• Journal of Life Science
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• v.18 no.4
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• pp.509-514
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• 2008

Tremendous natural product extracts were used as a herb medicine remedy or therapy for centuries. Because these extracts have various biological activities, we examined the effects of Gryllotalpa orientalis extract for anti-oxidant and anti-inflammation activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP), hydroxy radical scarvenging and cyclooxygenase-2 promoter assays. Gryllotalpa orientalis extracts were prepared from solvents such as distilled water (DW), dimethyl sulfoxide (DMSO), ethanol and methanol. The results showed that Gryllotalpa orientalis extracts have potent DPPH (methanol extract), FRAP (DW extract) and hydroxy radical scavenging (DW and methanol extracts) activity than any other extracts used. A significant inhibition of cyclooxygenase-2 (Cox-2) promoter activity was detected in the presence of DMSO extract or ethanol extract. Collectively, the present results suggested that Gryllotalpa orientalis extract could be used for anti-oxidant and/or anti-inflammation agent for human or agricultural purposes.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.1
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• pp.127-130
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• 2004

We describe here the cloning and characterization of a cDNA encoding a putative myosin light chain-2 (MLC-2) from the mole cricket, Gryllotalpa orientalis. The G. orientalis MLC-2 cDNA sequences comprised of 615 bp with 205 amino acid residues with a calculated molecular weight of approximately 23 kDa. The deduced protein sequence of G. orientalis MLC-2 cDNA showed 64% and 54% identity to Drosophila melanogaster MLC-2 and D. yakuba MLC-2, respectively. Northern blot analysis confirmed the muscle-specific expression of G. orientalis MLC-2.

• International Journal of Industrial Entomology and Biomaterials
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• v.25 no.2
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• pp.175-179
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• 2012

The present study is to develop the rearing method of Gryllotalpa orientalis. In total 429 of G. orientalis were collected from the field rearing cage ($25m^2$) in 2012. Its sex ratio was 1: 1.15(Female : Male). Survival rate of the mole crickets was 94.4~86.1% with the artificial diets formulated for the present study. Successful oviposition rate was 20, 20 and 80% for one, two and three pairs of adult crickets, respectively, from the indoor rearing. The mean number of hatchlings was $11.8{\pm}21.7$, $15.7{\pm}26.4$ and $25.8{\pm}38.8$, and the mean number of dead hatchlings 1.2, 1.7 and 1.2. The mortality of nymphs on horticultural soil and clay sand mixed with ocher was 18.3 and 10.0%, respectively. The mortality of nymphs in circular and rectangular cages was, respectively, 60 and 40%.

• Journal of Ginseng Research
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• v.13 no.1
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• pp.119-122
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• 1989

phenological study on the oviposition, emergence, and flight activity of the African mole cricket adult has been made to obtain basic information for management of pest populations in ginseng fields. The flight activity, as monitored by the blarklight trap, seemed to be initiated depending on the sunset time and lasted about 2-2.5 hours. The trap data (1984-1988) showed that the adult flight of the species occurred twice a year, from early May to late June(Spring flight) and from late August to mid October(Fall flight) during which usually more crickets were trapped than during the former period. The number of females trapped was greater than that of males regardless to the flight period, i.e., females comprised 72.2%, 83.9%, and 73.3% of the total catches in 1984, 1985, and 1986, respectively. Adults emerged from late August to mid October and laid eggs from mid May to mid July the next year, indicating that the spring and fall flights correspond to the oviposition and emergence period, respectively.

• Korean journal of applied entomology
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• v.32 no.1
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• pp.76-82
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• 1993

Phonotaxis of the African mole cricket, Gryllotalpa africana palisot de Beauvois, was investigated in 1990 and 1992 at the agronomy Experiment Station of Korea Ginseng & Tobacco Research Institute in Hwaseong-gun, Kyonggi-do, Male adults produced calling sounds (calling songs) through the openings of subsurface burrows. Intensities of the sound were 77-80 dB at 15 cm above the openings. When tape recordings of male calling songs were broadcasted outdoors at 105-110 dB by two horn speakers installed at the center of a 1.4 m diameter-funnel, flying adults were attracted for 1.5 hours from about 30 minutes after sunset. Among attracted adults, 14.3-16.9% landed in the funnel, and 65.7-74.7% landed on the ground within 2m form the sound source. Females were 66.7-74.3%, which seemed to be due to the sex ratio of the population in the field. Adults landing in the funnel and at the distance of within 2m from the center of the funnel were tend to be a little more than those attracted to a blacklight trap.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.1
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• pp.87-92
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• 2003

We describe here the cloning, expression and characterization of a cDNA encoding a putative chemosensory protein (CSP) from the mole cricket, Gryllotalpa orientalis. The G. orientalis chemosensory protein cDNA sequences comprised of 384 bp with 128 amino acid residues. The G. orientalis chemosensory protein showed 75.4% protein sequence identity to the Locusta migratoria CSP, Northern blot analysis revealed that signal was stronger in head than leg and cuticle, indicating that the head part containing antennae is a main site for G. orientalis chemosensory protein synthesis. The cDNA encoding G. orientalis chemosensory protein was expressed as approximately 12 kDa polypeptide in baculovirus-infected insect cells.

• International Journal of Industrial Entomology and Biomaterials
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• v.6 no.2
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• pp.157-162
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• 2003

The glutathione S-transferase (GSTs) are enzymes responsible for the protection of cells from chemical toxicants and oxidative stress. We describe here the cDNA sequence and mRNA expression of a putative GST from the mole cricket, Gryllotalpa orientalis. The G. orientalis GST cDNA sequences comprised of 621 bp encoding 207 amino acid residues. The multiple sequence alignment of G. orientalis GST gene with other known insect GSTs showed several conserved residues that may be essential for the enzymatic activity of the protein. Phylogenetic analysis of the deduced amino acid sequences of G. orientalis GST gene with other insect GST sequences revealed that the G. orientalis GST gene belongs to class I GST, forming a strong monophyletic group (100% bootstrap value) exclusively for class I GSTs from a diverse insect species. Northern blot analysis confirmed midgut-specific expression at transcriptional level, evidencing the midgut as a site for GST synthesis.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.04a
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• pp.67-67
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• 2003

The NAD$^{+}$-dependent 15-hydroxyprostagladin dehydrogenase (15-PGDH) is a key catabolic enzyme responsible for the control of the biological activities of prostagladins. So far the gene has been found in a diverse organism including three insect dipteran species and one lepidopteran species. In this study, a cDNA encoding the 15-PGDH gene homologue was isolated from the cDNA library of the mole cricket, Gryllotalpa orientalis. (omitted)d)

• Korean journal of applied entomology
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• v.31 no.4
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• pp.379-385
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• 1992

Ginseng damage by the African mole cricket (GTyllotalpa africana Palisot de Beauvois) was investigated in the field and laboratory from 1984 to 1991. Ginseng damage by G. africana occurred mainly in the 2nd year ginseng fields during May and June (spring period), and the damage was not nearly recognized in September and October (fall period) when densities of G. africana adults were higher in the field. In the laboratory and field cage, damage of 2nd year ginseng considerably decreased during fall period, which had no relation to ginseng diameter, and 3rd year ginseng was not damaged at all. Soil hardness seemed to influence on ginseng damage by G. africana adults.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.1
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• pp.37-44
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• 2003

Alcohol dehydrogenases (AHDs) are enzymes responsible for the catalysis of the reversible conversion of various alcohols to their corresponding aldehydes and ketonesis. Until now cDNA sequences of ADH gene is informed exclusively from several diptean species. We describe here the cDNA sequence and mRNA expression of a putative ADH gene from the mole cricket, Gryllotalpa orientalis, and phylogenetic relationships among known insect ADHs. The G. orientalis ADH cDNA sequences comprised of 798 bp encoding 266 amino acid residues. The multiple sequence alignment of G. orientalis ADH gene and known dipteran ADHs shared 100％ identity in the nine amino acid residues that are important for the enzymatic activity in Drosophila melanogaster. Percent sequence identity ranged from 25％ to 32％ among all insect ADHs including both types of ADHs. G. orientalis ADH gene showed no clear resemblance to any dipteran species and type. Phylogenetic analysis of the deduced amino acid sequences of G. orientalis ADH gene with available dipteran ADH genes including both types of ADHs further confirmed that the G. orientalis ADH gene is not clearly assigned to either type of ADHs. Northern blot analysis revealed a stronger signal in the fat body than midgut and epidermis, indicating that the fat body possibly is a main site for the synthesis of the G. orientalis ADH protein.