• 한국식품과학회지
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• 제29권2호
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• pp.348-354
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• 1997

본 연구는 여러가지 유해세균과 인체장암세포에 대한 다양한 농도에서의 마늘 추출물의 효과를 고양하고자 계획하였다. 미생물에 대한 항균효과는 그 최소 발육저지농도가 alliin의 경우에는 $5,000{\sim}20,000\;ppm$, 그리고 ethanol extract의 경우에는 $1,250{\sim}10,000\;ppm$이었으며, 또한 이들 성분의 암세포증식억제는 첨가농도가 증가함에 따라 점차 강하게 억제시킴을 알 수 있었다. 그리고 HCT-15의 형태적 관찰에서도 위축됨을 알 수 있었으며, 위의 결과들로 부터 항균과 암세포증식억제에 있어서의 활성물질은 열에 안정하고 에탄올에 의해 추출되는 성분임을 알 수 있었다.

• 생약학회지
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• 제39권4호
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• pp.347-351
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• 2008

The methanol (MeOH) extract of the rhizome of Alpinia officinarum Hance (Zingiberaceae) demonstrated a potent inhibition on the proliferation of cultured human tumor cell lines such as MES-SA (human uterine carcinoma cell line), MESSA/DX5 (multidrug resistant subline of MES-SA), HCT-15 (human colorectal adenocarcinoma cell line), HCT15/CL02 (multidrug resistant subline of HCT15). The MeOH extract was fractionated into four portions by serial solvent partition, ie., methylene chloride (CH2Cl2) soluble part, ethylacetate (EtOAc) soluble part, n-butanol (BuOH) soluble part and remaining water layer. Among them, the $CH_2Cl_2$ soluble part of the extract exhibited a most potent inhibition on the proliferation of tested tumor cell lines. Bioassay-guided fractionation of the $CH_2Cl_2$ soluble part led to the isolation of five diarylheptanoid and two flavonoid constituents, i. e., galangin (1), 7-(4"-hydroxy-3"-methoxyphenyl)-1-phenylhept-4-en-3-one (2), 1,7-diphenyl-5-hydroxy-3-heptanone (3), trans,trans-1-(3'-methoxy-4'-hydroxyphenyl)-7-phenyl-5-ol-4,6-dien-3-heptanone (4), 5-methoxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone (5), kaempferide (6), 5-hydroxy-7-(4"-hydroxy-3"-methoxyphenyl)-1-phenyl-3-heptanone (7). Structures of the isolated active components (1 - 7) were established by chemical and spectroscopic means.

• Journal of Nutrition and Health
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• 제32권8호
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• pp.946-956
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• 1999

The iron nutritional status of 328(20-70 years old) women in Seoul and Puyo areas was evaluated using a dietary information and a measurement of hematological indices. The serum iron was measured by Red Blood Cell(RBC), Hemoglobin(Hb), Hematocrit(Hct), Serum Iron(S-Fe), Total Iron Binding Capacity (TIBC), Transferrin Saturation(TS) and Serum Ferritin(SF) and was analyzed with the information obtained by interviews which included socio-demographic variables and the dietary pattern of the subjects. The mean Hb was 12.6 $\pm$ 1.21g/dl(Seoul : 12.4 $\pm$ 1.14g/dl, Puyo : 13.2 $\pm$ 1.28g/dl), Hct was 38.7 $\pm$ 3.84%(Seoul: 37.5 $\pm$ 3.28%, Puyo: 4.19 $\pm$ 3.48%), S-Fe was 76.8 $\pm$ 31.49ug/dl(Seoul: 68.8 $\pm$ 27.3ug/dl, Puyo: 78.8 $\pm$ 32.3ug/dl) and TIBC was 277.7 $\pm$ 86.15ug/dl(Seoul: 354.1 $\pm$ 129.8ug/dl, Puyo: 259.0 $\pm$ 59.55ug/dl). The mean Ts(%) was 30.9 $\pm$ 17.9% (Seoul: 21.7 $\pm$ 10.52%, Puyo: 33.2 $\pm$ 18.68%) and the level was significantly higher in Puyo women(p<0.05). The mean SF was 45.4 $\pm$ 46.21ng/ml(Seoul: 53.6 $\pm$ 50.21ng/ml, Puyo: 36.1 $\pm$ 39.83ng/ml). The Red Blood Cell(RBC) showed a negative correlation with age and Hb, Hct showed a negative correlation with education and income level. However TIBC showed a and total energy expenditure per day. Energy, protein, carbohydrate, iron and vit.C intake showed a positive correlation with Hb and Hct. In particular, heme iron showed a positive correlation with RBC and nonheme iron of Hb, income level and iron intake affected on the level of Hct. In Seoul women, the age of menarche affected on the level of TIBC, total energy expenditure affected on the level of Hct. In Seoul women, the age of menarche affected on the level of TIBC, total energy expenditure affected on the level of S-Fe.

• Biomolecules & Therapeutics
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• 제15권3호
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• pp.150-155
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• 2007

In the present study, we have tested the effect of dioleoyl phosphatidic acid (PA) on intracellular $Ca_{2+}$ concentration ($[Ca^{2+}]_{i}$) in two human colon cancer cell lines (HCT116 and HT29). PA and lysophosphatidic acid (LPA), a bioactive lysolipid, increased $[Ca^{2+}]_{i}$ in both HCT116 and HT29 cell lines. Increases of $[Ca^{2+}]_{i}$ by PA and LPA were more robust in HCT116 cells than in HT29 cells. A specific inhibitor of phospholipase C (U73122), however, was not inhibitory to the cell responses. Pertussis toxin, a specific inhibitor of $G_{i/o}$ type G proteins, however, had an inhibitory effect on the responses except for an LPA-induced one in HT29 cells. Ruthenium red, an inhibitor of the ryanodine receptor, was not inhibitory on the responses, however, 2-APB, a specific inhibitor of inositol 1,4,5-trisphosphate receptor, completely inhibited both lipid-induced $Ca^{2+}$ increases in both cell types. Furthermore, by using Ki16425 and VPC32183, two structurally dissimilar specific antagonists for $LPA_{1}/LPA_{3}$ receptors, an involvement of endogenous LPA receptors in the $Ca^{2+}$ responses was observed. Ki16425 completely inhibited the responses but the susceptibility to VPC32183 was different to PA and LPA in the two cell types. Expression levels of five LPA receptors in the HCT116 and HT29 cells were also assessed. Our data support the notion that PA could increase $[Ca^{2+}]_{i}$ in human colon cancer cells, probably via endogenous LPA receptors, G proteins and $IP_{3}$ receptors, thereby suggesting a role of PA as an intercellular lipid mediator.

• IMMUNE NETWORK
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• 제12권2호
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• pp.66-69
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• 2012

The immunological death induction by EY-6 on the human tumor cell lines was screened. Human colon carcinoma (HCT15, HCT116), gastric carcinoma (MKN74, SNU668), and myeloma (KMS20, KMS26, KMS34) cells were died by EY-6 treatment with dose-dependent manner. CRT expression, a typical marker for the immunological death, was increased on the EY-6-treated colorectal and gastric cancer cells. Interestingly, the effects on the myeloma cell lines were complicated showing cell line dependent differential modulation. Cytokine secretion from the EY-6 treated tumor cells were dose and cell-dependent. IFN-${\gamma}$ and IL-12 secretion was increased in the treated cells (200% to over 1000% of non-treated control), except HCT116, SNU668 and KMS26 cells which their secretion was declined by EY-6. Data suggest the potential of EY-6 as a new type of immuno-chemotherapeutics inducing tumor-specific cell death. Further studies are planned to confirm the efficacy of EY-6 including in vivo study.

• Archives of Pharmacal Research
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• 제21권2호
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• pp.147-152
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• 1998

Cinnamaldehydes and related compounds were synthesized from various cinnamic acids based on the $2^{I}$-hydroxycinnamaidehyde isolated from the bark of Cinnamomum cassia Blume. The cytotoxicity to human solid tumor cells such as A549, SK-OV-3, SK-MEL-2, XF498 and HCT15 were measured. Cinnamic acid, cinnamates and cinnamyl alcohols did not show any cytotoxicity against the human tumor cells. Cinnamaldehydes and realted compounds were resistant to A549 cell line up to 15 .mu.g/ml. In contrast, HCT15 and SK-MEL-2 cells were much sensitive to these cinnamaidehyde analogues which showed $ED{50} values 0.63-8.1{\mu}g/ml.$Cytotoxicity of the saturated aldehydes was much weak compared to their unsaturated aldehydes. From these studies, it was found that the key functional group of the cinnamaldehyde-related compounds in the antitumor activity is the propenal group.p.

• 한국식품영양학회지
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• 제27권3호
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• pp.406-413
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• 2014

The objective of this study was to identify the nutritional risk factors by blood analysis, in 1,083 preschool children of age 3 to 6 years. The frequency of anemic children was 7.3% with Hb<11.1 g/dL, 29.9% with ferritin<20 ng/mL, and 16.7% with transferrin Fe saturation(%)<15%. The prevalence of anemia in these children were 12.8% for those with MCV<79 fL, and it was 71% for those with TIBC> $400{\mu}g/dL$. Serum ferritin concentration was 20 ng/mL in the normal children. Thirty two percent of the children had anemia with Hb<12 g/dL, which is below the normal range of Hb. Exactly 15.4% of the children had serum Fe concentration of $60{\mu}g/dL$. The transferrin Fe saturation of the children (16.3%) was >15%. The serum ferritin concentration showed low correlations with Hb, Fe, transferrin Fe saturation, and MCV. The transferrin Fe saturation, higher Hb concentration, MCV, and Hct values were increased significantly. Consequently, iron-deficiency anaemia was thus defined as having Hb concentration <12 g/dL accompanied by ferritin concentration <20 ng/mL or Hct <33%.

• The Korean Journal of Physiology
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• 제15권1호
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• pp.53-59
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• 1981

In the present study, an effort was directed to elucidate the effect of osmolality on the absorption of ethanol in rabbits. A single dose of 13.67 ml(2. 16 gm ethanol/kg BW) of hypo-, iso-hyphen and hypertonic ethanol per kg BW was administered into the stomach to albino rabbits and the experiment was performed at 30 th, 60 th and 120 th minute. The blood ethanol level was determined by the method of Williams et al, and hematocrit(Hct) was determined by the conventional Hct centrifuge and reader. The results are summarized as follow. The blood ethanol level showed the highest value at 60 min after the ethanol ingestion in the hypo- and isotonic groups, $171.3{\pm}13.3\;mg%$ and $204.5{\pm}23.0\;mg%$, respectively, but in the hypertonic group, the highest value was observed at 120min after the ingestion. The absorption rate of ethanol between 0 to 30 min after the ingestion of hypo- and isotonic ethanol was $88.54{\pm}12.04$ and $94.73{\pm}8.33\;mg/min$, respectively, but a decreased value of $44.72{\pm}6.69\;mg/min$ was noted after hypertonic ethanol ingestion comparing with hypo- and isotonic groups, The Hct value after hypo- and isotonic ethanol ingestion was decreased at 30 min but returned to the control level at 120 min. In contrast with hypo- and isotonic ethanol ingestion, hypertonic ethanol ingestion produced an increase of the Hct value at 30 min and returned to the control level at 120 min. The heart rate was increased but the respiratory rate was decreased after ethanol ingestion regardless of the osmolality.

• 한국입자에어로졸학회지
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• 제8권1호
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• pp.9-15
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• 2012

Three different commercial particle counters were used to measure the PM2.5 particles in this study. An Aerosol Spectrometer (AS) 1.109 model of Grimm and a Particle Sensor (PS) 3030 model of HCT were compared with an Aerodynamic Particle Sizer (APS) 3321 model of TSI. The responses of these instruments were compared for four sizes ($1.0{\mu}m$, $1.5{\mu}m$, $2.0{\mu}m$ and $2.5{\mu}m$) of polystyrene latex (PSL) particles and indoor air particles of the office room. The mode diameter, particle size distribution and total particle number concentration of PSL particles were measured by each instrument. In the office room, the total particle number concentration was measured for 25 minutes. In results of particle size distribution and mode diameter, the APS 3321 (52 size-channels) was more accurate than the AS 1.109 (31 size-channels) and PS-3030 (10-szie channels) since the APS has more number of size-channels than the other instruments. However, AS 1.109 and PS-3030 provided similar results of total particle number concentration to those from the APS 3321. In results of office room test, there were no significant difference from each instrument similar to results of PSL test.

• 동의신경정신과학회지
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• 제15권1호
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• pp.65-76
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• 2004

Alzheimer's disease(AD) is a geriatric dementia that is widespread in old ages. In the future AD will be the largest problem in public health service. From old times, much medicines have been used for treatment of dementia, but there is no medicine having obvious effects. AD is one of brain retrogression disease. So we studied on herbal medicine that have a relation for brain retrogression. From old times, in oriental medicine, senile disease such as dementia and AD is treated by exclusion of Tan(痰). But Vascular Dementia(VsD) is due to YuXue(瘀血). So in recent studies, Hua Xue Hua Yu(活血化瘀) medicine is used for precautionary and medical treatment. We studied on the effects for anti-Alzheimer in pCT105-induced neuroblastoma cell lines by Hyeolbuchukeo-tang(HCT). As the results of this study, in HCT group, the apoptosis in the nervous system is inhibited, the repair against the degeneration of Neuroblastoma cells by CT105 expression is promoted. These results indicate that HCT possess strong inhibitory effect of apoptosis in the nervous system and repair effect against the degeneration of neuroblastoma cells by CT105 expression.