• 패션비즈니스
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• 제15권5호
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• pp.178-194
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• 2011

Due to the population growth of the old aged, Korea is also entering into an aging society. But a research on the pattern design to the old aged is much to be desired. The purpose of this research is to design skirt pattern fitted for each body type by categorizing the lower body type of the old aged woman. For the way of research, categorized the body type by using the female measuring size from the 65 years old to 99 years old which has been measured by the Korean body size research as an assay data, and base on this data, a suitable skirt pattern for each body type of the old aged woman was designed by practicing the exterior evaluation. The data analysis was done with the statistical treatment in SPSS 12.0, and the results are as follows. The lower body type of the old aged woman were divided into the type 1. The circumference article is the biggest compared to the height article regarding to the lower body type of the old aged woman. The type 2. The height article and the circumference and thickness articles are the poorest. The type 3. The height article is the biggest and the hip width and calf circumference are thicker than the waist width. The type 4. The height article is the lowest and the waist width is thicker instead the hip and leg are thinner. In type 1, The pattern was modified by increasing 1cm a length of the dart and raising up 1.2cm at the cross point between side line and waist line. In type 2, the basic pattern was used, and in type 3, using the outline of the basic pattern however, increased 1cm a length of the dart and modified the amounts of dart by handling the art in curve, and modified the waist line to be more three-dimensional curves by raising up 1 cm at the cross point between waist line and side line and falling down 0.5cm at the cross point between the back center line and the waist line. In type 4, modified the amounts of front and back dart to 1.25cm, and raised up 0.8cm at the cross point between side line and waist line, and modified the waist line and the side seam line in a gentle curve by reducing circumference of the skirt tail to 1cm from the side seam line.

• 한국양식학회지
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• 제8권1호
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• pp.1-19
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• 1995

난황형성 중인 암 참돔의 혈청 내에는 암 특이혈청단백질이 존재하고 있음이 Ouchterlony의 면역확산 검정과 면역 전기영동에 의하여 밝혀졌으며, 이러한 암 특이혈청단백질은 암 $\cdot$수 혈청과 난추출액의 면역학적인 조사에 의해 난황단백 전구체임이 밝혀졌다. 또한 난추출액으로부터 정제된 난황단백질은 난황전구체와 공통의 항원성이 있음도 면역학적인 조사에 의하여 알 수 있었다. 변형한 효소면역측정법의 타당성을 조사하였다. 일정 비율로 희석한 성숙중인 암 혈청의 흡광도 곡선은 난황단백질의 표준곡선과 평행하였다 본 효소면역측정법의 평균 회수율은 $109\pm5.6\%$이었으며, $10\%$ 이내의 assay내 변동계수 범위는 $31\~1,000ng/ml$이었다. 생식소의 성숙 단계에 대한 혈장내의 난황단백전구체의 양과의 관계를 조사하기 위하여 휴지기후기(1월)부터 난황형성기(4월)까지 난황단백질에 대한 항체를 이용한 효소면역측정법에 의하여 혈장 난황단백전구체의 양을 측정하였다. 난황단백전구체의 양은 난황형성 전기인 2월부터 증가하기 시작하여, 난황형성기(3월부터 4월) 동안에는 난이 성장하면서 계속 증가하였다 2월부터 혈장내의 난황단백전구체의 양이 암$\cdot$수에 따라 현저하게 차이가 났다. 따라서 참돔 친어의 성 구분은 효소면역 측정법에 의하여 2월부터는 명확히 할 수 있다

• Biomolecules & Therapeutics
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• 제30권6호
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• pp.553-561
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• 2022

Chronic myeloid leukemia (CML) is a slowly progressing hematopoietic cell disorder. Sphingosine kinase 1 (SPHK1) plays established roles in tumor initiation, progression, and chemotherapy resistance in a wide range of cancers, including leukemia. However, small-molecule inhibitors targeting SPHK1 in CML still need to be developed. This study revealed the role of SPHK1 in CML and investigated the potential anti-leukemic activity of hirsuteine (HST), an indole alkaloid obtained from the oriental plant Uncaria rhynchophylla, in CML cells. These results suggest that SPHK1 is highly expressed in CML cells and that overexpression of SPHK1 represents poor clinical outcomes in CML patients. HST exposure led to G2/M phase arrest, cellular apoptosis, and downregulation of Cyclin B1 and CDC2 and cleavage of Caspase 3 and PARP in CML cells. HST shifted sphingolipid rheostat from sphingosine 1-phosphate (S1P) towards the ceramide coupled with a marked inhibition of SPHK1. Mechanistically, HST significantly blocked SPHK1/S1P/S1PR1 and BCR-ABL/PI3K/Akt pathways. In addition, HST can be docked with residues of SPHK1 and shifts the SPHK1 melting curve, indicating the potential protein-ligand interactions between SPHK1 and HST in both CML cells. SPHK1 overexpression impaired apoptosis and proliferation of CML cells induced by HST alone. These results suggest that HST, which may serve as a novel and specific SPHK1 inhibitor, exerts anti-leukemic activity by inhibiting the SPHK1/S1P/S1PR1 and BCR-ABL/PI3K/Akt pathways in CML cells, thus conferring HST as a promising anti-leukemic drug for CML therapy in the future.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제28권2호
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• pp.95-110
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• 2006

The treatment for oral and maxillofacial carcinoma with chemotherapeutic agents is evaluated by many effective methods to reduce the tumor mass and cancer cell proliferation. However these chemotherapy have many serious side effects, such as bone marrow suppression, renal toxicity, G-I troubles. Therefore a possible approach to develop a clinically applicable chemotherapeutic agent is to screen anticancer activity of Taxol which is known to have very little side effect and have been used to breast cancer and ovarian carcinoma. Taxol is a new anti-microtubular anti-cancer agent extracted from the bark of the Pacific yew, Taxus brevifolia. Paclitaxel(Taxol) acts by promoting tubulin polymerization and over stabilizing microtubules agianst depolymerization. Despite the constant improvements of methods of the cancer treatment especially chemotherapy, the rate of cancer metastasis and recurrent are not decreased. Thus the investigation of new drug which have very little side effect and a possible clinically application continues to be a high priority. Considering that the Taxol have shown very effective chemotherapeutic agent with relatively low toxicity in many solid tumors, it deserves to evaluate its efficacy in oral squamous cell carcinoma. In this study, to investigate the in-vivo and in-vitro anti-cancer efficacy of Taxol in oral squamous cell carcinoma and lastly, the potency of Paclitaxel in the clinical application for oral cancer was evaluated. In vivo study, after HN22 cell line were xenografted in nude mice, the growth of tumor mass was observed, 3 mg/Kg taxol was injected intraperitoneally into nude mice containing tumor mass. The methods of these study were measurement of total volume of tumor mass, histopathologic study, immunohistochemical study, drug resistance assay, growth curve, MTT assay, flow cytometry, cDNA microarray in vivo and in vitro. The results were obtained as following. 1. The visual inspection of the experimental group showed that the volume of the tumor mass was slightly decreased but no significant difference with control group. 2. Ki-67 index was decreased at weeks 4 in experimental group. 3. Microscopic view of the xenografted tumor mass showed well differentiated squamous cell carcinoma and after Taxol injection, some necrotic tissue was seen weeks 4. 4. The growth curve of the tumor cells were decreased after 1day Taxol treatment. 5. According to the MTT assay, HN22 cell line showed relative drug resistancy above $5\;{\mu}g/ml$ concentrations of Taxol. 6. In drug resistance assay, the decrease of cell counts was seen relatively according to concentration. 7. In Flow cytometry, G2M phase cell arrests were seen in low concentration of the Taxol, while S phase cell arrests were seen in high concentration of the Taxol. 8. Using cDNA microarray technique, variable gene expression of ANGPTL4, TXNRD1, FAS, RRAGA, CTGF, CYCLINEA, P19, DUSP5, CEBPG, BTG1 were detacted in the oral squamous cell carcinoma cell after taxol treatment. In this study paclitaxel is effective against oral squamous cell carcinoma cell lines in vitro, but week effect was observed in vivo. So we need continuous study about anticancer effect of taxol in vivo in oral squamous cell carcinoma.

• 공업화학
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• 제26권5호
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• pp.569-574
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• 2015

미세조류 Arthrospira platensis (A. platensis)는 색소(chlorophyll, carotenoids), 지질을 다량 함유하여 기능성 식품 생산에 중요한 자원으로 평가받고 있다. A. platensis에 조사선량 240 kGy의 전자빔을 조사하여 균주를 개량하고자 하였으며, 스크리닝을 통해 지질 함량이 높은 EM24를 선별하였다. EM24는 세포생장률 및 색소농도가 야생균주에 비해 약 1.2배 증가함을 확인하였다. 또한 형광강도법으로 측정 후 triolin을 이용해 세포 내 지질 농도를 정량하고자 하였다. EM24의 지질 농도는 62.9 mg/L로 야생균주(38.9 mg/L)에 비해 약 1.5배 증대되었다. 세포 내 지방산 또한 gamma-linoleic acid(GLA) 함량이 약 1.5배 증가하였다.

• 한국동물위생학회지
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• 제14권2호
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• pp.110-120
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• 1991

28 porcine enteroviruses were isolated from 86 pig-feces of 9 swine farms located in south region, Chung-buk, from March to September 1990. Physico-chemical properties and pathogenicity of isolates were investigated. Results obtained throughout experiments are summarized as follows. According to the age, weanlings(40-90 days), sucklings(10-30 days) and adult pigs(6 months over) showed the isolation rate of 67%. 8% and 4%, respectively. By physico-chemical tests, YD-90/22, YD-90/43 and YD-90/64 strains were found to be ether, chloroform and PH stable. Nucleic acid test suggests the virus to have a DNA genome. Most of the Isolates were not evident of hemagglutinin using erythrocytes from various mammalian & avian. 22 strains among the isolates were shown CPE type I and the remainders were CPE type II. 3 strains among isolates of CPE type I strains were neutralized with high titers to serotype 2 antiserum. In the study on virus growth curve in PK-l5 cells, YD-90/22, YD-90/43 and YD-90/64 strains showed the maximum infectivity titers($10^{6.0}-l0^{6.5} TCID({50}ml$) at 4days post inoculation(PI). When 30 day-old commercial piglets were inoculated only intraoral route with the YD-90/22 strain at $10^{6.0} TCID_{50}ml,$ piglets not showed the symptoms. But piglets inoculated by intramuscle route, intraoral and intramuscle route after pretreat with dexamethasone(2.5mg /kg) for 5 days were shown the symptoms of anorexia, diarrhea, pyrexia and ataxia at 4th-6th days PI. The viral reisolation in the virus-inoculated piglets was examined from feces. The viruses were recovered intermittently from 2nd to 16th day PI and at 4th-6th day PI, all piglets excreted viruses.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.75-82
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• 2008

Functional stay-green is a beneficial trait that may increase grain yield through the sustained photosynthetic competence during monocarpic senescence in cereal crops. The temporal changes of photosynthesis and related characteristics throughout the grain filling period of a stay-green japonica rice "SNU-SG1" was compared in growth chamber conditions with three high-yielding cultivars(HYVs) and their $F_1$ hybrids with SNU-SG1. SNU-SG1 exhibited a typical characteristic of functional stay-green in terms of chlorophyll degradation and photosynthetic competence during grain filling. According to the photosynthesis-light response curve measured at 10 and 35 d after heading for the flag leaf, SNU-SG1 exhibited higher initial light conversion efficiency and thus higher gross photosynthetic rate at light saturation compared to HYVs. Light saturation point was not different among genotypes, ranging from 1000 to 1500 ${\mu}mol$ photon $m^{-2}s^{-1}$. Net photosynthetic rate at light saturation($P_{max}$) of the upper four leaves in SNU-SG1 was much higher and sustained longer throughout grain-filling than HYVs and $F_1$ hybrids. The sustained high photosynthetic competence of SNU-SG1 during grain filling was ascribed to the longer maintenance of high mesophyll conductance that resulted from not only high chlorophyll content and its delayed degradation but also the slow degeneration of photosystem II(PS II) as judged by chlorophyll fluorescence($F_v/F_m$) of flag leaves. $F_1$ hybrids showed slow degeneration of photosystem II similar to the male parent SNU-SG1 while chlorophyll degradation pattern close to female parents, thus exhibiting a little higher $P_{max}$ than female parents. These results suggest that SNU-SG1 has a typical functional stay-green trait that can be utilized for increasing rice yield potential through the improved dry matter production during grain filling.

• 보건행정학회지
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• 제18권4호
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• pp.85-110
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• 2008

In this study, we explore the long-term demand forecasting of high-price medical equipments based on logistic and Bass diffusion model. We analyze the specific pattern of each equipment's diffusion curve by interpreting the parameter estimates of Bass diffusion model. Our findings are as follows. First, ultrasonic imaging system, CT are in the stage of maturity and so, the future demands of them are not too large. Second, medical image processing unit is between growth stage and maturity stage and so, the demand is expected to increase considerably for two or three years. Third, MRI is in the stage of take-off and Mammmography X-ray system is in the stage of maturity but, estimates of the potential number of adopters based on logistic model is considerably different to that based on Bass diffusion model. It means that additional data for these two equipments should be collected and analyzed to obtain the reliable estimates of their demands. Fourth, medical image processing unit have the largest q value. It means that the word-of-mouth effect is important in the diffusion of this equipment. Fifth, for MRI and Ultrasonic system, q/p values have the relatively large value. It means that collective power has an important role in adopting these two equipments.

• 한국진공학회지
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• 제9권4호
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• pp.406-410
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• 2000

Hot-wall epitaxy 방법으로 GaAs (100) 기판 위에 9 $\mu\textrm{m}$의 CdTe (111)을 완충층으로 성장하고 그 위에 in-situ로 $Hg_{1-x}Cd_x$/Te (MCT)박막을 성장하였다. 성장된 MCT박막의 2결정 x-선 요동곡선의 반치폭 값은 125 arcsec이었으며 표면 형상의 roughness는 10 nm의 작고 깨끗한 면을 나타내었다. 성장된 MCT 박막에 대한 광전류 측정으로부터 최대 peak 파장과 cut off 파장은 각각 1.1050 $\mu\textrm{m}$ (1.1220 eV)와 1.2632 $\mu\textrm{m}$ (0.9815 eV)임을 알았다 이 peak 파장은 광전도체의 intrinsic transition에 기인한 band gap에 대응하는 봉우리이다. 이로부터 MCT 박막은 1.0 $\mu\textrm{m}$에서 1.6 $\mu\textrm{m}$의 근적외선 파장 영역을 감지할 수 있는 광전도체용 검출기로 쓰일 수 있음을 알았다.

• Applied Biological Chemistry
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• 제34권1호
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• pp.67-74
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• 1991

내당 내알콜성 균주인 S. cervisiae D1을 이용하여 알콜 발효를 할 때 ethanol 생산성을 증대시키기 위하여 발효시 $Ca^{2+}$을 첨가하여 발효에 미치는 영향에 관해서 연구한 결과 다음의 결론을 얻었다. 초기 glucose 농도를 400g/l로 하였을 때 $Ca^{2+}$에 의해 ethanol 생산성이 증가하였고 생균수도 증가하였으며 최적 첨가 농도는 0.8 mM이었다. 초기 glucose농도를 달리하고 발효하였을 때는 $Ca^{2+}$의 영향이 초기 glucose 농도가 높을수록 켰다. Ethanol 첨가에 의해 비증식 속도가 감소하였지만 $Ca^{2+}$을 0.8 mM 첨가시 비증식 속도의 저해가 감소되었다. 또한 ethanol을 첨가하지 많았을 때는 $Ca^{2+}$을 첨가한 경우와 첨가하지 않은 경우의 비증식 속도가 같았다. Ethanol 첨가 농도에 의해 사멸 속도가 증가하였으나 $Ca^{2+}$을 0.8 mM 첨가시 비사멸 속도가 감소하였다. Acidification curve에서도 ethanol 첨가시 final pH가 증가하였으나 $Ca^{2+}$을 0.8 mM 첨가하면 final pH가 감소하였다. 따라서 $Ca^{2+}$ 첨가시 ethanol에 대한 내성이 더 증가된다는 것을 알 수 있었다.