Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Histochemical Analysis of the Cutaneous Wound Healing in the Amphibian

양서류 피부 상처회복과정에 대한 조직화학적 분석

  • Lim, Do-Sun (Department of Dental Hygiene, Seoul Health College) ;
  • Jeong, Soon-Jeong (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Jeong, Je-O (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Park, Joo-Cheol (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Kim, Heung-Joong (Department of Oral Anatomy, College of Dentistry, Chosun University) ;
  • Moon, Myung-Jin (Department of Biological Science, Dankook Univeristy) ;
  • Jeong, Moon-Jin (Department of Oral Histology, College of Dentistry, Chosun University)
  • 임도선 (서울보건대학 치위생과) ;
  • 정순정 (조선대학교 치과대학 구강조직학교실) ;
  • 정제오 (조선대학교 치과대학 구강조직학교실) ;
  • 박주철 (조선대학교 치과대학 구강조직학교실) ;
  • 김흥중 (조선대학교 치과대학 구강해부학교실) ;
  • 문명진 (단국대학교 첨단과학부 생물학과) ;
  • 정문진 (조선대학교 치과대학 구강조직학교실)
  • Published : 2004.03.01
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Abstract

The wound healing is very complex biological processing including inflammatory, reepithelialization and matrix construction. According to the biological systematic category, the ability of the healing is very different. Generally healing ability of the lower animal group has been known more excellent compared to its higher group. Therefore, lower animals have been used as the experimental model to explore the mechanism of the wound healing or repair. To verify histochemical characteristics of the wound healing, we have used skin of the frog (Bombina orientalis) as known common amphibian. At day 1, 10, and 16, the mucous substance was very actively synthesized and strong positive by PAS and Alcian blue (pH 2.5). Day 10 after wounding, margin of the wound was gradually strong positive by PTAH staining for detection of collagen synthesis. At 3 to 6 hour and day 23 to 27, we have found the cell division was active through the MG-P staining, in which the concentration and division of DNA in nucleus was green to deep blue color.

상처회복과정은 염증, 재상피화, 그리고 기질의 재형성등을 포함한 포괄적인 생물학적 반응이다. 생물학적 분류체계에서, 회복능력은 매우 다양한데, 일반적으로 하등동물이 고등동물에 비교해 단순하며 뛰어난 것으로 알려져 있다. 따라서, 하등동물들을 이용한 실험모델은 상처회복과 치유의 기작을 연구하는데 자주 이용되고 있다. 양서류를 이용한 피부 상처회복과정 동안의 조직화학적 특성들을 규명하기 위하여 한국산 무당개구리(Bombina orientalis)를 이용하였다. 1일, 10일 그리고 16일의 조직에서 점액물질이 활발하게 분비되는 것을 확인하였으며, PAS (periodic acid shiff)와 Alcian blue (pH 2.5) 반응에 강하게 염색되었다. PTAH(phosphotungstic acid hematoxylin) 염색을 통하여 상처 후 10일 조직에서 상처주변의 진피층에서 콜라겐의 합성이 증가하는 것을 확인하였다. 또한, 세포의 유사분열의 시점을 확인하기 위한 MG-P (methyl green pyronin)염색에서 초기에는 3시간과 6시간에 강한 염색반응을 관찰하였고, 이는 23일과 27일 조직에서도 동일한 패턴으로 이어졌다.

Keywords

References

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