• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.141-141
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• 2017

The ubiquitin-proteasome pathway is the major regulatory mechanism in a number of cellular processes for selective degradation of proteins and involves three steps: (1) ATP dependent activation of ubiquitin by E1 enzyme, (2) transfer of activated ubiquitin to E2 and (3) transfer of ubiquitin to the protein to be degraded by E3 complex. F-box proteins are subunit of SCF complex and involved in specificity for a target substrate to be degraded. F-box proteins regulate many important biological processes such as embryogenesis, floral development, plant growth and development, biotic and abiotic stress, hormonal responses and senescence. However, little is known about the F-box genes in wheat. The draft genome sequence of wheat (IWGSC Reference Sequence v1.0 assembly) used to analysis a genome-wide survey of the F-box gene family in wheat. The Hidden Markov Model (HMM) profiles of F-box (PF00646), F-box-like (PF12937), F-box-like 2 (PF13013), FBA (PF04300), FBA_1 (PF07734), FBA_2 (PF07735), FBA_3 (PF08268) and FBD (PF08387) domains were downloaded from Pfam database were searched against IWGSC Reference Sequence v1.0 assembly. RNA-seq paired-end libraries from different stages of wheat, such as stages of seedling, tillering, booting, day after flowering (DAF) 1, DAF 10, DAF 20, and DAF 30 were conducted and sequenced by Illumina HiSeq2000 for expression analysis of F-box protein genes. Basic analysis including Hisat, HTseq, DEseq, gene ontology analysis and KEGG mapping were conducted for differentially expressed gene analysis and their annotation mappings of DEGs from various stages. About 950 F-box domain proteins identified by Pfam were mapped to wheat reference genome sequence by blastX (e-value < 0.05). Among them, more than 140 putative F-box protein genes were selected by fold changes cut-offs of > 2, significance p-value < 0.01, and FDR<0.01. Expression profiling of selected F-box protein genes were shown by heatmap analysis, and average linkage and squared Euclidean distance of putative 144 F-box protein genes by expression patterns were calculated for clustering analysis. This work may provide valuable and basic information for further investigation of protein degradation mechanism by ubiquitin proteasome system using F-box proteins during wheat development stages.

• The Journal of the Acoustical Society of Korea
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• v.35 no.1
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• pp.74-82
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• 2016

In this paper, a new automatic speech recognition (ASR) was proposed where ultrasonic doppler signals were used, instead of conventional speech signals. The proposed method has the advantages over the conventional speech/non-speech-based ASR including robustness against acoustic noises and user comfortability associated with usage of the non-contact sensor. In the method proposed herein, 40 kHz ultrasonic signal was radiated toward to the mouth and the reflected ultrasonic signals were then received. Frequency shift caused by the doppler effects was used to implement ASR. The proposed method employed multi-channel ultrasonic signals acquired from the various locations, which is different from the previous method where single channel ultrasonic signal was employed. The PCA(Principal Component Analysis) coefficients were used as the features of ASR in which hidden markov model (HMM) with left-right model was adopted. To verify the feasibility of the proposed ASR, the speech recognition experiment was carried out the 60 Korean isolated words obtained from the six speakers. Moreover, the experiment results showed that the overall word recognition rates were comparable with the conventional speech-based ASR methods and the performance of the proposed method was superior to the conventional signal channel ASR method. Especially, the average recognition rate of 90 % was maintained under the noise environments.

• The Journal of the Acoustical Society of Korea
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• v.18 no.6
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• pp.49-56
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• 1999

In many continuous speech recognition systems based on HMMs, decision tree-based state tying has been used for not only improving the robustness and accuracy of context dependent acoustic modeling but also synthesizing unseen models. To construct the phonetic decision tree, standard method performs one-level pruning using just single Gaussian triphone models. In this paper, two novel approaches, two-level decision tree and multi-mixture decision tree, are proposed to get better performance through more accurate acoustic modeling. Two-level decision tree performs two level pruning for the state tying and the mixture weight tying. Using the second level, the tied states can have different mixture weights based on the similarities in their phonetic contexts. In the second approach, phonetic decision tree continues to be updated with training sequence, mixture splitting and re-estimation. Multi-mixture Gaussian as well as single Gaussian models are used to construct the multi-mixture decision tree. Continuous speech recognition experiment using these approaches on BN-96 and WSJ5k data showed a reduction in word error rate comparing to the standard decision tree based system given similar number of tied states.

• 한국균학회소식:학술대회논문집
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• 2014.10a
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• pp.11-11
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• 2014

Fungal pathogens have huge impact on health and economic wellbeing of human by causing life-threatening mycoses in immune-compromised patients or by destroying crop plants. A key determinant of fungal pathogenesis is their ability to undergo developmental change in response to host or environmental factors. Genetic pathways that regulate such morphological transitions and adaptation are therefore extensively studied during the last few decades. Given that epigenetic as well as genetic components play pivotal roles in development of plants and mammals, contribution of microbial epigenetic counterparts to this morphogenetic process is intriguing yet nearly unappreciated question to date. To bridge this gap in our knowledge, we set out to investigate histone modifications among epigenetic mechanisms that possibly regulate fungal adaptation and processes involved in pathogenesis of a model plant pathogenic fungus, Magnaporthe oryzae. M. oryzae is a causal agent of rice blast disease, which destroys 10 to 30% of the rice crop annually. Since the rice is the staple food for more than half of human population, the disease is a major threat to global food security. In addition to the socioeconomic impact of the disease it causes, the fungus is genetically tractable and can undergo well-defined morphological transitions including asexual spore production and appressorium (a specialized infection structure) formation in vitro, making it a model to study fungal development and pathogenicity. For functional and comparative analysis of histone modifications, a web-based database (dbHiMo) was constructed to archive and analyze histone modifying enzymes from eukaryotic species whose genome sequences are available. Histone modifying enzymes were identified applying a search pipeline built upon profile hidden Markov model (HMM) to proteomes. The database incorporates 22,169 histone-modifying enzymes identified from 342 species including 214 fungal, 33 plants, and 77 metazoan species. The dbHiMo provides users with web-based personalized data browsing and analysis tools, supporting comparative and evolutionary genomics. Based on the database entries, functional analysis of genes encoding histone acetyltransferases and histone demethylases is under way. Here I provide examples of such analyses that show how histone acetylation and methylation is implicated in regulating important aspects of fungal pathogenesis. Current analysis of histone modifying enzymes will be followed by ChIP-Seq and RNA-seq experiments to pinpoint the genes that are controlled by particular histone modifications. We anticipate that our work will provide not only the significant advances in our understanding of epigenetic mechanisms operating in microbial eukaryotes but also basis to expand our perspective on regulation of development in fungal pathogens.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.42 no.4 s.304
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• pp.11-18
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• 2005

For expressing intention the human often use body languages as well as vocal languages. Of course the gestures using arms and hands are the representative ones among the body languages. Therefore it is very important to understand the human arm motion in human-computer interaction. In this respect we present here how to estimate 3D pose of human arms by using computer vision systems. For this we first focus on the idea that the human arm motion consists of mostly revolute joint motions, and then we present an algorithm for understanding 3D motion of a revolute joint using vision systems. Next we apply it to estimating 3D pose of human arms using vision systems. The fundamental idea for this algorithm extension is that we may apply the algorithm for a revolute joint to each of the revolute joints of hmm arms one after another. In designing the algorithms we focus on seeking closed-form solutions with high accuracy because we aim at applying them to human computer interaction for ubiquitous computing and virtual reality.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.10 no.2
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• pp.133-140
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• 2017

In this paper, we has proposed an automatic vehicle monitoring system based on computer vision technology. The real-time display system has displayed a system that can be performed in automatic monitoring and control while meeting the essential requirements of ITS. Another advantage has that for a powerful vehicle tracking, the main obstacle handing system, which has the shadow tracking of moving objects. In order to obtain all kinds of information from the tracked vehicle image, the vehicle must be clearly displayed on the surveillance screen. Over time, it's necessary to precisely control the vehicle, and a three-dimensional model-based approach has been also necessary. In general, each type of vehicle has represented by the skeleton of the object or wire frame model, and the trajectory of the vehicle can be measured with high precision in a 3D-based manner even if the system has not running in real time. In this paper, we has applied on segmentation method to vehicle, background, and shadow. The validity of the low level vehicle control tracker was also detected through speed tracking of the speeding car. In conclusion, we intended to improve the improved tracking method in the tracking control system and to develop the highway monitoring and control system.

• Journal of Ocean Engineering and Technology
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• v.34 no.5
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• pp.294-303
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• 2020

This study discusses data collection, calculation of wind and wave-induced resistance, and speed-power analysis of an 8,600 TEU container ship. Data acquisition system of the ship operator was improved to obtain the data necessary for the analysis, which was accomplished using SPA (Ship Performance Analysis, Park et al., 2019) in conformation with ISO15016:2015. From a previous operation profile of the container, the standard operating conditions of mean draft were 12.5 m and 13.6 m, which were defined with the mean stowage configuration of each condition. Model tests, including the load-variation test, were conducted to validate new ship performance and for the speed-power analysis. The major part of the added resistance of container ship is due to the wind. To check the reliability of wind-resistance calculation results, the resistance coefficients, added resistance, and speed-power analysis results using the Fujiwara regression formula (ISO15016:2015) and Computational fluid dynamics (Ryu et al., 2016; Jeon et al., 2017) analysis were compared. Wind speed and direction measured using an anemometer were used for wind-resistance calculation and the wave resistance was calculated using the wave-height and direction-data from weather information. Also, measured water temperature was used to calculate the increase in resistance owing to the deviation in water density. As a result, the SPA analysis using measured data and weather information was proved to be valid and able to identify the ship's resistance propulsion performance. Even with little difference in the air-resistance coefficient value, both methods provide sufficient accuracy for speed-power analysis. The differences were unnoticeable when the speed-power analysis results using each method were compared. Also, speed-power analysis results of the 8,600 TEU container ship in two draft conditions show acceptable trends when compared with the model test results and are also able to show power increase owing to hull fouling and aging. Thus, results of speed-power analysis of the existing 8,600 TEU container ship using the SPA program appropriately exhibit the characteristics of speed-power performance in deal conditions.

• The Journal of the Acoustical Society of Korea
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• v.40 no.5
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• pp.530-536
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• 2021

Recently, due to the development of deep learning, end-to-end speech recognition, which directly maps graphemes to speech signals, shows good performance. Especially, among the end-to-end models, conformer shows the best performance. However end-to-end models only focuses on the probability of which grapheme will appear at the time. The decoding process uses a greedy search or beam search. This decoding method is easily affected by the final probability output by the model. In addition, the end-to-end models cannot use external pronunciation and language information due to structual problem. Therefore, in this paper conformer with lexicon transducer is proposed. We compare phoneme-based model with lexicon transducer and grapheme-based model with beam search. Test set is consist of words that do not appear in training data. The grapheme-based conformer with beam search shows 3.8 % of CER. The phoneme-based conformer with lexicon transducer shows 3.4 % of CER.

• Journal of the Korean Society of Marine Environment & Safety
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• v.27 no.2
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• pp.266-274
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• 2021

Ship officers are subject to excessive job stress, which has negative physical and psychological impacts and may adversely affect the smooth supply and demand of human resources. In this study, a mobile web application was developed as a tool for systematic job stress measurement and management of officers and verified through quality evaluation. Requirement analysis was performed by ship officers and staff in charge of human resources of shipping companies, and the results were reflected in the application configuration step. The application was designed according to the waterfall model, which is a traditional software development method, and functions were implemented using JSP and Spring Framework. Performance evaluation on the user interface, confirmed that proper input and output results were implemented, and the respondent results and the database were configured in the administrator interface. The results of evaluation questionnaires for quality evaluation of the interface based on ISO/IEC 9126-2 metric were significant 4.60 for the user interface and 4.65 for the administrator interface in a 5-point scale. In the future, it is necessary to conduct follow-up research on the development of data analysis system through utilization of the collected big-data sets.

• Journal of Navigation and Port Research
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• v.46 no.4
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• pp.351-358
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• 2022

This study analyzed changes in the market concentration of Korean ocean-going shipping companies using shipping revenue based CR (Concentration Ratio), and HHI (Herfindahl - Hirschman Index) to examine the effects of the government's selection and concentration based the shipping reconstruction scheme. The results of this study showed that the market structure of the Korean shipping industry has changed from a competitive market to a rather concentrated market, as CR as well as HHI values have increased from 2019 to 2021. In particular, the market share of the deep-sea shipping lines has risen significantly compared to the intra-Asian short-sea shipping lines and the tramp carriers, implying that the Korean liner shipping market has become a monopoly, or highly concentrated oligopoly market. Compared to other shipping markets, the high rise in ocean freight rates (i.e. Asia-Europe America) was the leading cause of the increase in the revenues of the ocean-going shipping lines, and the increased fleet through preemptive government support has enabled them to achieve more revenues. As a result, it can be interpreted that the government's fleet expansion strategy has been more effective than expected, but it is too early to conclude if the market structure of the Korean ocean-going shipping companies has been strengthened.