-550 nm wave band (blue-green light), but had a high output in the orange-red region (550-650 nm). A higher red light output resulted in an increased red to far-red (R/FR) ratio in HPS lamp. PLS had a continuous spectrum and had a peak radiation in green region (490-550 nm). HPS has 12.6% lower output in photosynthetically active radiation (PAR) but 12.6% higher output in near infra-red (NIR) spectral regions compared to PLS. Both HPS and PLS lamps emitted very low levels of ultra-violet radiation (300-400 nm). Supplemental lighting both from HPS and PLS lamps increased plant height, leaf number, internode number and dry weight of cucumber plants compared to control. Photosynthetic activity of cucumber plants grown under two supplemental lighting systems was comparable. Number of fruits per cucumber plant (fruit weight per plant) in control, PLS, and HPS plots were 21.2 (2.9 kg), 38.7 (5.5 kg), and 40.4 (5.6 kg), respectively, thereby increasing yield by 1.8-1.9 times in comparison with control. An analysis of the economic feasibility of supplemental lighting in cucumber cultivation showed that considering lamp installation and electricity costs the income from supplemental lighting increased by 37% and 62% for PLS and HPS lamps, respectively.
Journal of the Korea Institute of Information and Communication Engineering
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v.7
no.2
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pp.177-182
/
2003
In this study, we research the robust speech recognition for the syllables and phoneme units with the feature parameter including the voiced-unvoiced measures for the confusable words. In order to make it possible, we propose the measure representing the voiced-unvoiced degree by using the HPS(Harmonic Product Spectrum) information, used on pitch detection. We proposed this measures with the sharpnes, peak count and height measure of HPS. We reconstructed the feature parameter including this measures, then we performs the speech recognition experiments and compared with the typical feature parameters under the CVC type confusable syllables DB.
Co-existing pyloric submucosal masses with hypertrophic pyloric stenosis (HPS) are very rare and treating these lesions is always a problem. A 20-day-old boy presented with recurrent episodes of projectile non-bilious vomiting lasting for 5 days. HPS was suspected due to the presenting age and the symptoms. The sonography demonstrated not only circumferential wall thickening of the pylorus, but also a pyloric submucosal mass. At laparotomy, a 0.8 cm sized pyloric submucosal mass was identified along with a hypertrophied pylorus. Pyloric excision was performed due to the possibility of sustaining the symptoms and malignancy. The pathological report of the submucosal mass was ectopic pancreas. Coexisting pyloric lesions can be diagnosed along with HPS, and surgical excision, not just pyloromyotomy, should be considered in these circumstances. To the best of our knowledge, this is the first case report of pyloric ectopic pancreas and HPS to be diagnosed concurrently.
L. G. T. G. Rajapaksha;C. W. R. Gunasekara;P. S. de Alwis
Genomics & Informatics
/
v.20
no.4
/
pp.41.1-41.9
/
2022
The pathogen Gallibacterium anatis has caused heavy economic losses for commercial poultry farms around the world. However, despite its importance, the functions of its hypothetical proteins (HPs) have been poorly characterized. The present study analyzed the functions and structures of HPs obtained from Gallibacterium anatis (NCTC11413) using various bioinformatics tools. Initially, all the functions of HPs were predicted using the VICMpred tool, and the physicochemical properties of the identified virulence proteins were then analyzed using Expasy's ProtParam server. A virulence protein (WP_013745346.1) that can act as a potential drug target was further analyzed for its secondary structure, followed by homology modeling and three-dimensional (3D) structure determination using the Swiss-Model and Phyre2 servers. The quality assessment and validation of the 3D model were conducted using ERRAT, Verify3D, and PROCHECK programs. The functional and phylogenetic analysis was conducted using ProFunc, STRING, KEGG servers, and MEGA software. The bioinformatics analysis revealed 201 HPs related to cellular processes (n = 119), metabolism (n = 61), virulence (n = 11), and information/storage molecules (n = 10). Among the virulence proteins, three were detected as drug targets and six as vaccine targets. The characterized virulence protein WP_013745346.1 is proven to be stable, a drug target, and an enzyme related to the citrate cycle in the present pathogen. This enzyme was also found to facilitate other metabolic pathways, the biosynthesis of secondary metabolites, and the biosynthesis of amino acids.
Infantile hypertrophic pyloric stenosis (HPS) is a relatively common entity. A number of studies for the postoperative feeding schedule has been studied to allow for earlier hospital discharge and improve cost-effectiveness in the treatment of HPS. The purpose of this study was to compare 3 feeding-methods and to evaluate the usefulness of ad lib feeding for HPS. The authors retrospectively reviewed the records of 116 patients who underwent pyloromyotomy for HPS from 1995 to 2004. Three cases were excluded because of the duodenal perforation during pyloromyotomy. Three feeding-methods were defined as: Conventional feeding (>10 hours nothing by mouth and incremental feeding every 2 hours, C), Early feeding(for 4 to 8 hours nothing by mouth and incremental feeding every 2 hours, E), and Ad lib feeding (for 4 hours nothing by mouth and ad lib feeding, A). Time to normal feeing in C, E and A were $51{\pm}24$, $34{\pm}12$ and $24{\pm}6$ hours, respectively. Hospital-stay in C, E and A were $72{\pm}17$, $55{\pm}13$ and $43{\pm}12$ hours, respectively. There were statistically significant differences according to the method of feeding. Frequency of postoperative emesis in C, E and A were 38 %, 47 % and 53 %, but was not significant statistically. Ad lib feeding decreased time to normal feeding and hospital stay, and did not increase postoperative emesis. We conclude that ad lib feeding is recommended for patient with pyloromyotomy in HPS.
The purpose of this study was to identify the effect of human patient simulator(HPS)-based education on self-directed learning(SDL) and collective efficacy(CE) for nursing students. This study design was one group pre-posttest. The subjects were 2nd grade 92 students enrolling in the integrated practice. They have no previous experience of HPS-based education. HPS-based education included team based learning, skill training, taking a high-fidelity simulation with Medical Education Technologies, Inc (METI) simulator and being debriefed during 12 weeks. The pretest and posttest were conducted to understand the improvement in SDL and CE. After the subjects had participated in the HPS-based education, they showed statistically significant higher SDL(t=4.24, p=0.000) than before. However, there was no significant change in CE. Based on the results, this study suggests that SDL for nursing students were significantly improved by HPS-base education.
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