• Biomolecules & Therapeutics
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• 제23권2호
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• pp.174-179
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• 2015

BP201, porcine lung tissue-derived phospholipids, consists of phosphatidylcholine as a major phospholipid species. BP201 promoted hair growth after application onto the shaved backs of BALB/c and C3H mice. Its effect was enhanced when applied together with minoxidil (MNX) in C3H mice. When the tissue specimens prepared from the shaved skins of BP201-treated and control mice were microscopically examined, the total numbers of hair follicles in both anagen and telogen phases of BP201-treated mice were significantly higher than those of control mice. The numbers of hair follicles in the anagen phase of BP201-treated mice were also higher than those of control mice. In combination with MNX, BP201 further increased the total number of hair follicles, but did not alter the percentage of hair follicles in the anagenic phase. BP201 also increased the proliferation of human hair follicle dermal papilla cells. Collectively, BP201 possesses hair growth promoting potential, which would suggest its use singly or in combination for hair growth products.

• 대한한방부인과학회지
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• 제18권3호
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• pp.77-91
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• 2005

Purpose : Trichosanthis Radix is traditional medical herb which has been shown to inhibit tumor cell proliferation. In this study, the effects of Trichosanthis Radix extract were investigated on inducing growth inhibition and apoptosis of human uterine cervical carcinoma cells. Methods : Human uterine cervical carcinoma cells line, ME-180, was used for the study. The cells were treated with varying concentrations of Trichosanthis Radix extract. Cell growth and inhibitory rate were measured by MTT assay. Apoptosis induction was detected by fluorescence microscopy, DNA ladder formation and flow cytometry. Results : Trichosanthis Radix extract inhibited the growth of human uterine cervical carcinoma cells in a dose-dependent manner. It induced ME-180 cells to undergo apoptosis including fragmented nuclei and nucleosome-sized DNA fragmentation. Flow cytometric analysis showed the increasing rate of apoptotic cells by Trichosanthis Radix extract. Reduction of mitochondrial membrane potential and increase in caspase-3 activity and were found in ME-180 cells treated with Trichosanthis Radix extract. Conclusion : Our data suggest that Trichosanthis Radix extract inhibit the growth and proliferation of ME-180 cells by apoptotic induction and facilitates its activity via caspase-3 activation initiated by depolarization of mitochondria.

• Fisheries and Aquatic Sciences
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• 제5권2호
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• pp.108-113
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• 2002

Apical and subapical fragments of Gracilaria verrucosa (Hudson) Papenfuss (Rhodophyta) were cultured on a temperature-light gradient table with four temperature $(15,\;20,\;25\;and\;30^{\circ}C)$ and five light intensity $(20,\;40,\;60,\;80\; and\;100{\mu}mol\;photons\;m^{-2}s^{-l})$ regimes to examine growth responses. Their growth was measured weekly. Plant weight and lateral branch formation were affected by temperature and light intensity. As compared with other reports, relative growth rate (RGR) in both fragments was more or less high with 6.27 to $11.95\%$ $day^{-1}$. It was lowest at $15^{\circ}C$ with $20\mu mol$ photons $m^{-2}s^{-l}$, whereas, the highest value was recorded at $25^{\circ}C$ with $100\mu mol$ photons $m^{-2}s^{-l}$ During the experimental period, the growth pattern in G. verrucosa was significantly different between apical and subapical fragments, even though RGR based on weight was similar. Growth in apical fragments depended on elongation by apical growth. By contrast, subapical fragments mainly increased their weights by proliferation of lateral branch. This suggests that intercalary activity (e.g. lateral branch formation) is also an important means for growth of the thallus in Korean G. verrucosa. In conclusion, the relatively high growth potential with the intercalary activity in G. verrucosa will be helpful for mariculture in Korea.

• Journal of Microbiology and Biotechnology
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• 제25권4호
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• pp.431-438
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• 2015

Ever since the ban on antibiotic growth promoters (AGPs), the livestock death rate has increased owing to pathogenic bacterial infections. There is a need of developing AGP alternatives; however, the mechanisms by which AGP enhances livestock growth performance are not clearly understood. In this study, we fed 3-week-old swine for 9 weeks with and without AGPs containing chlortetracycline, sulfathiazole, and penicillin to investigate the effects of AGPs on swine gut microbiota. Microbial community analysis was done based on bacterial 16S rRNA genes using MiSeq. The use of AGP showed no growth promoting effect, but inhibited the growth of potential pathogens during the early growth stage. Our results showed the significant increase in species richness after the stabilization of gut microbiota during the post-weaning period (4-week-old). Moreover, the swine gut microbiota was divided into four clusters based on the distribution of operational taxonomic units, which was significantly correlated to the swine weight regardless of AGP treatments. Taxonomic abundance analysis indicated a negative correlation between host weight and the abundance of the family Prevotellaceae species, but showed positive correlation to the abundance of the family Spirochaetaceae, Clostridiaceae_1, and Peptostreptococcaeae species. Although no growth performance enhancement was observed, the use of AGP inhibited the potential pathogens in the early growth stage of swine. In addition, our results indicated the ecological succession of swine gut microbiota according to swine weight. Here, we present a characterization of swine gut microbiota with respect to the effects of AGPs on growth performance.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1361-1368
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• 2007

Endophytic bacteria associated with the roots of coastal sand dune plants were isolated, taxonomically characterized, and tested for their plant growth-promoting activities. Ninety-one endophytic bacterial isolates were collected and assigned to 17 different genera of 6 major bacterial phyla based on partial 16S rDNA sequence analyses. Gammaproteobacteria represented the majority of the isolates (65.9%), and members of Pseudomonas constituted 49.5% of the total isolates. When testing for antagonism towards plant pathogenic fungi, 25 strains were antagonistic towards Rhizoctonia solani, 57 strains were antagonistic towards Pythium ultimum, 53 strains were antagonistic towards Fusarium oxysporum, and 41 strains were antagonistic towards Botrytis cinerea. Seven strains were shown to produce indole acetic acid (IAA), 33 to produce siderophores, 23 to produce protease, 37 to produce pectinase, and 38 to produce chitinase. The broadest spectra of activities were observed among the Pseudomonas strains, indicating outstanding plant growth-promoting potential. The isolates from C. kobomugi and M. sibirica also exhibited good plant growth-promoting potential. The correlations among individual plant growth-promoting activities were examined using phi coefficients, and the resulting data indicated that the production of protease, pectinase, chitinase, and siderophores was highly related.

• Weed & Turfgrass Science
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• 제4권4호
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• pp.321-329
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• 2015

The present study aimed to evaluate the phytotoxic potential of essential oils. For this purpose, 18 essential oil samples extracted from Korean plants and 64 commercial essential oils were screened for their phytotoxic potential against the seedling growth of Brassica napus L. (rapeseed). Among the 82 samples, 11 commercial oils (cinnamon, citronella, clove, cumin seed, geranium, jasmine, lemongrass, palmarosa, pimento, rose otto and spearmint) strongly inhibited the seedling growth with $GR_{50}$ value < $150{\mu}g\;mL^{-1}$. Major components from these effective essential oils were identified by solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS). GC-MS analyses revealed that the effective samples mainly consist of benzyl benzoate, carvone, citral, citronellol, eugenol, geraniol, D-limonene and terpinene. Subsequently, bioactivity of these individual components was evaluated against the seedling growth of B. napus, Echinochloa crus-galli and Aeschynomene indica. The components from different chemical groups exhibited different potency in inhibiting the seedling growth with varied $GR_{50}$ values ranged from $29{\mu}g\;mL^{-1}$ to > $1000{\mu}g\;mL^{-1}$. In the greenhouse experiment, citral and geraniol completely suppressed the growth of all the tested 10 plants at $100kg\;ha^{-1}$. In conclusion, the individual essential oil components geraniol and citral could be used as natural herbicides for weed management.

• 품질경영학회지
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• 제50권3호
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• pp.349-371
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• 2022

Purpose: Compared to the rapid growth rate of the domestic automotive LED industry so far, the predictive analysis method for demand forecasting or market outlook was insufficient. Accordingly, product characteristics are analyzed through the life trend of LEDs for automotive exterior lamps and the relative strengths of p and q using the Bass model. Also, future demands are predicted. Methods: We used sales data of a leading company in domestic market of automotive LEDs. Considering the autocorrelation error term of this data, parameters m, p, and q were estimated through the modified estimation method of OLS and the NLS(Nonlinear Least Squares) method, and the optimal method was selected by comparing prediction error performance such as RMSE. Future annual demands and cumulative demands were predicted through the growth curve obtained from Bass-NLS model. In addition, various nonlinear growth curve models were applied to the data to compare the Bass-NLS model with potential market demand, and an optimal model was derived. Results: From the analysis, the parameter estimation results by Bass-NLS obtained m=1338.13, p=0.0026, q=0.3003. If the current trend continues, domestic automotive LED market is predicted to reach its maximum peak in 2021 and the maximum demand is $102.23M. Potential market demand was $1338.13M. In the nonlinear growth curve model analysis, the Gompertz model was selected as the optimal model, and the potential market size was $2864.018M. Conclusion: It is expected that the Bass-NLS method will be applied to LED sales data for automotive to find out the characteristics of the relative strength of q/p of products and to be used to predict current demand and future cumulative demand.

• Mycobiology
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• 제33권1호
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• pp.19-22
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• 2005

We investigated the effect of nitrogen fixing Azotobacter bioinoculant on the mycelial growth and the rate of substrate utilization by Pleurotus eous. The synergistic or antagonistic role of the microorganism during dual culturing with the mushroom or the competitor molds Trichoderma viride, and Trichoderma reesi was studied. Azotobacter was inhibitory to the molds, which are competitive to the mushroom in the seed spawn substrate, but was synergistic towards the mushroom. The growth, substrate utilization potential as total nitrogen content and cellulase enzyme activities of the mushroom in the seed spawn substrate were also enhanced in the presence of the bioinoculant at lower inoculum concentrations, upto 5 ml broth culture per spawn bottle.

• 한국미생물·생명공학회지
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• 제27권5호
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• pp.415-418
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• 1999

Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 1999년도 춘계학술대회 논문집
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• pp.263-268
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• 1999

With HAZ of A 106 Gr B steel weldment, fatigue test in air, electrochemical polarization test and corrosion fatigue test in 3.5wt.% NaCl solution were performed changing load ratio. Obtained results are as follows. 1) K$\sub$op/ was independent of K$\sub$max/ and load ratio in fatigue crack growth. 2) In variation of load ratio, the scatter band of crack growth curve was reduced by half considering crack closure 3) In the result of electrochemical polarization test, current density was increased abruptly when potential was larger than corrosion potential. 4) Fatigue crack growth rate in corrosive environment was markly higher than the rate in air because of corrosion characteristics of the material and anodization of inner surface crack.