• Korean Journal of Medicinal Crop Science
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• v.12 no.1
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• pp.43-46
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• 2004

In genus Angelica, three species have been used and cultivated for medicinal material in orient, A. gigas in Korea, acutiloba in Japan and sinensis in China. A. gigas (Chamdangui) is different from A. acutiloba or A. sinensis in the contents chemical components and their efficacious. In Korea, in particular, A. acutiloba has been cultivated in a small acreage from several decades ago, and recently A. sinensis has been trying to cultivate. A trial of adaptation and cultivation of the three species was performed in different altitudinal regions such as Suwon (50 m of sea level), Jinbu (500 m of sea level) and Taebak (700 m of sea level). A. gigas and acutiloba grew and produced roots successfully at all locations, but A. sinensis could grow only at high altitudinal region, Taebaek. A. sinensis survived 70.3% at Taebaek, but only 45.2% at Jinbu and 3.6% at Suwon. The dry root yield of A. sinensis was as low as 1,600 kg/ha at Taebaek and 1,270 kg/ha at Jinbu comparing farmers' average root yield of A. gigas, 3,000 kg/ha however, no root could be harvested at Suwon. The results, showed it was that A. sinensis could not grow at low altitudinal plane area except high altitude area in Korea. Even though it could grow, the root yield was too low to support the income.

• Korean Journal of Soil Science and Fertilizer
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• v.19 no.1
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• pp.56-62
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• 1986

Fertilization response on production and quality of flue-cured tobacco as to different level of nitrogen, phosphorus, and potassium were studied in a field experiment. The results were summarized as follows: 1. Growth and yield of flue-cured tobacco were significantly better in higher nitrogen fertilization levels, regardless of soil fertility, but the negative correlation was recognized between the quality of leaves and the amounts of nitrogen application. While, both fertilizers of phosphorous and potassium did not have should little effect on the tobacco yield and quality. 2. The optimum ratio of N, P, and K fertilizer applications were decided by the appearance of the proper yield and the best quality of tobacco leaves. The proportion of N:P:K was 2:1:4. 3. The single effect (Complete plot minus Non-fertilized plot) of N, P, and K on yield and quality of cured leaf was greatly affected by nitrogen, but the combined effect (Nutrient deficiency plot minus Non-fertilzier plot) of that were only slightly affected by P and K.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.14 no.2
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• pp.186-190
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• 2014

Citrullinemia type 2 (citrin deficiency) is an autosomal recessive inborn error metabolism, caused by the SLC25A13 gene mutation. Citrin deficiency is associated with two clinical phenotype; neonatal-onset type II citrullinemia (CTLN2), also known as neonatal intraphepatic cholestasis caused by citrin deficiency (NICCD) and adult-onset CTLN2. Clinical manifestations of NICCD include poor growth, intrahepatic cholestasis, liver dysfunction and increased plasma citrulline, methionine, threonine, arginine. The molecular diagnosis could be confirmed by SLC25A13 gene mutation analysis. A 3-month-old male infant with persistent jaundice was referred for evaluation. Newborn screening was normal at birth. Mild elevation of serum ammonia and AST/ALT were observed. Plasma amino acid analysis showed significantly elevated citrulline, methionine, threonine. DNA sequence analysis of the SLC25A13 gene revealed two compound heterozygous mutations, c.[852_855del]($p.Met285Profs^*2$) and [1180+1G>A]. We suggest that NICCD should be considered as one of the cause of in infants with cholestatic jaundice, although the newborn screening was normal.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.18 no.1
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• pp.23-29
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• 2018

Carbamoyl phosphate synthetase 1 deficiency (CPS1D) is a rare autosomal recessive urea cycle disorder characterized by hyperammonemia. CPS1D is caused by mutations in the CPS1 gene on chromosome 2q35. Based on the age of onset, there are two phenotypes: the neonatal type and the delayed-onset type. The severity of clinical manifestation depends on the degree of CPS1 residual enzymatic activity, and can result in hyperammonemia and neurological dysfunction. We report a case of CPS1D in a neonate who developed vomiting, decreased consciousness and hyperammonemia at 25th day after birth. She showed excellent response to treatment including hydration, ammonia-lowering drugs and a low-protein diet without hemodialysis. Her growth, development and neurological outcomes were fair at the last follow-up at 17 months of age.

• Journal of Mushroom
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• v.13 no.3
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• pp.228-232
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• 2015

'Nongjin-go' is a new breed strain of Lentinula edodes, saw-dust bag variety. It is a cross combination of dikaryon Lentinula edodes ASI 3305mut and monokaryon L5-16 of L. edodes ASI 3305(Sanjo701ho). We crossbred them by 2011 and verified productive capacity from 2012 to 2013 in Rural Development Administration. Optimum temperature of mycelial growth is $30^{\circ}C$ and it of fruit-body primodium formation is range from 15 to $23^{\circ}C$. Nongjigo is agricultured at mid-high temperature well. Fruiting body is platy-hemisphere, light brown and centralizing. And bast is formed around edge of pileus. Yield productions per period is regular than 'Sanjo701'. Plastic bag culture medium is 1.5 kilogram and culture periods are 90~100 days. As its browning of pileus in culture is a little slow, Light and ventilation is needed a lot in light-culturing. Humidity is controlled properly for its color in fruit-body growing. Tested culture medium is consisted of 80% Oak-Tree saw-dust and 20% rice-bran.

• Journal of Mushroom
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• v.10 no.4
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• pp.151-159
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• 2012

The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, 'Seolgang' was developed by crossing two monokaryons 'CM020913-27' and 'SSU423-31'. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of 'Seolgang' on CDA was better at $20^{\circ}C$ and $25^{\circ}C$ when it was compared with that of '505 Ho'. The mature cap shape of new strain 'Seolgang' is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain '505 Ho', the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of '505 Ho'. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of '505 Ho'. Genetic analysis of the new strain 'Seolgang' showed different profiles compared to '505 Ho', CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.

• Journal of Mushroom
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• v.12 no.1
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• pp.17-23
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• 2014

pH of oriental medicine sludge was 5.3, which was similar to 5.2 of the main ingredient, corncob. Its sugar content, however, was 4.8 mg/g, which was 2.5 times higher than concorb's 1.9 mg/g. According to the addition content analysis of oriental medicine sludge by using blood agar plate, the experimental group showed much more robust growth than the control group. 10% of oriental medicine sludge was added to corncob and pine tree sawdust for test-tube culture. Then they were cultivated at $25^{\circ}C$ for 6 days after inoculating Flammulina velutipes liquid spawn. The control group and experimental group showed 2.2~3.4 and 5.8~6.4 cm hypae growths respectively. At the field test for 10% herbal medicine refuse, mushroom yield dropped by 5% compared to the control group. However, it had distinctively lower number of deformity and the 2nd grade products. An economic analysis was conducted based on the cultivation facility that produces 160,000 mushrooms per day. The analysis demonstrated that the facility can save 50,000,000 won in the starting year and 130,000,000 won in the following years from the unit cost of production excluding labour and operation cost.

• Journal of Mushroom
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• v.12 no.1
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• pp.12-16
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• 2014

This study was carried out to develop the new mushroom cultivation medium for Pleurotus ostreatus using by EFB. Two strains of Pleurotus ostreatus were used for this study and the difference in productivity was observed in each strain. There was not confirmed a mycelial growth inhibition by addition of Empty Fruit Bunch which is palm tree waste. In the productivity test of the mixed medium, In Suhan 1ho, yield of T2 treatment was highest as 90.6 g per bottle, It was 14.9% higher than control. In Kimje variety which is Chunchu strain, Yield of T2 treatment was highest as 139.8 g per bottle. It was 25.2% higher than control. In Kimje variety, as amount of 6mm diameter PEFB pellet is increased in medium, yield of mushroom was decreased. When 8mm diameter EFB was used for 532 medium, yield of both 50% EFB(T3) and 100% EFB (T5) treatment was better than other treatments. Therefore, it is suggested that 8 mm diameter EFB pellet is good material for mushroom cultivation medium and T5 treatment was showed a potential possibility as an alternative medium.

• Journal of Mushroom
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• v.11 no.4
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• pp.208-213
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• 2013

The contents of raw materials which are components of mixed substrate for mushroom cultivation were analyzed to optimize the composition. The pure protein(amino acid) level of soybean meal was the highest, 44.02% followed by those of soybean curd residue(31.5%) and cotton seeds meal(30.6%). The non protein nitrogen(NPN) contents in crude protein of main nitrogen materials were 2.4% for soybean meal and 5.6% for dried soybean curd residue, while those of wheat bran and rice bran used as the carbon source were relatively higher, 17.6% compared to that of nitrogen supplying media. Crude protein content per price was 6.0 for rapeseed meal, indicating that it is high crude protein content per price. Nitrogen-free extract(NFE) considering as an ingredient for mycelial growth were high in alphacorn(72.9%) and wheat bran B(57.2%). Acid detergent fiber(ADF) was high in corncob, 51.88%, its use for cultivation of brown rot fungi including Lentinus lepideus should pay attention because the fungi lack complete lignin degradation activity.

• Molecular & Cellular Toxicology
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• v.4 no.4
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• pp.278-284
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• 2008

The X-linked inhibitor of apoptosis (XIAP) is a member of a novel family of inhibitors of apoptosis and has several BIR domains (BIR1, BIR2, and BIR3) and a carboxy-terminal RING zinc-finger. Since suppressionof apoptosis is fundamentally important for carcinogenesis and tumor growth, we investigated the expression and function of XIAP in DEN-induced carcinogenesis using rat model. Wistar rats were injected intraperitoneally with DEN at a dose of 50 mg/kg in twice a week for 12 weeks (Group II) and 16 weeks (Group III) followed by the recovery periods, respectively. The evaluation of DEN-induced carcinogenesis carried out the blood, RT-PCR, histopathological and western blot analysis. The level of blood chemistry including GOT/GPT, albumin, and total bilirubin were significantly exchanged comparing to control and Group I/Group II. The expression of albumin and collagen mRNA were significantly exchanged (P<0.05) in both groups. In addition, AFP mRNA expression decreased more after recovery periods than Group II. XIAP was expressed constitutively in normal rat liver as well as DEN-induced Groups I and Group II. In addition, XIAP expression increased more in Group I with 4 weeks recovery periods than Group I. However, XIAP expression shown to increase in Group lI, otherwise, it was decreased in Group II with 10 weeks repair periods. Taken together, these results suggest the alteration of XIAP expression could be involved in hepatocellular carcinogenesis.