• Microbiology and Biotechnology Letters
• /
• v.13 no.1
• /
• pp.19-25
• /
• 1985

The HIS5 gene of Saccharomyces cerevisiae host was encoded histidinol phosphate aminotransferase(E.C.: 2.6. 1.9). The HIS5 gene of Saccharomyces cerevisiae was cloned on plasmid pSH 530. This gene mighted be transcripted from a promoter of yeast gene both in E. coli and yeast hosts. We have determined the nucleotide sequence of the yeast HIS5 gene and its 5' and 3' flanking sequences. There are no large differences between the relative levels of HIS5 mRNA molecules with different 5' termini in represent and derepressed cell. In the DNA sequence upstream from the 5' termini of HIS5 mRNA we have found live closely related copies of a 9 base pair sequence. The sequence is also repeated in the 5' noncoding regions of HIS1, HIS3, HIS4, HIS5 and TRP5. Closely related sequence are not found flanking repeat sequence plays a role in the regulation of amino acid biosynthetic genes subject to the general amino acid control.

• Journal of the Korean Home Economics Association
• /
• v.18 no.1
• /
• pp.83-95
• /
• 1980

The purpose of this study is to investigate the reasons of Object Attachment Occurrence in infant and the meanings of it in his early development. For this purpose, the following hypotheses were supposed. 1. Infant's object Attachment will be a substitutional for mother attachment when he has opportunities to his mother during his first years. 2. Infant's Object Attachment will not impede the infant's normal development. In order to test this hypotheses 28 mothers whose infants show or have shown this phenomenon and 28 mothers whose infants show or have shown this phenomenon and 28 mothers whose infants do not show this one were interviewed about their child rearing practice and attitude. They were selected by the random sampling method from 310 household wives living at a delux Apt. in Seoul. The results obtained in this study are as follow ; 1. The less opportunities infant has for 1) his mother's breast feeding 2) having physical contact with his mother 3) spending time with his mother 4) receiving prompt response from his mother to his crying, the more he is apt to show this phenomenon. And infant whose first main attachment figure is not his mother also shows the same apt. 2. There was no difference between two groups in intelligence and personality. So it can be concluded that infant's Object Attachment is a substitutional phenomenon for mother attachment in the mother during his first years, and it does not impede infant's normal development.

• Lingua Humanitatis
• /
• v.1 no.1
• /
• pp.159-183
• /
• 2001

This paper surveys the meaning of the pen name ′Yi Sang(李箱)′ in the light of the arbitariness of language and the relationship among names, observing in three ways how the images of ′Sang′ (Box) appear differently in his works. In Particular, we look into (1) the relationship between a box and its inner boxes, (2) the divisional relationship in the box, and (3) the relationship between the box and an outer box. In Yi Sang′s work, image (1) gives shape to the relationship between the writer himself and his ancestors who are responsible for his present existence. Image (2) gives shape to two internally divided Yi Sangs, and is symbolized as a mirror that reflects two selves. Image (3) gives shape to a relationship between himself in the closed world and other people and/or a bigger outside world. All these symbolic relationships are fully represented in his work "Nalgae" (The Wing)". What is interesting is that Yi Sang has already employed these box images in his earlier poetry "Geonchuk-muhan-yukmyeon-gaucho" (Infinite Architectural Hexagon). furthermore, by visualizing his name as a sqaure($\square$), he means to suggest the intentional image of his pen name as a ′box′.

• Korean Institute of Interior Design Journal
• /
• v.19 no.1
• /
• pp.27-36
• /
• 2010

The purpose of this study is to define concept and characteristics of "Unphenomenon", the design concept conceived by Shiro Kuramata who is a world renowned Japanese interior designer and to explore how his ideas were materialized in his works. This study 1) examines his experience during his childhood reflected in his essay The scenery of the Unphenomenon' and training in Kuwazawa Design Institute that made Shiro Kuramata a true interior designer, and also considers how his design philosophy had been built up and realized under the unique social situation at the time. 2) defines the concept and characteristics of "Unphenomenon" as substance of his design philosophy by surveying remarks in his books and comments made by people around him in press interviews. 3) concludes his own design method and uniqueness by analyzing his works based on them. As a result, the study has founded that 1) transparency as recognitive realization of existence and non-existence: non-existence of physical property, connotation, ambiguity 2) expression of uncommonness by correcting and reinterpreting common prejudices: flotation, distortion of scale and shape 3) realization of insubstantiality by removing and replacing material and shape: replacement into light, removal of the structure, multiple layers of meaning.

• Weed & Turfgrass Science
• /
• v.5 no.4
• /
• pp.187-190
• /
• 2016

This study was conducted to determine phytotoxicity of domestic rice varieties and characteristic of sensitive gene involved in herbicide reaction of 4-HPPD (4-hydroxy phenylpyruvate dioxygenase) inhibiting herbicides. The five rice varieties were grown for 2 to 3 leaf stage on seedling trays and then transplanted into plastic pot: 4-Japonica type (Sangjubyeo, Sambaekbyeo, Sanduljinmi and Kumyoung) varieties and 1-Indica type variety (IR8). We trialled standard (14 g a.i. $10a^{-1}$) and double fold (28 g a.i. $10a^{-1}$) dose of benzobicyclon treatment at 10 days after transplanting in order to investigate phytoxicity. The Japonica-type Sangjubyeo showed no rice injury but Indica-type IR8 show 4-5 (standard) and 5-6 (double fold) phytoxicity levels. In spite of Japonica-type, Sanduljinmi and kumyoung showed 3-4 and 4-5 levels. Target resistant gene, Hydroxyphenylpyruvate dioxygenase inhibitor sensitive gene No.1 (HIS1) have been isolated from five domestic rice cultivars (Sangjubyeo, Sambaekbyeo, Sanduljinmi, Kumyoung and IR8). Results of the sequence through PCR, all five tested rice cultivars had HIS1 gene regardless to rice cultivars. And the difference between rice varieties from sequence of HIS1 were identified some variation in genes.

• The Journal of Korean Medical History
• /
• v.31 no.1
• /
• pp.1-12
• /
• 2018

Gyoojoon Lee is a prominent figure of Confucianism and traditional medicine representing southern region of Korea during late $19^{th}$ and early $20^{th}$ century, but studies on his achievements were just started merely a decade ago. We would provide foundation for the further multidisciplinary study on his legacy. We reviewed his chronology, written works, interactions with intellectual figures, and current studies on his works to provide foundations for more thorough research on his legacy. We found he has wide spectrum of studies in medicine, confucianism, literature, humanism and philosophy during the late Chosun dynasty, Korean empire and Japanese colonization period. He wrote twenty five books in diverse fields and was found to have interactions with fifty seven figures with idea during his lifetime which need more detailed examination. Multidisciplinary studies on Gyoojoon Lee is guaranteed for the future in depth study on his works including Buyunglon (theory of supporting Yang).

• Korean Institute of Interior Design Journal
• /
• v.13 no.5
• /
• pp.11-20
• /
• 2004

F. L. Wright was, from his early days, influenced by Japanese Culture and endeavored his original concept of orgonic orchitecture. In his 1st Golden Age, he devoted to establish an architectural concept of Organism which was proved by the theories of New Science and also had been universal in ancient Orient. Later, in his 2nd Golden Age, he tried to embody his unique concept in prairie houses and office buildings. The organic structure and spatial unit that actually applied to these works, were good examples of realization of the simplicity and continuity which he found out in Japanese culture. This paper is to study on the influence of Japanese culture on a course of Wright's embodying his organic architecture, and to study on a way of its realization in his works. To be concrete, main contents of the study are as follows; 1) the relationship between Wright's integrate perception and the orientalism 2) the influence of the traditional Japanese painting like woodblock prints and the aesthetic theory of the traditional Japanese pictorial art on Wright's design principles 3) the influence of Wright's experiences in Japan on his design principles 4) the influence of Wright's analysis of the traditional Japanese dwelling on his design principles 5) the course of Wright's embodying his organic architecture concept, and the way of its realization in his works.

• Genomics & Informatics
• /
• v.18 no.1
• /
• pp.11.1-11.3
• /
• 2020

In genome-wide association studies, pathway-based analysis has been widely performed to enhance interpretation of single-nucleotide polymorphism association results. We proposed a novel method of hierarchical structural component model (HisCoM) for pathway analysis of common variants (HisCoM for pathway analysis of common variants [HisCoM-PCA]) which was used to identify pathways associated with traits. HisCoM-PCA is based on principal component analysis (PCA) for dimensional reduction of single nucleotide polymorphisms in each gene, and the HisCoM for pathway analysis. In this study, we developed a HisCoM-PCA software for the hierarchical pathway analysis of common variants. HisCoM-PCA software has several features. Various principle component scores selection criteria in PCA step can be specified by users who want to summarize common variants at each gene-level by different threshold values. In addition, multiple public pathway databases and customized pathway information can be used to perform pathway analysis. We expect that HisCoM-PCA software will be useful for users to perform powerful pathway analysis.

• Korean Institute of Interior Design Journal
• /
• v.22 no.1
• /
• pp.97-104
• /
• 2013

This study aims at finding out the characteristics and influences of Libeskind's early drawing works through comparisons of each drawing. The importance of his experimental drawing works is not only their uniqueness but also relationship to creation and development of his architecture. Libeskind's musical and educational background had great impacts on forming his early drawings. A series of drawings including 'Micromegas', 'Chamberworks', and 'Theatrum Mundi' shows variety and experiments about reconstruction of three dimensional architectural fragments, abstractive line drawing and plane oriented painting. Libeskind himself cease to experiment drawing techniques but their characteristics still remain in his sketches, diagram and conceptual drawings. In spite of influences of undergraduate design studios, theoretical background of graduate studies, their experimental features are due to his continuous endeavor to make and develop his drawing skills and contents. As a result, his early drawings act as media and way to communicate and develop his concepts.

• Applied Biological Chemistry
• /
• v.28 no.1
• /
• pp.36-47
• /
• 1985

$HIS_5$ gene of Saccaromyces cerevisiae was cloned into pBR 322 and also into pSH 610 shuttle vector. $HIS_5$ gene was expressed as promoter of lactose operon(lacZ). And $HIS_5-lacZ$ fusion was intergrated into chromosome III of yeast. $HIS_5$ gene in yeast growth was controlled general amino acid control mechanism.