• Title/Summary/Keyword: H-NS

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Selecting an Effective Sound for Inducing Sows and Their Piglets to Nurse and Suckle (돼지의 수.포유행동 유발에 효과적인 소리의 선정)

  • Jeon, J.H.;Yeon, S.C.;Chang, H.H.
    • Journal of Animal Science and Technology
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    • v.45 no.4
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    • pp.627-632
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    • 2003
  • Piglets communicate with their sow by using special sounds and synchronize their suckling behavior with their mother’s grunting. This study sought to find an effective sound for controlling the nursing/suckling behavior of pigs. Eighteen crossbred Landrace${\times}$Yorkshire sows and their piglets were studied on days 1, 7, and 14 post-partum. The parity of the sows was three or four. The litter size ranged from 9 to 11, with a mean of 10.4 piglets. Thirty minutes after the end of the last suckling, either the nursing-suckling sound (NSS) or the nursing-suckling sound + click sound (NSSCS) was played for 90 s randomly, and this experiment was repeated three times. The behavior of the sows and their piglets was recorded using camcorders and observed each second. When comparing NSS and NSSCS, there was less lying and sleeping and more sitting, standing, walking, and massaging when NSSCS was played than when NSS was played. These results suggest that NSSCS may induce more nursing and suckling in pigs than NSS.

Transcriptional Analysis Responding to Propanol Stress in Escherichia coli (대장균에서 프로판올 스트레스에 관한 전사분석)

  • Park, Hye-Jin;Lee, Jin-Ho
    • Journal of Life Science
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    • v.22 no.3
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    • pp.417-427
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    • 2012
  • We compared the transcriptome in response to propanol stress in wild-type and propanol-resistant mutant Escherichia coli using the DNA microarray technique. The correlation value of RNA expression between the propanol-treated wild type and the untreated-one was about 0.949, and 50 genes were differentially expressed by more than twofold in both samples. The correlation value of RNA expression between the propanol-treated mutant and the untreated one was about 0.951, and 71 genes in two samples showed differential expression patterns. However, the values between the wild type and mutant, regardless of propanol addition, were 0.974-0.992 and only 1-2 genes were differentially expressed in the two strains. The representative characteristics among differentially expressed genes in W3110 or P19 treated with propanol compared to untreated samples were up-regulation of hest shock response genes and down-regulation of genes relating to ribosome biosynthesis. In addition, many genes were regulated by transcription regulation factors such as ArcA, CRP, FNR, H-NS, GatR, or PurR and overexpressed by sigma factor RpoH. We confirmed that RpoH mediated an important host defense function in propanol stress in E. coli W3110 and P19 by comparison of cell growth rate among the wild type, rpoH disruptant mutant, and rpoH-complemented strain.

Study on Heating Load Characteristics and Thermal Curtain Effects for Simple Silkworm Rearing Houses(I) -Heating Load Coefficient and Maximum Heating Load- (간이잠실(簡易蠶室)의 난방(暖房) 부하특성(負荷特性) 및 보온(保溫)커튼 설치효과(設置効果)에 관(關)한 연구(硏究)(I) -간이잠실(簡易蠶室)의 난방(暖房) 부하계수(負荷係數) 및 최대(最大) 난방부하(暖房負荷)-)

  • Choe, K.J.;Lee, D.H.;Park, K.K.
    • Journal of Biosystems Engineering
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    • v.15 no.4
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    • pp.346-354
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    • 1990
  • In order to provide basic references for the design of heating on simple silkworm rearing house, the actual change of heating load coefficient by progress of adult silkworm rearing day from the reared in silkworm rearing house, the heating load coefficient by types of silkworm rearing houses and the heating requirement and the maximum heating load by types of silkworm rearing houses were determined. The results obtained from the study were as follows : 1. The average heating load coefficients of NS, OS and CC type simple silkworm rearing houses were $24.1KJ/m^2-hr-^{\circ}C$, $19.8KJ/m^2-hr-^{\circ}C$, and $10.8KJ/m^2-hr-^{\circ}C$, respectively. 2. The change of heating load coefficient by progress of silkworm rearing day after reared into simple silkworm rearing house could be expressed as Fig. 4. 3. Heating degree-hour for adult silkworm rearing in Suweon district was calculated as $951.6^{\circ}C-hr$ for spring season and $610.5^{\circ}C-hr$ for autumn season. 4. Yearly heating requirement of the NS type was estimated twice more than that of the CC type. Thus, some kinds of reinforced thermal adiabatic facilities is desirable for NS type. 5. The time for maximum heating load was turned out at the 4th instar during the spring season and after the mounting during the autumn season. 6. This study was performed in Suweon district. However, the estimated and analyzed data could be adapted to the major silkworm rearing district if their meteorology data were adjusted.

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Design and Fabrication of a Surge Impedance Meter (서지임피던스 측정기의 설계 및 제작)

  • Kil, Gyung-Suk;Rhyu, Keel-Soo;Kim, Il-Kwon;Moon, Byung-Doo;Kim, Hwang-Kuk;Park, Chan-Yong
    • Journal of the Korean Society for Railway
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    • v.10 no.6
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    • pp.645-649
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    • 2007
  • Ground systems flow fault currents into the ground, and suppress Ground Potential Rise (GPR) by the current. In this paper, we designed and fabricated a surge impedance meter to analyze the ground impedance in wide frequency ranges. The meter consists of a surge generator, a high speed sample/hold (S/H) circuit and an associated electronics. The surge generator produces surge voltage up to 5kV in ranges of $50\sim500ns$. Field tests were carried out to evaluate the surge impedance meter at a driven-rod ground system. The results showed that surge impedance of ground systems should be measured by various fast surge waveforms, since the impedance increases as the rise time of applied voltage increases.

The rs196952262 Polymorphism of the AGPAT5 Gene is Associated with Meat Quality in Berkshire Pigs

  • Park, Woo Bum;An, Sang Mi;Yu, Go Eun;Kwon, Seulgi;Hwang, Jung Hye;Park, Da Hye;Kang, Deok Gyeong;Kim, Tae Wan;Park, Hwa Chun;Ha, Jeongim;Kim, Chul Wook
    • Food Science of Animal Resources
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    • v.37 no.6
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    • pp.926-930
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    • 2017
  • High-quality meat is of great economic importance to the pig industry. The 1-acylglycerol-3-phosphate-O-acyltransferase 5 (AGPAT5) enzyme converts lysophosphatidic acid to phosphatidic acid in the mitochondrial membrane. In this study, we found that the porcine AGPAT5 gene was highly expressed in muscle tissue, influencing meat characteristics, and we also identified a non-synonymous single-nucleotide polymorphism (nsSNP) (rs196952262, c.673 A>G) in the gene, associated with a change of isoleucine 225 to valine. The presence of this nsSNP was significantly associated with meat color (lightness), lower cooking loss, and lower carcass temperatures 1, 4, and 12 h after slaughter (items T1, T4, and T12 on the recognized quality scale, respectively), and tended to increase backfat thickness and the water-holding capacity. These results suggest that nsSNP (c.673A>G) of the AGPAT5 gene is a potential genetic marker of high meat quality in pigs.

Association between a non-synonymous single nucleotide polymorphism in the Complement component 9 (C9) gene and meat-quality traits in Berkshire pigs (Complement component 9 (C9) 유전자의 단일염기다형성과 버크셔 돼지 육질 형질과의 연관성 분석)

  • Ha, Jeongim;Hwang, Jung Hye;Yu, Go Eun;Park, Da Hye;Kang, Deok Gyeong;Kim, Tae Wan;Park, Hwa Chun;An, Sang Mi;Kim, Chul Wook
    • Korean Journal of Food Science and Technology
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    • v.50 no.5
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    • pp.480-485
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    • 2018
  • In this study, to identify single nucleotide polymorphisms (SNPs) associated with meat quality in Berkshire pigs, we performed RNA sequencing. A non-synonymous SNP (nsSNP) in the Complement component 9 (C9) gene was identified, and the association between meat quality traits and the C9 genotype was analyzed. The nsSNP in the C9 gene was located at c.942 G>T. In the dominant model, significant associations were observed between the SNP and meat quality traits such as CIE L, collagen content, moisture level, and $pH_{24h}$, whereas in the co-dominant model, significant associations were observed between the SNP and CIE L, collagen content, and protein content. In the recessive model, a significant association between the C9 genotype and the collagen content was observed. In addition, we identified the significant relationship between the C9 genotype and meat quality according to sex. These results indicate that the C9 SNP can be used as a genetic marker for improving pork quality.

Self-starting vector phase conjugate laser oscillator in inverted Nd:YAG

  • Kim, D.H.;Udaiyan, D.;Green, R.P.M.;Crofts, G.J.;Damzen, M.J.
    • Journal of the Optical Society of Korea
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    • v.1 no.1
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    • pp.41-43
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    • 1997
  • We report the operation of a self-adaptive vector phase conjugate laser (VPCL) oscillator which compensates intracavity polarization distortion and wavefront aberration simultaneously. The VPCL in Nd:YAG gain media produce an output with energy of 125mJ in a 20ns single-longitudinal-mode pulse at 10Hz, which is unaffected by intracavity polarization distortion.

Simultaneous Dual-Enzyme Immunoassays in a Solid Phase

  • 백세환;박순재
    • Bulletin of the Korean Chemical Society
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    • v.18 no.1
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    • pp.44-50
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    • 1997
  • A method of dual-signal generation from two different enzymes was developed and utilized to simultaneously perform dual immunoassays in a single microwell. Two enzymes selected as tracers were horseradish peroxidase (HRP) and β-galactosidase (GAL). 3, 3', 5, 5'-Tetramethylbenzidine (TMB) and chlorophenolred-β-galactopyranoside (CPRG) as chromogenic substrates for the respective enzyme were used. Although the two enzymes showed their maximum activities at distinct pH conditions (pH 5.1 for HRP and 7.5 for GAL), the enzyme reactions were able to be concurrently carried out at pH 5.75 in a dual-substrate solution without signal loss. This performance was achieved by increasing TMB concentration two-fold, introducing potassium salt as activator of GAL reaction, and extending total reaction time 50%. The signal generation method was then used for dual-enzyme immunoassays to detect antibodies with co-immobilized Hepatitis C virus antigens (core and NS5) and a Hepatitis B virus antigen (PreS(2)) in a microwell. Dose-response curves of the assays revealed cooperativity between different antigen-antibody complex formation, which suggested that dual immunoassays can only be used for qualitative screening tests unless the antigens immobilized were spatially separated.

Fabrication and Test of Hundred ns, Hundred kV Pulse Power Supply (수백ns, 수백 kV 펄스전원공급장치 제작 및 시험)

  • Park, Soung-Soo;Heo, Hoon;Kim, Sung-Chul;Kim, Sang-Hee;Kim, Sung-Hwan;Park, Young-Jung;Choi, O-Rong;Nam, Sang-Hoon;Shin, J.W.;So, J.H.;Jang, W.
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.1364_1366
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    • 2009
  • 고출력 펄스 전원으로 PFN Marx 펄스 전원공급장치를 설계 및 제작하였다. PFN Marx는 커패시터와 인덕터 그리고 스파크 갭과 전원을 공급하는 전원공급장치와 전류 인덕터로 구성하여 제작을 하였다. 여기에 사용하는 스파크 갭 스위치는 개스를 채우는 방식으로 개스의 압력을 조정하여 스위치의 스위칭 전압을 조정하여 준다. PFN Marx는 커패시터와 인덕터가 직병렬로 구성이 되며 펄스폭과 PFN의 임피던스를 결정하는 중요한 요소이다. PFN marx는 33단으로 구성을 하였으며 부하를 $100{\Omega}$으로 설계 및 시뮬레이션을 통하여 제작하였다. PFN Marx 펄스전원공급장치를 약 500 kV, 수백 ns까지 시험을 하였다. 본 논문에서는 PFN Marx 펄스전원장치의 설계 제작 및 시험에 대하여 고찰하고자 한다.

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Virtual Arrival Mechanism for IEEE 802.15.4 beacon enabled networks (비콘을 사용하는 IEEE 802.15.4 네트워크를 위한 가상 도착 메커니즘)

  • Ha, Jae-Yeol;Lee, Jong-Wook;Kwon, Wook-Hyun;Kim, Jung-Joon;Kim, Yong-Ho;Shin, Young-Hee
    • Journal of The Institute of Information and Telecommunication Facilities Engineering
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    • v.4 no.2
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    • pp.45-51
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    • 2005
  • For power constrained applications, IEEE 802.15.4 networks may be operated in beacon enabled mode with inactive period. h this paper, we propose the Virtual Arrival Mechanism (VAM) to avoid the congestion at the beginning of each contention access period (CAP). Virtual Arrival Mechanism (VAM) is a kind of traffic shaping that spread the traffics congested at the beginning of CAP into the whole CAP. By using VAM, collisions and energy consumption can be reduced. Finally, we evaluate the performance enhancement of VAM using NS-2 simulator.

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