Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Studies on the $HIS_5$ Gene of Yeast -$HIS_5-lacZ$ fusion and regulation in Saccharomyces cerevisiae-

효모 $HIS_5$ 유전자에 관한 연구 -Saccharomyces cerevisiae의 $HIS_5-lacZ$ 융합과 조절-

  • Chung, Dong-Hyo (Department of Food Science and Technology, College of Industry Chung-ang University) ;
  • Oshima, Yasuji (Department of Fermentation Technology, Faculty of Engineering, Osaka University) ;
  • Nishiwaki, Kyoni (Department of Fermentation Technology, Faculty of Engineering, Osaka University)
  • 정동효 (중앙대학교 산업대학 식품가공학과) ;
  • 대도태치 (오사카대학 공학부 발효공학과) ;
  • 서협청이 (오사카대학 공학부 발효공학과)
  • Published : 1985.03.30
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Abstract

$HIS_5$ gene of Saccaromyces cerevisiae was cloned into pBR 322 and also into pSH 610 shuttle vector. $HIS_5$ gene was expressed as promoter of lactose operon(lacZ). And $HIS_5-lacZ$ fusion was intergrated into chromosome III of yeast. $HIS_5$ gene in yeast growth was controlled general amino acid control mechanism.

Saccharomyces cerevisiae의 HIS5 유전자는 세균 vector인 pBR 322와 shuttle vector pSH 610에 clone되었고 lactose operon의 promoter로서 발현되었다. HIS5-lac Z fusion은 효모의 제III번 염색체에 integration하였으며 HIS5 유전자는 general amino acid control을 받고 있었다.

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