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http://dx.doi.org/10.14578/jkfs.2014.103.1.51

Genetic Diversity and Genetic Structure of Phellodendron amurense Populations in South Korea  

Lee, Jei-Wan (Division of Forest Genetic Resources, Korea Forest Research Institute)
Hong, Kyung-Nak (Division of Forest Genetic Resources, Korea Forest Research Institute)
Kang, Jin-Taek (Center for Forest & Climate Change, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.103, no.1, 2014 , pp. 51-58 More about this Journal
Abstract
Genetic diversity and genetic structures were estimated in seven natural populations of Phellodendron amurense Rupr in South Korea using ISSR markers. The average of polymorphic loci per primer and the proportion of polymorphic loci per population were 4.5 and 78.8% respectively with total 27 polymorphic loci from 6 ISSR primers. The Shannon's diversity index(I) was 0.421 and the expected heterozygosity($H_e$) was 0.285, which was similar to the heterozygosity (hs =0.287) inferred by Bayesian method. In AMOVA, 7.6% of total genetic variation in the populations was resulted from the genetic difference among populations and the other 92.4% was resulted from the difference among individuals within populations. Genetic differentiation(${\theta}^{II}$) and inbreeding coefficient(f) for total population were estimated to be 0.066 and 0.479 by Bayesian method respectively. In Bayesian clustering analysis, seven populations were assigned into three groups. This result was similar to the results of genetic relationships by UPGMA and PCA. The first group included Hwachoen, Gapyeong, Bongpyeong and Yongpyeong population, and the second included two populations in Sancheong region. Muju population was discretely assigned into the third group in spite of the geographically short distance from the Sancheong region. There was no significant correlation between genetic relationship and geographic distribution among populations in Mantel's test. For conservation of the phellodendron trees, it would be effective to consider the findings resulted from this study with ecological traits and life histories of this species.
Keywords
Phellodendron amurense; genetic diversity; genetic structure; genetic resources; conservation; ISSR; Bayesian inference; AMOVA;
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