• Preventive Nutrition and Food Science
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• 제9권3호
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• pp.283-288
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• 2004

Superoxide dismutase (SOD) from tomato (Lycopersicon esculentum Mill.) fruit was purified by ammonium sulphate precipitation, Sephadex G-100 and DEAE-cellulose column chromatographies. A 22 fold purification and an overall yield of 44% were achieved. The purified enzyme was a homodimer with Mr 37.1 kDa and subunit Mr 18.2 kDa as judged by SDS-PAGE. SOD showed $K_{m}$ values of 25 ${\times}$ 10$^{-6}$ M and 1.7 ${\times}$ 10$^{-6}$ M for nitroblue tetrazolium (NBT) and riboflavin as substrates, respectively. The enzyme was thermostable upto 5$0^{\circ}C$ and exhibited pH optima of 7.8. The effect of metal ions and some other compounds on enzyme activity was studied. $Co^{2+}$ and $Mg^{2+}$ were found to enhance relative enzyme activities by 27 % and 73 %, respectively, while M $n^{2+}$ inhibited the SOD activity by 64%. However, $Ca^{2+}$ and C $u^{2+}$ had no effect on enzyme activity. Other compounds like $H_2O$$_2$ and Na $N_3$ inhibited enzymatic activities by 60% and 32%, respectively, while sodium dodecyl sulphate (SDS), chloroform plus ethanol and $\beta$-mercaptoethanol had no effect on the activity of SOD. of SOD.

• 실천공학교육논문지
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• 제16권3_spc호
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• pp.283-290
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• 2024

소프트웨어 교육은 4차 산업혁명 시대에 중요한 교과로 우리나라 초중등 교육과정에서도 점차적으로 시수를 확대하고 있으며 대학에서도 소프트웨어 관련 교과를 개설하여 전공과 상관없이 교양과목을 의무적으로 수강하고 있다. 교육부는 초중등학교에서의 프로그래밍 성취기준을 제시하였고 교육목표로 선수 과목의 연계성도 강조하고 있다. H 대학의 프로그래밍 수업 신청자도 초중등 과정에서 정보 수업을 이수하였으나 연계된 수업이 이루어지지 못하고 프로그래밍을 처음 접하는 수강자가 많았다. 이에 본 연구는 프로그래밍 수강자의 설문 데이터와 성취도 점수를 활용하여 데이터를 분석했다. 분석 결과 초중등 과정에서 이수한 정보 수업이 고등교육 과정으로 연계가 전혀 이루어지지 않았으며 문제 해결을 위한 개선점을 도출하였다. 본 연구는 고등교육과정에서 효과적인 소프트웨어 교육을 위한 연구로서 의의가 있다.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• 제5B권3호
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• pp.283-288
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• 2005

A portable primary inductance standard set that includes a Maxwell-Wien bridge and a 10 mH standard inductor installed in a thermostat has been developed at KRISS. Two auxiliary resistance capacitance networks (analogous to a 'Wagner ground') provide excellent stability of the bridge balance and impose less strict requirements on the components of these networks. Removable capacitance and ac-dc resistance standards used in the bridge arms made it possible to reproduce 10 mH and 100 mH inductance values in the frequency range of 500 Hz to 3 kHz. From investigations of this standard and preliminary comparison with VNIIM (D. I. Mendeleyev Institute for Metrology), the results have demonstrated that the bridge can be used as a part of the transportable inductance standard with a measurement uncertainty within (1-3) $\mu$H/H at frequencies of 1 kHz and 1.6 kHz. The application of the bridge as a constituent part of the transportable standard gives us an opportunity to eliminate the influence of the standard inductors.

• 한국균학회지
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• 제23권3호통권74호
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• pp.275-283
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• 1995

The nutritional requirements of mycelial growth for Pleurotus spp. were studied. As the carbon sources, soluble starch and dextrin, nitrogen sources, arginine and ammonium tartrate, and calcium sources, $CaCO_3$ enhanced mycelial growth. Optimum C/N ratio was 100 : 1. On the base of results from the experiment on nutritional requirements, the following receipe is suggested for growth of Pleurotus spp.; starch 15 g, arginine 3.484 g, ammonium tartrate 3.06 g, $CaCO_3$ 0.314 g, $KH_2PO_4$ 8.138 g, $K_2HPO_4$ 1.584 g, $MgSO_4{\cdot}7H_2O$ 1.5 g for P. sajor-caju and 2.5 g for P. ostreatus. $FeSO_4{\cdot}7H_2O$ 0.02 g, $ZnSO_4{\cdot}7H_2O$ 0.03 g, $MnSO_4{\cdot}7H_2O$ 0.02 g, optimum pH 6.0. This new synthetic medium is tentatively designated as Park's medium.

• Journal of Microbiology
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• 제44권3호
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• pp.276-283
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• 2006

The thermophilic fungus Thermoascus aurantiacus 179-5 produced large quantities of a glucosidase which preferentially hydrolyzed maltose over starch. Enzyme production was high in submerged fermentation, with a maximal activity of 30 U/ml after 336 h of fermentation. In solid-state fermentation, the activity of the enzyme was 22 U/ml at 144 h in medium containing wheat bran and 5.8 U/ml at 48 h when cassava pulp was used as the culture medium. The enzyme was specific for maltose, very slowly hydrolyzed starch, dextrins (2-7G) and the synthetic substrate (${\alpha}$-PNPG), and did not hydrolyze sucrose. These properties suggest that the enzyme is a type II ${\alpha}$-glucosidase. The optimum temperature of the enzyme was $70^{\circ}C$. In addition, the enzyme was highly thermostable (100% stability for 10 h at $60^{\circ}C$ and a half-life of 15 min at $80^{\circ}C$), and stable within a wide pH range.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 2002년도 proceedings of the second asia international conference on tribology
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• pp.283-284
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• 2002

Ball bearings (BB) are generally used in spindle of‘ disk drives at present, but they have been known that BB generate high frequency vibration. Fluid dynamic bearings (FDB) having high-rotational accuracy and small vibration characteristics have been developed as next generation spindles. Especially. a ferro fluid bearing (FFB) spindle has the advantage to prevent leakage and dispersion of lubricating oil using a ferro seal. In this study, we measured damping characteristics and frequency characteristics of these bearing spindles using a high-frequency vibration base. High frequency excitation was added to these bearing spindles mounted on the vibration base, and we proved that FFB and FDB spindles have effective damping.

• KSBB Journal
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• 제13권5호
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• pp.497-501
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• 1998

A fungus with high phosphate solubilizing activites was isolated from soil using potato dextrose agar-calcium phosphate medium and identified to Penicillium sp. PS-113, based on the morphological characteristics of conidiophore and conidia; flask shape of phialide, simple branching type of conidiophore, and columnar shape of conidial head, in malt extract agar and potato dextrose agar media. The optimum temperature ad initial pH to solubilize rock phosphate in potato dextrose broth-rock phosphate medium were 30$^{\circ}C$ and pH 8.0, respectively. In these conditions phosphate solubilizing activities of Penicillium sp. PS-113 against four types of insoluble phosphate like tricalcium-phosphate, aluminium phosphate, hydroxyapatite and rock phosphate were quantitatively determined. As results, this fungus highly produced free phosphates to the culture broth with the concentrations of 1,283 ppm against tricalcium-phosphate, 585 ppm against rock phosphate, 528 ppm against aluminium phosphate, and 242 ppm against hydroxyapatite, respectively.

• 한국산업융합학회 논문집
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• 제10권4호
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• pp.275-283
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• 2007

This study aims at the calculation of a variation of flow characteristics of main channel for tributary inflow in river junction. So this study was analyzed the variation of flow depth and velocity in main channel for a change of inflow degree. For this purpose, FLDWAV model are carried out for variations of $30^{\circ}$, $60^{\circ}$ and $90^{\circ}$ tributary inflow at junction. Results show that velocity ratio(V1/V3) increases and flow depth ratio(H1/H3) decreases for discharge ratio(Q1/Q3) of upstream and downstream when degree increases in junction. And FLDWAV model was applied at a real river junctions. Selected area is a junction of Gumho river and Sin stream. Results show that pattern is similar to a virtual channel.

• Korean Journal of Mathematics
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• 제17권3호
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• pp.283-291
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• 2009

We investigate the existence of multiple solutions (${\xi},{\eta}$) for perturbations of the parabolic system with Dirichlet boundary condition $$(0.1)\;\begin{array}{lcr}{\xi}_t=-L{\xi}+g_1(3{\xi}+{\eta})-s{\phi}_1-h_1(x,t)\;in\;{\Omega}{\times}(0,\;2{\pi}),\\{\eta}_t=-L{\eta}+g_2(3{\xi}+{\eta})-s{\phi}_1-h_2(x,t)\;in\;{\Omega}{\times}(0,\;2{\pi}).\end{array}$$ We show the existence of multiple solutions (${\xi},{\eta}$) for perturbations of the parabolic system when the nonlinearity $g^{\prime}_1,\;g^{\prime}_2$ are bounded and $3g^{\prime}_1(-{\infty})+g^{\prime}_2(-{\infty})<{\lambda}_1,\;{\lambda}_n<3g^{\prime}_1(+{\infty})+g^{\prime}_2(+{\infty})<{\lambda}_{n+1}$.

• E2M - 전기 전자와 첨단 소재
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• 제9권3호
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• pp.277-283
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• 1996

The temperature dependent characteristics of hydrogenerated amorphous silcon thin film transistor (a-Si:H TFT) with a bottom gate of N-Type <100> Si wafer were investigated. Drain current on the hysteresis characteristic curve showed an exponential variation. Hysteresis area of TFT increased with the gate voltage increased and decreased with the small gate voltage. According to the variation of gate voltages, drain current of TFT increased by temperature increase, and hysteresis characteristics mainly depended on the temperature increase. The hysteresis current showed negative characteristics curve over 383K. The hysteresis occurance area and the differences of forward and reverse sweep were increased at the higher temperature. Hysteresis current of I$_{d}$(on/off) ratio decreased at the lower temperature and increased at the higher temperature.e.