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http://dx.doi.org/10.13047/KJEE.2016.30.5.810

Molecular Isolation and Characterization of the 2CysPrx Gene from Salicornia herbacea  

Kim, Suk-Kyu (Tidal Flat Research Center-National Institute of Fisheries Science)
Chung, Sang Ok (Tidal Flat Research Center-National Institute of Fisheries Science)
Na, Gil-Jong (Dept. of Biology, Kunsan Nat'l Univ.)
Publication Information
Korean Journal of Environment and Ecology / v.30, no.5, 2016 , pp. 810-820 More about this Journal
Abstract
This study is focused on the investigation of the genes which are induced by various stresses of the halophyte Salicornia herbacea. One of the factors influencing in the germination of Salicornia herbacea is salt stress. The highest germination rate was found in the condition without NaCl, and the upper limit of the NaCl concentration for the germination of Salicornia herbacea was 7%. The optimal temperature of $20^{\circ}C$showed a germination rate of 98%. Among genes induced by stress the 2CysPrx gene was cloned and analyzed for this study. The 2CysPrx gene has two cysteine conserved residues and is composed of 275 amino acids with molecular weight of 30.1kDa. The 2CysPrx gene appeared to be one copy in the genome and consists of 6 introns and 7 exons. Quantitative real-time PCR revealed that the highest transcription rate induced by NaCl and $H_2O_2$ appeared to be at the concentration of 3.5% NaCl and 40mM $H_2O_2$, respectively. The amount of transcript induced by high temperature($40^{\circ}C$) and $75{\mu}M$ of ABA was respectively highest. The gene at low temperature ($4^{\circ}C$) appeared not to be expressed. We are conducting to clone other peroxyredoxin genes induced by various environmental stresses.
Keywords
HALOPHYTE; SALT CONCENTRATION; ENVIRONMENTAL STRESS; GERMINATION RATE; PEROXIREDOXIN;
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1 Baier, M and K.J. Dietz(1996) Primary structure and expression of plant homologues of animal and fungal thioredoxin-dependent peroxide reductases and bacterial alkyl hydroperoxide reductases. Plant Mol Biol.. 31: 553-564.   DOI
2 Baier, M and K.J. Dietz(1997) The plant 2-Cys peroxiredoxin BAS1 is a nuclear encoded chloroplast protein. its expressional regulation, phylogenetic origin, and implications for its specific physiological function in plant. Plant J.. 12: 179-190.   DOI
3 Baier, M and K.J. Dietz(1999) Protective function of chloroplast 2-Cysteine peroxiredoxin in photosynthesis. Evidence from transgenic Arabidopsis. Plant Physiol.. 119: 1407-1414.   DOI
4 Bang, M.A., H.A. Kim and Y.J. Cho(2002) Hypoglycemic and antioxidant effect of dietary hamcho powder in Streptozotocin-induced diabetic rats. J. Kor Soc Food Sci Nutr.. 31: 840-846.(in Korean with English abstract)   DOI
5 Breckle, S.W(1990) Salinity tolerance of different halophyte types in N. bassam and M. dambroth, genetic aspects of plant mineral nutrition. Kluwer Academic Publishers Dordrecht.. 167-175.
6 Cha, J.Y., E. Netty, S.G. Kim, J.J. Lee, C.O. Lim, W.S. Chung, K.H. Lee and D.Y. Son(2003) Molecular cloning and characterization of salt-inducible Aldolase from Salicornia herbacea. Kor J. Plant Biotechnol.. 30(4): 323-328.(in Korean with English abstract)   DOI
7 Dietz K.J(2003) Plant peroxiredoxins. Annu Rev Plant Biol.. 54: 93-107.   DOI
8 Dietz, K.J., F. Horling, J. Konig and M. Baier(2002) The function of the chloroplast 2-cysteine peroxiredoxin in peroxide detoxification and its regulation. J Exp Bot.. 53: 1321-1329.
9 Guo, X.L., Y.R. Cao, Z.Y. Cao, Y.X. Zhao and H. Zhang(2004) Molecular cloning and characterization of a stress-induced peroxiredoxin Q gene in halophyte S. salsa. Plant Science.. 167: 969-975.   DOI
10 Han, S.K and S.M. Kim(2003) Antioxidative effect of Salicornia herbacea L. grown in closed sea beach. j .Kor Soc Food Sci Nutr .. 32: 207-210.(in Korean with English abstract)   DOI
11 Horling, F., P. Lamkemeyer, J. Konig, I. Finkemeier, A. Kandlbinder, M. Baier and K.J. Dietz(2002) Divergent light-, ascorbate- and oxidative stress-dependent regulation of expression of the peroxiredoxin gene family in Arabidopsis thaliana. Plant Physiol.. 131: 317-325.
12 Jang, H.H., S.Y. Kim and S.Y. Lee(2006) Oxidative stress-dependent structural and functional regulation of 2-cysteine peroxiredoxins in eukaryotes including plant cells. Korean J. Plant Biotech.. 33(1): 1-9.(in Korean with English abstract)   DOI
13 Jeong, J.H., T.K. Kim, W.Y. Choi, N.H. Baek, C.H. Yang, D.H. Kim, S. Kim, Y.D. Kim, S.B. Lee, K.B. Lee, K.H. Park and K.M. Cho(2013) Optimum salinity concentration and nitrogen fertilization for Salicornia herbacea growth in reclaimed land. Korean J. Intl Agri.. 25(1): 62-67.(in Korean with English abstract)   DOI
14 Jo, Y.C., K.S. Lee, S.M. Chon and D.S. Byun(2002) Characteristics of growth and germination of S. herbacea L. for the soil salinity and manure condition. Korean J. Medicinal Crop Sci.. 10: 100-108.(in Korean with English abstract)
15 Kang, B.H and S.I. Shim(1998) Screening of saline tolerant plant and development of biological monitoring technique for saline stress. Survey of vegetation in saline region and determination of saline tolerance of the plant species of the region. Korean J. Environ Agriculture.. 17(1): 26-33.(in Korean with English abstract)
16 Kim, H.S., J.W. Park, Y.J. Lee, G.W. Shin, I.B. Park and Y.C. Jo(2009) The amino acid content and antioxidant activities of glasswort. Kor J Food Preserv.. 16: 427-434.(in Korean with English abstract)
17 Kim, J.A., Y.S. Choo, I.J. Lee, J.J. Bae, I.S. Kim, B.H. Choo and S.D. Song(2002) Adaptations and physiological characteristics of three chenopodiaceae species under saline environments. Korean J. Ecology.. 25(3): 171-177.(in Korean with English abstract)
18 Kinzel, H(1989) Calcium in the vacuoles and cell wall of plant tissue. Flora.. 182: 99-125.   DOI
19 Kim, S., T.K. Kim, J.H. Jeong, C.H. Yang, J.H. Lee, W.Y. Choi, Y.D. Kim, S.J. Kim and K.Y. Seong(2012a) Characteristics of vegetation on soils having different salinity in recently reclaimed Saemangeum in region of Korea. Korean J. Weed Sci.. 32(1): 1-9.(in Korean with English abstract)   DOI
20 Kim, S.Y., Y.J. Jung, M.R. Shin, J.H. Park, G. M. Nawkar, P. Maibam, E.S. Lee, K.S. Kim, S.K. Paeng, W.Y. Kim, K.O. Lee, D.J. Yun, C.H. Kang and S.Y. Lee(2012b) Molecular and functional properties of three different peroxiredoxin isotypes in Chinese cabbage. Mol Cells.. 33: 27-33.   DOI
21 Sambrook, J and D.W. Russell(2000) Molecular cloning: A laboratory manual, Ed 3. Cold Spring Harbor Laboratory Press. Cold Spring Harbor. New York.
22 Konig, J., M. Baier, F. Horling, U. Kahmann, G. Harris, P. Schurmann and K.J. Dietz(2002) The plant-specific function of 2-Cys peroxiredoxin-mediated detoxification of peroxides in the redox-hierarchy of photosynthetic electron flux. Proc Natl Acad Sci USA.. 99: 5738-5743.   DOI
23 Laura, B.V., N. Eusebio, S. Francisca and J.J. Lazaro(2004) Cloning and characterization of a 2-Cys peroxiredoxin from Pisum sativum. J Experi Botany.. 55: 2191-2199.   DOI
24 Park, J.C., Y.O. Jeong, Y.S. Kim and M.R. Huh(2004) Research on transplant possibility of glasswort(Salicornia herbacea) into new reclaimed land. KSPP Envir.. 7: 59-63.(in Korean with English abstract)
25 Tovar-Mendez, A., M.A. Matamoros, P. Bustos-Sanmamed, K.J. Dietz, J.C. Francisco, N. Rouhier, S. Sato, S. Tabata and M. Becana(2011) Preoxiredoxins and NADPH-dependent thioredoxin systems in the model legume Lotus japonicus. Plant Physiol.. 156: 1535-1547.   DOI