• Journal of Nutrition and Health
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• v.41 no.3
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• pp.224-231
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• 2008

We examined the effect of indole-3-carbinol (I3C, $C_9H_9NO$), an autolysis product of a glucosinolate and a glucobrassicin in vegetables, on MMP-2, -9 activities and TIMP-l and -2 inductions via microtubule-associated protein kinase (MAPK) signaling pathway in prostate cancer cell line, PC3 cells. Our results indicated that I3C inhibited cell growth of PC3 cells in dose (0,50, 100 ,${\mu}M$) and time (0,24,48 and 72 h) dependent manners. Using gelatin zymography for MMP activity, we demonstrated that I3C significantly decrease MMP-2 and -9 activities in PC3 cells. We also observed that I3C decreased the proteins and mRNA levels of MMP-2 and -9 in PC3 cells as well. Inversely, expressions of TIMP-l and -2 protein and mRNA in PC3 cells were increased by I3C in a dose dependent manner. In another experiment, we showed that I3C inhibited PC3 cells invasiveness by using marigel invasion assay and we also found that I3C suppressed MMP transcriptional activity by MAPK signaling pathways. Taken together, our results suggest that I3C may contribute to the potential beneficial food component to prevent the cancer metastasis in prostate cancer cells. (KoreanJNutr2008; 41(3): 224~23I)

• Korean Journal of Environmental Agriculture
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• v.36 no.4
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• pp.293-298
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• 2017

BACKGROUND: Pyrethroids (PYRs) are a widely used insecticide in agriculture and household area. In mammals, PYRs such as deltamethrin is metabolized to 3-phenoxybenzoic acid (3-PBA) in liver that is mainly excreted in urine. This study is designed to single exposure of deltamethrin to rats in a dose-dependent manner and identify the correlation between deltamethrin exposure and its metabolite (3-PBA) in urine. METHODS AND RESULTS: Exposure levels of deltamethrin were control (0 mg/kg bw), low (0.0705 mg/kg bw), medium (0.705 mg/kg bw) and high (7.05 mg/kg bw) dose. Low concentration was derived by ussing Korea predictive operator exposure model (KoPOEM). Dermal exposure persisted for 6 h, and urine specimens were collected for 24 h. The urine matrix was removed after a series of procedures and 3-PBA was analyzed by gas chromatography/mass spectrometry. CONCLUSION: There was a strong correlation ($R^2=0.83$) between the amount of oral exposure to delta me thrin and urinary levelof3-PBAexcreted. In dermal exposure groups of deltamethrin except high-dose, also there was a good correlation between urinary 3-PBA and deltamethrin exposure, but not stronger than in oral deltamethrin exposure groups. Based on these results, therefore, the amount of 3-PBA in urine can be used as a good monitoring indicator that reflexing the exposure level of deltamethrin to human body.

• Applied Microscopy
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• v.26 no.4
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• pp.389-399
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• 1996

In order to understand the morphological differences between two different organic loadings by its upstream, and to compare with other algal groups with references, the fine structure of blue-green algae, Microcystis aeruginosa Kitzing, taken from two branches, Tongbok and Bosung stream of Lake Chuam, Korea pennisula was examined. It showed extinct differences in most physicochemical factors between both branches, except water temperature and pH values. The concentrations of total phosphorus in Tongbok branch were twice as those of Bosung. M. aeruginosa cells were enumerated totally $1.2X10^4cells/ml$ and these individuals in branch of Tongbok were close to two times as much as Bosung. In light and electron microscopy, natural M. aeruginosa colonies formed irregular shape and non-directional array in amorphous matrix. They were consisted of many kinds of cells, youngs or olds in cell division, solitary, and various size of cells. Each cell ranged from 2.61 to $5.40{\mu}m$ in diameter, and averaged as $3.54{\pm}0.19{\mu}m$. In cytoplasm, they contained a number of inclusions in various size, shape and appearances. Among them, polyhedral bodies or carboxysomes, a structured granules, photosynthetic lamellae or thylakoids, and gas vacuoles were prominent and easy to recognize. Although it was failed to find the definable morphological variations in the ultrastructure of M. aeruginosa in terms of algal habitual environments, some useful characters were founded, outer layer of cell wall, polyhedral bodies and gas vacuoles, in blue-green algal classification and taxonomy.

• Applied Microscopy
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• v.34 no.1
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• pp.1-11
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• 2004

The wound healing is very complex biological processing including inflammatory, reepithelialization and matrix construction. According to the biological systematic category, the ability of the healing is very different. Generally healing ability of the lower animal group has been known more excellent compared to its higher group. Therefore, lower animals have been used as the experimental model to explore the mechanism of the wound healing or repair. To verify histochemical characteristics of the wound healing, we have used skin of the frog (Bombina orientalis) as known common amphibian. At day 1, 10, and 16, the mucous substance was very actively synthesized and strong positive by PAS and Alcian blue (pH 2.5). Day 10 after wounding, margin of the wound was gradually strong positive by PTAH staining for detection of collagen synthesis. At 3 to 6 hour and day 23 to 27, we have found the cell division was active through the MG-P staining, in which the concentration and division of DNA in nucleus was green to deep blue color.

• The Journal of the Petrological Society of Korea
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• v.23 no.4
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• pp.311-324
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• 2014

The Hongjeom formation of the Pyeongan Supergroup in the Munkyeong coalfield mainly consists of metapsammite and metapelites. Metampelites occur as slate preserving chloritoid+chlorite+muscovite and andalusite+biotite+chlorite+muscovite mineral assemblages. Chloritoid and andalusite occur as porphyroblast, and the matrix composed of fine-grained micas. Metamorphic P-T conditions for these mineral assemblages are $510-520^{\circ}C$ and 3.0-3.5kbar based on P-T pseudosection in $MnO-K_2O-FeO-MgO-Al_2O_3-SiO_2-H_2O(MnKFMASH)$ system and isopleth intersections of Fe/(Fe+Mg) ratios in chloritoid and chlorite. The medium temperature and low pressure metamorphism resulted from a higher geothermal gradient ($40-45^{\circ}C/km$) condition than that of burial metamorphism. The youngest (SHRIMP U-Pb age; ca. 327-310 Ma) detrital zircon grains from the Hongjeom formation display oscillatory zoning and relatively high Th/U ratio (0.60-1.12). Based on the previous sedimentary, paleontological, and geochronological studies in the Taebaeksan basin together with results of this study, we suggest that (1) initial deposition of the Hongjeom formation was contemporaneous with a magmatic activity in the provenance, (2) the Pyeongan Supergroup was deposited in an arc-related basin at an active continental margin during the Carboniferous to Permain, and (3) magmatic activities occurred repetitively in relatively short interval in the active continental margin had continuously supplied sediments to the basin.

• Food Science and Preservation
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• v.24 no.5
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• pp.688-696
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• 2017

In this study, the physicochemical properties and anti-wrinkle effect of polysaccharides with different molecular weights from Gloiopeltis furcata were investigated. Crude polysaccharides were isolated by viscozyme treatment followed by ethanol precipitation and lyophilization. Crude polysaccharides were hydrolyzed by acid (0.1 N HCl) and the molecular weight fractions were generated by centrifugal filter (<10 kDa, 10 to 100 kDa, and 100 kDa>). The yield of polysaccharides with different molecular weight fractions was 8.4-39.6%. The major constituents in molecular weight fractions were total sugar (81.37-85.82%), uronic acid (27.89-32.85 g/100 g), sulfate (33.38-39.04%), and protein (0.35-3.16%) The L, a, and b value of the 100 kDa group were decreased, but viscosity increased. The oxygen radical absorbance capacity of the 100 kDa group at $180.07{\mu}M$ was the highest among groups. The protective effects of 100 kDa group at 0.5 and $5{\mu}g/mL$ against $H_2O_2$-induced cytotoxicity in L132 cell were 87.34% and 103.85%, respectively. The matrix metalloproteinase-1 activity of 100 kDa group decreased in a dose-dependent manner. The pro-collagen synthesis activity of 100 kDa group at $0.05-0.5{\mu}g/mL$ was 64.91-77.80%. The polysaccharides with different molecular weights from Gloiopeltis furcata investigated herein are useful as a potential candidate for cosmedical materials.

• Korean Journal of Crystallography
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• v.2 no.1
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• pp.17-22
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• 1991

The crystal structure of an acetylene sorption complex of vacuum dehydrated fully Cda+ _exchanged zeolite A has been determined from three-dimensional X-ray diffraction data gathered by counter method. The structure was solved and refined in the cubic space group Pm3m at 294(1) K, a=12.202(3) A and Z=1. We crystal was prepared by dehydration at 723 K and 2.67×104 Pa for 2 days, followed by exposure to 1.60×104 Pa of acetylene gas at 298(1) K. All six Cd2+ions per unit cell are associated with 6-oxgen rings of the aluminosilicate framework. They are distributed over two distinguished threefold axes of unit cell; two of these Cd2+ ions are recessed 0.694 into the sodalite unit from (111) plane of three 0(3)'s and each approaches three framework oxides; the other four Cd2+ ions extend approximately 0.586A into the large cavity. The four Cd2+ ions are in a near tetrahedral environment, 2.220(9)A from·three framework oxide ions and 2.74(7) A from each carbon atom of an acetylene molecule(which is here counted as a monodentate ligand). Full matrix least squares refinement converged to the final error indices R1=0.093 and R2=0.105 using the 292 independent reflections for which I>3σ(I).

• Journal of Ginseng Research
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• v.36 no.2
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• pp.161-168
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• 2012

The abnormal maturation and ossification of articular chondrocytes play a central role in the pathogenesis of osteoarthritis (OA). Inhibiting the enzymatic degradation of the extracellular matrix and maintaining the cellular phenotype are two of the major goals of interest in managing OA. Ginseng is frequently taken orally, as a crude substance, as a traditional medicine in Asian countries. Ginsenoside $Rb_1$, a major component of ginseng that contains an aglycone with a dammarane skeleton, has been reported to exhibit various biological activities, including anti-inflammatory and anti-tumor effects. However, a chondroprotective effect of ginsenoside $Rb_1$ related to OA has not yet been reported. The purpose of this study was to demonstrate the chondroprotective effect of ginsenoside $Rb_1$ on the regulation of pro-inflammatory factors and chondrogenic genes. Cultured rat articular chondrocytes were treated with 100 ${\mu}M$ ginsenoside $Rb_1$ and/or 500 ${\mu}M$ hydrogen peroxide ($H_2O_2$) and assessed for viability, reactive oxygen species production, nitric oxide (NO) release, and chondrogenic gene expression. Ginsenoside $Rb_1$ treatment resulted in reductions in the levels of pro-inflammatory cytokine and NO in $H_2O_2$-treated chondrocytes. The expression levels of chondrogenic genes, such as type II collagen and SOX9, were increased in the presence of ginsenoside $Rb_1$, whereas the expression levels of inflammatory genes related to chondrocytes, such as MMP1 and MMP13, were reduced by approximately 50%. These results suggest that ginsenoside $Rb_1$ has potential for use as a therapeutic agent in OA patients.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.39 no.5
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• pp.11-20
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• 2002

Based on regularization principles, we propose a new dequantization scheme on DCT-based transform coding for reducing of blocking artifacts and minimizing the quantization error. The conventional image dequantization is simply to multiply the received quantized DCT coefficients by the quantization matrix. Therefore, for each DCT coefficients, we premise that the quantization noise is as large as half quantizer step size (in DCT domain). Our approach is based on basic constraint that quantization error is bounded to ${\pm}$(quantizer spacing/2) and at least there are not high frequency components corresponding to discontinuities across block boundaries of the images. Through regularization, our proposed dequantization scheme, sharply reduces blocking artifacts in decoded images. Our proposed algorithm guarantees that the dequantization process will map the quantized DCT coefficients will be evaluated against the standard JPEG, MPEG-1 and H.263 (with Annex J deblocking filter) decoding process. The experimental results will show visual improvements as well as numerical improvements in terms of the peak-signal-to-noise ratio (PSNR) and the blockiness measure (BM) to be defined.

• Journal of the Korean Institute of Telematics and Electronics D
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• v.36D no.8
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• pp.36-44
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• 1999

This paper presents the design and fabrication of the duplexer for a Ka-band satellite transponder which is analyzed transmission characteristics by calculating the generalized scattering matrix using mode matching method. It is composed of 2 bandpass filters, coupled with H-plane T-junction having symmetrical inductive iris and E-plane metal insert structures. Compared with the size and weight of the Rx and Tx filter loaded with a transponders respectively, those of the duplexer can be effectively reduced. In a high power transmission, the variation of the filter characteristics is minimized by the scheme that irises are extended to the exterior of H-plane of the waveguide. This scheme needs no extra heat sinks for dissipating high power. The duplexer is designed to improve the simplification, durability and reliability by eliminating tuning screws, which have been used to compensate for the characteristics of fabricated filters. The bandpass filters of the duplexer show the insertion loss of less than 1.2 dB and the return loss in excess of 15 dB. The isolations of more than 65 dB have been achieved between Rx and Tx filter.