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Evaluation of Cytotoxicity, Antimicrobial and Antioxidant Enzyme Activity of Diploid and Tetraploid Platycodon grandiflorum

  • Boo, Hee-Ock;Kim, Young-Sun;Kim, Hag-Hyun;Kwon, Soo-Jeong;Woo, Sun-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.2
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    • pp.239-247
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    • 2015
  • This experiment was conducted to obtain the have higher contents of pharmaceutical constituents as well as higher yield from colchicine induced diploid and tetraploid extracts of Platycodon grandiflorum. In order to determine the biological activity, this study was focused to evaluate the cytotoxicity, antimicrobial on the bronthus disease bacteria, antioxidant enzyme activity of diploid and tetraploid extracts in P. grandiflorum. The activities of antioxidant enzyme according to different solvent extracts were measured as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The cytotoxicity of methanol extracts of P. grandiflorum showed significant differences between tetraploid and diploid. That is, the cytotoxic effect against human cancer cell was higher in tetraploid than in diploid. At all extracts concentration, tetraploid samples showed high toxicity and the $IC_{50}$ (concentration causing 50% cell death) value showed the highest on HCT-116 cell ($105.91{\mu}g/mL$), and exhibited significant activity against the Hep 3B cell ($140.67{\mu}g/mL$), SNU-1066 cell ($154.01{\mu}g/mL$), Hela cell ($158.37{\mu}g/mL$), SNU-601 cell ($182.67{\mu}g/mL$), Calu-6 cell ($190.42{\mu}g/mL$), MCF-7 cell ($510.19{\mu}g/mL$). Antimicrobial activities of diploid P. grandiflorum were relatively low compared to tetraploid P. grandiflorum on most of the bacterial strains. In tetraploid P. grandiflorum, K. pneumoniae showed the clear zone formation (18~19 mm) of growth inhibition, followed by the clear zone formation of 13~15 mm on C. diphtheria and S. pyogenes. The antimicrobial activities in diploid P. grandiflorum were the highest on K. pneumonia (14~15 mm), and showed the clear zone formation of 11~12 mm on C. diphtheria and 12~13 mm on S. pyogenes. The antimicrobial activity is thought to look different depending on the bacterial strains and the polyploidy of P. grandiflorum. The root extract of P. grandiflorum had the highest (97.2%) SOD enzyme activity in ethyl acetate partition layer of tetraploid while water partition layer of diploid showed the lowest (48.6%) SOD enzyme activity. The activity of CAT showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all partition layers except butyl alcohol. The activities of APX and POD showed higher values in the root of tetraploid than in the diploid of P. grandiflorum in all fraction solvents except water layer. These results indicate that the tetraploid P. grandiflorum can be used as a source for developing cytotoxic agent and antimicrobials which can act against bronchus diseases bacterial strains.

Making Techniques of Hight Quality Powder in Sweetpotato (고구마 고품질 분말 제조기술)

  • Lee, Joon-Seol;Ahn, Young-Sup;Kim, Hag-Sin;Chung, Mi-Nam;Jeong, Byeong-Choon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.198-203
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    • 2006
  • This study was conducted to select the temperature for hot air drying method, cutting method, and solution that inhibits enzymatic browning in making high quality powder from sweetpotato roots. It was observed that $70^{\circ}C$ was the best temperature for air drying method wherein the dry matter rating, dry matter time, flour yield and chemical component were considered. The proper cutting thickness should be 2.0 mm considering the vitamin C content and sensory evaluation of sweetpotato flour. Also, 1% vitamin C solution inhibited enzymatic browning of sweetpotato.

Effective Weed Control in Paddy Field Simultaneously Dominated by Herbicide-Resistant Weeds, Echinochloa oryzoicola, Monochoria vaginalis and Scirpus juncoides (제초제 저항성 잡초 강피, 물달개비, 올챙이고랭이 동시 우점한 논에서 효과적인 제초관리)

  • Park, Tae Seon;Cho, Hyeoun Suk;Hwang, Jae Bok;Ku, Bon il;Kim, Hag Sin;Seo, Myung Chul;Park, Hong Kyu;Lee, Keon Hui
    • Weed & Turfgrass Science
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    • v.4 no.3
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    • pp.151-158
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    • 2015
  • This study was conducted to establish the effective weed management methods in rice field simultaneously dominated by the herbicide resistant Echinochloa oryzicola, Monochoria vaginalis and Scirpus juncoides. Herbicides registered for use before transplanting, oxadiazon 12% EC, pyrazolate 36% SC, pretilachlor 14% EC and thiobencarb 50% EC were effective until 0.5 leaf stage of herbicide resistant Echinochloa oryzoides. Herbicides registered for use after transplanting, fentrazamide 1% GR and mefenacet 18% SC were effective until 2 leaf stage of herbicide resistant Echinochloa oryzicola and triafamone 0.98% SC was possible to control up to 4 leaf stage. HPPD inhibitors, benzobicyclon, mesotrione and tefuryltrione SC, were simultaneously effective to SU herbicide-resistant Monochoria vaginalis and Scirpus juncoides. Herbicides registered for use before transplanting, benzobicyclon + oxadiargyl EC out of the tested herbicide was most effective in rice field simultaneously dominated by the herbicide resistant Echinochloa oryzicola, Monochoria vaginalis and Scirpus juncoides. Its effectiveness rises in proportion to flooding duration. Mazosulfuron GR, a herbicides registered for use after transplanting was most effective without phytotoxicity until 60 days after transplanting in rice field simultaneously dominated by the herbicide resistant Echinochloa oryzicola.

Difference of Agricultural Characteristics and Quality with Fertilizer Types in Wheat Cultivation (밑거름 종류에 따른 밀의 농업적 형질 및 품질 차이)

  • Kim, Hag-Sin;Kim, Young-Jin;Kim, Kyong-Hyun;Park, Hyung-Ho;Kang, Chon-Sik;Kim, Kyung-Ho;Hyun, Jong-Nae;Kim, Kee-Jong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.1
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    • pp.15-19
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    • 2013
  • This study was carried out to establish the optimum organic fertilization and microbial compost on wheat cultivation in order to reduce the use of chemical fertilizers and improve the quality of wheat. The tests resulted in a yield of organic fertilization of 2~6% lower than the yield of standard 4.16 Ton/ha (a yield more than that of microbial compost). The recession was not statistically significant. The trial which involved organic fertilizer that had a yield of 800 kg/ha and microbial compost which had a yield of 2,000 kg/ha resulted in 96% yield of standard trial. The quality of flour in the manure was 50% less during the trial and was not making a good result. In protein content and SDS-sedimentation volume, standard trial had the highest yield in test trial (standard > miccompost > organic fertilization). However, Ash content was not statistically significant.

Studies on the Taste of Korean for Cheese (한국인(韓國人)의 치이즈 기호성(嗜好性)에 관한 연구)

  • Kim, Jong Woo;Ko, Keun Hag
    • Korean Journal of Agricultural Science
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    • v.18 no.1
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    • pp.21-32
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    • 1991
  • This experiment was carried out to examine sensory testing for Mozzarella cheese, process cheese, Cheddar cheese and Cheddar cheese made with red pepper, garlic, ginger and welsh onion to develop new cheese varieties which can be prefered by Korean. The chemical composition and sensory testing of cheese were measured. The results were summarized as follows ; 1. Total nitrogen percentages in Cheddar cheese and spiced Cheddar cheeses were similar but those in process cheese and Mozzarella cheese were low. 5% NaCl soluble nitrogen percentages were highest in Cheddar cheese. 5% NaCl soluble nitrogen percentages in each cheese were different. Ripening degree, water soluble nitrogen, TCA soluble nitrogen and SSA soluble nitrogen percentages in each cheese were similiar level. 2. Spiced Cheddar cheeses were more breakdown than other cheese and ${\alpha}_s$-casein breakdowns faster than $\beta$-casein. 3. In the result of sensory evaluation, color score was high in Mozzarella cheese and process cheese. The color score of Cheddar cheese was high in 30's-40's and 50's- 50's. The color score of 10's and 20's was high in Cheddar cheese made with garlic. 4. Odor score was high in Mozzarella cheese and process cheese, too. The odor score of Spiced Cheddar cheeses was high in 10's. 5. Texture score was high in Mozzarella cheese, process cheese and Cheddar cheese. 6. Teste score was high in Mozzarella cheese, process cheese and Cheddar cheese. The taste score of spiced Cheddar cheese was higher in 10's and 20's than that in 30's-40's and 50's-50's.

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Variation of organic matter and rice yield in in continuous forage use of barley and rice straw

  • Ku, Bon-Il;Hwang, Jae-Bok;Choi, In-Bea;Bae, Hui-Su;Kim, Hag-Sin;Park, Tae-Seon;Park, Hong-Kyu;Lee, Geon-Hwi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.184-184
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    • 2017
  • Organic matter is very important and essential factor to maintain productivity of paddy field. But as meat consumption and the demand of grain increase, the demand of forage also increased gradually in Korea. So the amount of organic matter in paddy field have been declined gradually by the reduction of return of rice straw for the forage use for cattle. There is not enough alternative resources for forage, we guess this trend of organic matter decline in paddy field would continue for the time being in Korea. So this study was performed to confirm the variation of organic matter and change of rice yield to select suitable rice cultivars which yield reduction is small in condition of organic matter decrease in paddy field. To confirm the change of rice growth and yield in condition of organic matter decrease in paddy field, we transplanted 10 rice cultivars which yield reduction are small in low fertilizer condition. We transplanted it Buan where double cropping of winter barley continues several years on June 10. Planting density were $70plants/3.3m^2$ Fertilizer amount was $N-P_2O_5-K_2O=9-4.5-5.7kg/10a$ and fertilizer split application of nitrogen was basal-tillering stage-panicle initiation = 50-20-30%. And in the other cultivation management, we observed rice standard culture of NICS. In paddy field where withdraw straws of barley and rice, the organic matter content showed tendency to decrease as the years go on. During rice cultivation season, organic matter decreased little by little, but it increased again after rice harvest season. Rice yield was more in order of Sodami, Chunghaejinmi and Saenuri. We judge that there is an advantage in rice yield of rice cultivars which have later heading date because of suitable ripening temperature. Although Sobibyeo and Shindongjinbyeo showed high yield, head rice yield decreased severely owing to chalky and cracked rice.

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Growth, yield and mineral element responses to temperature in Codonopsis lanceolata

  • Kwon, Soo Jeong;Han, Eun Ji;Moon, Young Ja;Cho, Gab Yeon;Boo3, Hee Ock;Lee, Moon Soon;Woo, Sun Hee;Kim, Hag Hyun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.274-274
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    • 2017
  • Temperature has an adverse effect for potential growth and development of Codonopsis lanceolata. The present study examined the responses of growth, yield and mineral element contents under temperature variables in C. lanceolata. Plant height exhibited the best result as being 2 m or higher the open field culture, while the growth is the most restricted in the $30^{\circ}C$. The open field culture also had the largest number of leaves in the early stage of growth. The number of leaves and the height tended to be increased with the temperature of $20^{\circ}C$ and $25^{\circ}C$. The number of branches showed no significance as 32 branches and 26 branches in open field culture and $20^{\circ}C$, respectively. The smallest number of branches was observed in the $30^{\circ}C$ with 15 branches. The leaf color recorded the lowest value in the open field culture unlike the growth above the ground. But all other temperature had no significance with the leaf color value between 50.0 and 52.6. The elevated temperature induced the thinner and shorter diameter and length of root. However, the root diameter was not significantly changed with the change of 1.8 cm and 2.1 cm at open field culture, with $20^{\circ}C$ and $25^{\circ}C$, respectively. The fresh weights of the primary root and lateral roots were 6.3 g and 3.1 g, respectively, at the high temperature. The result showed the thickening of the lowest part under the ground as restricted. The fresh weights of the main root and lateral root were the highest on the open field culture, 20.6 g and 12.2 g, respectively. The contents of cellulose were the highest as 4.41% in the $20^{\circ}C$, followed by the open field culture, $25^{\circ}C$ and $30^{\circ}C$. The higher temperature reduced the contents of cellulose. Moreover, P showed higher ratio in the high temperature while Ca and Mg exhibited the higher ratio towards the low temperature. On the contrary, minor quantity (less than 1%) of mineral nutrients was observed towards all temperature variables.

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A Case of Obstructive Sleep Apnea Syndrome in Childhood (소아 폐쇄성 수면무호흡증후군 1례)

  • Lee, Seung-Hoon;Kwon, Soon-Young;Lee, Sang-Hag;Chang, Ji-Won;Kim, Jin-Kwan;Shin, Chol
    • Sleep Medicine and Psychophysiology
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    • v.11 no.1
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    • pp.50-54
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    • 2004
  • The obstructive sleep apnea syndrome can occur due to various etiologies in children. In otherwise healthy children, adenotonsillar hypertrophy is the leading cause of childhood obstuctive sleep apnea. Obstructive sleep apnea caused by adenotonsillar hypertrophy can lead to a variety of symptoms and sequelae such as behavioral disturbance, enuresis, failure to thrive, developmental delay, cor pulmonale, and hypertension. So if obstructive sleep apnea is clinically suspected, proper treatment should be administered to the patient after diagnostic examinations. More than 80% improvement is seen in symptoms of obstructive sleep apnea caused by adenotonsillar hypertrophy in children after tonsillectomy and adenoidectomy. However, when it is impossible to treat the patient using surgical methods or residual symptoms remained after tonsillectomy and adenoidectomy, additional treatments such as weight control, sleep position change, and continuous positive airway pressure (CPAP), should be considered. This paper reports a case using weight control and Auto-PAP to control mild sleep apnea and snoring, which in long-term follow-up were not resolved after tonsillectomy and adenoidectomy for severe obstructive sleep apnea.

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Medium Composition and Growth Regulator on Organogenesis Platycodon grandiflorum (Jacq.) A. DC. with Yellow Green Petals ('녹색 꽃잎 도라지'의 기관분화에 미치는 배지조성 및 생장조절제의 영향)

  • Kwon, Soo Jeong;Cho, Kab Yeon;Kim, Hag Hyun
    • Korean Journal of Plant Resources
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    • v.27 no.1
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    • pp.43-50
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    • 2014
  • This study was carried out to determine the optimal medium composition and growth regulators for the micropropagation of Platycodon grandiflorum (Jacq.) A. DC. Nodes containing yellow green petals were used as plant materials to execute the study. The best performance of adventitious root development was found in 1/4 strength of MS basal salt and the growth was satisfactory in the concentration of 1/2 MS medium. The best condition for adventitious root development and growth was observed in the higher concentration (5%) of sucrose and activated charcoal free 1/4MS medium respectively. Adventitious roots were developed at the controlled culture medium at pH 4.8 with a tendency of suppression with higher levels of pH. However, it was prevailed that the development and growth depended on the concentration of agar. The lower concentration of agar (0.4%) was performed better than that of higher concentration (1.2%), whereas the agar concentration (0.4%) showed the best performance for the development and growth of adventitious roots. For the development of shoots containing node, BA combined with IAA was more effective than kinetin with IAA or NAA. The highest shoot development (3.9 shoots per explant) was performed on MS medium supplemented with 0.1 mg/L BA and 0.5 mg/L IAA.

Effects of Onion Kimchi Extract Supplementation on Blood Glucose and Serum Lipid Contents in Streptozotocin-induced Diabetic Rats (양파김치 추출물 투여가 Streptozotocin 유발 당뇨병 흰쥐의 혈당강하 및 혈중지질 함량에 미치는 영향)

  • Yang, Ya-Ru;Kim, Hag-Lyeol;Park, Yang-Kyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.4
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    • pp.445-451
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    • 2008
  • The purpose of this study was carried out to examine the effects of onion kimchi extract supplementation on blood glucose level and serum lipid components in streptozotocin (STZ)-induced diabetic rats for 4 weeks. STZ was administered as a single dose (50 mg/kg BW) to induce diabetes, and the diabetic rats were divided into eight groups (normal, diabetic control, and six treatment groups). The dose of onion kimchi extract 100 (OK-100), 200 (OK-200), and 400 (OK-400) mg/kg/day or quercetin as a main compound of onion 5 (Q-5), 10 (Q-10), and 20 (Q-20) mg/kg/day were orally administered daily to STZ-induced diabetic rats for 4 weeks after STZ injection. The diabetic control rats (465.6 mg/dL) showed significantly higher blood glucose level than the normal rats (76.3 mg/dL) after 4 weeks, but was significantly reduced with onion kimchi extract and quercetin supplementation (p<0.001). Changes in body weight, kidney weight and urine volume were not significantly different in diabetic control rats, and in onion kimchi extract and quercetin treated rats. The serum total cholesterol levels of control were significantly decreased in onion kimchi extract and quercetin supplementation groups, respectively (p<0.001). The blood urea nitrogen level and urinary protein excretion in diabetic rats were not significant different among the groups. These results suggest that onion kimchi extract supplementation in STZ-induced diabetic rats may be a very important factor for the reduction of blood glucose and serum cholesterol profiles.