• 제목/요약/키워드: H-continuous

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Zymomonas mobilis에 의한 Packed Bed Reactor를 이용한 연속적인 sorbitol의 형성 (Continuous Production of Sorbitol with Zymomonas mobilis in a Packed Bed Reactor)

  • 장기효;김영복장현수전억한
    • KSBB Journal
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    • 제11권1호
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    • pp.58-64
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    • 1996
  • K-carrageenan에 고정화시킨 Zymomonas mobilis를 이용한 연속적인 sorbitol 생산에 대하여 연구하였다. Toluene과 glutaraldehyde로 처리하여 투과성을 증대시킨 세포를 alginate나 chitin 고정화 공정에서 어느 정도의 효소활성을 보였으나 210시간 이상의 공정반응에서는 sorbitol 생성량이 감소되었다. 독성이 적고 투과성을 증가시키는 물질로서 toluene 대신 CTAB(Cetyltrimethylammoniumbromide)를 사용하였다. CTAB로 투과성을 증가시킨 세포를 k-carrageenan에 고정화하여 CSTR과 packed bed reactor를 이용하여 sorbitol 생산을 시도하였으며 CSTR보다도 (25일) packed bed reactor에서 (30일) 더 긴 시간 효소활성도가 유지되었다. Two-stage 연속공정에서는 희석비율이 (dilution rate, $h^{-1}$) 증가함에 따라서 sorbitol 생성률이 증가되었으며 희석비율 0.32 $h^{-1}$에서 첫번째와 두번째 반응조에서 각각 $15g/\ell-h,$ $22g/\ell-h의$ sorbitol 생성률을 얻었다. 0.32$h^{-1}$보다 높은 희석비율에서는 생성를이 감소되었다.

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차동 복조 2-h 연속 위성 변조의 오류 확률 (The Error Rate Evaluation for Differential Demodulation of 2-h Continuous Phase Modulation)

  • 윤동원;한영열
    • 전자공학회논문지A
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    • 제31A권7호
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    • pp.29-35
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    • 1994
  • The performance of continuous phase modulation signals is well known for the coherent demodulation. But the carrier recovery circuits of the coherent receiver have long acquisition time and the receiver experiences high error floors for fading channels. In this paper, we propose the differential demodulation of 2-h continuous phase modulation signals. The sets of modulation indices of 2-h phase codes adequate to the differential demodulation for differentially encoded input are obtained and the average bit error probability in Additive White Gaussian Noise environments is derived and analyzed.

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Growth, carcass traits, immunity and oxidative status of broilers exposed to continuous or intermittent lighting programs

  • Ghanima, Mahmoud M. Abo;El-Hack, Mohamed E. Abd;Abougabal, Mohammed Sh.;Taha, Ayman E.;Tufarelli, Vincenzo;Laudadio, Vito;Naiel, Mohammed A.E.
    • Animal Bioscience
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    • 제34권7호
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    • pp.1243-1252
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    • 2021
  • Objective: An experiment was conducted to investigate the continuous and intermittent lighting program effects on terms of the productive performance, carcass traits, blood biochemical parameters, innate immune and oxidative status in broiler chicks. Methods: A total of 600 Cobb-500 one day old chicks were randomly allocated into six equal groups (100 chicks per treated group with five replicates of 20 chicks each) based on lighting program; 22 continuous lighting (22 C), 11 h lighting+1 darkness twice daily (11 L/1 D), 20 h continuous lighting (20 C), 5 h lighting+1 darkness four times daily (5 L/1 D), 18 h continuous lighting (18 C) and the final group subjected for 3 h lighting+1 h darkness six times daily (3 L/1 D). The experimental period lasted 42 days. Results: Compared with those under the intermittent light program, broiler chicks exposed to continuous lighting for 22 h had significant improvement in live body weight and carcass (dressing and breast percentage) measured traits. Though reducing lighting hours significantly reduced feed intake and feed conversion ratio values. Different lighting programs revealed no significant effect on all blood biochemical parameters. Oxidative stress and innate immunity parameters significantly enhance by reducing lighting hours (3L/1D). Conclusion: The findings suggest that reducing lighting hours up to 3L/1D would be more useful in enhancing feed efficiency, innate immunity, and oxidative status compared with continuous lighting programs on broilers.

투과화된 Zymomonas mobilis 균체를 이용한 Glucose와 Fructose로 부터 Gluconic Acid와 Sorbitol의 생산 (Continuous Production of Gluconic Acid and Sorbitol from Glucose and Fructose using Perrneabilized cells of Zymomonas mobilis)

  • 김원준;박제균;김학성
    • 한국미생물·생명공학회지
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    • 제19권1호
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    • pp.70-75
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    • 1991
  • Continuous and simultaneous production of gluconic acid and sorbitol from glucose and fructose was carried out by using glucose-fructose oxidoreductase and glucanolactonase of Zymomonas mobilis. In order to utilize the enzymes without purification, Zymomonas mobilis was permeabilized with toluene. Optimum conditions for permeabilization and reaction kinetics of permeabilized Zymomonas mobilis were studied. In batch operation with the permeabilized cells immobilized in alginate beads, about 90% conversion was obtained within 35 h reaction. Continuous production of gluconic acid and sorbitol using the immobilized permeabilized cells was carried out. Optimum conditions for continuous operation with the imn~obilized cells were; pH 6.2 and temperature $40^{\circ}C$. Maximum productivities for gluconic acid and sorbitol were about 14.5 g/l/h and 14.8 g/l/h respectively at the dilution rate of 0.075 $h^{-1}$ when 300 g/l each of substrates was fed.

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고정화 효모를 이용한 연속적 에탄올 생산 (Continuous Ethanol Production Using immobilized Baker's Yeast)

  • 한면수;하상도;정동효
    • 한국미생물·생명공학회지
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    • 제19권4호
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    • pp.398-404
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    • 1991
  • 효모를 Na-alginate에 고정화한 후 연속반응기를 이용한 glucose 발효로 에탄올을 생산하였다. 그 결과 고정화 효모의 활성화 시간은 20~25시간이었다. 연속발효에서 고정화효모의 온도안정성은 30~$37^{\circ}C$였으며 pH 안정성은 pH 4.0~pH 8.0, 최적 희석속도는 $0.2h^[-1}$ 이었고 에탄올생산 최적 당농도는 15%였다. 최적조건에서 에탄올수율은 0.23, 생산된 에탄올 농도는 33.90g/l 그리고 에탄올 생산성은 7.12g/$l\cdot h$로 각각 나타났다.

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Production of Lactococcal Bacteriocin using Repeated-Batch and Continuous Cultures

  • Yoo, Jin-Young
    • Journal of Microbiology and Biotechnology
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    • 제2권4호
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    • pp.284-287
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    • 1992
  • Repeated-batch and continuous cultures of Lactococcus sp. 1112-1 were carried out for bacteriocin production using a glucose-casein medium. Repeated-batch culture did not efficiently enhanced the bacteriocin production. Continuous production was possible at the dilution rate of 0.4 $h^{-1}$. Maximum specific production rate ($Q^p$), bacteriocin production and biomass at the dilution rate were 347, 136 IU/g/h, 2, 121 IU/ml and 2.45 g/L, respectively.

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저출력 레이져 자극이 근육세포의 증식 및 유전자 발현에 미치는 효과 (Effect of Low-Energy Laser Irradiation on the Proliferation and Gene Expression of Myoblast Cells)

  • 곽지현;전옥희;강동연;유현희;김경환;정병조;김지현
    • 대한의용생체공학회:의공학회지
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    • 제31권1호
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    • pp.81-86
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    • 2010
  • Laser irradiation is known to affect various tissues such as skin, bone, nerve, and skeletal muscle. Laser irradiation promotes ATP synthesis, facilitates wound healing, and stimulates cell proliferation and angiogenesis. In skeletal muscle, laser irradiation is related to the proliferation of skeletal muscle satellite cells. Normal skeletal muscle contains remodeling capacity from myogenic cells that are derived from mononuclear satellite cells. Their processes are activated by the expression of genes related with myogenesis such as muscle-specific transcription factors (MyoD and Myf5) and VEGF (vascular endothelial growth factor). In this study, we hypothesized that laser irradiation would enhance and regulate muscle cell proliferation and regeneration through modulation of the gene expressions related with the differentiation of skeletal muscle satellite cells. $C_2C_{12}$ myoblastic cells were exposed to continuous/non-continuous laser irradiation (660nm/808nm) for 10 minutes daily for either 1 day or 5 days. After laser irradiation, cell proliferation and gene expression (MyoD, Myf5, VEGF) were quantified. Continuous 660nm laser irradiation significantly increased cell proliferation and gene expression compared to control, continuous 808nm laser irradiation, and non-continuous 660nm laser irradiation groups. These results indicate that continuous 660nm laser irradiation can be applied to the treatment and regeneration of skeletal muscle tissue.

ROBUST $H_{\infty}$ FIR SAMPLED-DATA FILTERING

  • Ryu, Hee-Seob;Yoo, Kyung-Sang;Kwon, Oh-Kyu
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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    • pp.521-521
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    • 2000
  • This paper investigates the problem of robust H$_{\infty}$ filter with FIR(Finite Impulse Response) structure for linear continuous time-varying systems with sampled-data measurements. It is assumed that the system is subject to real time-varying uncertainty which is represented by the state-space model having parameter uncertainty. The robust H$_{\infty}$ FIR filter is proposed for the continuous-time linear parameter uncertain systems. It is also derived from the equivalence relationship between the robust linear H$_{\infty}$ FIR filter and the robust linear H$_{\infty}$ filter with sampled-data measurements.

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연속생물반응기에서 perchlorate 환원 세균에 의한 perchlorate의 제거 (Perchlorate Removal by Perchlorate Reducing Bacteria Consortium in a Continuous Bioreactor)

  • 류희욱
    • KSBB Journal
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    • 제27권1호
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    • pp.28-32
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    • 2012
  • In this study, the treatment ability of the wastewater containing perchlorate by non-salt tolerant perchlorate reducing bacterial consortium (N-PRBC) was evaluated in a continuous stirred tank bioreactor (CSTR). To obtain the optimal operating condition the bioreactor was operated with the different wastewater empty bed retention time (EBRT). The treatment performance in the bioreactor could be maintained at 100 $mg-ClO_4{^-}L^{-1}$ up to a EBRT of 3 h, and the removal capacity in the CSTR was about 3.3 times higher than that in a batch operation. With a decrease from 9 h to 2 h in a EBRT, the volumetric perchlorate reduction rate was increased from 11.1 $mg-ClO_4{^-}L^{-1}h^{-1}$ to 50.0 $mg-ClO_4{^-}L^{-1}h^{-1}$, and the specific perchlorate reduction rates were increased from 3.01 $mg-ClO_4{^-}g-DCW^{-1}h^{-1}$. In conclusion, the treatment capacities in a CSTR were much better than those obtained in a batch operation.