• 대한약침학회지
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• 제16권3호
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• pp.39-45
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• 2013

Objectives: Buxus Microphylla var. Koreana Nakai Extract (BMKNE) is used as a folk remedy for malaria and veneral disease. In the present study, we investigated the effects of BMKNE in the growth and the survival of AGS cells, the most common human gastric adenocarcinoma cell lines. Methods: The AGS cells were treated with varying concentrations of BMKNE. Analyses of the sub G1 peak, the caspase-3 and -9 activities, and the mitochondrial depolarization were conducted to determine whether AGS cell death occured by apoptosis. Also, to identify the role of transient receptor potential melastatin (TRPM) 7 channels in AGS cell growth and survival, we used human embryonic kidney (HEK) 293 cells overexpressed with TRPM7 channels. Results: Experimental results showed that the sub G1 peak, the caspase-3 and -9 activities, and the mitochondrial depolarization were increased. Therefore, BMKNE was found to induce the apoptosis of these cells, and this apoptosis was inhibited by SB203580 (a p38 mitogen-activated protein kinase (MAPK) inhibitor), and by a c-jun NH2-terminal kinase (JNK) II inhibitor. Furthermore, BMKNE inhibited TRPM7 currents and TRPM7 channel over-expressions in HEK 293 cells, exacerbating BMKNE-induced cell death. Conclusions: These findings indicate that BMKNE inhibits the growth and the survival of gastric cancer cells due to a blockade of the TRPM7 channel's activity and MAPK signaling. Therefore, BMKNE is a potential drug for treatment of gastric cancer, and both the TRPM7 channel and MAPK signaling may play an important role in survival in gastric cancer cells.

• Journal of Electrochemical Science and Technology
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• 제11권3호
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• pp.291-309
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• 2020

Chromium was electrodeposited from deep eutectic solvents-based Cr³⁺ electrolytes on HB-pencil graphite electrode. Factors influencing the electrochemical behavior and the processes of Cr nucleation and growth were explored using cyclic voltammetry and chronoamperometry techniques, respectively. Cr³⁺ reduction was found to occur through an irreversible diffusion-controlled step followed by another irreversible one of impure diffusional behaviour. The reduction behavior was found to be greatly affected by Cr³⁺ concentration, temperature, and type of hydrogen bond donor used in deep eutectic solvents (DESs) preparation. A more comprehensive model was suggested and successfully applied to extract a consistent data relevant to Cr nucleation kinetics from the experimental current density transients. The potential, the temperature, and the hydrogen bond donor type were estimated to be critical factors controlling Cr nucleation. The nucleation and growth processes of Cr from either choline chloride/ethylene glycol (EG-DES) or choline chloride/urea (U-DES) deep eutectic solvents were evaluated at 70℃ to be three-dimensional (3D) instantaneous and diffusion-controlled, respectively. However, the kinetics of Cr nucleation from EG-DES was found to be faster than that from U-DES. Cr nucleation was tending to be instantaneous at higher temperature, potential, and Cr³⁺ concentration. Cr nuclei electrodeposited from EG-DES were characterized at different conditions using scanning electron microscope (SEM). SEM images show that high number density of fine spherical nuclei of almost same sizes was nearly obtained at higher temperature and more negative potential. Energy dispersive spectroscopy (EDS) analysis confirms that Cr deposits were obtained.

• 전기화학회지
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• 제5권3호
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• pp.143-152
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• 2002

Self-assembled monolayer(SAM)로 변형된 금 전극 위로 폴리피롤의 전기화학적 석출 과정을 수용액 상태에서 in-situ EQCM (Electrochemical Quartz Crystal Microbalance)과 ex-situ AFM (Atomic Force Microscopy)을 이용하여 조사하였다. 금 전극에서 cyclic voltammetry로 살펴본 폴리머의 석출은 산화 제한 전위 (anodic limiting potential) 값에 매우 의존적이었으며 주사 횟수에는 의존하지 않았다. 제한 산화 전위가 0.8V (vs Ag | ArCl) 이상일 때 폴리머의 석출은 크게 증가하였다. 그리고 주사 횟수가 증가하면서 질량의 비이상적 변화가 관찰되었는데 이것은 폴리피롤 필름의 rearrangement가 원인이라고 생각된다. 1-dodecanethiol SAM 전극과 thiophene SAM전극에서는 폴리머가 3차원적으로 성장하며 필름의 rearrangement를 수반하였지만 BPUS $(Bis(\omega(N-pyrrolyl)-n-undecyl)disulfide)$ SAM 전극에서는 2차원적인 layer-by-layer 성장을 하고 필름의 rearrangement는 관찰되지 않았다. 폴리머가 급격하게 전극 면으로 석출되면 사슬 모양과 도너츠 모양의 폴리머를 만들며, 정류 상태에 이르면서 주름잡힌 폴리머 필름이 생성되는 것이 원자 힘 현미경 (Atomic Force Microscopy) 이미지로 관찰되었다.

• 한국항해항만학회지
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• 제31권5호
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• pp.377-383
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• 2007

선사들의 자가 터미널 확보, 전략적 제휴 및 M&A, 선박의 대형화는 항만으로 하여금 기항지 축소와 항만간 경쟁을 초래하게 하였다. 이러한 해운 항만의 환경변화는 선사들이 항만평가 기준인 항만선호도, 성장가능성 항만경쟁력 등에 대한 평가도 변하게 하고 있다. 선사들의 항만평가 기준의 변화는 항만이 추구해야 하는 정책방향을 결정하기 때문에 중요한 연구의 주제라고 할 수 있다. 따라서 본 연구에서는 ANVOA 분석과 Duncan 검증을 통해 선사들의 항만 기항선호도, 성장가능성, 항만경쟁력의 평가변수들을 대상으로 전세계 8개 주요항만(싱가포르항, 홍콩항, 상하이항, 부산항, 로테르담항, 함부르크항, 카오슝항, 도쿄항)을 유사 항만군으로 그룹핑하여 평가하고자 한다. 이는 평균차이 검증을 통해 선사들이 전세계 주요 항만을 평가하는 객관적인 평가결과로 사용될 수 있을 것이다.

• 한국작물학회지
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• 제42권2호
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• pp.146-152
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• 1997

Development of shoot and root, leaf water potential and photosynthetic rate affected by water stress in early growing stage of tobacco were surveyed to interpret stress response in terms of plant physiological and agricultural aspects. The growth of shoot and root was highly suppressed by water stress and the difference in dry weight by rewatering was smaller in root than in shoot. The total root length was highly decreased by water stress and the lengths of root for water stress and non-stress were 74m and 84m, respectively, after rewatering. The root growth treated by water stress was increased between 2nd and 3rd day after treatment indicating that temporary water stress at early growing stage might have increased of root zone activity for early growth stage. The leaf water potentials were decreased to -7.63MPa, -9.47MPa, -11.89MPa, -13MPa at the 2nd, 3rd, 4th and 5th day by water stress. The relative water contents were 75%, 62% and 57% at the 3rd, 4th and 5th day after treatment. Photosynthesis was reduced largely by water stress. The photosynthetic rate after treatment at 2nd day and 3rd day was dropped to 18.15$\mu$mol. $CO_2$/$m^2$ㆍsec$^{-1}$ and 9.35$\mu$mol. $CO_2$/$m^2$ㆍsec$^{-1}$. It was never recovered to the normal, even after rewatering. Stomatal conductance had been reduced since 2nd day after treatment and increased after rewatering.

• 한국토양비료학회지
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• 제47권4호
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• pp.290-298
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• 2014

A bacterial strain with the potential ability to solubilize heavy metals was isolated from heavy metal contaminated soils collected from abandoned mines of Boryeong area in South Korea. The bacterial strain with the highest degree of metal resistance was shown to have close proximity with Shewanella xiamenensis FJ589031, according to 16S rRNA sequence analysis, and selected for investigating the mobilization of metals in soil or plant by the strain. The strain was found to be capable of solubilizing metals both in the absence and in the presence of metals (Co, Pb and Cd). Metal mobilization potential of the strain was assessed in a batch experiment and the results showed that inoculation could increase the concentrations of water soluble Co, Pb and Cd by 48, 34 and 20% respectively, compared with those of non-inoculated soils. Bacterial-assisted growth promotion and metal uptake in sunflower (Helianthus annuus) was evaluated in a pot experiment. In comparison with non-inoculated seedlings, the inoculation led to increase the growth of H. annuus by 24, 18 and 16% respectively in Co, Pb and Cd contaminated soils. Moreover, enhanced accumulation of Co, Pb and Cd in the shoot and root systems was observed in inoculated plants, where metal translocation from root to the above-ground tissues was also found to be enhanced by the strain. Plant growth promotion and metal mobilizing potential of the strain suggest that the strain could effectively be employed in enhancing phytoextraction of Co, Pb and Cd from contaminated soils.

• 한국균학회지
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• 제26권3호통권86호
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• pp.387-395
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• 1998

여러가지 기주에서 유쾌한 Calonectria ilicicola 균주의 병원성에 대한 형태적, 유전적 표지를 검출하기 위하여 균사생장, 균핵, 자낭각 그리고 dsRNA를 조사하였다. 시험된 모든 균주에서 병원성, 균핵수, 자낭각수 와 균사생장 등의 다양한 변이가 관찰되었으나 dsRNA는 검출되지 않았다. 균의 병원성은 균핵과 자낭각 생산과 각각 정의 상관관계가 있었으나, 균사 생장은 자낭각의 생산과 부의 상관관계를 보여 주었다. 땅콩에서 유래한 균주보다 콩에서 유래한 균주가 기주인 콩에 더 강한 병원성을 보였으며 균핵과 자낭각의 생산도 더 많았다. 이와 같은 결과로 볼 때 C. ilicicla의 균핵과 자낭각 생산력은 inoculum potential의 구성요소로써 작용하며, 이중 균의 자낭각 생산력은 병원성과 기주 분화를 나타내는데 유용한 표지로써 이용될 수 있을 것이다.

• Current Research on Agriculture and Life Sciences
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• 제20권
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• pp.65-70
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• 2002

본 연구는 allelopathic potential을 지닌 벼의 교잡종을 포장에서 재배하여 그 식물체의 잎을 생물검정에 이용하여 allelopathy 효과 유무를 검정하고, 생물검정으로 선발된 교잡종을 대상으로 phenolic compounds를 조사하여 allelochemical로써의 가능성을 확인하고 나아가 phenolic compounds 생합성에 관여하는 효소의 활성 측정과 이 효소의 작용에 의해 유도되는 phenolic compounds를 구명한 결과는 다음과 같다. 1. 포장상태에서 피를 대상으로 생물검정을 실시한 결과 양친으로는 Kouketsumochi, Woo co chin yu에서 공통적으로 90% 이상의 높은 피뿌리 생장 억제율을 보였고, 교잡종에서는 Kouketsumochi/Woo co chin yu, Dongjinbyeo/Kouketsumochi, Dongjinbyeo/Woo co chin yu 조합에서 80% 이상의 높은 억제율을 나타냈다. 2. Kouketsumochi의 PAL 활성은 protein 1kg당 $63.46{\mu}kats$로 타 공시품종보다 높았다. 또한 Kouketsumochi와의 교잡종도 타교잡종보다 높은 것으로 나타났다. 3. Kouketsumochi의 cinnamate 함량은 생체중 1g당 $2.64{\mu}g$으로서 다른품종이나 교잡종보다 2~5배 높았고, Kouketsumochi와의 교잡종도 타교잡종보다 높았다.

• 한국양봉학회지
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• 제33권4호
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• pp.261-268
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• 2018

Queen honey bees (Apis cerana) can be reared artificially on demand by the use of grafting technique. The technique consists of grafting young worker larvae into queen cell cups and raising in a queenless strong colony. As the age of grafted larvae for queen rearing exhibited several aspects related to quality and reproductive potential of queen, this study were conducted to investigate the influence of age of grafted larva on morphological characteristics and lifespan of queen, and the growth of colony she headed. Our results demonstrated that queens reared from young worker larvae (i.e., less than 1-day old larvae) were significantly larger in size (i.e., body weight and thorax width) than that of queens reared from 2-day old worker larvae. Moreover, queens reared from younger worker larvae initiated egg-laying earlier, stored more spermatozoa in spermatheca and had a longer lifespan compared to queens raised from older worker larvae. We also found a significant positive effect of queen grafting age on the production of worker and drone brood, adult worker population in colonies headed by queens reared from younger larvae. These findings suggested that rearing queens from brood grafted at the earliest possible age could increase the reproductive potential of queen as well as fitness of colony she head.

• 대한치위생과학회지
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• 제5권2호
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• pp.85-96
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• 2022

Background: This study was aimed at determining the relationship of the Dominance, Influence, Steadiness, and Conscientiousness (DISC) personality type with the major satisfactions and job preferences of dental hygiene and technician students. Methods: We enrolled 264 dental hygiene and technician students from a junior college in Chungcheong-do. A questionnaire survey was conducted to determine the DISC behavior type, major satisfaction level, and job preference of the participants. Statistical analyses were performed with the independent-samples t-test, one-way analysis of variance, and multiple regression. Results: Dental technician students had higher major satisfaction (p< .001) and preferred jobs with potential for growth (p= .020) or matching their vocation and interests (p= .013) compared to dental hygiene students. Factors influencing major satisfaction were grade (β= .160, p= .008) and a social personality type (β= .146, p= .070). Factors influencing preference for jobs with high growth potential were the personality type (β= .236, p= .001), grades (β= .157, p= .002), and major satisfaction (β= .429, p< .001). The conscientiousness personality type was conducive to preferencefor jobs with high growth potential. The steadiness personality type was conducive to preference for jobs aligned with vocation and interest (β= .249, p= .004). The conscientiousness personality type (β= .137, p= .041) and high major satisfaction (β= .193, p= .003) were conducive to preference for jobs with a satisfactory working environment. Conclusion: The results of this study could serve as a basis for customized career counseling and education programs according to personality types.