• 대한화학회지
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• 제32권1호
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• pp.53-59
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• 1988

파라치환된 포타슘 페녹사이드류와 18-crown-6가 이루는 1 : 1 착물 형성상수를 전기 전도도법으로 35${\circ}C$에서 측정하여 용매와 치환기의 변화에 따른 효과를 측정하였다. 18-crown-6와 $K^+$의 착물 형성상수는 $CH_3$OH(DN=19.0) > DMF(26.6) > DMSO(29.8)의 순으로 감소되었다. 이는 전자주게 수가 클수록 $K^+$과 용매와의 상호작용이 커지므로 크라운 에테르와의 착물형성과의 서로 경쟁관계가 되어 $K_f$값이 작아진다고 추정된다. 포타슘 페녹사이드의 치환기가 p-N$O_2$ > H > p-$CH_3$ > p-O$CH_3$의 순으로 $K_f$값이 감소하는 경향을 나타내었다. 이는 전자를 당기는 기는 페녹사이드 음이온이 비편재화되어 안정해지므로 전자를 미는 기에 비해서 $K^+$를 쉽게 내어 놓을 수 있어 18-crown-6와의 착물 형성이 용이하기 때문이라고 생각된다.

• The Korean Journal of Ecology
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• 제24권2호
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• pp.101-109
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• 2001

We created an environmental forest on the basis of ecological design around the incineration plant of Jindo Engineering and Construction Co., Ltd., which is located in Jeongwang-dong, Siheung-si, Kyunggi-do. To get ecological information of this site, physico-chemical properties of soil on salt marsh, which is located close to the syudy site and of forest soil transported from other sites for ecological restoration were analyzed. Texture of salt marsh and transported soils were loam and sandy loam, respectively. pH, organic matter, T-N, available P, and exchangeable K and Na contents of salt marsh and transported forest soils were 6.7 and 5.4, 4.1 and 0.4%, 1.0 and 0.3mg/g, 46.7 and 6.8ppm, 521 and 207ppm, and 3.8 and 0.5mg/g, respectively. Introduced plants were selected among the dominant species of forests and the species composing the potential natural vegetation around the present study site. Those plants were selected again by considering the tolerances to air pollution and to salt, and their availability. Selected trees were Pinus thunbergii, Sophora japonica, Celtis sinensis, Quercus aliena, Q. serrata, Q. dentata, and Q. acutissima. Selected sub-trees were Albizzia julibrissin, Koelreuteria poniculata, and Styrax japonica and shrubs were Rhododendron yedoense var. poukhanense, R. mucronulatum, Callicarpa japonica, Euonymus alatus, E. japonica, and R. schlippenbachii. On the other hand, introduction of herbs was not considered except for Liriope platyphylla, which was ornamentally planted in one site. Planting bed of mound type was adopted to provide the fine drainage system. Mound was designed to furnish litter, A, B, and C layers simuating the profile of forest soil. Slope of mound was mulched by rice straw of 2cm in thickness to prevent for sliding of litter and soil in cases of strong wind or heavy rain. Height of mound was designed to secure more than 1 m by combining A and B layers. Narrow zones, in which mound with stable slope degree cannot be prepared, was designed to equip the standard soil depth with the introduction of stone for supporting. On the other hand, plants with shallow root system were arranged in some zones, in which satisfactory soil depth cannot be ensured. Plants were arranged in the order of tree, sub-tree, and shrub from center to edge on the mound to make a mature forest of a dome shape in the future. Dispersion of plants was designed to be random pattern rather than clumped one. Problems on creation of the environmental forest by such ecological design were found to be management or inspection by non-specialized project operators and inspecting officers, and regulations for construction without ecological background. Alternative plans to solve such problems were suggested.

• 한국환경과학회지
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• 제27권5호
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• pp.333-340
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• 2018

This study aimed to estimate the effect of inoculant application level on chemical composition and bacterial count of fermented chestnut meal (FCM), and its rumen fermentation characteristics. The inoculant contained Lactobacillus acidophilus ($1.2{\times}10^{10}cfu/g$), Bacillus subtilis ($2.1{\times}10^{10}cfu/g$), and Saccharomyces cerevisiae ($2.3{\times}10^{10}cfu/g$). The chestnut meal mixed with molasses, double distilled water, and inoculant at 1 kg, 3 g, 480 mL, and 20 mL ratio for the basal chestnut meal diet. The double distilled water from basal chestnut meal diet was substituted with bacterial inoculant at a level of 0 (Control), 20 (Medium), and 40 mL (High) in the experimental diets. The mixed experimental diets were incubated at $39^{\circ}C$ for 7, 14, and 21 days, respectively. On 7 days of FCM incubation, the contents of crude protein (CP) (quadratic, P=0.043) and neutral detergent fiber (quadratic, P=0.071) decreased by increases of inoculant application levels, whereas bacterial count (quadratic, P=0.065) and rumen $NH_3-N$ (linear, P=0.063) increased. By increases of inoculant application levels on 14 days of FCM incubation, the increases were found on dry matter (DM) (quadratic, P=0.085), CP (quadratic, P=0.059), acid detergent fiber (quadratic, P=0.056), in vitro DM digestibility (linear, P=0.002), rumen total volatile fatty acid (VFA) (linear, P=0.057), and rumen iso-butyrate (linear, P=0.054). However, the decreases were found on bacterial count (linear, P=0.002), propionate (linear, P=0.099), and butyrate (quadratic, P=0.082). On 21 days of FCM incubation, in vitro DM digestibility (linear, P=0.002) and total VFA (linear, P=0.001) increased by increases of inoculant application levels, whereas the contents of CP (quadratic, P=0.034) and neutral detergent fiber (quadratic, P=0.047) decreased. These results indicate that the FCM with a medium level of inoculant application and 14 of fermentation had beneficial effects by increasing DM digestibility and rumen total VFA content, without altering bacterial count.

• 대한예방한의학회지
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• 제4권1호
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• pp.32-50
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• 2000

On the process of research in the plan on oriental medical participation in the industrial health sponsored by BK21 project, we carried out the oriental medical health examination program for workers during former half-year We reached the conclusion as follows, 1. The oriental medical health examination program is contents and formalities that should be determined by present industrial health system, based on the oriental medical system and scholastic character, and included probability of the western and oriental medical cooperation. 2. The oriental medical health examination program can promote capability of individual health management and productive power of workers, and it is capable to manage on the self-conscious symptoms and macroschophically approach to their environment 3. The oriental medical health examination program that we have developed, is flow as questionare, understanding of working environment, information of result and later management. It is composed of three fields as follow , first, use of pulse diagnostic apparatus, understanding of the health promotion life style, and diagnosis of the oriental medical doctor, second, analysis of constitution, third, photographing for understanding of the musculoskeletal disorders, questionare for musculoskeletal self-conscious symptoms, and diagnosis of oriental medical doctor. 4. The oriental medical health examination program that we have developed, progressive from the view point of health, makes the oriental medical doctor's roll more important. It is the first trial at the western and oriental medical cooperation and characterized by excellence about musouloskeletal disorders. But it need to be improved in aspects of time and specialist on the health examination, diagnostic apparatus, control of examinant and later management. So we think that it needs research on the employment of health examination specialist, establishment of later management system, development of significantly diagnosable standard and assessable form on the health examination, and contents of health examination on the western and oriental medical cooperation.

• 한국가금학회지
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• 제32권2호
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• pp.107-112
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• 2005

본 연구는 닭고기의 품질 등급을 설정하기 위한 기초 자료로 이용하기 위하여 시중 도계장을 대, 중, 소로 구분하여 생산 관리 책임자들을 대상으로 닭고기 등급제 적용에 따른 도계장 및 유통업소 및 판매장 조사를 통하여 등급 판정 방법, 표본 수 선발 방법 등을 설정하여 설문지에 의한 방문 조사를 실시한 결과는 다음과 같았다. 도계장 설문 조사에서 등급제 적용시 적용 단위는 대부분의 도계장에서 농가단위 보다는 롯트 단위를 선호하였다. 닭고기 등급제 조사방법은 대부분의 도계장에서 전수 조사보다는 샘플 조사를 선호하였다. 닭고기 등급제 시행 초기에는 도계장에서 요청한 물량에 대해서만 등급 판정을 실시하는 것을 선호하였다. 통닭뿐만 아니라 부분육에 대해서도 등급제를 적용해야 된다는 의견이 많았다. 닭고기 등급제 적용시의 중량의 범위는 대규모 도계장에서는 $8\~12$호에 대해서 등급 판정을 선호하였다. 도계조건에서 실신 시 전압은 대규모의 경우 81V이상의 전압을 주로 사용하였고, 냉각조는 대규모 도계장 일부를 제외하고는 침수식을 사용하였다. 대규모 도계장에서는 닭고기 출하 시 개체 및 박스 포장을 같이 사용하고 있었으나 소규모 도계장은 박스 포장만 사용하였다.

• 한국환경생태학회지
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• 제14권1호
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• pp.38-45
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• 2000

강원도 고성군 죽왕면과 토성면 일대의 소나무군락을 대상으로 1996년 4월에 발생한 산화에 따른 산림환경변화가 토양의 이화학성에 미치는 영향을 구명하기 위하여 비산화지역(NF) 산화후 비벌채지역(FNC), 산화후 벌채지역(FC) 및 산화후 조림지역(FCP)으로 분류하여 토양의 특성을 분석하였다. 전조사지역의 토성은 사질식양토이었고, 토양의 입경조성을 비교하면 비산화지역은 표토의 모래함량이 심토보다 낮았고, 점토함량은 높았지만 산화지역은 모두 표토의 모래함량이 점토에 비해 높았다. 토양공극 분포 중 전공극량은 지역별로 큰 차이가 없었으나, 조공극량과 투수성은 비산화지역 ＞ 산화후 비벌채지역 ＞ 산화후 조림지역 ＞ 산화후 벌채지역 순이었고, 세공극량과 가비중은 반대의 경향을 나타내었다. 이는 산화에 따른 산림환경변화로 지피식생이 제거되어 토양침식이 가속화되면서 토양 내 수분함유능력의 지표인 토양공극과 투수성에 큰 영향을 주면서 토양물리성을 악화시키고 있는 것으로 판단된다. 토양 pH는 비산화지역과 산화후 비벌채지역이 산화후 벌채지역과 조림지역보다 표토 및 심토 모두 높게 나타났다. 유기물함량 및 전질소함량 변화는 표토와 심토 모두 비산화지역＞ 산화후 비벌채지역＞ 산화후 조림지역＞ 산화후 벌채지역 순이었다. 양이온치환용량 및 치환성 양이온($K^{+}$ , $Na^{+}$ /, $Ca^{2+}$, $Mg^{2+}$)함량 변화는 모두 표토가 심토보다 높았으며, 지역별로는 비산화지역＞ 산화후 비벌채지역＞ 산화후 조림지역＞ 산화후 벌채지역 순으로, 이는 산화후 표토층의 침식으로 지력이 악화된 결과로 사료된다.

• Journal of Gastric Cancer
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• 제3권4호
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• pp.186-190
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• 2003

Purpose: The balance between cell proliferation and apoptosis is crucial for homeostatic maintenance in a cell population. Decreased apoptosis or uncontrolled proliferation can lead to cancer. The Fas receptor signal through a cytoplasmic death domain is very important in the apoptotic pathway. To identify the effect of the death domain of the Fas gene in the development and/or progression of gastric cancer, we examined the apoptotic potential of five known Fas mutants detected in gastric cancers. Materials and Methods: A wild-type Fas gene was cloned with cDNA from normal liver tissue and full length Fas was sequenced. Mutants of the gene were generated with sitedirected mutagenesis by using the wild-type gene and specific primers. Wild- and mutant-type genes were transfected to HEK293 cells. Forty-eight hours after transfection the cells were stained with DAPI and cell death was counted under fluorescent microscopy. Results: In wild-type Fas-transfected cells, the percentage of apoptotic cells was $85.9\pm3.6\%$, and significant cell death and classic morphologic signs of apoptosis were observed. However, the percentages of apoptotic cells transfected with N239D, E240G, D244V, and R263H of tumor-derived mutant Fas were $29.5\pm2.08\%,\;28.5\pm3.34\%,\;25.225\pm2.06\%,\;and\;36.625\pm4.49\%$, respectively. Conclusion: These results suggest that inactivation of Fas caused by mutations in the death domain of the Fas gene may be one of the possible escape mechanisms against Fas-mediated apoptosis and that inactivating mutation of the Fas may contribute to the development or progression of gastric cancers.

• Asian-Australasian Journal of Animal Sciences
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• 제14권8호
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• pp.1138-1143
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• 2001

A total of 96 piglets were weaned at 19 and 13 days in Exp. 1 and 2, respectively, and allotted to one of four diets: three with different spray dried plasmas (SPs) and one with hydrolysed casein (HC). SPs were from pigs (SPP), mixed origin (SMP), and mixed origin with standardized level of immunoglobulins (SMPIG). All the diets contained 1.7% total lysine, 25% of the test protein source, 45% corn starch, 15% lactose, 2% sucrose, 7% soybean oil. At d 4 and d 2 in Exp. 1 and 2, respectively, piglets were perorally challenged with $10^{10}$ CFU E. coli K88. Growth performance, immunity, and health condition were measured for 15 days and 14 days in Exp. 1 and 2, respectively. To investigate apparent ileal digestibility and nutrient deposition, all piglets were sacrificed at d 14 in Exp. 2. In 1. 3 piglets died in HC diet and 1 in SPP diet. HC diet showed higher mortality (p<0.01) than other diets. In Exp. 2, no clinical sign of infection was detected, no difference for the content of E. coli K88 was found in feces at 4 and 6 days after the infection, and no E. coli K88 was found in the jejunum at the end of experiment. In both experiments, feed intake was lower for HC diet and ADG was 96, 106, 122 and 155 for HC, SPP, SMP and SMPIG diet, respectively (HC vs others, p<0.05; SMPIG vs other SP, p<0.01). Heal apparent digestibility of nitrogen in sacrificed piglets was higher for HC diet (p<0.05). After the challenge, K88-specific titers in saliva (Exp. 1) and in plasma (Exp. 2) were reduced in SMP and SMPIG. The piglets positive to the adhesion of the used E. coli strain to the intestinal brush borders had a significantly reduced growth (p<0.01) and a higher K88-specific IgA titer in plasma, in comparison with negative ones. This effect was independent of the diet. The data show the relevance of spray dried plasma sources and particularly of SP with standardized level of immunoglobulins for the feeding of early-weaned at the risk of infection by enterotoxigenic bacteria.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.873-882
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• 2008

Eight ileally cannulated pigs (BW $35.9{\pm}0.9kg$) were randomly allotted according to a $4{\times}3$ Latin square design to determine the effects of cellulose, pectin and starch on standardized ileal digestibility (SID) and apparent total tract digestibility (ATTD) of crude protein (CP) and amino acids (AA) as well as on the bacterial AA contribution in feces. The pigs were fed the control diet (20.2% CP, % dry matter (DM)) or one of the three experimental diets in which 25% of the control diet was substituted by cellulose, starch or pectin. Due to this substitution, dietary CP levels were lower in the cellulose (15.5% CP, % DM), pectin (15.4% CP, % DM) and starch diet (15.2% CP, % DM). Following a 15-d adaptation period, feces were collected for 5 d and ileal digesta for a total of 24 h. Starch increased SID of CP, while cellulose and pectin had no significant effect on the digestibility of CP. Overall, starch supplementation resulted in higher (p<0.05) SID values of histidine, isoleucine, threonine, alanine, aspartic acid, cysteine, glycine and serine compared with cellulose, while pectin decreased (p<0.05) SID of valine and proline compared with the starch and control diet. Both cellulose and pectin reduced (p<0.05) the ATTD of CP and AA, while starch decreased (p<0.05) ATTD of phenylalanine, alanine, proline and serine compared with the control. With regard to bacterial AA composition of the fecal mixed bacterial mass (MBM), cellulose supplementation increased (p<0.05) its content of N and almost all AA, except for valine, while pectin caused higher contents of arginine, histidine and proline compared with the control (p<0.05). The bacterial contribution of arginine in feces was higher (p<0.05) in the cellulose treatment, while pectin reduced (p<0.05) the bacterial contribution of leucine, alanine, glutamic acid and proline in feces compared with the control. In conclusion, the effects of cellulose, starch and pectin on SID were rather small. Bacterial activity in the large intestine can only explain the reduced ATTD values for arginine in the cellulose treatment, but not for the other AA in the cellulose and pectin treatments, suggesting higher endogenous losses of these AA in the large intestine.

• Asian-Australasian Journal of Animal Sciences
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• 제27권12호
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• pp.1712-1720
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• 2014

Little is known about the gender differences in energetic requirements of goats in early life. In this study, we determined the energy requirements for maintenance and gain in intact male, castrated male and female Saanen goat kids using the comparative slaughter technique and provide new data on their body composition and energy efficiency. To determine the energy requirements for maintenance, we studied 21 intact males, 15 castrated males and 18 females ($5.0{\pm}0.1kg$ initial body weight (BW) and $23{\pm}5d$ of age) using a split-plot design with the following main factors: three genders (intact males, castrated males, and females) and three dry matter intake levels (ad libitum, 75% and 50% of ad libitum intake). A slaughter group included three kids, one for each nutritional plane, of each gender, and all three animals within a group were slaughtered when the ad libitum kid reached 15 kg in BW. Net energy requirements for gain were obtained for 17 intact males, eight castrated males and 15 females ($5.1{\pm}0.4kg$ BW and $23{\pm}13d$ of age). Animals were fed ad libitum and slaughtered when they reached 5, 10, and 15 kg in BW. A digestion trial was performed with nine kids of each gender to determine digestible energy, metabolizable energy and energy metabolizability of the diet. Our results show no effect of gender on the energy requirements for maintenance and gain, and overall net energy for maintenance was $205.6kJ/kg^{0.75}$ empty body weight gain (EBW) ($170.3kJ/kg^{0.75}$ BW) from 5 to 15 kg BW. Metabolizable energy for maintenance was calculated by iteration, assuming heat production equal to metabolizable energy intake at maintenance, and the result was $294.34kJ/kg^{0.75}$ EBW and $k_m$ of 0.70. As BW increased from 5 to 15 kg for all genders, the net energy required for gain increased from 9.5 to 12.0 kJ/g EBW gain (EWG), and assuming $k_g=0.47$, metabolizable energy for gain ranged from 20.2 to 25.5 kJ/g EWG. Our results indicate that it is not necessary to formulate diets with different energetic content for intact male, castrated male and female Saanen goat kids weighing from 5 to 15 kg.