• Journal of Advanced Marine Engineering and Technology
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• 제34권1호
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• pp.92-101
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• 2010

본 연구에서는 회전체를 지지하는 자기 베어링에 관하여, 2장에서는 적분형 서보계를 이용하여 횡축형 자기베어링 시스템(Horizontal-shaft Magnetic Bearing System: HMBS)에 대한 불확실성을 고려하면서 과도상태 응답개선에 대한 이론적인 고찰과 제어기설계 수식을 유도한다. 그리고 HMBS에 대해 물리 퍼래미터 변동에 대한 강인성과 외란의 영향을 저감하고 기준위치 변경에 따른 추종성을 갖도록 상태 피드백 제어기를 LMI 기법을 이용하여 설계한다. 3장에서는 설계한 제어칙을 가지고 시스템 불확실성의 변동에 대해 시간영역의 설계사양을 고려한 경우와 고려하지 않은 경우에 대하여 시뮬레이션을 행하고 실제 적용 가능성을 검토한다.

• 한국유체기계학회 논문집
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• 제19권3호
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• pp.5-9
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• 2016

The purpose of this paper is to define criteria to be used as part of the engineering design for an oil sands plant equipped with the steam assisted gravity drainage process. In this effort, the oil treatment process of an oil sands plant on a pilot scale was focused for detailed investigation. The thermodynamic properties of the process fluid, which is mainly composed of bitumen and water, were estimated with the CPA model. The commercial tool aspen HYSYS was used for the analysis throughout this work along with the provided input data and some necessary assumptions. From the simulation results, the heat and mass balances for a 300 BPD plant were established in order to define standard data for its modular design. In particular, the basis of design for equipment size, heat transfer areas, capital cost and operation cost was extensively discussed.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.180-185
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• 2005

Cytosine deaminase (cytosine aminohydrolase, EC 3.5.4.1) stoichiometrically catalyzes the hydrolytic deamination of cytosine and 5-fluorocytosine to uracil and 5-fluorouracil, respectively. Amino acid residues located in or near the active sites of the intracellular cytosine deaminase from chromobacterium violaceum YK 391 were identified by chemical modification studies. The enzymic activity was completely inhibited by chemical modifiers, such as 1mM NBS, chloramine-T, $\rho-CMB,\;\rho-HMB$ and iodine, and was strongly inhibited by 1mM PMSF and pyridoxal 5'-phosphate. This chemical deactivation of the enzymic activity was reversed by a high concentration of cytosine. Furthermore, the deactivation of the enzymic activity by $\rho-CMB$ was also reversed by 1mM cysteine-HCI, DTT and 2-mercaptoethanol. These results suggested that cysteine, tryptophan and methionine residues might be located in or near the active sites of the enzyme, while serine and lysine were indirectly involved in the enzymic activity. The intracellular cytosine deaminase from C violaceum YK 391 was assumed to be a thiol enzyme.

• 대한세포병리학회지
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• 제9권2호
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• pp.233-239
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• 1998

Clear ceil sarcoma(CCS) is an uncommon soft tissue sarcoma that occurs in tendons and aponeuroses, usually of the lower extremities and is believed to be of neural crest origin that have a capability to produce melanin. These tumors commonly metastasize and have a very poor prognosis. The fine needle aspiration cytologic finding of CCS is not well documented. We recently experienced a case of CCS. The patient was a 54-year-old male with painful swelling of the right inguinal area. Fine needle aspiration cytology revealed polygonal or fusiform tumor cells with clear or granular cytoplasm and vesicular nuclei containing one or two nucleoli. Immunohistochemical staining for S-100 protein and HMB-45 revealed strong positivity and variable developing stages of premelanosomes were observed by electron microscopy in the excised specimen.

• Journal of Microbiology and Biotechnology
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• 제25권6호
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• pp.880-886
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• 2015

Bacterial heme was produced from a genetic-engineered Escherichia coli via the porphyrin pathway and it was useful as an iron resource for animal feed. The amount of the E. coli-synthesized heme, however, was only few milligrams in a culture broth and it was not enough for industrial applications. To analyze heme biosynthetic pathways, an engineered E. coli artificially overexpressing ALA synthase (hemA from Rhodobacter sphaeroides) and pantothenate kinase (coaA gene from self geneome) was constructed as a bacterial heme-producing strain, and both the transcription levels of pathway genes and the intermediates concentrations were determined from batch and continuous cultures. Transcription levels of the pathway genes were not significantly changed among the tested conditions. Intracellular intermediate concentrations indicated that aminolevulinic acid (ALA) and coenzyme A (CoA) were enhanced by the hemA-coaA co-expression. Intracellular coproporphyrinogen I and protoporphyrin IX accumulation suggested that the bottleneck steps in the heme biosynthetic pathway could be the spontaneous conversion of HMB to coproporphyrinogen I and the limited conversion of protoporphyrin IX to heme, respectively. A strategy to increase the conversion of ALA to heme is discussed based on the results.

• Tuberculosis and Respiratory Diseases
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• 제48권5호
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• pp.788-793
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• 2000

결절성 경화증의 폐침윤은 드문 것으로 알려져 있는데 저자들은 결절성 경화증과 신장의 혈관근지방종으로 추적 관찰중인 환자에서 폐의 기흉이 발생하여 고해상 전산화단층촬영과 병리조직소견에서 HMB-45 양성인 평활근의 증식을 보인 폐의 림프관평활근종증을 진단받은 1예를 경험하였기에 보고하는 바이다.

• 대한수의학회지
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• 제35권2호
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• pp.375-381
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• 1995

Two intraocular tumors were identified in low and medium dosed groups of a carcinogenicity study using 200 males and 200 females Sprague-Dawley rats in Screening & Toxicology Research Center, Korea Research Institute of Chemical Technology. The tumors were grossly observed as white or yellow, unilateral nodules. They were approximately $1-2{\times}3-5mm$ in size. The tumors located in the region of iris and/or ciliary body invaded peripheral cornea. The microscopic features were usually composed of spindle cells arranged in parallel, forming gently curving bundles or whorls. The spindle cells had poorly defined cell boundaries, scant to moderate cytoplasm, fusiform nuclei and indistinct nucleoli. Mitotic figures were rare and areas of necrosis were present. The spindle cells had positive immunoreactivity for S-100 protein and vimentin but negative for desmin, collagen and HMB-45 antibody. In special histochemical studies, the spindle cells react with Gomori's stain for argyrophih fibers, Prussian blue stain for iron but negative with Masson-Fontana's stain for melanin granules. Ultrastructurally, cytoplasmic premelanosomes were not observed in the tumor cells due to the poor preservation of tumor masses. Based on the results, the tumors were diagnosed as amelanotic melanoma.

• Journal of Yeungnam Medical Science
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• 제16권1호
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• pp.108-113
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• 1999

저자들은 최근 58세 여자의 좌측 대퇴하부에 발생한 투명세포육종 1예를 경험하였기에 문헌고찰과 함께 보고한다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제44권1호
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• pp.29-33
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• 2018

Sinonasal mucosal melanoma (SNMM) in the maxillary sinus is a rare disease condition. Compared to oral mucosal melanoma, SNMM has a bulky, exophytic, and polypoid appearance, is weakly pigmented, and associated with unspecific symptoms. Due to these features, SNMM in the maxillary sinus has been misdiagnosed as nasal polyps and chronic sinusitis. In this case report, we described SNMM occurring in the right maxillary sinus simulated as a cystic or benign lesion. Cortical bone thinning and expansion were observed around the mass. The excised soft mass was encapsulated and weakly pigmented. The mass was clearly excised and covered with a pedicled buccal fat pad graft. Diagnosis using immunohistochemistry with S-100 and homatropine methylbromide-45 (HMB-45) is critical for proper treatment.

• 한국수의병리학회지
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• 제2권1호
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• pp.25-30
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• 1998

An in vivo model for human melanoma was established with the growth and metastasis of SK-MEL-2 cells. The tumor was introduced into C.B-17 SCID(severe combined immunodeficiency) mice intraperiotneally subcutaeously and intravenous inoculations. Tumors developed in 100% of mice inoculated subcutaneously and intraeritoneally both at site of inoculation and as metastatic tumor in the liver lungs and diaphragm. With intravenous inoculation 50% of mice showed metastasis in the spleen. Additionally metastatic foci that were not detected either by gross and/or standard histopathologic examination were demonstrated in the spleen and lungs by immunohistochemistry with HMB-45 monoclonal antibody. We conclude that the SCID mouse supports growth and metastasis of human malignant melanoma SK-MEL-2 cells.