Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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A Specific Short Dextrin-Hydrolyzing Extracellular Glucosidase from the Thermophilic Fungus Themoascus aurantiacus 179-5

  • Carvalho Ana Flavia Azevedo (Laboratory of Biochemistry and Applied Microbiology, IBILCE, Sao Paulo State University) ;
  • Goncalves Aline Zorzetto (Laboratory of Biochemistry and Applied Microbiology, IBILCE, Sao Paulo State University) ;
  • Silva Roberto da (Laboratory of Biochemistry and Applied Microbiology, IBILCE, Sao Paulo State University) ;
  • Gomes Eleni (Laboratory of Biochemistry and Applied Microbiology, IBILCE, Sao Paulo State University)
  • Published : 2006.06.01
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Abstract

The thermophilic fungus Thermoascus aurantiacus 179-5 produced large quantities of a glucosidase which preferentially hydrolyzed maltose over starch. Enzyme production was high in submerged fermentation, with a maximal activity of 30 U/ml after 336 h of fermentation. In solid-state fermentation, the activity of the enzyme was 22 U/ml at 144 h in medium containing wheat bran and 5.8 U/ml at 48 h when cassava pulp was used as the culture medium. The enzyme was specific for maltose, very slowly hydrolyzed starch, dextrins (2-7G) and the synthetic substrate (${\alpha}$-PNPG), and did not hydrolyze sucrose. These properties suggest that the enzyme is a type II ${\alpha}$-glucosidase. The optimum temperature of the enzyme was $70^{\circ}C$. In addition, the enzyme was highly thermostable (100% stability for 10 h at $60^{\circ}C$ and a half-life of 15 min at $80^{\circ}C$), and stable within a wide pH range.

Keywords

References

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