• Journal of Ginseng Research
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• v.28 no.4
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• pp.173-182
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• 2004

We have shown that long-term intake of Korean red ginseng (KRG) delays disease progression in HIV-I infected patients. In the present study to investigate whether this slow progression was associated with protective human leukocyte antigen (HLA) alleles as well as with KRG-intake, we have performed clinical analysis of 31 HIV-1 infected patients who have been living for more than 10 years without any antiretroviral therapy. Average amount of KRG-intake over $130\;{\pm}16$ months was $4,797\;{\pm}4,921\;g$ and the annual decrease in CD4 T cell (AD) was $30\;{\pm}29{\mu}L$. We observed significant correlations among amount of KRG-intake, AD(r=-0.53, P < 0.01), and plasma HIV-1 RNA copy (r=-0.35, P < 0.05), along with a significant correlation between KRG-intake and HLA score AD(r=-0.49, P < 0.01), whereas there was no significant correlation between HLA score and AD or viral load. When the 31 patients were divided into 2 groups based on the amount of KRG-intake, the $AD(14/{\mu}L)$ in the 16 patients who had taken higher amounts of KRG was significantly less than that $(49/{\mu}L)$ in the 15 patients with a little or no KRG-intake (P < 0.01). These data indicate that KRG-intake sig­nificantly slows CD4 T cell depletion in HIV-1 infected patients.

• Proceedings of the Korean Information Science Society Conference
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• 2004.10a
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• pp.31-33
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• 2004

HLA (High Level Architecture) 는 모델링과 시뮬레이션 분야에서 상호 이질적인 서비스에 따른 어플리케이션의 상호운용성(interoperability)과 재사용성(reusability)을 증진시키기 위해 필요한 infrastructure를 개발하기 위한 청사진이다. RTI(RunTime Infrastructure)는 HLA 의 소프트웨어적 도구로써 IibTRI, FedExec, RTIExec의 세 가지 컴포넌트로 구성되어 있다. RTI는 분산환경에서 동적으로 다수대다수간의 통신을 제공하는 미들웨어 (middleware)이다. 대규모의 분산 시뮬레이션의 실행은 지리적으로 퍼져있는 많은 양의 컴퓨팅 자원을 필요로 할 수 있는데 이러한 환경이 가지는 보안과 동적 자원 할당이라는 문제점을 Globus Toolkit3(GT3)안의 Open Grid Service Arcitecture(OGSA)를 사용해서 해결할 수 있는 RTI-G를 개발해 왔고 이를 service-oriented RTI-G라 부른다. 이 논문에서는 service-oriented RTI on Grid 의 구조와 앞에 제시된 문제점들의 해결방안을 제시하고 실험한다.

• Proceedings of the Zoological Society Korea Conference
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• 1998.10b
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• pp.349.1-349
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• 1998

No Abstract, See Full Text

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.2
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• pp.128-135
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• 1984

Gamadi, a native rice cultivar from Nepal in which the panicle remains enclosed within its flag leaf sheath upto maturity, was crossed with different genetic marker testers of 12 linkage groups in order to analyze its linkage relationship. The results obtained from the experiment were summarized as follows: Normal segregations of all the genetic marker genes used in this experiment viz Cl, wx and Pla of linkage group I, Pn, Rd and Pub of linkage group III, and lg, g, Ps, gh, Hla, la, nl, bl, be and gl of linkage groups II, IV, V, VI, VII, VIII, IX, X, XI, and XII respectively confirmed the previous results, and also strongly indicated that the genetic constituent of the Gamadi and marker testers is same. 'Gamadiness' (the panicle enclosing character) was controlled by two complementary dominant genes with the segregation ratio of 9 Gamadi to 7 normal panicle-exserting types. These genes have been temporarily proposed as G-a and G-b for gamadiness. G-a gene was found to be linked with the neckleaf gene (nl) of linkage group Ⅸ with the crossover value of 0.3733$\pm$0.027. G-b gene appeared to be associated with the brittle culm gene (bc) of the linkage group XI with the crossover value of 0.2725$\pm$0.061.TEX>0.061.

• BMB Reports
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• v.43 no.3
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• pp.164-169
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• 2010

CpG-DNA, which contains unmethylated CpG dinucleotides in the context of specific sequences, has remarkable and diverse immunological effects, including induction of proinflammatory cytokine expression and regulation of the Th1/Th2 immune response. Here, we examined the immunostimulatory activities of double-stranded (ds) CpG-DNA in the human B cell line RPMI8226. To investigate whether dsCpG-DNA stimulates immune cells, we constructed a plasmid containing repeated dsCpG-DNA and produced dsCpG-DNA by PCR amplification and EcoR I digestion. PCR-amplified dsCpG-DNA alone did not have immmunostimulatory activity. However, dsCpGDNA encapsulated with lipofectin induced IL-8 promoter activation, HLA-DRA expression, and IL-8 expression in a CG sequence-independent manner. The effects of encapsulated dsCpGDNA were independent of minor endotoxin contamination. These findings suggest the potential use of dsCpG-DNA as a therapy for immune response regulation.

• Bulletin of the Korean Chemical Society
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• v.18 no.6
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• pp.623-628
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• 1997

A layered perovskite oxide, $RbLa_2Ti_2NbO_{10}$, was prepared and investigated for proton exchange and intercalation behaviors. Its protonated form, $Hla_2Ti_2NbO_{10}$, exhibits the Bronsted acidity and reacts with organic amines. Polyoxonuclear cation, 4Al_{13}$, was then introduced into the interlayer by refluxing octylamine-intercalated compound with an $Al_{13}$ pillaring solution. These layered oxides were characterized by X-ray diffractometer, thermogravimeter, FT-infrared spectrometer and elemental analyzer. It is observed that the polyoxonuclear cation-pillared material exhibits a bilayer structure and is thermally more stable than organic counterpart at higher temperatures. The surface area of the pillared material annealed at 400 ℃ was the value of 25.1 m²/g.

• Journal of the Korean Chemical Society
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• v.51 no.6
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• pp.536-542
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• 2007

Behcet's disease (BD) is a chronic inflammatory disorder, involving several organs. Inflammation in the disease is thought to be mediated by cytokines derived from T-helper type 1 (Th1) lymphocytes. Although the exact pathogenesis for BD is not completely understood, it has been suggested that the disease is triggered in genetically susceptible individuals by environmental factors, such as microbial agents. It is noted that multiple genes, including MHC (major histocompatibility complex) and non-MHC genes, are implicated in the pathogenesis of BD. This study tries to determine whether HLA-B51, IL-18, SLC11A1 and TNF-α polymorphisms are associated with susceptibility to Behcet's disease in Koreans. As a results, HLA-B51 was a genetic factor with the strongest association with BD. But it is still uncertain whether this HLA molecule is directly involved in the pathogenesis of BD. Although the IL-18 gene polymorphisms were not associated with a susceptibility to BD in the Korean population, the patients carrying the GG genotype at position 137 had a higher risk of developing the ocular lesions. This study suggests that the allele 3 and the genotype allele 3 / allele 3 of 5'-promoter (GT)n polymorphism in the SLC11A1 gene may have a protective effect for the development of BD in the Korean population. There were no evidences for genetic association conferred by the TNF-α gene with respect to susceptibility to BD.

• Journal of Periodontal and Implant Science
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• v.33 no.3
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• pp.331-340
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• 2003

본 연구의 목적은 인간의 동맥경화증에서 Porphyromonas gingivalis (P. gingivalis)와 인체 열충격단백 60의 T-세포 및 교차성 B-세포 epitope를 규명하고 수립된 T-세포주의 T-세포 주요조직적합체 양상을 파악하려는 데 있다. P. gingivalis 열충격단백-반응성 T 세포주와 환자의 혈청을 이용하여 P. gingivalis 열충격단백60 분자를 구성하는 104개의 중복성 합성 펩타이드의 T-세포 epitope과 B-세포 epitope을 규명하였다. 인체 열충격단백60에 대한 B-세포 epitope도 같은 방법으로 파악하였다. P. gingivalis, P. gingivalis 열충격단백60 또는 인체 열충격단백60에 대한 IgG 항체는 모든 동맥경화증 환자에서 상승하였다. P. gingivalis 열충격단백60의 3, 15, 24, 33, 45, 53, 64, 84, 88, 99번 펩타이드가 주요한 T-세포 epitope였고 이것들은 T-세포 및 B-세포 공동 epitope이기도 했다. 또한 인체 열충격단백60 교차반응 B-세포 epitope은 15, 29, 53, 56, 69, 74번 펩타이드로 판명되었다. 대부분 환자의 주요조직적합체는 $HLA-DRB1^{\ast}1504$와 $HLA-DZB1^{\ast}0603$으로 나타났다. 결론적으로 P. gingivalis 열충격단백60은 제 2급 주요조직적합제-제한적으로 분해되고 전달되었으며 이 단백질이 공통적인 T-세포 및 B-세포 epitope를 가지면서 동시에 인체 열충격단백60과 교차성 B-세포 epitope을 가지면서 동맥경화증의 면역조절기능에 관여한다고 볼 수 있다.

• Journal of the military operations research society of Korea
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• v.31 no.1
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• pp.42-57
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• 2005

Korea DM&S(Defence Modeling & Simulation) development plan includes construction of a distributed simulation model which enables us to construct, operate, integrate and connect war game simulations. US DoD and IEEE set the HLA/RTI as the standard of distributed simulation model. HLA/RTI models each simulator as a Federate and group of them as a Federation. Simulation with the model shows interoperability and finally executes a war game simulation. Because of different resolutions and features, it is not satisfied 'interoperability' and 'reusability' of simulation perfectly MRM (Multi Resolution Modeling) has been introduced, and many developed countries including the United States have studied the technology to simulate the model with different resolutions. This thesis proposes the need for introduction of MRM and analyzes both technical and operational validity when we apply MRM model to army war game simulations. For these purposes, we 1) find MRM main issues with the study about MRM basic concepts and case studies of MRM's applications, 2) present the need of MRM and its relation with DM&S development plan, and 3) analyze its technical and operational validity when we apply MRM to an example, CHANG-JO21 and JEON-TOO21.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.355-362
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• 2000

The Herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) gene in the pHLA-21 plasmid was inserted into a baculovirus (Hyphantria cunea nuclear polyhedrosis virus) expression vector (lacZ-HcNPV) to construct a recombinant virus gB-HcNPV expressing gB. Spodoptera frugiperda cells infected with this recombinant virus synthesized and processed gB of approximately 120 kDa, which cross-reacted with the monoclonal antibody to gB. The recombinant gB was identified on the membrane of the insect cells using an immunofluorescence assay. Antibodies to this recombinant raised in mice recognize the viral gB and neutralized the infectivity of the HSV-1 in vitro. These results show that the gB gene has the potential to be expressed in insect cells. They also demonstrate that it is possible to produce a mature protein by gene transfer in eukaryotic cells, and indicate the utility of the lacZ-HcNPV-insect cell system for producing and characterizing eukaryotic proteins. Furthermore, the neutralizing antibodies would appear to protect mice against HSV. Accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.