Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Low-Energy Laser Irradiation on the Proliferation and Gene Expression of Myoblast Cells

저출력 레이져 자극이 근육세포의 증식 및 유전자 발현에 미치는 효과

  • Kwag, J.H. (Department of Biomedical Engineering, Yonsei University) ;
  • Jeon, O.H. (Department of Biomedical Engineering, Yonsei University) ;
  • Kang, D.Y. (Department of Biomedical Engineering, Yonsei University) ;
  • Ryu, H.H. (Constitutional Biology and Medical Engineering Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, K.H. (Department of Biomedical Engineering, Yonsei University) ;
  • Jung, B.J. (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, C.H. (Department of Biomedical Engineering, Yonsei University)
  • 곽지현 (연세대학교 보건과학대학 의공학과) ;
  • 전옥희 (연세대학교 보건과학대학 의공학과) ;
  • 강동연 (연세대학교 보건과학대학 의공학과) ;
  • 유현희 (한국 한의학 연구원 체질 생물학 의공학 연구센터) ;
  • 김경환 (연세대학교 보건과학대학 의공학과) ;
  • 정병조 (연세대학교 보건과학대학 의공학과) ;
  • 김지현 (연세대학교 보건과학대학 의공학과)
  • Received : 2009.11.04
  • Accepted : 2010.01.27
  • Published : 2010.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Laser irradiation is known to affect various tissues such as skin, bone, nerve, and skeletal muscle. Laser irradiation promotes ATP synthesis, facilitates wound healing, and stimulates cell proliferation and angiogenesis. In skeletal muscle, laser irradiation is related to the proliferation of skeletal muscle satellite cells. Normal skeletal muscle contains remodeling capacity from myogenic cells that are derived from mononuclear satellite cells. Their processes are activated by the expression of genes related with myogenesis such as muscle-specific transcription factors (MyoD and Myf5) and VEGF (vascular endothelial growth factor). In this study, we hypothesized that laser irradiation would enhance and regulate muscle cell proliferation and regeneration through modulation of the gene expressions related with the differentiation of skeletal muscle satellite cells. $C_2C_{12}$ myoblastic cells were exposed to continuous/non-continuous laser irradiation (660nm/808nm) for 10 minutes daily for either 1 day or 5 days. After laser irradiation, cell proliferation and gene expression (MyoD, Myf5, VEGF) were quantified. Continuous 660nm laser irradiation significantly increased cell proliferation and gene expression compared to control, continuous 808nm laser irradiation, and non-continuous 660nm laser irradiation groups. These results indicate that continuous 660nm laser irradiation can be applied to the treatment and regeneration of skeletal muscle tissue.

Keywords

References

  1. Mauro A. Satellite cell of skeletal muscle fibers. J Biophys Biochem Cytol 1961 Feb;9:493-5. https://doi.org/10.1083/jcb.9.2.493
  2. Peng H, Huard J. Muscle-derived stem cells for musculoskeletal tissue regeneration and repair. Transpl Immunol 2004 Apr;12(3-4):311-9. https://doi.org/10.1016/j.trim.2003.12.009
  3. Holterman CE, Rudnicki MA. Molecular regulation of satellite cell function. Semin Cell Dev Biol 2005 Aug-Oct;16(4-5):575-84. https://doi.org/10.1016/j.semcdb.2005.07.004
  4. Martin P, Hopkinson-Woolley J, McCluskey J. Growth factors and cutaneous wound repair. Prog Growth Factor Res 1992;4(1):25-44. https://doi.org/10.1016/0955-2235(92)90003-Z
  5. Nemeth GG, Bolander ME, Martin GR. Growth factors and their role in wound and fracture healing. Prog Clin Biol Res 1988;266:1-17.
  6. Conlan MJ, Rapley JW, Cobb CM. Biostimulation of wound healing by low-energy laser irradiation. A review. J Clin Periodontol 1996 May;23(5):492-6. https://doi.org/10.1111/j.1600-051X.1996.tb00580.x
  7. Yaakobi T, Maltz L, Oron U. Promotion of bone repair in the cortical bone of the tibia in rats by low energy laser (He-Ne) irradiation. Calcif Tissue Int 1996 Oct;59(4):297-300. https://doi.org/10.1007/s002239900126
  8. Assia E, Rosner M, Belkin M, Solomon A, Schwartz M. Temporal parameters of low energy laser irradiation for optimal delay of post-traumatic degeneration of rat optic nerve. Brain Res 1989 Jan 9;476(2):205-12. https://doi.org/10.1016/0006-8993(89)91240-7
  9. Bibikova A, Oron U. Promotion of muscle regeneration in the toad (Bufo viridis) gastrocnemius muscle by low-energy laser irradiation. Anat Rec 1993 Mar;235(3):374-80. https://doi.org/10.1002/ar.1092350306
  10. Karu T. Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B 1999 Mar;49(1):1-17. https://doi.org/10.1016/S1011-1344(98)00219-X
  11. Morimoto Y, Arai T, Kikuchi M, Nakajima S, Nakamura H. Effect of low-intensity argon laser irradiation on mitochondrial respiration. Lasers Surg Med 1994;15(2):191-9. https://doi.org/10.1002/lsm.1900150207
  12. Yu W, Naim JO, McGowan M, Ippolito K, Lanzafame RJ. Photomodulation of oxidative metabolism and electron chain enzymes in rat liver mitochondria. Photochem Photobiol 1997 Dec;66(6):866-71. https://doi.org/10.1111/j.1751-1097.1997.tb03239.x
  13. Weiss N, Oron U. Enhancement of muscle regeneration in the rat gastrocnemius muscle by low energy laser irradiation. Anat Embryol (Berl) 1992 Oct;186(5):497-503.