• Korean Journal of Animal Reproduction
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• v.22 no.1
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• pp.1-9
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• 1998

This study was carried out to confirm whether the developmental capacity of bovine mature oocytes frozen ultra-rapidly using electron microscopic(EM) grids and EFS30 can be obtained, and whether the cryoprotectants and the freezing method used in this study effect detrimentally to the bovine oocytes by indirect immunocytochemistry. As freezing solution, we used EFS30 which consisted of 30% ethylene glycol, 0.5 M sucrose, 18% ficoll and 10% FBS added in D-PBS. The results obtained in this experiment were summarized as follows: When the effects of cryoprotectant and freezing procedure on the microtuble, micrfilament and chromatin morphology of oocytes were evaluated using indirect immunocytochemistry, the results of freezing as well as exposure group were not different with that of the control oocytes. When the fertilization abnormality after ultrarapid freezing of bovine mature oocytes was examined by Hoechst staining, the rates of total penetration(96.7, 9.0%), normal two pronuclei formation(74.6, 68.9%) and mean number of sperm / oocyte(1.50, 1.44) were not different between control and freezing group. In addition, when the developmental capacity of frozen-thawed of oocytes(85.5%) was survived, 74.5% of them were cleaved and 31.4% of cleaved embryos were developed to blastocyst. These data were similar to those of the control(76.0%, 34.6%) and exposure(74.5%, 33.0%) except survival rates. Also, when the total cell number of blastocysts produced from the each treatment at day after IVF was examined by hoechst staining, there were not different among groups. There results demonstrate that developmental capacity of frozen-thawed bovine mature oocytes can be successfully obtained by ultra-rapid freezing method using EM grid and EFS30 solution.

• The Transactions of the Korean Institute of Power Electronics
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• v.11 no.3
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• pp.193-200
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• 2006

This paper suggests a 6-pulse-shift converter structure with PWM current-source inverter based on buck-boost configuration to improve the efficiency and to reduce the switching frequency of inverter for photovoltaic generation system, the device can be operated as interface system between solar module system and power system grid without energy storage cell. The circuit has six current-source buck-boost converter which operate chopper part and kas one full bridge inverter which make a decision the polarity of AC output. Therefore, the proposed PWM power inverter has advantages such as the reduction of witching loss and realization of unity power factor operation. The theoretical backgrounds are discussed and the input-output characteristics for the implemented prototype inverter using TMS320F2812 are verified experimentally in this paper.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.7
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• pp.723-733
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• 2011

A numerical method of the CSF(Continuum Surface Force) model is presented for the calculation of the surface tension force and implemented in an in-house solution code(PowerCFD). The present method(code) employs an unstructured cell-centered method based on a conservative pressure-based finite-volume method with volume capturing method(CICSAM) in a volume of fluid(VOF) scheme for phase interface capturing. The application of the present method to a 2-D liquid drop problem is illustrated by an equilibrium and nonequilibrium oscillating drop calculation. It is found that the present method simulates efficiently and accurately surface tension-dominant multiphase flows.

• KIEAE Journal
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• v.16 no.6
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• pp.159-166
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• 2016

Purpose: The architectural structures in the engineering field include more than one material, and the heat transfer through these multiple materials becomes complicated. More or less, the analytic solutions obtained by the hand calculation can provide the limited information of heat transfer phenomena. However, the engineers have generally been forced to obtain reliable results than those of the hand calculation. The numerical calculation such as a finite volume methods with the unstructured grid system is only the suitable means of the analysis for the complex and arbitrary domains that consists of multiple materials. In this study, a new numerical code is developed to provide temperature distributions in the multiple material domains, and the results of this code are compared with the validation cases in ISO10211. Method: Finite volume methods with an unstructured grid is employed. In terms of numerical methods, the heat transfer conduction coefficient is not defined on the surface of the cell between different material cells. The heat transfer coefficient is properly defined to accurately mimic the heat transfer through the surface. The boundary conditions of heat flux considering radiation or heat convection are also developed. Result: The comparison between numerical results and ISO 10211 cases. We are confirmed that the numerical method provides the proper temperature distributions, and the heat transfer equation and its boundary conditions are developed properly.

• Fisheries and Aquatic Sciences
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• v.21 no.8
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• pp.24.1-24.9
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• 2018

The Korean bottom trawl survey has been deployed on a regular basis for about the last decade as part of groundfish stock assessments. The regularity indicates that they sample groundfish once per grid cell whose sides are half of one latitude and that of one longitude, respectively, and whose inside is furthermore divided into nine nested grids. Unless they have a special reason (e.g., running into a rocky bottom), their sample location is at the center grid of the nine nested grids. Given data collected by the survey, we intended to show how to appropriately estimate not only the survey index of a fish stock but also its uncertainty. For the regularity reason, we applied the systematic sampling theory for the above purposes and compared its results with a reference, which was based on the simple random sampling. When using the survey data about 11 fish stocks, collected by the spring and fall surveys in 2014, the survey indices of those stocks estimated under the systematic sampling were overall more precise than those under the simple random sampling. In estimates of the survey indices in number, the standard errors of those estimates under the systematic sampling were reduced from those under the simple random sampling by 0.23~27.44%, while in estimates of the survey indices in weight, they decreased by 0.04~31.97%. In bias of the estimates, the systematic sampling was the same as the simple random sampling. Our paper is first in formally showing how to apply the systematic sampling theory to the actual data collected by the Korean bottom trawl surveys.

• Journal of Veterinary Clinics
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• v.29 no.1
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• pp.27-32
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• 2012

The purpose of this study was to evaluate survival rates of vitrified mouse blastocysts in various vitrification solutions (cryoprotectants) and apparatuses. The mouse blastocysts were harvested from culture of mouse 2 cell embryo and were divided into three group (i) untreated (control); (ii) exposed to cryoprotectant agents; or (iii) cryopreserved by various vitrification apparatuses. Vitrification solutions are 40% ethylene glycol (EG) + 5.8 mg/mL ficoll + 0.5M sucrose (EFS solution), 3M glycerol + 3M EG (ES solution), 20% EG + 20% dimethyl sulfoxide (ED solution), 3M EG + 1.0 m sucrose (ES solution). Vitrification apparatuses consisted of 5 groups ; closed plastic straw (CPS), electron microscope (EM) grid, cryoloop, open pulled straw (OPS), and glass micropippete in plastic straw (GPS). The survival rates of control were 88.0%. The survival rates of exposed blastocysts in EFS, GE, ED, and ES solutions were 70.8%, 43.5% (P<0.01), 83.3% and 65.2%, respectively. The survival rates of vitrified blastocysts in CPS, EM grid, cryoloop, OPS and GPS were 56.5% (P< 0.01), 72.7%, 83.3%, 60.9% (P<0.05) and 54.2% (P<0.01), respectively. Among the vitrification solutions, the highest survival rate was seen in blastocysts vitrified in EG + DMSO (83.3%). The survival rate was not significantly different from that of the control (88%). Blastocysts cryopreserved with glycerol in all groups had an overall low survival rate of 43.5%. Survival rate of mouse blastocysts between vitrification apparatuses showed higher in cryoloop.

• Journal of Korea Society of Industrial Information Systems
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• v.16 no.2
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• pp.65-72
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• 2011

In this paper, a real-time monocular vision based navigation system is developed for the disabled people, where online background learning and vector field histogram are used for identifying obstacles and recognizing avoidable paths. The proposed system is performed by three steps: obstacle classification, occupancy grid map generation and VFH-based path recommendation. Firstly, the obstacles are discriminated from images by subtracting with background model which is learned in real time. Thereafter, based on the classification results, an occupancy map sized at $32{\times}24$ is produced, each cell of which represents its own risk by 10 gray levels. Finally, the polar histogram is drawn from the occupancy map, then the sectors corresponding to the valley are chosen as safe paths. To assess the effectiveness of the proposed system, it was tested with a variety of obstacles at indoors and outdoors, then it showed the a'ccuracy of 88%. Moreover, it showed the superior performance when comparing with sensor based navigation systems, which proved the feasibility of the proposed system in using assistive devices of disabled people.

• Korean Chemical Engineering Research
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• v.53 no.2
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• pp.164-173
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• 2015

This study aims to design energy supply systems from various energy sources for transportation sectors and comparatively analyze the life cycle cost of different scenario-based systems. For components of the proposed energy supply system, we consider a typical oil refinery, byproduct hydrogen system, renewable energy source (RES)-based electric generation system and existing electricity grid. We also include three types of vehicles in transportation sector such as internal combustion engine vehicle (ICEV), electric vehicle (EV), fuel cell vehicle (FCV). We then develop various energy supply scenarios which consist of such components and evaluate the economic performance of different systems from the viewpoint of life cycle cost. Finally we illustrate the applicability of the proposed framework by conducting the design problem of energy supply systems of Jeju, Korea. As the results of life cycle cost analysis, EV fueled by electricity from grid is the most economically feasible. In addition, we identify key parameters to contribute the total life cycle cost such as fuel cost, vehicle cost, infra cost and maintenance cost using sensitivity analysis.

• Electrical & Electronic Materials
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• v.7 no.3
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• pp.243-251
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• 1994

ITO(Indium Tin Oxide) film with thickness of 1500.angs. was prepared by an e-beam evaporator onto a glass and a p-type InP wafer (100) LEC grown Zn-doped p=2.3*10$\^$16/cm$\^$-3/), in which the components of ITO used for evaporation source were hot pressed pellets 1 mole% ln$\_$2/O$\_$3/+9 mole% SnO$\_$2/, and evaporated in O$\_$2/ ambient. The optimum conditions to preparation of ITO thin film were the substrate temperature of 350.deg. C, the injected oxygen pressure of 2*10$\^$-4/ torr, and the evaporation speed of 0.2-0.3.angs./sec, respectively. In these optimum conditions, the resistivity and the carrier concentration were 5.3*10$\^$-3/ .ohm.-cm, 6.5*10$\^$20/cm$\^$-3/, and the transmittance was over 80%. From the results of J-V measurements in ITO/p-InP structure solar cells, the higher pressure of injected oxygen, the more open circuit voltage. The efficiency of ITO/p-InP solar cell without the grid line contact, prepared by the optimum evaporation conditions, was 7.19%. By using the grid line contact, the efficiency, the open circuit voltage, the short circuit current density, the fill factor, the series resistance, and the shunt resistance were 8.5%, 0.47V, 29.48 mAcm$\^$-2/ , 61.35%, 3.ohm., and 26.6k.ohm., respectively.

• Journal of Korea Water Resources Association
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• v.32 no.2
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• pp.99-110
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• 1999

The method of moments, a Lagrangian scheme, considers the zeroth, first, and second moments of the grid cell spatial distributions of the concentration and then advects the concentration by maintaining conservation of the moments. The reasonable inital description of the first and second moments as well as the mean concentration, the zeroth moments, in grid element is important in the method of moments. In this study, the description methods of each initial moment are reviewed, and the method of moments is extended to overcome the restrictions of Courant number. Its performance is compared with those of available Eulerian and Lagrangian schemes. As the results, the method is successfully extended to overcome the stability restriction and is an accurate scheme for the advection simulation of concentration distribution, especially of which the gradient is steep. In addition, the method is very promising scheme in terms of computational efficiency when the mixing is confined in a relatively small region to the entire domain in two-dimensional problem.