• Journal of Plant Biotechnology
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• v.43 no.2
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• pp.157-163
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• 2016

This study was conducted to investigate the potential use of New Zealand spinach (Tetragonia tetragonioides) as a new vegetable crop which will be cultivated in salt-affected soils such as reclaimed areas. New Zealand spinach ecotypes native to Korea were collected across the Southern, Western and Eastern seashore regions of the Korean peninsula, among which fifty-five accessions were later further propagated and evaluated genetically by using an AFLP (amplified fragment length polymorphism) marker. Based on the AFLP analysis performed to uncover the genetic diversity of the collected ecotypes, enzymatic cleavage of the extracted DNA was implemented based on 12 EcoRI and MseI combinations. A total of 1,279 alleles (107 alleles per EcoRI and MseI enzyme combination) were successfully amplified, among which 62 alleles per enzyme combination were polymorphic (58%). The AFLP analysis indicated that the rate of genetic dissimilarity was 29% among the New Zealand spinach collections, which were clustered into the 7 genetic diversity group. This is the first report on the genetic variation in the genus Tetragonia, and the basic information can be applied to select parental lines for enhancing the segregation spectrum of the new halophytic vegetable plant grown in salt-affected areas.

• Journal of Wetlands Research
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• v.22 no.2
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• pp.66-72
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• 2020

The coastal sand dunes have transitional characteristics of marine and inland ecosystems, and they have abundant biodiversity. This study investigated flora and vegetation and analyzed the landscape structure at seven beaches in Busan metropolitan city. As a result, the vascular species of the coastal sand dunes in Busan were identified as 178 taxa of 140 families. Vegetation landscape was damaged to the extent that no natural vegetation was found in 4 of 7 beaches (57%). The coastal dune vegetation was dominated by the Carex pumila community and Carex kobomugi community. The halophyte and invasive alien species were classified into 18 taxa (10% of all species) and 40 taxa (22% of all), respectively. The vegetation that made up the largest area was the Phragmites communis community of the salt marsh, and the species number of halophyte increased as the wetland area increased. This means that the maintenance and management of salt marshes is important to conserve the unique plant diversity of the region.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.394-409
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• 1994

The symbiotic activities of arbuscular mycorrhizal fungi(AMF) such as spore density, symbiotic intensity and vesicle density, phytomasses of higher plants such as Calamagrostis epigeios, Imperata cylindria, Artemisia scoparia, Aster tripolium and Sonchus brachyotus and seasonal change of the AMF activities, electric conductivity and zinc contents in plant and soil were determined in the rhizospheres of higher plants at abandoned old coastal reclaimed lands, where constructed in 12 and 30 years ago. If plants of reclaimed land classified to salinity, symbiotic activities of AMF were high in order of obligate halophyte, facultative halophyte and glycophyte. Also, those plants classified to life form, symbiotic activities of AMF were high in order of annual, biennial and perennial plants. Seasonal variation of spore density, one of symbiotic activities showed that the plateau density maintained continuously from the end of growing season of the higher plants to next spring. For this reason, it regarded that reproduction of AMF spore would be formed in autumn, when the higher plants will be developed. Seasonal change of symbiosis intensity, other symbiotic activities, however, showed that the highest symbiosis intensity occurred in spring and summer but the lowest in autumn. In relationships among symbiotic activities, spore density was directry proportional increase of symbiosis intensity. Moreover, phytomass of higher plants also was directly proportional to increase the spore density as well as symbiosis intensity. Vesicle density, however, did not any correlation with the phytomass, spore density and symbiosis intensity. From these results, it can know that both spore density and symbiosis intensity are strongly possible to use as the measure of symbiotic activity owing to symbiosis of tho-AMF, the more absorption of zinc by the higher plants carried out the less concentration of zinc in the soil.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.5
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• pp.387-394
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• 2001

Blackgram (Vigna mungo) is more salt tolerant than mungbean (Vigna radiata). This study was initiated to know whether the accumulation pattern of mineral ions in different plant parts plays a significant role in the differences in salt tolerance between the two Vigna species. Different mineral ions, viz. N, Cl, Na, K, Mg and Ca in different organs of two varieties of each of blackgram- Barimash-l (susceptible one) and Barimash-2 (tolerant one), and mungbean-Barimung-3 (tolerant one) and Barimung-4(susceptible one), were analyzed after growing with 0, 50, 75 and 100 mM NaCl solutions. The two crops showed a decreased but similar pattern of total N accumulation under saline conditions. The tolerant variety of both the crops showed a less reduction in total N than the susceptible one. Leaves showed the maximum while stem the minimum N, irrespective of levels of salinity. C $l^{[-10]}$ and N $a^{+}$ accumulation increased with the increasing salinity levels. Interestingly, similar to a halophyte, the salt tolerant blackgram exhibited conspicuously higher amount of N $a^{+}$ in the shoot than the salt-susceptible mungbean. However, the tolerant varieties showed less amount of N $a^{+}$ than the susceptible one, especially in blackgram. Seeds of both Vigna spp. accumulated the minimum amount of N $a^{+}$ than other plant parts. $K^{+}$ accumulation decreased by salinity in most of the plant parts, except seeds. Blackgram showed larger reduction in K than mungbean. The $Mg^{++}$ increased in leaves, petioles and stem by salinity while decreased in the roots, podshells and seeds in both the crops. Salinity increased $Ca^{++}$ accumulation in all plant-parts except roots of both Vigna spp. Apparently, the leaves of mungbean accumulated higher concentration of $Ca^{++}$ than blackgram. Varietal differences in the accumulation pattern of $K^{+}$, $Mg^{++}$ and $Ca^{++}$ were not clear. It was concluded that blackgram, presumably, possesses a similar salt tolerance mechanism to halophyte, and the pattern of accumulation of mineral ions in blackgram and mungbean was not fully ascribed to the differences in salinity tolerance between the two Vigna species.gna species.ies.s.ies.

• Weed & Turfgrass Science
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• v.2 no.3
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• pp.260-266
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• 2013

This study was conducted to investigate changes of vegetation and soil characteristics to tidal land of Saemangeum reclaimed land from 2010 to 2012. Soil salinity was 0.16-22.3 dS $m^{-1}$ in the first survey, while the three years later, it was decreased to 0.12-4.22 dS $m^{-1}$. Vegetations were classified as 6 families and 26 species but it was increased to 8 families and 31 species after three years. Numbers of average species in survey site were increased from 7.1 species to 10.6 species. Numbers of vegetation were increased at each survey sites except for survey site 7 : there was decreased halophyte according to decrease in the soil salinity. Biomass production was increased at low production site, and showed highest production in area of dominant vegetation as Phragmites communis. Simpson's dominance ratio(SDR) of main vegetation as Phragmites communis, Calamagrostis epigeios were increased but Suaeda maritima, Salicornia europaea, Puccinellia nipponica and Zoysia sinica were decreased.

• Ocean and Polar Research
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• v.38 no.2
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• pp.115-128
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• 2016

This study examined the relationship between macrobenthic distribution patterns and environmental factors in salt marsh vegetation in Donggeomdo, Ganghwa on the west coast of Korea. Nine stations were fixed on a transect across the salt marsh vegetation, and field sampling was carried out monthly from July 1997 to June 1998. A total of 38 species of macrobenthos were recorded: each of faunal groups, 13 (34.2%) Arthropoda, 12 (31.6%) Polychaeta, 8 (21.1%) Mollusca, and 5 (13.2%) others. The mean density was $2,659individuals/m^2$, with a mean biomass of $178.6gWWt/m^2$. Mollusca dominated in terms of abundance and biomass, with a mean density of $2,172individuals/m^2$ (81.7%) and a mean biomass of $131.9gWWt/m^2$ (73.9%). The number of species decreased in winter (January-February), while mean density increased in the spring (May-June). The biomass was relatively in Summer and Fall (July-November), than any other season. The number of species was high in pure stands of Suaeda japonica in the lower salt marsh vegetation, and the mean density and biomass were high in mixed halophyte communities in the middle salt marsh vegetation. Two Mollusca, the bivalve Glauconome chinensis and gastropod Assiminea lutea, were dominant. The densities of these two species were high in mixed halophyte communities in the middle salt marsh vegetation. Non-metric multi-dimensional scaling (nMDS) showed that the study area could be divided into four groups corresponding to the vertical distribution of tidal levels and halophytes. Spearman's rank correlation revealed that the distribution patterns and community structure of macrobenthos were related to environment variables such as salinity of the substrates, exposure time, and grain size compositions of the sediment in the salt marsh vegetation. Particularly, the distribution and density of some dominant species showed differences along the vertical distributions of halophytes.

• Korean Journal of Environment and Ecology
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• v.30 no.5
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• pp.810-820
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• 2016

This study is focused on the investigation of the genes which are induced by various stresses of the halophyte Salicornia herbacea. One of the factors influencing in the germination of Salicornia herbacea is salt stress. The highest germination rate was found in the condition without NaCl, and the upper limit of the NaCl concentration for the germination of Salicornia herbacea was 7%. The optimal temperature of $20^{\circ}C$showed a germination rate of 98%. Among genes induced by stress the 2CysPrx gene was cloned and analyzed for this study. The 2CysPrx gene has two cysteine conserved residues and is composed of 275 amino acids with molecular weight of 30.1kDa. The 2CysPrx gene appeared to be one copy in the genome and consists of 6 introns and 7 exons. Quantitative real-time PCR revealed that the highest transcription rate induced by NaCl and $H_2O_2$ appeared to be at the concentration of 3.5% NaCl and 40mM $H_2O_2$, respectively. The amount of transcript induced by high temperature($40^{\circ}C$) and $75{\mu}M$ of ABA was respectively highest. The gene at low temperature ($4^{\circ}C$) appeared not to be expressed. We are conducting to clone other peroxyredoxin genes induced by various environmental stresses.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.10
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• pp.1460-1466
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• 2012

The purpose of this study was to evaluate plasticizer pollution levels in mudflat solar salt, salt water and sea water of the nationwide saltpan. Also, it was analyzed the relationship between DEHP in solar salt and PVC mat following private period. DEP (ranging from 0.00 to 1.55 ppb), DIBP (0.00~2.38), DBP (0.00~4.90), DEHA (0.00~2.32), and BBP (0.00~1.45) in solar salt were shown a extremely low concentrations, and DEHP was present a concentrations ranging from 0.00 to 268.5 ppb in solar salt. Further, DMP, DPrP, DNPP, DNHP, and DCHP were not detected in all solar salt. Phthalate in sea water and salt water was present a infinitesimal amount levels. DEHP levels in sea water and salt water were not shown a high risk levels ranging from 0.00 to 3.4 ppb, and from 0.00 to 21.4 ppb, respectively. As expected in PVC mat of nationwide saltpan, the correlation between DEHP in solar salt and PVC mat private period showed a low positive correlation coefficient ($r^2$=0.0362).

• Journal of Life Science
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• v.23 no.2
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• pp.182-189
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• 2013

Halophytes are unique land plants that are capable of thriving in a high-salt environment. They are attracting public attention due to their ability to synthesize bioactive substances such as UV protectants or antioxidizing agents. To achieve unaffected growth under high salinity, halophytes may take advantage of the activities of cell growth factors such as expansins. Expansins are well-known cell wall proteins that are responsible for cell enlargement. They loosen cell walls, thereby contributing to actual plant growth. This study aimed to identify positive roles of expansins in the growth of halophytes. Three expansin cDNA clones were isolated from seedlings of Suaeda japonica. Comparing the deduced amino acid sequences of the expansin genes of S. japonica with those of other plant species suggested that the cDNA clones isolated from S. japonica belong to the EXPA (${\alpha}$-expansin) gene family. A phylogenetic tree based on the deduced amino acid sequences revealed that the expansins of S. japonica share a close evolutionary relationship with those of strawberry (Fragaria ananassa) and jujube (Ziziphus jujuba), both of which are woody dicots. SjEXPAs did not show any remarkable change in the gene expression level in different NaCl concentrations, providing a clue to the unaffected seedling growth of S. japonica in a high-salt environment. In conclusion, the present study presents the first report of expansin genes from halophytes and suggests a putative role for these genes in plant growth under high salinity.

• The Journal of the Convergence on Culture Technology
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• v.5 no.4
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• pp.373-378
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• 2019

In the marine area, the salt concentration in the soil increases, and the inland heavy metal pollution increases the damage of plants. In the inland industrial development area, researches on the genetic resources of plants together with the heavy metal accumulation of Co, Ni, Zn, and so on are required. Both of these problems have caused scientists to work hard to find plants that are likely to cause stress in plant roots. In this study, seeds of Arabis stelleri var. japonica collected near the shore were used for germination. The growth and development and tolerance of both Arabis and Arabidopsis seeds were investigated under laboratory culture conditions. As a result, Arabis showed resistance about 3 times in 250 mM nickle and cobalt, and more than 4 times in 1 mM zinc when compared to Arabidopsis. The tolerance of Arabis to Na salts increased by 20% or more at 50 mM concentration and Arabis was resistant to heavy metals and salt concentration. The accumulation of Na ions in the body was measured as a preparation for studying the intracellular mechanism. As a result, it showed a further decrease in resistance to ground water roots. It is considered that the activity of the exporting gene is important rather than the mechanism of accumulation.