• 생명과학회지
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• 제14권4호
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• pp.593-607
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• 2004

유해성 적조생물 Cochlodinium polykrikoides/Gyrodinium aurelum을 포함한 43 종류의 와편조류를 대상으로 SSU 부위 유전자를 분석했다. 유전자 염기서열에 의거한 상호 계통수는 parisomny, distance, maxium 방법으로 실행했다. Noctilura scintillans, Gonyaulax spinifera와 Crythecodinium cohnii 종들은 와편모조류 중 가장 유전적으로 먼 것으로 보였다. Alexandrium과 Symbiodinium 종간의 bootstrap는 70% 이상의 상호 단일 계통도를 보인 반면에, Gymnodinium과 Gyrodinium은 근립절약계수와 최대 유사도 방법에서 다형 계통도를 나타내었다. Gyroddinium aurelum과 G. dorsum/G. galathenum의 유전적 분화율은 7.4% (45 bp) 였고, G. aurelum과 G. mikimotoi 상호간에는 0.9% (5bp) 밖에 나타나지 않았다. 또한 C. polykrikoides와 G. aurelum도 5.2% (31bp)로 낮은 유전적 분화율을 보였다. 계통도를 분석한 결과 G. aureolum과 C. polykrikoides는 Gyrodinium 보다 Gymnodinim 속에 훨씬 더 근접하게 나타났다.

• Journal of Microbiology
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• 제42권4호
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• pp.305-314
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• 2004

An exopolysaccharide-producing microalgal dinoflagellate was isolated from a red-tide bloom and des­ignated strain KG03. A bacteria-free culture of strain KG03 was achieved using a modified wash with phototaxis and antibiotic treatment. Combined treatment with neomycin and cephalosporin was the most effective for eliminating the bacteria associated with the microalgae. Strain KG03 was identified as Gyrodinium impudicum by analyzing the ITS regions of the 5.8S rDNA, 18S rDNA, morphological phenotype and fatty acid composition. The exopolysaccharide production and cell growth in a 300-ml photobioreactor were increased 2.7- and 2.4-fold, respectively, compared with that in a flask culture at the first isolation step.

• Fisheries and Aquatic Sciences
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• 제2권1호
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• pp.66-77
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• 1999

The sequences coding for the 5.8S rDNA and the internal transcribed spacers (ITS1 and ITS 2) from the isolates of nine isolates of Gyrodinium impudicum and two isolates of Gymnodinium sanguineum species were amplified, sequenced and compared with the previously known Alexandrium species and Gymnodinium catenatum. The genetic distance analyses based on the sequence alignment indicated that Gymnodinium catenatum and Gyrodinium impudicum species were some related, Alexandrium species was distant. G. catenatum and G. sanguineum were quite separate, but these two species belonged to the same genus. G. impudicum and G. catenatum forming the closet cluster showed some variation in the alignment of ITS regions. The length of ITS1 varied more than that of ITS2 and the length of ITS1 and ITS2 was different for each G. impudicum, Gymnodinium and Alexandrium species. Also, the length of ITS1 was shorter than that of ITS2. However, on the sequences of G. sanguineum, the length of ITS1 was longer about 23 nucleotides than that of ITS2. The phylogenetic analysis and rDNA similarity of G. impudicum and G. catenatum $(59\%)$ is higher than the that of G. catenatum and G. sanguineum $(55\%)$. It was thought that the phylogenetic analysis and the genetic distance revealed that G. impudicum and G. catenatum were clearly different species and G. impudicum may belong to the genus of Gymnodinium.

• 한국해양학회지
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• 제27권4호
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• pp.268-276
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• 1992

해파리류의 동물플랑크톤에 대한 포식 파급효과가 식물플랑크톤의 대발생에 어떠 한 영향을 미치는 가를 연구하기 위해, 해파리류, 동물플랑크톤, 식물플랑크톤으로 이 어지는 먹이연쇄내에서 의 상호작용을 미국 뉴욕주에 있는 Reeves만에서 쌍편모 조류 인 Gyrodinium aureolum의 대발생이 있었던 기간을 전후한 1989년 3월 중순에서 7월 초순사이에 조사하였다. 동물플랑크톤의 최대 개체수는 해파리류의 개체수가 감소하기 시작한 4월 중순에서 5월 초사이에 관찰되었으며, 이때 식물플랑크톤의 생물량은 낮게 나타났다. 한편 해파리류의 개체수가 다시 증가하였던 5월 중순부터 동물플랑크톤은 개체수의 감소를 보인 보면, 식물플랑크톤은 대발생을 시작하였다. 이와 같은 결과는 2차 소비자인 해파리류의 포식이 1차 소비자인 돈물플랑크톤의 식물플랑크톤에 미치는 섭식압력의 감소 효과에서 비롯된 것으로 나타났다. 한편, 탄소동위원소를 사용하여 측정한 동물플랑크톤의 섭식률은 대발생 전후에 비해 대발생 중에 현저하게 낮아, 동 물플랑크톤들이 대발생을 일으킨 G. aureolum을 먹이로서 선호하지 않는 것으로 밝혀 졌다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.615-620
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• 2003

Gyrodinium impudicum strain KG03의 세포외 다당류인 p-KG03은 황함유 다당류로 항바이러스 검색 결과, 조사대상 바이러스 중 특히 뇌심근 경색바이러스 (encephalomyocarditis virus; EMCV)에 항바이러스 활성을 가지는 것으로 조사되었다.

• ALGAE
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• 제22권4호
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• pp.273-286
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• 2007

Records of selected gymnodinioid dinoflagellates from the open waters in the vicinity of the Kuroshio and Oyashio Currents, the Philippine, Celebes, Sulu and South China Seas, western and central equatorial and southeast Pacific Ocean are described and illustrated. The species Gymnodinium fusus Schütt, Gyrodinium falcatum Kofoid et Swezy, G. caudatum Kofoid et Swezy, G. sugashimanii J. Cachon et al. and Pseliodinium vaubanii Sournia are considered to be morphotypes of a single species, that until further studies can establish the correct genus, are named G. falcatum. This study is the first to record individuals of G. falcatum with very long curly extensions. Other gymnodinioid dinoflagellates that showed bifurcated hyposomes may be related to Gyrodinium bifurcatum Kofoid et Swezy or cells of thecate dinoflagellates exuviated from their thecae. Some specimens showed a rigid cover, although no discernible thecal plates. In this group, the most common species was Ptychodiscus noctiluca Stein and, for the first time, a micrograph of a tentative specimen of the genus Berghiella Kofoid et Michener is reported. The validity of the genera Berghiella and Balechina Loeblich Jr. et Loeblich III with thick cell covers is discussed. Several species with apical extensions, other unknown taxa with distinctive shapes, and colonial forms are illustrated. The diversity of gymnodinioid dinoflagellates is underinvestigated in the open ocean.

• Journal of the korean society of oceanography
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• 제34권4호
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• pp.214-219
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• 1999

Cells of the dinoflagellate Gyrodinium impudicum form characteristic chains, which are associated with sugar accumulated on the cell surface. To resolve the relationship between chain-formation and cell surface sugar accumulation, confocal microscopy was used to observe sugar accumulation points in the vegetative cells and long chain-forming cells of G. impudicum cells treated with fluorescent-tagged ConA. In the stationary and exponential phases, both vegetative cells and chain-forming cells were similar to each other in fluorescent intensity. There was no evidence that chain-forming cells had a specific location for sugar accumulation on the cell surface. Most of the cells formed 2-cell chains one day after inoculation, but longer chains consisting of 4-8 cells increased markedly in 4day and 8 day cultures. Gyrodinium impudicum chains usually consist of more than four cells, and chains of 8 or even 16 cells can be observed in mature cultures. Temperature played an importantrole in chain-formation of the cells, threshold temperature for the development of long chain-formation was 19 $^{\circ}$C.

• ALGAE
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• 제36권1호
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• pp.37-50
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• 2021

Heterotrophic dinoflagellates Gyrodinium spp. are one of the major grazers of phytoplankton in many coastal waters. Gyrodinium dominans, G. jinhaense, and G. moestrupii have similar morphologies but different edible prey species. To explore the variations in the ecological niches of these three species, we investigated their spatial-temporal distributions in Korean waters. Because of the high similarity in morphology among these three Gyrodinium species, we used real-time polymerase chain reactions to quantify their abundance in water samples that were seasonally collected from 28 stations along the Korean Peninsula from April 2015 to October 2018. Cells of G. dominans were found at all sampling stations, G. jinhaense at 26 stations, and G. moestrupii at 22 stations, indicating that all three species were widely distributed in Korea. Furthermore, all three species displayed strong seasonal distributions. The largest numbers of the stations where G. dominans and G. jinhaense cells were present were found during the summer (26 and 23 stations, respectively), but that for G. moestrupii was found in the autumn (15 stations). The abundance of G. dominans was positively correlated with that of G. jinhaense, but not with that of G. moestrupii. The highest abundances of G. dominans (202.5 cells mL-1) and G. jinhaense (20.2 cells mL-1) were much greater than that of G. moestrupii (1.2 cells mL-1). The highest abundances of G. dominans and G. jinhaense were found in July, whereas that of G. moestrupii was found in March. The abundances of G. dominans and G. jinhaense, but not G. moestrupii, were positively correlated with water temperature. Therefore, the spatial-temporal distributions of G. dominans and G. jinhaense were closer than those of G. moestrupii and G. dominans or G. jinhaense. This differs from results based on the relative differences in ribosomal DNA sequences and the types of edible prey reported in the literature. Thus, the variations in spatial-temporal distributions and prey species of these three Gyrodinium species suggest that they may have different ecological niches in Korean coastal waters.

• 환경생물
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• 제35권3호
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• pp.273-288
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• 2017

An investigation focusing on the unrecorded and taxonomically undescribed indigenous has been done since 2006. Samples were collected from various sites in the coastal and offshore waters of Korea as well as around Jeju Island. Since 2008, 16 unrecorded species belonging to the family Gymnodiniaceae have been found. The species were as follows: Amphidinium thermaeum (2015), Cochlodinium convolutum (2015), C. strangulatum (2015), Gymnodinium abbreviatum (valid name: G. gracile), G. arenicola (2015), G. gracile (2015), G. dorsalisulcum (2015), G. microreticulatum (2014), G. micrum (2016) (valid name: Karlodinium micrum), G. pyrenoidosum (2016), G. simplex (2015), G. veneficum (2016) (valid name: Karlodinium veneficum), Gyrodinium aureum (2015), G. fusiforme (2015), G. dominans (2014), and Nusuttodinium latum (2016) (valid name: Amphidinium latum). (The numbers in parentheses refer to the year that the species was found). These species were newly recorded in Korean waters in this study.

• 해양환경안전학회:학술대회논문집
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• 해양환경안전학회 2009년도 춘계학술발표회
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• pp.155-155
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• 2009