• Journal of Animal Environmental Science
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• v.15 no.3
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• pp.231-240
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• 2009

In this research pig house facilities what are 3,029 houses hold over 1000 heads were surveyed by scale and province. Full-time farms hold over 1000 heads breed total 7,229,892 heads. And farms breed 2,386.9 heads averagely. Pig houses were constructed august 1995 averagely. Each of houses have $3,017.2\;m^2$ scale. The construction type of pig house was winch-curtain type 77.2% which was most popular, confined type 51.3%, litter type 7.4% and loft type 4.6%. The winch-curtain type was popular than windowless type in pig farms which have 1,000-1,999 heads. But pig house construction type which have more than over 10,000 heads was windowless type more than winch-curtain type. Manure removing type was slurry 72.3% and scraper 38.5% in farms which have 1,000-1,999 heads. Manure removing type was slurry 83.3% in farms which have over 10,000 heads. Proportion of roof type of pig house was slate 51.2%, panel 46.1%. But in middle or small farms, slate type was only 25.0%. Proportion of wall type of pig house was 41.9%, block 21.9%, concrete 7.6%, winch-curtain 6.3%, and bnck 5.9%. Ventilation type of pig house was natural winch 46.1%, mechanical windowless 69.8% and mixed type 53.1%. So, mechanical windowless type was popular than natural winch type. Especially the farm scale is bigger the mechanical widowless type was more. Utilization period of pig house was 8.1 years about automatic feeder, 8.3 years about waterer, 8.2 years about electric facilities and 9.0 years about floor material. Thus, almost of facilities were used at least 8 years.

• The Journal of Internal Korean Medicine
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• v.34 no.2
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• pp.113-121
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• 2013

Objectives : This study aimed to find out whether acupuncture at various acupoints shows any effects on appetite by regulating neurotransmitters in the hypothalamus through the expression of NPY and LR in the PVN via immunohistochemistry. Methods : Male Sprague-Dawley rats were divided into eight groups of five mice each. The rats in the acupuncture groups were treated with acupuncture at respective acupoints, twice per a day for 3 days. The animals were sacrificed 72 h after commencement of the experiment, the brains being dissected into serial coronal sections. Expressions of neuropeptide Y and leptin receptor in the hypothalamus were assessed by immunohistochemistry. Results : NPY expression in the PVN was enhanced and LR expression in the PVN was decreased by food deprivation. NPY immunoreactivity in the PVN of the food-derived rats was decreased by acupuncture at the auricular acupoint, Zusanli-acupoint, a and non-acupoint. However, acupuncture at the auricular acupoint showed most potent suppressing effect on NPY expression in the PVN of food-deprived rats. LR expression in the PVN decreased following food deprivation, and auricular acupuncture increased LR expression in the PVN of food-deprived rats. In normal conditions (fed state), LR expression in the PVN was not changed by acupuncture treatment at several sites. Conclusions : From this study, we have shown that acupuncture at the auricular acupoint exerts the most potent appetite suppressing effect on the food restriction state.

• Journal of Food Hygiene and Safety
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• v.31 no.3
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• pp.186-193
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• 2016

An analytical method was developed for the determination of oxathiapiprolin in agricultural commodities. Oxathiapiprolin is a new oomycide (fungicide of piperidinyl thiazole isoxazoline class) which controls downy mildew in cucurbits caused by Pseudoperonospora cubensis (oomycete plant pathogen). Agricultural commodities were extracted with acetonitrile and partitioned with dichloromethane to remove the interference, adjusting pH between 9 and 10 by 1 N sodium hydroxide. After purification by silica SPE cartridge to clean up the interference of organic compounds, they were finally quantified by HPLC-UVD (high performance liquid chromatograph ultraviolet detector) using a wavelength at 260 nm and confirmed by LC-MS (liquid chromatograph mass spectrometer) in electro-spray ionization positive ion mode. The standard calibration curve was linear with coefficients of determination ($r^2$) 1.00 over the calibration ranges (0.025-2.5 mg/L). Recoveries were ranged between 86.7 to 112.7%, with relative standard deviations less than 10% at three concentration levels (LOQ, 10LOQ, and 50LOQ) performing five replicates. The overall results were determined and estimated according to the CODEX guidelines (CAC/GL40). The proposed method for determination of oxathiapiprolin residues in agricultural commodities can be used as an official method.

• Korean Journal of Ichthyology
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• v.13 no.1
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• pp.40-49
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• 2001

The ichthyofauna and fish community of the Dongjin River system were surveyed at 21 sites from October 1998 to September 1999. We confirmed the presence of 53 species in 14 families belonging to seven orders. Thirty-two species of cyprinid fishes accounted for 60.4% of the total, four species of cobitid and gobiid fishes 7.5% of the total, and two species of bagrid and centrarchid fishes for 3.8% of the total. The dominant species in the Dongjin River system was Zacco platypus (relative abundant 26.0%), the subdominant species was Carassius auratus (14.7%). The dominant and subdominant species in each stream of the Dongjin River system were determined. In the Dongjin River, the dominant species was Z. platypus (40.6%), the subdominant Acheilognathus lanceolatus (20.5%); in Wonpyeong stream, Pseudorasbora parva (22.6%) and Hypomesus olidus (13.2%); in Gobu stream C. auratus (67.5%) and Cyprinnus carpio (15.4%). Fourteen of the 53 species from the the Dongjin River system comprised 28.6% of the total Korean endemic species; seven rare species accounted for less than 0.1% of the total. Four exotic fish species were found: Cyprinus carpio (Islaeli carp), Carassius cuvieri, Micropterus salmoides and Lepomis macrochirus. The species dominance, diversity, and evenness index of the fish community from the Wonpyeong stream were 0.12, 1.08, and 0.91, respectively, indicating better conditions than those of the Dongjin River and the Gobu stream. The fish community of the Wonpyeong stream showed closer relationship to that of the Dongjin River than to that of the Gobu stream.

• The Korean Journal of Pharmacology
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• v.32 no.1
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• pp.13-21
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• 1996

Intracellular calcium concentration ($[Ca^{2+}]_i$) may play a crucial role in a variety of neuronal functions. Here we report that in primary culture of mouse cerebellar granule cells nicotinic acetylcholine receptors (nAChRs) are expressed in a specific developmental stage and involved in the regulation of intracellular calcium homeostasis. Nicotine-mediated calcium responses were measured using $^{45}Ca^{2+}$ or fluorometrically using the calcium-sensitive fluorescent dye fura-2. Maximal uptake of $^{45}Ca^{2+}$ evoked by nicotine in mouse cerebellar granule cells were revealed $8{\sim}12$ days in culture. In contrast, nicotine did not alter the basal $^{45}Ca^{2+}$ uptake in cultured glial cells. In cerebellar granule cells nicotine-evoked $^{45}Ca^{2+}$ uptake was largely blocked by the NMDA receptor antagonists. Glutamate pyruvate transaminase (GPT). which removes endogenous glutamate, also prevented nicotine effects, implying the indirect involvement of glutamate in nicotine-mediated calcium responses. Fluorometric studies using fura-2 showed two phases of nicotine-evoked $[Ca^{2+}]_i$ rises: the initial rising phase and the later plateau phase. Interestingly, the NMDA receptor antagonists and GPT appeared to inhibit only the later plateau phase of nicotine-evoked $[Ca^{2+}]_i$ rises. The present results imply that nicotine mediated $^{45}Ca^{2+}$ uptake and $[Ca^{2+}]_i$ rises are attributed to the calcium fluxes through both nAchRs and NMDA receptors in a time-dependent manner. Consequently, nAChRs may play an important role in neuronal development by being expressed in a specific developmental stage and regulating the intracellular calcium homeostasis.

• Korean Journal of Environmental Biology
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• v.25 no.4
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• pp.303-312
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• 2007

In order to investigate the dynamics of phytoplankton standing crops affecting by environmental factors, biological and environmental factors, this study was examined in the marine ranching ground of Tongyeong coastal waters from 2000 to 2007. During the study, mean water temperature and salinity were 16.7$^{\circ}C$ and 32.9 psu, respectively. pH, DO and SS varied from 7.81$\sim$8.09, 3.02$\sim$8.97 mg $L^{-1}$ and 2.7$\sim$32.2 mg $L^{-1}$, respectively. Mean concentrations of dissolved inorganic nitrogen, phosphate and silicate were 21.75 ${\mu}M$, 0.90 ${\mu}M$ and 14.38 ${\mu}M$, respectively. Chlorophyll a concentrations varied from 0.02 ${\mu}g$ $L^{-1}$ to 25.29 ${\mu}g$ $L^{-1}$ with mean a value of 2.0 ${\mu}g$ $L^{-1}$. These factors did show significant differences on each layer and season, while did not show on the sampling stations. Phytoplankton standing crops varied from $4.21\times10^3$ cells $L^{-1}$ to $1.44\times10^6$ cells $L^{-1}$ with a mean value of $1.92\times10^5$ cells $L^{-1}$. Especially, variations of phytoplankton standing crops had an unimodal pattern as only bloomed in autumn rather than a bimodal pattern as generally bloomed in spring and autumn. In results of stepwise multiple regression analysis, the coefficient of determination $(R^2)$ for total standing crops was 0.35 and the standing crops were affected by water temperature, salinity, phosphate and silicate. The factors affected were different seasonally; water temperature in spring, salinity in summer, water temperature, salinity and silicate in autumn and water temperature, salinity and suspended solids in winter. Therefore, the results from the statistical analysis showed that the environmental factors influencing on the variations of the phytoplankton standing crops were predominantly water temperature and salinity.

• Korean Journal of Organic Agriculture
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• v.18 no.4
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• pp.527-539
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• 2010

This study was conducted to determine the effect of probiotics as an alternative for antibiotics on growth performance, nutrient digestibility, noxious gas emission and fecal microbial population in growing piglets. A total of 96 piglets ($22.5{\pm}1.3$kg average body weight) were allotted to 3 different treatment groups and replicated 4 times with 8 piglets per replicate in randomized complete block design. Treatments were T1) (Control, basal diet+0.2% antibiotics), T2) 0.2% probiotics complex and T3) 0.3% Bacillus probiotics. During the whole experiment period, there were no differences (p>0.05) in average daily gain (ADG), average daily feed intake (ADFI) and feed efficiency. However, digestibility of dry matter, crude protein, ether extract, nitrogen free extract and crude ash were showed higher in probiotics groups (T2 and T3) than those of control. In noxious gas emission, ammonia, amine, hydrogen sulfide and mercaptan were significantly (p<0.05) reduced in T2 and T3 treatments compared to those in control. Moisture content of feces was not significantly different among treatments. The colony forming units (CFU) of total bacteria, E. coli and thermoduric bacteria in feces were significantly different among treatments. The CFU of total bacteria, E. coli and thermoduric bacteria in T3 treatment were reduced by feeding probiotics B. From this study, we suggest that probiotics A and B are likely able to improve the growth performance and nutrients digestibility, reduce noxious gas emission and change the fecal microbial composition in growing piglets.

• Journal of Chest Surgery
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• v.30 no.4
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• pp.363-372
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• 1997

One hundred forty-four patients underwent operation for coarctation of the aorta at Seoul National University Children's Hospital between June 1986 and Decembsr 1995. Age ranged 0.1 to 191 months. Of these 78.5%(113) were infants. We classified the patients in terms of the anatomic location of coarctatiln and the associatCd anomalies(I[401= primary coarctation, 11(741=isthmic hypoplasia, lIIf30)=tubular hypoplasia involving transverse arch, Ar63 =with ventricular septal defect, B(28)=with other major cardiac defects). Subcalvian flap coarctoplasty(60), resection & anastomosis(44), extended aortoplasty(26), and onlay patch(14) were used as surgical methods. Overall operative mortality was 16.0(23/144)%. The hospital mortality was signific'antly higher in patheints with type 111, subtype B, younger age(under 3 months), extended aortoplasty(p(0.01). However, one-stage total repair in patients with subtype A or B were not found to be a predictor of hospital death. Restenosis had occured in 18 patients among 121 survivals(14. 9%). The mean follow-up period was 29.1 $\pm$28.8(0~129.2) months. Preoperative, immediate postoperative(within 3 months after operation) and postoperative(later than 6 months after operation) echocardiographic data on the dimensions of ascending aorta(AA), transverse arch(TA), an4 aortic isthmus(Al) were available in 77 patients(I=20, ll=42, 111= 15). Preoperative and postoperative aortic isthmus(All) and tra sverse arch indices(TAI), defined as TAIAA & AIIAA respectively, were compared. Immediate postoperative All in type 1, II and TAI in type 111 were significantly smaller in stenotic than non-stenotic group suggesting incomplete relieves of stenotic segment Younger age, subclavian coarctoplasty in patient under 3 months of age were round to be the risk factors for restenosis in this series. In conclusion, We found that aortic arch index and transverse arch index can be a useful tool to figure out the anatomic and clinical characteristics of the patients with aortic coarctation, and that anatomy, associated anomalies, age, and surgical methods may influence the surgical outcome of the coarctation repair.

• Korean Journal of Weed Science
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• v.10 no.4
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• pp.269-276
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• 1990

Weed species belonging to the broadleaf family prevailed in the experimental sites followed by Cyperucea and grass family. In terms of life cycle, the dominant weed species were the perennial weeds rather than annual weeds. Most of weeds were emerged between 30-60days after transplanting in Chuncheon and between 40-60 days after transplanting in Hongcheon and Hoengseong areas. Among agronomic characteristics of rice affected by weed emergence were decrease of plant height by 2-4㎝, panicle number by 1.3-2.9, spkelet by 3. 7-7.5, ripening rates by 3.3-6.5%, and milled yield by 12-17%.

• Korean Journal of Weed Science
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• v.10 no.2
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• pp.83-92
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• 1990

Experiment was conducted to establish the weed control system on the paddy fields by investigating species, period and amount of weeds in Gangwon province. Four locations were selected for these experiments : West plain area, Chunchon(74m above the sea level), mid-mountainous area, Hongchon(300 m), high alpine area, Hoengsong(450m), and east sea area, Myungju(14m). Weed emergence was investigated 6 times from 10 days after transplanting to 60 days with 10 days interval. From the experiments stated above, the results were summarized as follows ; The identity of weed species was small in grass family, but broad-leaf family was numerous. Also the dominant weed species was generally the perenial weeds. The weed emergence was initiated from 10 days after translanting. The amounts of weed counted the most in Chunchon. A large number of weeds were emerged between 30-60 days after transplanting in Chunchon and between 40-60 days after transplanting in Hongchon and Hoengsong. Among agronomic characteristics affected by weed emergence during rice development, plant height was decreased by 2~4cm, panicle number by 1.3~2.9, spikelet by 3.7~7.5, ripening rates 3.3~6.5%, and milled yield by 12~17%, respectively.