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Molecular Cloning of the Myosin Light Chain-2 cDNA of Gryllotalpa orientalis  

Cha, So Young (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Hwang, Jae Sam (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Kim, Iksoo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.9, no.1, 2004 , pp. 127-130 More about this Journal
Abstract
We describe here the cloning and characterization of a cDNA encoding a putative myosin light chain-2 (MLC-2) from the mole cricket, Gryllotalpa orientalis. The G. orientalis MLC-2 cDNA sequences comprised of 615 bp with 205 amino acid residues with a calculated molecular weight of approximately 23 kDa. The deduced protein sequence of G. orientalis MLC-2 cDNA showed 64% and 54% identity to Drosophila melanogaster MLC-2 and D. yakuba MLC-2, respectively. Northern blot analysis confirmed the muscle-specific expression of G. orientalis MLC-2.
Keywords
Insect; Oriental mole cricket; Gryllotalpa orientalis; cDNA sequences; Muscle; Myosin light chain-2;
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