• Journal of Microbiology and Biotechnology
• /
• 제31권3호
• /
• pp.398-407
• /
• 2021

Using salt-tolerant bacteria to protect plants from salt stress is a promising microbiological treatment strategy for saline-alkali soil improvement. Here, we conducted research on the growth-promoting effect of Brevibacterium frigoritolerans on wheat under salt stress, which has rarely been addressed before. The synergistic effect of B. frigoritolerans combined with representative salt-tolerant bacteria Bacillus velezensis and Bacillus thuringiensis to promote the development of wheat under salt stress was also further studied. Our approach involved two steps: investigation of the plant growth-promoting traits of each strain at six salt stress levels (0, 2, 4, 6, 8, and 10%); examination of the effects of the strains (single or in combination) inoculated on wheat in different salt stress conditions (0, 50, 100, 200, 300, and 400 mM). The experiment of plant growth-promoting traits indicated that among three strains, B. frigoritolerans had the most potential for promoting wheat parameters. In single-strain inoculation, B. frigoritolerans showed the best performance of plant growth promotion. Moreover, a pot experiment proved that the plant growth-promoting potential of co-inoculation with three strains on wheat is better than single-strain inoculation under salt stress condition. Up to now, this is the first report suggesting that B. frigoritolerans has the potential to promote wheat growth under salt stress, especially combined with B. velezensis and B. thuringiensis.

• 한국농림기상학회지
• /
• 제18권3호
• /
• pp.109-119
• /
• 2016

본 연구는 우리나라의 대표 활엽수종 중의 하나인 신갈나무를 대상으로 기후변화에 의한 연도별 생장도일의 변화가 잠재분포 및 생장에 미치는 영향을 평가하기 위해 수행하였다. 이를 위해 신갈나무 분포 지역의 기후특성을 반영한 군집분석을 실시하였으며, 그 결과 7개의 기후군집으로 분류되었다. 각 군집에 포함된 시군에서 수집된 일평균기온 자료를 기반으로 1951년부터 2010년까지 60년 동안의 연도별 생장도일과 기온효과지수를 산출함으로써 시간 경과에 따른 신갈나무의 잠재분포 범위와 생장의 변화 추이를 평가하였다. 이와 함께 기후변화 시나리오 RCP 4.5와 RCP 8.5를 적용하여 2011년부터 2100년까지 연도별 생장도일과 온도효과지수를 산출하여, 기후변화에 의한 신갈나무의 잠재분포 범위와 생장의 변화를 예측하였다. 신갈나무가 현재 분포하고 있는 지역의 생장도일을 연도별로 산출하여 비교한 결과 위도와 해발고도가 낮은 지역은 시간이 경과함에 따라 신갈나무에 적합한 생육가능 범위를 벗어나는 것으로 분석되었다. 특히 기후변화 시나리오를 적용할 경우 지속적인 기온의 상승으로 인해 2050년 이후에는 잠재분포의 변화뿐만 아니라 생장에도 큰 지장을 주는 것으로 평가되었다.

• 생태와환경
• /
• 제48권4호
• /
• pp.287-295
• /
• 2015

본 연구는 2014년 9월과 2015년 3월에 채수한 팔당호 3지점의 현장수 (PD-1, PD-2 및 PD-3)를 이용하여 북한강에서 우점하는 A. circinalis의 휴면포자 발아 및 성장 잠재력을 평가하였다. 질소와 인 농도는 팔당댐앞 (PD-1)과 광동교 (PD-3)에 비교하여 금남리 (PD-2)에서 좀 더 높게 나타났다. 팔당호 3지점의 총질소에 대한 영양상태지수 (TSI)는 65~85의 범위로 나타났으며, 부영양 또는 과영양 상태로 평가되었다. 또한 총인에 대한 영양상태지수(TSI) 값은 49~68의 범위로서, 중영양 또는 부영양 상태로 평가되었다. 휴면포자 발아 잠재력은 영양염 농도가 높은 금남리 지점 (PD-2)에서 좀 더 높게 나타났으며, 영양세포 성장 잠재력도 영양염 농도가 높은 현장수에서 증가하였다.

• 대한기계학회논문집A
• /
• 제20권11호
• /
• pp.3415-3423
• /
• 1996

The compact tension specimen geometry has been widely used for measuring fatigue crack growth rates at elevated temperature when the fatigue load is under tension/tension condition. However, most of the elevated temperature components which have significant crack growth life experience fatigue load under tension/compression conditions. Thus test techniques are required since the compact tension specimen cannot be used for tension/compression loading. In this paper, a simplified test procedure for measureing fatigue crack growth rates is proposed, which employs a round bar specimen with a small surface crack. Fatigue crack growth rates under tension/ tension loading conditions at elevated temperature were measured according to the proposed procedure and compared with those previously measured by C/(T) specimens. Since both the measured crack growth rates were comparable, the fatigue crack growth rates under tension/ compression load can be reliably measured by the proposed procedure. For monitoring crack depth. DC electric potential method is employed and an optimal probe location and current input conditions were proposed.

• Journal of Microbiology and Biotechnology
• /
• 제16권3호
• /
• pp.399-407
• /
• 2006

In this study, an electrical conductive carbon fiber was used as a biofilter matrix to electrochemically improve the biofilter function. A bioreactor system was composed of carbon fiber (anode), titanium ring, porcelain ring, inorganic nutrient reservoir, and VOC reservoir. Electric DC power of 1.5 volt was charged to the carbon fiber anode (CFA) to induce the electrochemical oxidation potential on the biofilter matrix, but not to the carbon fiber (CF). We tested the effects of electrochemical oxidation potential charged to the CFA on the biofilm structure, the bacterial growth, and the activity for metabolic oxidation of VOCs to $CO_2$, According to the SEM image, the biofilm structure developed in the CFA appeared to be greatly different from that in the CF. The bacterial growth, VOCs degradation, and metabolic oxidation of VOCs to $CO_2$ in the CFA were more activated than those in the CF. On the basis of these results, we propose that the biofilm structure can be improved, and the bacterial growth and the bacterial oxidation activity of VOCs can be activated by the electrochemical oxidation potential charged to a biofilter matrix.

• Journal of Microbiology and Biotechnology
• /
• 제23권11호
• /
• pp.1544-1553
• /
• 2013

Despite the importance of acetate kinase in the metabolism of bacteria, limited structural studies have been carried out on this enzyme. In this study, a three-dimensional structure of the Escherichia coli acetate kinase was constructed by use of molecular modeling methods. In the next stage, by considering the structure of the catalytic intermediate, trifluoroethanol (TFE) and trifluoroethyl butyrate were proposed as potential inhibitors of the enzyme. The putative binding mode of these compounds was studied with the use of a docking program, which revealed that they can fit well into the enzyme. To study the role of these potential enzyme inhibitors in the metabolic pathway of E. coli, their effects on the growth of this bacterium were studied. The results showed that growth was considerably reduced in the presence of these inhibitors. Changes in the profile of the metabolic products were studied by proton nuclear magnetic resonance spectroscopy. Remarkable changes were observed in the quantity of acetate, but other products were less altered. In this study, inhibition of growth by the two inhibitors as reflected by a change in the metabolism of E. coli suggests the potential use of these compounds (particularly TFE) as bacteriostatic agents.

• 대한약침학회지
• /
• 제15권3호
• /
• pp.31-38
• /
• 2012

Sophorae Radix (SR) plays a role in a number of physiologic and pharmacologic functions in many organs. Objective: The aim of this study was to clarify the potential role for transient receptor potential melastatin 7 (TRPM7) channels in SR-inhibited growth and survival of AGS and MCF-7 cells, the most common human gastric and breast adenocarcinoma cell lines. Methods: The AGS and the MCF-7 cells were treated with varying concentrations of SR. Analyses of the caspase-3 and - 9 activity, the mitochondrial depolarization and the poly (ADPribose) polymerase (PARP) cleavage were conducted to determine if AGS and MCF-7 cell death occured by apoptosis. TRPM7 channel blockers ($Gd^{3+}$ or 2-APB) and small interfering RNA (siRNA) were used in this study to confirm the role of TRPM7 channels. Furthermore, TRPM7 channels were overexpressed in human embryonic kidney (HEK) 293 cells to identify the role of TRPM7 channels in AGS and MCF-7 cell growth and survival. Results: The addition of SR to a culture medium inhibited AGS and MCF-7 cell growth and survival. Experimental results showed that the caspase-3 and -9 activity, the mitochondrial depolarization, and the degree of PARP cleavage was increased. TRPM7 channel blockade, either by $Gd^{3+}$ or 2-APB or by suppressing TRPM7 expression with small interfering RNA, blocked the SR-induced inhibition of cell growth and survival. Furthermore, TRPM7 channel overexpression in HEK 293 cells exacerbated SR-induced cell death. Conclusions: These findings indicate that SR inhibits the growth and survival of gastric and breast cancer cells due to a blockade of the TRPM7 channel activity. Therefore, TRPM7 channels may play an important role in the survival of patients with gastric and breast cancer.

• 한국지리정보학회지
• /
• 제21권3호
• /
• pp.138-148
• /
• 2018

토지이용의 합리화를 위하여 도입된 토지이용규제는 사회경제적인 발전과 함께 복잡다기화 되면서 국민경제생활에 불편을 초래한다는 이유로 최근 규제 완화로 패러다임이 변화하고 있다. 이에 본 연구에서는 CA 모델을 이용하여 시뮬레이션 함으로써 부산권 도시성장의 잠재지역을 도출하고, 토지이용 규제지역과 함께 공간적 특성을 분석하였다. 분석을 통해 토지이용규제 제도가 실제로 도시성장을 억제하고 토지이용의 효율화를 도모하고 있는지, 또는 국민생활에 불편을 초래할 수 있는 다른 요인이 있는지를 검토하였다. 분석결과 도시외부의 개발압력이 높은 지역에 존재하는 개발제한구역은 토지이용규제라는 역할을 수행하고 있었지만, 많은 지역에서 토지이용에 대한 다중규제가 존재하는 것으로 나타났다. 따라서 순기능을 하고 있는 토지이용규제는 최대한 유지하면서, 도시성장 잠재력이 높은 지역에 존재하는 다중규제를 재고하는 등 다양한 접근과 검토가 필요할 것으로 사료된다.

• The Plant Pathology Journal
• /
• 제24권4호
• /
• pp.407-416
• /
• 2008

Studies were conducted to determine the effects of temperature, solute potential and carbon source on the mycelial growth, sclerotia development, and apothecium production of an isolate of Sclerotinia sclerotiorum. Mycelial growth rate was greatest at $25^{\circ}C$ on potato dextrose agar (PDA) medium amended with up to 2% NaCl (${\psi}s{\leq}1.91\;MPa$) and thereafter, growth rate declined. The least number of sclerotia were produced at $20^{\circ}C$on both PDA and malt extract agar (MEA) amended with 8% NaCl (${\psi}s=6.62\;MPa$). With increasing temperature and decreasing solute potential the number and size of sclerotia were significantly reduced. The combined effect of temperature, solute potential and carbon source on sclerotia production were highly significant and had an impact on the development of the rind layer cells of sclerotia. These cells lacked a transparent cell wall which was surrounded by a compact melanized layer, and some of these cells appeared to be devoid of cell contents or were totally vacuolated. The survival of the sclerotia with increase in salinity and temperature appeared to affect melanization and the nature of the rind cells. The observations of this study re-enforces the need for an integrated disease management to control S. sclerotiorum.

• 한국결정성장학회:학술대회논문집
• /
• 한국결정성장학회 1999년도 PROCEEDINGS OF 99 INTERNATIONAL CONFERENCE OF THE KACG AND 6TH KOREA·JAPAN EMG SYMPOSIUM (ELECTRONIC MATERIALS GROWTH SYMPOSIUM), HANYANG UNIVERSITY, SEOUL, 06월 09일 JUNE 1999
• /
• pp.45-49
• /
• 1999

When concentration of vacancies in a CZ silicon crystal is defined by molar fraction XB, the degree of super-saturation $\sigma$ is given by [XB-XBS]/XBS=XB/XBS-1=ln (XB/XBS) because XB/XBS is nearly equal to unity. Here, XBS is the saturated concentration of vacancies in a silicon crystal and XB is a little larger than XBS. According to Bragg-Williams approximation, the chemical potential of the vacancies in the crystal is given by ${\mu}$B=${\mu}$0+RT ln XB+RT ln ${\gamma}$, where R is the gas constant, T is temperature, ${\mu}$0 is an ideal chemical potential of the vacancies and ${\gamma}$ is an adjustable parameter similar to the activity of solute in a solution. Thus, $\sigma$(T) is equal to (${\mu}$B-${\mu}$BS)/RT. Driving force of nucleation of the vacancy agglomeration will be proportional to the chemical potential difference (${\mu}$B-${\mu}$BS) or $\sigma$(T), while growth of the vacancy agglomeration is proportional to diffusion of the vacancies and grad ${\mu}$B.