• Title/Summary/Keyword: HEW

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Direct Purification of Lysozyme from Hen Egg White Using High Density Mixed Mode Adsorbent

  • KIM, WON KYUNG;BONG HYUN CHUNG
    • Journal of Microbiology and Biotechnology
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    • v.9 no.3
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    • pp.292-296
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    • 1999
  • The high density mixed mode adsorbent known by the trade name of Mimo-AD was used to purify lysozyme directly from the hen egg white (HEW). The homogenized hen egg white was treated with the adsorbent in a stirred vessel for lysozyme adsorption, and then the adsorbent, easily separated from the HEW by sedimentation, was packed into a column. The remaining HEW and contaminant proteins were removed by washing with pH 11 distilled water in an expanded-bed state, and subsequently the elution was performed with pH 12 distilled water in a packed-bed state. By this simple and rapid adsorption, washing, and elution procedure, lysozyme was purified to>95% with an overall recovery yield of 66%. This process offers a great potential for industrial application by allowing the extraction of lysozyme while retaining the commercial value of HEW.

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Modulation of Nrf2/ARE and Inflammatory Signaling Pathways by Hericium erinaceus Mycelia Extract

  • Jin, Kyong-Suk;Park, Ji-Young;Cho, Mi-Kyung;Jang, Ji-Hyun;Jeong, Jae-Han;Ok, Seon;Bak, Min-Ji;Song, Young-Sun;Kim, Myo-Jeong;Cho, Chung-Won;Jeong, Woo-Sik
    • Food Science and Biotechnology
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    • v.18 no.5
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    • pp.1204-1211
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    • 2009
  • Hericium erinaceus is an edible mushroom used as a medicinal food in Asian countries. In this study, the chemopreventive effects of H. erinaceus mycelia hot water extract (HEW) were evaluated. HEW remarkably induced the luciferase activity of the antioxidant response element (ARE), located in the promoter region of phase 2 and antioxidant genes and regulated by nuclear factor E2-related factor 2 (Nrf2). The up-regulation of ARE activity by HEW corresponded with the induction of Nrf2 and the antioxidant enzyme, hemeoxygenase-1. The inhibition of cyclooxygenase-2 (COX-2) activity is a promising effective approach in cancer chemoprevention, and HEW prominently suppressed COX-2 protein expression in HepG2 cells. Furthermore, HEW showed anti-inflammatory activity by modulating inflammatory mediators such as nitric oxide (NO), inducible NO synthase, tumor necrosis factor-${\alpha}$, interleukin-$1{\beta}$, and the transcription factor, nuclear factor-${\kappa}B$, in lipopolysaccharide-stimulated RAW 264.7 cells. These results suggest that H. erinaceus possessed anti-tumor and anti-inflammatory effects via the modulation of Nrf2/ARE and inflammatory signaling pathways, and may therefore have potential use as a natural chemopreventive agent.

Modelling of Web Based Remote Monitoring and Control System for Factory Automation (공장 자동화를 위한 웹 기반 원격 감시 및 제어시스템 모델링)

  • Yoon, Jong-Joon;Choi, Jin-Suk;Lee, Jeong-Bae;Gang, Sun-Ju;Im, In-Taek;Kim, Sam-Ryong;Lee, Yeong-Ran
    • Proceedings of the Korea Information Processing Society Conference
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    • 2002.11c
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    • pp.2475-2478
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    • 2002
  • 본 논문은 공장 자동화에 포괄되는 산업용 원격 제어 시스템을 웹을 통해 구성하고 이를 모형화하여 시스템이 동작되어지는 과정을 직접 구현 및 실험하는데 그 목적을 두고 있다. 이를 위해서 Lego Robotics Invention System과 Real-Time Linux를 이용하여 신발 산업의 공정의 모형을 제작하며 웹에서 이를 제어 및 감시하기 위한 각 모듈들은 JAVA로 제작하여 산업 현장에서의 원격감시 및 제어 시스템의 적용 방법을 제시하고자 한다.

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IEEE 802.11ax 차세대 무선랜 표준화 동향

  • Son, Ju-Hyeong;An, U-Jin;Go, Geon-Jung;Gwak, Jin-Sam
    • Information and Communications Magazine
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    • v.33 no.10
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    • pp.3-9
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    • 2016
  • AP와 단말이 혼재된 무선랜 환경에서 사용자가 실질적으로 성능 향상을 체감할 수 있는 고효율의 무선랜 표준 개발을 목표로 14년 5월 IEEE 802.11 HEW (High Efficiency WLAN) Task Group이 결성되어 본격적인 표준화 논의가 진행되고 있다. 기존의 무선랜 표준들이 최대전송률 향상에만 초점을 맞췄던 것에 비해, 11ax 표준은 고밀도 중첩 무선랜 환경에서 사용자별 실질적 체감 성능 향상을 목표로 하고 있다. 본 고에서는 6세대 무선랜 표준이 될 IEEE 802.11ax HEW 표준의 표준화 동향 및 주요 요소 기술들에 대해 살펴본다.

비교이론에 근거한 은유해석 방법

  • 조혜경
    • Proceedings of the Korean Society for Language and Information Conference
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    • 2001.06a
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    • pp.283-303
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    • 2001
  • This paper is to identify hew to interpret metaphorical expressions on the basis of the Comparison Theory. The metaphor is widely distributed throughout various aspects of language. There are three important conventional theories that are related to the interpretation of metaphor: substitution, interaction and comparison theory. This paper attempts to interpret several metaphors representative of the seven paths. we can conclude that the seven paths of metaphor interpretation can be applied to the interpretation of various types metaphor expressions with more precision and they can convey the meaning of a metaphor according to the speaker’s intention.

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Analysis of dual integral equation formulated for EM waves scattered by wedges (쉐기형 산란체에 의해 산란된 전자파에 대한 쌍적분 방정식 해석)

  • Kim, Se-Yun;Na, Jeong-Ung;Sin, Sang-Yeong
    • Proceedings of the KIEE Conference
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    • 1985.07a
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    • pp.344-347
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    • 1985
  • Hew dual integral equation for electromagnetic field scattered by an arbitrary dielectric wedge is formulated. In order to check the validity and physical meaning of the formulated equation, it is applied to the well-known case which is the diffraction by a perfectly conducting wedge.

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Effect of Immune Serum on the survival and Respiration of Brucella (Brucella 면역혈청이 균생존과 호흡에 미치는 영향)

  • Ahn, Tai-Hew
    • The Journal of the Korean Society for Microbiology
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    • v.5 no.1
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    • pp.33-39
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    • 1970
  • 정상혈청과 면역혈청의 존재하에 있어서 균의 생존율이 호흡율과 어떠한 관계가 있는가 Brucella 균을 사용하여 실험해보았다. 혈청의 원액에 균을 $37^{\circ}C$ 3시간 방치했다가 흔히 쓰는 평판주입법에 의하여 생존균수를 측정했고, 균호흡은 Warburg 장치를 사용하여 측정했다. 그 결과를 요약하면 다음과 같다. 혈청의 살균작용과 균호흡억제작용 간에는 극히 대조적인 현상을 엿볼 수 있었는데, 그것은 균호흡이 억제되면 될 수록 생존균수는 그만큼 증가되었다는 사실이다. Brucella 면역혈청의 살균계수가 $1.4{\sim}1.6$이었는데 반해 정상혈청은 2.7나 되었으며, 그 차는 무려 $log_{10}$ 일단위 이상이나 되었다. 그러나 면역혈청내에서의 균호흡율은 정상혈청중에서의 호흡율의 반도 안되었다. 면역체가 보체와 더불어 이러한 호흡억제 작용을 나타낸 것 같으며 이렇게 호흡이 억제된 상태에 있는 균은 혈청의 살균인자에 보다 무감각한 것 같다. 즉 in vitro에서 Brucella 면역체는 어느 일정량 이상 존재하면 균호흡을 억제하고 동시에 혈청의 살균인자로부터 균을 보호해 준 역할을 한다고 볼 수 있다.

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Switching Functions Model of a Three-phase Voltage Source Converter (VSC)

  • Fazeli, Seyed Mahdi;Ping, Hew Wooi;Abd Rahim, Nasrudin Bin;Ooi, Boon Teck
    • Journal of Power Electronics
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    • v.17 no.2
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    • pp.422-431
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    • 2017
  • The equivalent circuit of a three-leg, four-wire voltage source converter (VSC) is derived using switching functions. Simulations and experiments are conducted (i) to investigate the effects of the zero sequence on VSCs when a three-phase imbalance exists and (ii) to use the consistency of simulations and laboratory experiments to validate the equivalent circuit. The impact of a three-phase imbalance on the VSC has yet to be fully investigated because of the lack of an equivalent circuit to show rigorously how the zero sequence currents flow through the VSC.

Dynamic Behavior of Rotating Cantilever Pipe Conveying Fluid with Moving mass (이동질량을 가진 유체유동 회전 외팔 파이프의 동특성)

  • Son, In-Soo;Yoon, Han-Ik
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2005.05a
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    • pp.308-311
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    • 2005
  • In this paper, we studied about the effects of the rotating cantilever pipe conveying fluid with a moving mass. The influences of a rotating angular velocity, the velocity of fluid flow and moving mass on the dynamic behavior of a cantilever pipe have been studied by the numerical method. The equation of motion is derived by using the Lagrange's equation. The cantilever pipe is modeled by the Euler-Bemoulli hew theory. When the velocity of a moving mass is constant, the lateral tip-displacement of a cantilever pipe is proportional to the moving mass and the angular velocity. In the steady state, the lateral tip-displacement of a cantilever pipe is more sensitive to the velocity of fluid than the angular velocity, and the axial deflection of a cantilever, pipe is more sensitive to the effect of a angular velocity.

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