• Journal of the Korean Institute of Intelligent Systems
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• 제17권7호
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• pp.939-944
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• 2007

Edge is a significant element to represent boundary information between objects in images. There are various edge detection methods, which are based on differential operation, such as Sobel, Prewitt, Roberts, Canny, Laplacian, and etc. However the conventional methods have drawbacks as follow : (i) insensitivity to edges with gentle curve intensity, (ii) detection of double edges for edges with one pixel width. For the detection of edges, not only development of the effective operators but also that of appropriate thresholding methods are necessary. But it is very complicate problem to find an appropriate threshold. In this paper, we propose an edge detection method based on the region separateness between objects to overcome the drawbacks of the conventional methods, and a thresholding method for the proposed edge detection method. We show the effectiveness of the proposed method through experimental results obtained by applying the proposed and the conventional methods to well-known test images.

• Journal of Life Science
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• 제26권1호
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• pp.140-145
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• 2016

Genes in multicellular organisms are transcribed in development, differentiation, or tissue-specific manners. The transcription of genes is activated by enhancers, which are transcription regulatory elements located at long distances from the genes. Recent studies have reported that noncoding RNAs are transcribed from active enhancers by RNA polymerase II (RNA Pol II); these are called enhancer RNAs (eRNAs). eRNAs are transcribed bi-directionally from the enhancer core, and are capped on the 5’ end but not spliced or polyadenylated on the 3’ end. The transcription of eRNAs requires the binding of transcription activators on the enhancer and associates positively with the transcription of the target gene. The transcriptional inhibition of eRNAs or the removal of eRNA transcripts results in the transcriptional repression of the coding gene. The transcriptional procedure of eRNAs causes enhancer- specific histone modifications, such as histone H3K4me1/2. eRNA transcripts directly interact with Mediator and Rad21, a cohesin subunit, generating a chromatin loop structure between the enhancer and the promoter of the target gene. The recruitment of RNA Pol II into the promoter and its elongation through the coding region are facilitated by eRNAs. Here, we will review the features of eRNAs, and discuss the mechanism of eRNA transcription and the roles of eRNAs in the transcriptional activation of target genes.

• Journal of Korean Society of Forest Science
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• 제103권1호
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• pp.51-58
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• 2014

Genetic diversity and genetic structures were estimated in seven natural populations of Phellodendron amurense Rupr in South Korea using ISSR markers. The average of polymorphic loci per primer and the proportion of polymorphic loci per population were 4.5 and 78.8% respectively with total 27 polymorphic loci from 6 ISSR primers. The Shannon's diversity index(I) was 0.421 and the expected heterozygosity($H_e$) was 0.285, which was similar to the heterozygosity (hs =0.287) inferred by Bayesian method. In AMOVA, 7.6% of total genetic variation in the populations was resulted from the genetic difference among populations and the other 92.4% was resulted from the difference among individuals within populations. Genetic differentiation(${\theta}^{II}$) and inbreeding coefficient(f) for total population were estimated to be 0.066 and 0.479 by Bayesian method respectively. In Bayesian clustering analysis, seven populations were assigned into three groups. This result was similar to the results of genetic relationships by UPGMA and PCA. The first group included Hwachoen, Gapyeong, Bongpyeong and Yongpyeong population, and the second included two populations in Sancheong region. Muju population was discretely assigned into the third group in spite of the geographically short distance from the Sancheong region. There was no significant correlation between genetic relationship and geographic distribution among populations in Mantel's test. For conservation of the phellodendron trees, it would be effective to consider the findings resulted from this study with ecological traits and life histories of this species.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.917-922
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• 2008

A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTS1 gene was obtained. Analysis of the ADAMTS1 gene using the porcine radiation hybrid panel indicated that pig ADAMTS1 is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTS1 as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).

• Journal of Astronomy and Space Sciences
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• 제22권4호
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• pp.419-430
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• 2005

We have carried out a statistical analysis on solar wind dynamic pressure pulses during geomagnetic storms. The Dst index was used to identify 111 geomagnetic storms that occurred in the time interval from 1997 through 2001. We have selected only the events having the minimum Dst value less than -50 nT. In order to identify the pressure impact precisely, we have used the horizontal component data of the magnetic field H (northward) at low latitudes as well as the solar wind pressure data themselves. Our analysis leads to the following results: (1) The enhancement of H due to a pressure pulse tends to be proportional to the magnitude of minimum Dst value; (2) The occurrence frequency of pressure pulses also increases with storm intensity. (3) For about $30\%$ of our storms, the occurrence frequency of pressure pulses is greater than $0.4\#/hr$, implying that to. those storms the pressure pulses occur more frequently than do periodic substorms with an average substorm duration of 2.5 hrs. In order to understand the origin of these pressure pulses, we have first examined responsible storm drivers. It turns out that $65\%$ of the studied storms we driven by coronal mass ejections (CMEs) while others are associated with corotating interaction regions $(6.3\%)$ or Type II bursts $(7.2\%)$. Out of the storms that are driven by CMEs, over $70\%$ show that the main phase interval overlaps with the sheath, namely, the region between CME body and the shock, and with the leading region of a CME. This suggests that the origin of the frequent pressure pulses is often due to density fluctuations in the sheath region and the leading edge of the CME body.

• Journal of Life Science
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• 제20권5호
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• pp.683-690
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• 2010

The yeast strains of Saccharomyces diastaticus produce one of three isozymes of an extracellular glucoamylase I, II or III, a type of exo-enzyme which can hydrolyse starch to generate glucose molecules from non-reducing ends. These enzymes are encoded by the STA1, STA2 and STA3 genes. Another gene, sporulation-specific glucoamylase (SGA), also exists in the genus Saccharomyces which is very homologous to the STA genes. The SGA has been known to be produced in the cytosol during sporulation. However, we hypothesized that the SGA is capable of being secreted to the extracellular region because of about 20 hydrophobic amino acid residues at the N-terminus which can function as a signal peptide. We expressed the cloned SGA gene in S. diastaticus YIY345. In order to compare the biochemical properties of the extracellular glucoamylase and the SGA, the SGA was purified from the culture supernatant through ammonium sulfate precipitation, DEAE-Sephadex A-50, CM-Sephadex C-50 and Sephadex G-200 chromatography. The molecular weight of the intact SGA was estimated to be about 130 kDa by gel filtration chromatography with high performance liquid chromatography (HPLC) column. Sodium dedecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed it was composed of two heterogeneous subunits, 63 kDa and 68 kDa. The deglycosylation of the SGA generated a new 59 kDa band on the SDS-PAGE analysis, indicating that two subunits are glycosylated but the extent of glycosylation is different between them. The optimum pH and temperature of the SGA were 5.5 and $45^{\circ}C$, respectively, whereas those for the extracellular glucoamylase were 5.0 and $50^{\circ}C$. The SGA were more sensitive to heat and SDS than the extracellular glucoamylase.

• The Bulletin of The Korean Astronomical Society
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• 제44권1호
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• pp.42.1-42.1
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• 2019

We observed 80 dense cores ($N(H_2)$ > $10^{22}cm^{-2}$) in the Orion molecular cloud complex which contains the Orion A (39 cores), B (26 cores), and ${\lambda}$ Orionis (15 cores) clouds. We investigate the behavior of the different molecular tracers and look for chemical variations of cores in the three clouds in order to systematically investigate the effects of stellar feedback. The most commonly detected molecular lines (with the detection rates higher than 50%) are $N_2H^+$, $HCO^+$, $H^{13}CO^+$, $C_2H$, HCN, and $H_2CO$. The detection rates of dense gas tracers, $N_2H^+$, $HCO^+$, $H^{13}CO^+$, and $C_2H$ show the lowest values in the ${\lambda}$ Orionis cloud. We find differences in the D/H ratio of $H_2CO$ and the $N_2H^+/HCO^+$ abundance ratios among the three clouds. Eight starless cores in the Orion A and B clouds exhibit high deuterium fractionations, larger than 0.10, while in the ${\lambda}$ Orionis cloud, no cores reveal the high ratio. These chemical properties could support that cores in the ${\lambda}$ Orionis cloud are affected by the photo-dissociation and external heating from the nearby H II region. An unexpected trend was found in the $[N_2H^+]/[HCO^+]$ ratio with a higher median value in the ${\lambda}$ Orionis cloud than in the Orion A/B clouds than; typically, the $[N_2H^+]/[HCO^+]$ ratio is lower in higher temperatures and lower column densities. This could be explained by a longer timescale in the prestellar stage in the ${\lambda}$ Orionis cloud, resulting in more abundant nitrogen-bearing molecules. In addition to these chemical differences, the kinematical difference was also found among the three clouds; the blue excess, which is an infall signature found in optically thick line profiles, is 0 in the ${\lambda}$ Orionis cloud while it is 0.11 and 0.16 in the Orion A and B clouds, respectively. This result could be another evidence of the negative feedback of active current star formation to the next generation of star formation.

• Journal of Plant Biotechnology
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• 제2권1호
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• pp.43-49
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• 2000

An efficient and reliable Agrobacterium transformation procedure based on TDZ (thidiazuron)-induced organogenesis was established and applied to six Brazilian eggp1ant varieties. Optimum transgenic plants recovery was achieved upon the study of the following parameters affecting transformation efficiency, using F-100 variety as a model: i) explant source; ii) pre-culture period; iii) physical state of the pre-culture medium and iv) coculture conditions. The highest frequency of kanamycin-resistant calli derived from leaf explants (5%) was obtained without a pre-culture period and co-cultivation for 24 h in liquid medium followed by five days on solid RM (regeneration medium). For cotyledon explants, best results were achieved upon a pre-culture of 24 h in liquid RM and a co-cultivation period of 24 h in liquid RM followed by three days in solid RM, resulting in a transformation Sequency of 22.7%. Kanamycin-resistant organogenic calli were also obtained from cultivars Emb, Preta Comprida, Round nose Shaded, Campineira and Florida Market. The expression pattern of an epidermis-specific promoter was studied using transformants expressing a chimaeric construct comprised by the promoter Atgrp-5 transcriptionally fused to the coding region of the gus gene. The expression pattern was similar to that previously observed in tobacco and Arabidopsis thaliana, with preferential expression at the epidermis and the stem phloem. These results support the idea that the Atgrp-5 promoter can be used to drive defense genes in these tissues, which are sites of pathogen interaction and spread, in programs for the genetic improvement of eggplant.

• Publications of The Korean Astronomical Society
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• 제27권4호
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• pp.335-338
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• 2012

We present the $18{\mu}m$ luminosity function (LF) of galaxies at 0.006 < z < 0.8 (the average redshift is ~ 0.04) using the AKARI mid-infrared All-Sky Survey catalogue. We have selected 243 galaxies at $18{\mu}m$ from the Sloan Digital Sky Survey (SDSS) spectroscopic region. These galaxies then have been classified into five types; Seyfert 1 galaxies (Sy1, including quasars), Seyfert 2 galaxies (Sy2), low ionization narrow emission line galaxies (LINER), galaxies that are likely to contain both star formation and Active Galactic Nuclei (AGN) activities (composites), and star forming galaxies (SF) using optical emission lines such as the line width of $H{\alpha}$ or the emission line ratios of [OIII]/$H{\beta}$ and [NII]/$H{\alpha}$. As a result of constructing the LF of Sy1 and Sy2, we found the following results; (i) the number density ratio of Sy2 to Sy1 is $1.64{\pm}0.37$, larger than the results obtained from optical LF and (ii) the fraction of Sy2 in the entire AGN population may decrease with $18{\mu}m$ luminosity. These results suggest that most of the AGNs in the local universe are obscured by dust and the torus structure probably depends on the mid-infrared luminosity.

• Transactions of the Korean Society of Mechanical Engineers B
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• 제21권9호
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• pp.1115-1125
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• 1997

Experiments have been conducted to obtain local and average heat transfer coefficients associated with impingement of a row of circular, free surface-water jets on a constant heat flux surface. Nozzle arrays are a row of 3 jets (nozzle dia.=4.6 mm) and a row of 5 jets (nozzle dia.=3.6 mm), and the nozzle configuration is Reverse cone type revealed good performance in heat transfer. Nozzle-to-plate spacings ranging from 16 mm to 80 mm were investigated for two jet center to center spacings 25 mm and 37.5 mm in the jet velocity of 3 m/s (R $e_{D}$=27000) to 8 m/s (R $e_{D}$=70000). For a row of 3 jets and a row of 5 jets, the stagnation heat transfer of the central jet is lower than that of adjacent jets. In the wall jet region between jets, for small nozzle-to-plate spacing and large jet velocity, the local maximum in the Nusselt number was observed, however, for small jet velocity or large nozzle-to-plate spacing, the local maximum was not observed. Except for the condition of $V_{O}$=8 m/s and H/D=10, the average Nusselt number reveals the following ranking: a row of 5 jets, a row of 3 jets, single jet. For a row of 3 jet, the maximum average Nusselt number occurs at H/D=8 ~ 10, and for a row of 5 jets, it occurs at H/D=2 ~ 4. Compared with the single jet, enhancement of average heat transfer for a row of 3 jets is approximately 1.52 ~ 2.28 times, and 1.69 ~ 3.75 times for a row of 5 jets.ets.s.