• The Journal of Korean Society of Virology
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• v.26 no.2
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• pp.259-267
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• 1996

In order to evaluate the effect of viral load on the prognosis of human immunodeficiency virus-1 (HIV-1)-infected individuals, immune complex dissociated (ICD) serum p24 antigen (p24) by acid treatment was retrospectively measured for 50 HIV-infected patients for 60 months. Among them, 27 patients were p24 positive (p24+) above 25pg/ml for $40.4{\pm}12$ months and 23 patients were negative (p24-). Follow-up periods from HIV diagnosis were $63.0{\pm}19$ months (range; 40-112) for the p24+ and $68.4{\pm}19$ months (range; 38-106) for the p24-, respectively (P>0.05)Mean CD4+T cell counts in the p24+ group decreased from $473{\pm}$277/ul (median;373) to $157{\pm}150/ul$ (median; 111) for $60{\pm}16$ months (5.3/month P280/ul (median; 476) to $432{\pm}285/ul$ (median;382) for $63{\pm}19$ months (2.5/month, P<0.01). From CD4+T cell count >200/ul, the patient who progressed to AIDS of <200/ul were 13 of 23 (56%) in the p24+ and 4 of 22 (18%) in p24-, respectively (p<0.01). And the number of death in two groups were 6 (22%) and 1 (4%), respectively (p<0.01). Presumed survival in two groups were about 12 and 24.5 years. These data suggest that viral load itself be very important for the prognosis of HIV-infected patients.

• BMB Reports
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• v.53 no.5
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• pp.248-253
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• 2020

Gene expression in HIV-1 is regulated by the promoters in 5' long-terminal repeat (LTR) element, which contain multiple DNA regulatory elements that serve as binding sites for cellular transcription factors. YY1 could repress HIV-1 gene expression and latent infection. Here, however, we observed that virus production can be increased by YY1 over-expression and decreased under YY1 depleted condition by siRNA treatment. To identify functional domain(s) of YY1 activation, we constructed a number of YY1 truncated mutants. Our data show that full-length YY1 enhances the viral transcription both through U3 and U3RU5 promoters. Moreover, the C-terminal region (296-414 residues) of YY1 is responsible for the transcriptional upregulation, which could be enhanced further in the presence of the viral Tat protein. The central domain of YY1 (155-295 residues) does not affect LTR activity but has a negative effect on HIV-1 gene expression. Taken together, our study shows that YY1 could act as a transcriptional activator in HIV-1 replication, at least in the early stages of infection.

• The Journal of Korean Society for School & Community Health Education
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• v.14 no.3
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• pp.77-87
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• 2013

Objective: The purpose of this study was to obtain the basic data for the establishment of the curriculum from the analysis of knowledge and attitudes about AIDS among dental hygiene students. Methods: Data was collected from the students of two colleges majoring in dental hygiene in Daegu, using questionnaire. They were the students of total 390. The analysis was made using t-test, (one-way ANOVA), and multiple linear regression analysis. These were conducted using SPSS 14.0 version. Results: The horizontal and vertical transmission in percent correct knowledge of HIV infection, most of the questions asked of respondents knew the correct answer, AIDS attitudes in the community education hours to receive AIDS education is important then the response was highest at 88.2 percent. AIDS knowledge is based on clinical practice, and AIDS attitudes in accordance with the educational experience of the average difference between groups investigated showed statistically significantly. HIV knowledge and attitudes, no oil, depending on the educational experience of the average difference between groups investigated showed statistically significantly. The grade, the higher knowledge of HIV was found to be lower in a statistically significant negative relationship showed (p<0.001), clinical experience, the more experience, knowledge of HIV was higher statistically significant positive showed a relationship (p<0.001), a higher attitude about HIV AIDS knowledge was higher showed a statistically significant positive relationship (p<0.001). Conclusion: Dental hygiene to prevent infection associated with AIDS in the curriculum and hands-on curriculum to enhance patient care through the AIDS patients in clinical management after graduating as a dental hygienist at the ethical responsibility is considered to be very strong.

• YAKHAK HOEJI
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• v.49 no.3
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• pp.217-224
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• 2005

Human Immunodeficiency Virus type 1 (HIV-l) based lentivirus vector has demonstrated great potential as gene therapy vectors mediating efficient gene delivery and long-term transgene expression in both dividing and nondividing cells. However, for clinical studies it must be confirmed that vector preparations are safe and not contaminated by replication competent lentivirus (RCL) related to the parental pathogenic virus, HIV-l. In this study, we would like to establish the method for titration and RCL detection of lentivirus vector. The titration was determined by vector expression containing the green fluorescent protein, GFP in transduced cells. The titer was $1{\times}10^7$ Transducing Unit/ml in the GFP expression assay and $8.9{\times}10^7$ molecules/ml in the real-time PCR. Also, for the detection of RCL, we have used a combination method of PCR and p24 antigen detection. First, PBS/psi and VSV-G region in the genomic DNA of transduced cells was detected by PCR assay. Second, transfer and expression of the HIV-1 gag gene was detected by p24 ELISA. In an attempt to amplify any RCL, the transduced cells were cultured for 3 weeks (amplification phase) and the supernatant of amplified transduced cell was used for the second transduction to determine whether a true RCL was present (indicator phase). Analysis of cells and supernatant at day 6 in indicator phase were negative for PBS/psi, VSV-G, and p24 antigen. These results suggest that they are not mobilized and therefore there are no RCL in amplification phase. Thus, real-time PCR is a reliable and sensitive method for titration and RCL detection of lentivirus vector.

• The Journal of Korean Society of Virology
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• v.28 no.1
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• pp.31-38
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• 1998

In Korea, all domestic made test systems for detecting antibodies in HIV-1 contain the antigens from human immunodeficiency type 1 (HIV-1) subtype B. However, because HIV-1 subtype O is significantly different in amino acid sequences from all other subtypes of HIV-1, there has been a need for developing a test for detecting antibodies in subtype O. For this purpose, the entire nucleotide sequence corresponding to the extracellular domain of the transmembrane glycoprotein of HIV-1 subtype O was synthesized with consideration of Escherichia coli condon usage. Various regions of the extracellular domain were cloned into E. coli expression vectors and tested for levels of protein production. The nucleotide sequence, named ECTM, that can encode a 129 amino acid-long peptide, was found to be expressed at a high level in E. coli. The protein of approximately 17 kDa specifically reacted with sera from individuals infected with HIV-1 subtype O. The ECTM protein was purified to near homogeneity by the CM-T gel chromatography, using concentrated, denatured inclusion bodies. In Western blot analysis, the purified viral antigen reacted with sera from individuals infected with subtype O more efficiently than subtype B. The enzyme linked immunoabsorbent assay (ELISA) system was developed using the subtype O viral protein and compared with the commercially available kit lacking the antigens from subtype O. The ELISA kit containing the subtype O antigen ECTM alone efficiently reacted with sera from individuals infected with subtype O. The subtype O antigen-containing kit produced a positive absorbence even when sera were diluted 512-fold, suggesting a high sensitivity. The commercially available kit also reacted with subtype O sera, but produced a negative result at a dilution of 8-fold. Our results suggest that the currently available kit may not be able to efficiently detect subtype O sera and that the viral protein developed in this study may be added to the current system to maximize the detection of sera from individuals infected with subtype O.

• Health Policy and Management
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• v.15 no.1
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• pp.57-77
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• 2005

The purpose of this study was to assess medical professionals' knowledge and attitude about AIDS. The subjects of study were medical doctors and dentists, belonging to Korea Association of Public Health Doctors. We sent a e-mailed questionnaire to 3,059 members and received 407 replies. Questionnaire was made on the basis of former studies' results and interviews with infected people. Major findings of this study were as follow. A lot of medical professionals had incorrect and biased knowledge about AIDS. More than half of them overestimated infection probability in case of being prickled with a contaminated needle. And many has negative attitude. The more they had correct informations about AIDS, the more they had possibilities to have friendly attitude to infected people. Incorrect knowledge cause excessive fears about AIDS, which amplify the stigma and discrimination. They contribute to people's vulnerability not only to HIV infection but also to other threats to health and well-being. It is in need of medical professionals' effort to increase their knowledge and improve attitude about AIDS.

• Tuberculosis and Respiratory Diseases
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• v.71 no.6
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• pp.395-399
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• 2011

Tuberculosis is one of the leading causes of mortality and morbidity worldwide. In 2010, there were 8.8 million (range, 8.5~9.2 million) incident cases of TB, 1.1 million deaths from TB among HIV-negative people and an additional 0.35 million deaths from HIV-associated TB. Due to such health-related importance, tuberculosis is one of areas being actively researched. Many numbers of tuberculosis-related papers are being presented each year, and in this review, it summarizes contents of a few papers that had been selected among the papers presented from 2009 to early 2011.

• Journal of Preventive Medicine and Public Health
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• v.57 no.3
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• pp.252-259
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• 2024

Objectives: This study investigated factors associated with the retention of people living with human immunodeficiency virus (HIV) on antiretroviral therapy (ART) during the first 3 years of treatment. Methods: A retrospective study using electronic health records was conducted at a tertiary hospital in Jakarta, Indonesia. Adult HIV-positive patients who started ART from 2010 until 2020 were included. A binary logistic regression model was used to identify factors associated with ART retention in the first 3 years. Results: In total, 535 respondents were included in the analysis. The ART retention rates for the first, second, and third years were 83.7%, 79.1%, and 77.2%, respectively. The multivariate analysis revealed a negative association between CD4 count when starting ART and retention. Patients with CD4 counts >200 cells/mL were 0.65 times less likely to have good retention than those with CD4 counts ≤200 cells/mL. The year of starting ART was also significantly associated with retention. Patients who started ART in 2010-2013 or 2014-2016 were less likely to have good retention than those who started ART in 2017-2020, with adjusted odds ratios of 0.52 and 0.40, respectively. Patients who received efavirenz-based therapy were 1.69 times more likely to have good retention than those who received nevirapine (95% confidence interval, 1.05 to 2.72). Conclusions: Our study revealed a decline in ART retention in the third year. The CD4 count, year of enrollment, and an efavirenz-based regimen were significantly associated with retention. Patient engagement has long been a priority in HIV programs, with interventions being implemented to address this issue.

• Korean Journal of Health Education and Promotion
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• v.11 no.2
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• pp.33-47
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• 1994

AIDS preventional efforts need to be guided by well trained health care personnel especially by school nurses for the adolescents. This study was designed to get basic data about AIDS educational program development for school nurses. The objectives of this study were to 1) identify AIDS knowledg of school nurses 2) identify AIDS attitudes of school nurses and 3) identify association between AIDS knowledge and sociodemographic characteristics. Data were collected from 173 subjects in Chonbuk province. Self-reporting questionnaire were administered during the period from 1st of June to 30th of June, 1994. AIDS related knowledge was measured by using 44 questions on cause(3 items), testing(3 items), mode of transmission(15 items), clinical manifestations(5 items), treatment(3 items), prevention(5 items), complication(4 items), infection control(3 items) and Using resources(3 items). AIDS related attitudes were measured by five point Likert scales using 13 questions on perceived threats from AIDS crisis (4 items), perceived severity to AIDS(2 items), perceived needs about psychosocial care for HIV infected patients(3 items) and perceived educational needs of AIDS(4 items). The collected data were analyzed by SPSS/PC/sup +/, using percentages, Mean and S.D. descriptive purpose and t-test or F for comparing the variables. The major findings were as follows: 1. Respondents ranged in knowledge of AIDS between 0 and 44 with the 33.79 mean score. Percentage of correctly answered respondents to each categories - mode of transmission : 87.0% - clinical manifestation : 85.0% - cause : 82.5% - prevention : 81.5% - treatment : 76.1% - infection control : 72.8% - testing of HIV infection 71.8% - using community resourses : 50.9% - complication : 45.8% 2. The respondents' attitudes of AIDS The majority(70%) showed higher perceived threat The majority(91.3%) showed higher perceived severity to AIDS crisis As a whole, perceived needs about psychosocial care for HIV infected patients negative The majority (over 96.5%) showed highly perceived educational needs of AIDS. 3. The relationship between AIDS knowledge and each of those general character. AIDS knowledge shows significant difference with age(F=3.50, p<.016), years of professional experience(F=4.14, p<.007) and received lecture about AIDS(F=4.54, p<.000). There was no significant difference between AIDS knowledge and job satisfaction.

• Tuberculosis and Respiratory Diseases
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• v.73 no.6
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• pp.336-341
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• 2012

Primary effusion lymphoma (PEL) is a rare type of lymphoma that arises in the body cavity without detectable masses. It is associated with human herpes virus-8 (HHV-8), Epstein-Barr virus (EBV), and human immunodeficiency virus (HIV). Recently, PEL unrelated to viral infection has been reported and it has been termed HHV-8 unrelated primary effusion lymphoma-like lymphoma (HHV-8 unrelated PEL-like lymphoma). Here, we report a case of HHV-8 unrelated PEL-like lymphoma in an 80-year-old woman. Chest X-ray and computed tomography revealed left-sided pleural effusion. Pleural effusion analysis and mediastinoscopic biopsy showed atypical cells that had originated from the B cells. The cells were positive for CD20 and bcl-2, but negative for CD3, CD5, CD21, CD30, CD138, epithelial membrane antigen, and HHV-8. Serological tests for HIV and EBV were negative. Considering the patient's age, further treatments were not performed. She has shown good prognosis without chemotherapy for more than 18 months.