• Journal of the Society of Cosmetic Scientists of Korea
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• v.36 no.3
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• pp.233-240
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• 2010

Derivatives of a novel natural compunds, melanostatin (PLG-$NH_2$) were prepared by solid phase synthesis[1,2] and assayed to evaluate their melanogensis inhibitory activity. Also, a small library (natural compound-XLG-$NH_2$, natural compound-X LG-OH) was prepared with same method for increasing synthetic yield and cost-reduction. PLG-$NH_2$ (Proline-Leucine-Glycine-$NH_2$) was well-known tripeptide as its $\alpha$-MSH release-inhibiting activity and tyrosinase inhibitory activity[3-5]. In order to choose best candidate for peptide derivatization, various natural compounds were screened by their tyrosinase inhibitory activity. As a result, caffeic acid and coumaric acid were selected. Most of these derivatives showed better activities than the parent natural compound, melanostatin.

• Journal of the Korean Applied Science and Technology
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• v.33 no.1
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• pp.51-57
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• 2016

In this study, we synthesized reactive surfactant which have functional radical group for effective chemical reaction. Reactive surfactant have been synthesized using synthesis of polyoxyethylene lauryl ether(POE 23) which is nonionic surfactant and methacrylic acid, acrylic acid. benzene was used as the solvent, p-TsOH was used as the catalyst. synthesized surfactant was confirmed by FT-IR, $^1H$-NMR spectra, and elemental analysis. Evaluation of physical properties was measured HLB, cloud point, surface tension, the critical micelle concentration, emulsifying power. HLB number was evaluated 11.62 to 12.09 range. The Critical Micelle Concentration(cmc) values evaluated was $1{\times}10^{-4}{\sim}5{\times}10^{-4}mol/L$ by surface tension method. The cloud point was $35^{\circ}C$, $39^{\circ}C$ each. The emulsifymvcqa ing properties of the synthesized surfactants was lower than polyoxyethylene lauryl ether. In addition, soybean oil was better than benzene. The experimental results confirmed the ester bond, the yield of 93.27%, 94.49% was found.

• Korean Chemical Engineering Research
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• v.43 no.2
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• pp.187-201
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• 2005

Bioprocessing technologies utilizing 'biorecognition' between a solid matrix and a protein is being widely experimented as a means to replacing the conventional, solution-based technology. Frequently the matrices are chromatographic resins with specific functional groups exposed outside. Since the reactions of and interactions with the proteins occur as they are attached to the solid matrix, this 'solid-phase' processing has distinct advantages over the solution-phase technology. Solid-phase refolding of inclusion body proteins uses ion exchange resins to adsorb denaturant-dissolved inclusion body. As the denaturant is slowly removed from the micromoiety around the protein, it is refolded into a native, three-dimensional structure. Once the refolding is complete, the folded protein can be eluted by a conventional elution technique such as the salt-gradient. This concept was successfully extended to 'EBA (expanded bed adsorption)-mediated refolding,' in which the denaturant-dissolved inclusion body in whole cell homogenate is adsorbed to a Streamline resin while cell debris and other impurity proteins are removed by the EBA action. The adsorbed protein follows the same refolding steps. This solid-phase refolding process shows the potential to improve the refolding yield, reduce the number of processing steps and the processing volume and time, and thus improve the overall process economics significantly. In this paper, the experimental results of the solid-phase refolding technology applied to several biopharmaceutical proteins of various types are presented.

• Korean Journal of Environment and Ecology
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• v.28 no.6
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• pp.634-641
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• 2014

In order to elucidate the ecological and phenological responses of bean under global warming situation, we investigated leafing and flowering phenomena and growth traits of Baktae and Seomoktae in control plot (ambient $CO_2$ concentration and temperature) and warmed plot (elevated $CO_2$ concentration and increased temperature than control) in green house. Average $CO_2$ concentration and temperature were set 540 ppm in treatment and higher $2.2^{\circ}C$ in treatment than control. The appearance of leaf unfolding and the initiation of blooming and fruit maturing in Seomoktae were late in treatment than control. In case of Baktae, the number of total pods and seeds per plant in treatment was fewer than that in control. For Seomoktae, the number of total pods per plant and weight of total seeds per plant were decreased in treatment than in control. Thus, the number of pods and seeds of Baktae declined and the production of Seomoktae was decreased under global warming condition. This result indicates that site selection and crop yield for cultivating of the bean may be changed into new area due to global warming condition.

• Journal of Bio-Environment Control
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• v.14 no.2
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• pp.119-127
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• 2005

This study was carried out to investigate the effect of magnesium concentrations in fertilizer solution on growth and cut flower quality of chrysanthemum 'Biarritz'. The nutrient concentrations in plant tissue and soil solution of root media were also determined. Magnesium deficiency appeared on older leaves with interveinal yellow-green chlorosis. Marginal chlorosis and necrosis also developed on some of older leaves. Elevation of Mg concentration in fertilizer solution increased cut flower weight at harvesting stage resulting in the 8.84g in 1.5mM treatment. Dry weight of whole above ground plant tissue increased as Mg concentrations in fertilizer solution were elevated within the range from 0 to 15mM, but that of 20mM decreased compared to 15mM treatment. The dry weight of 0.5, 1.0 and 1.5mM treatments were 8.42, 8.75 and 8.848 and tissue Mg contents of those based on the youngest fully expanded leaves at harvesting stage were 0.34, 0.53 and $0.71\%$, respectively. Based on dry weight and tissue Mg contents, Mg fertilization to maintain tissue contents higher than $0.64\%$ is necessary to ensure flower quality and yield. By considering the concentration in 15mM treatment, Mg concentration in soil solution of root media should be higher than $3.68mg{\cdot}L^{-1}$ at harvesting stage.

• Food Science and Preservation
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• v.24 no.6
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• pp.834-841
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• 2017

This study was carried out to investigate the anti-oxidative and anti-inflammatory effects of hot water extracts of Ligularia fischeri cultivated in Youngyanggun. We obtained hot water extract (HWE) and cold water extract (CWE) from L. fischeri. The anti-oxidative activities of L. fischeri extracts were measured by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. The anti-inflammatory effects of L. fischeri were evaluated in human mast cell line-1 (HMC-1) cells stimulated with phorbol-12-myristate-13-acetate plus A23187 (PMACI). The solid yields of HWE was 150% higher than CWE solid yield. Total polyphenol contents of HWE were $198.07{\pm}0.24mg/g$. The value of anti-oxidative activities of HWE were shown $IC_{50}$ $28.2{\pm}0.04ug/mL$. We showed that HWE significantly reduced the PMACI-induced the production of IL-6 (0.01-1 mg/mL), IL-8 (0.1-1 mg/mL), and $TNF-{\alpha}$ (0.01-1 mg/mL). These results indicate that the HWE of L. fischeri can be used as a functional material due to its antioxidant and anti-inflammatory activities.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.147-156
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• 1981

Most of the currently available rice threshers in Korea are of head-feeding type originally developed for threshing paddy at grain moisture content of 15 to 17% on wet basis. And prevailing rice harvesting system is swathing or cutting and bundling upright rice crop at a maturity stage of grain moisture content of ca. 28 to 20%, followed by transient natural drying in the field and threshing at grain moisture content of about 17%. The system often allows rather high field loss of rice both quantitatively and qualitatively. These necessitate use of a preharvest desiccant, and diquat has been registered recently as a rice desiccant. Aim of the present study is to determine the best time and rate of diquat application and to evaluate its utility as a rice desiccant under its optimal usage conditions in Korea. Diquat was not so effective for japonica rice varieties while it was very effective for the new varieties bred from indica x japonica crosses. The best time of application was the period from 2 days before to 3 days after physiological maturity of grain (moisture content of 28% w.b.) and the optimal rate was about 330g of diquat ion/ha. Applying diquat at the right time and rate dropped grain moisture to 15 to 16% (w.b.) within a week after treatment in contrast to that of 20 to 24% in untreated rice. Desiccation of rice crop with diquat resulted in 10 to 15% increase in grain resistance to shattering and significant reduction in percent green rice. However, there was no reduction in milled rice yield and percent whole kernel and was no change in the percent chalky rice and percent tinted rice. Diquat use appears highly desirable, if there is no residue problem which has not been studied in the present study.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.124-127
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• 2008

A new orange-colored, cut flower gerbera (Gerbera hybrida Hort.) cultivar 'Sweety' was developed from a cross between 'Grandeur' and 'Songsongee', followed by selections of seedlings and lines at the Flower Breeding Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 1999 to 2004. Three time evaluations were conducted from 2003 for the detailed characteristics of the new cultivar. 'Sweety' has semi-double type mini- or medium-sized flowers in good harmony with orange(RHS, 38-A) ray florets and a green center. It has good, stable flower shape and strong peduncle, and its vase life was 10.4 days. The average flower yield was about 51.0 stems per plant/year in greenhouse trails during 2003 and 2004. This cultivar was registered to the Korea Seed and Variety Service for commercialization in 2006. Year-round production of this cultivar is possible in the greenhouse condition in Korean climate.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.699-702
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• 2010

Anthracnose is a major limiting factor of the watermelon production in Korea. A mid-late maturing watermelon line, 'Hanbit' with resistance to anthracnose (Colletotrichum orbiculare) race 1 and 3, was developed by the cross between anthracnose resistant cultivar 'AU-Producer' and high quality inbred line '920533' at the National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA). 'Hanbit' produces 5.4 to 9.5 kg of red flesh fruits with clear stripes on skin. Average soluble solid contents are ranged from 10.4 to 11.2$^{\circ}$Bx. The yield and quality of 'Hanbit' was comparable to or better than those harvested from the popular commercial cultivars. 'Hanbit' can be cultivated in open fields and under the protected green house condition. In addition to the commercial production, it can be used as a resistant material in $F_1$ hybrid breeding program.

• Korean Journal of Plant Resources
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• v.35 no.4
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• pp.445-454
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• 2022

The aim of this study was to provide basic data for breeding new varieties of Angelica gigas Nakai by identifying the growth characteristics and useful components of its below- and above-ground parts. The basic varieties expressing dark purple (N79-A), light blue-pink (62-C), and white (NN115-C) flowers were identified and collected in the cultivation area, and their seeds were cultured for use as experimental materials. Qualitative growth characteristics of the above-ground parts were then examined. Purple coloration was evident throughout the entire plant body in the N79-A group, whereas the 62-C and NN115-C groups displayed yellow-green coloration. In terms of quantitative growth characteristics, the 62-C group displayed significantly lower above- and below-ground growth than the other two groups. Levels of nodakenin and decursinol-angelate were high in the NN115-C group (0.88 ± 0.13%) and N79-A group (2.56 ± 0.12%). However, the overall results were not statistically significant. The results could provide a foundation for breeding new varieties of Angelica gigas Nakai, which are used predominantly for medicinal purposes and have low bolting and high yield potential.