• KSCE Journal of Civil and Environmental Engineering Research
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• v.43 no.5
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• pp.675-687
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• 2023

The border area between the South and North Korea is considered a stage for promoting Green Détent through the transformation of the Demilitarized Zone into the Green Peace Zone. This paper proposes 'Prosuming-index' composed of items to evaluate the multi-layered nature of industrial and infrastructure projects in the ecological and environmental fields planned in the border area. Based on the prosuming-index, we derived the following four types of potential for Green Detent practice in the border area-first, the Incheon and Ganghwa regions as 'a logistics/human network and agricultural/fishery production area', second, the northern Gyeonggi region as a 'cooperative network area based on the smart technology industries', third, the western Gangwon and the parts of nothern Gyeonggi region, as 'a multiple prosumer's area based on carbon neutral technologies', lastly, the eastern Gangwon region, as 'a transition area from idle or aged infrastructure to green infrastructure through the renewable energy industries'.

• Journal of the Korean Institute of Telematics and Electronics A
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• v.29A no.7
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• pp.64-72
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• 1992

The capacitance for three-dimensional (3D) VLSI interconnection line is calculated. Capacitance is obtained by solving integral equation that is the product of Green's function and surface charge density. Surface charge density is assumed that constant in each subarea, and subarea is devided by rectangular size in interconnetion surfaces. Up to date, so this integral method using Green's function is calculated by Fourier integral transformation, that it cannot help making an error. In this paper, it is proposed to use direct integration instead of Fourier integral method. And we proved accuracy of this paper in comparision with conventional results.

• Journal of Plant Biotechnology
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• v.2 no.3
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• pp.135-142
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• 2000

The activity of promoter sequences was evaluated in garlic cells using the $\beta$-glucuronidase (GUS) gene as a reporter. Histochemical GUS assay indicated transient GUS activity in leaf, callus and root cells 48 hours after particle bombardment transformation. Quantitative fluorometric assays in extracts of transformed leaves demonstrated that the CsVMV promoter induced the highest level of gene expression, which was, on average, ten fold the level induced by CaMV35S and by the Arabidopsis Act2 promoters and two fold the level expression observed with a construct containing a double CaMV35S plus the untranslated leader sequence from AMV. No activity or very low levels were observed when cells were transformed with plasmids rontaining the typical monocot promoters, Actl, from rice or the Ubi-1, from maize. The green fluorescent protein (GFP) was also tested as a marker gene for garlic transformation. Intense fluorescence was observed in leaf, callus and root cells transformed with a construct containing the gfp gene under control of the CaMV35 Promoter. No fluorescence was detected when the gfp was under control of the Ubi-1 promoter.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.2
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• pp.119-127
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• 2013

The interaction between Agrobacterium and soybean has been studied at the transcriptome level but not at the metabolic level. However, it is necessary to investigate the difference in metabolites between susceptible and non-susceptible cultivars for high efficiency transformation. We investigated the difference in metabolites from sonicated soybean cotyledons of Korean cultivars and Bert cultivar. To identify difference in metabolites, sonicated extracts were analysed by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS). The soybean cultivars were classified by susceptibility using green fluorescent protein expression. We found a difference in metabolites between the high susceptible and low susceptible cultivars. The FT-ICR/MS experimental m/z data of different metabolites were compared with theoretical m/z in KNApSAcK database. The candidate list was made using KNApSAcK and focused on phenolic compounds. These candidate metabolites are speculated to influence factors in the interaction. This list of candidates may be useful to investigate the interaction between Agrobacterium and plants to increase transformation efficiency.

• The Plant Pathology Journal
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• v.21 no.1
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• pp.13-20
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• 2005

Chlorella viruses are large icosahedral, plaque-forming, dsDNA viruses that infect certain unicellular, chlorellalike green algae. The genomic DNA of over 300 kb contains many useful genes and promoters. Over 40 chlorella viruses have been isolated from fresh water in Korea since 1998. The viruses were amplified initially in chlorella strain NC64A, and pure isolates were obtained by repeated plaque isolation. SDS-PAGE analysis revealed similar but distinct protein patterns, both among the group of purified viruses and in comparison with the prototype chlorella virus PBCV-1. Digestions of the 330- to 350-kb genomic DNAs with 10 restriction enzymes revealed different restriction fragment patterns among the isolates. The tRNA-coding regions of 8 chlorella viruses were cloned and sequenced. These viruses contain 14-16 tRNA genes within a 1.2- to 2-kb region, except for the SS-1 isolate, which has a 1039-bp spacer in a cluster of 11 tRNA genes. Promoter regions of several early genes were isolated and their activities were analyzed in transformed chlorella. Some promoters showed stronger activity than commonly used CaMV 35S promoter and chlorella transformation vectors for heterologous protein are beings constructed using these promoters.

• Journal of Sensor Science and Technology
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• v.21 no.1
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• pp.28-33
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• 2012

The visual sensing of human hands plays an important part in many man-machine interaction/interface systems. Most existing visionbased hand detection techniques depend on the color cues of human skin. The RGB color image from a vision sensor is often transformed to another color space as a preprocessing of hand detection because the color space transformation is assumed to increase the detection accuracy. However, the actual effect of color space transformation has not been well investigated in literature. This paper discusses a comparative evaluation of the pixel classification performance of hand skin detection in four widely used color spaces; RGB, YIQ, HSV, and normalized rgb. The experimental results indicate that using the normalized red-green color values is the most reliable under different backgrounds, lighting conditions, individuals, and hand postures. The nonlinear classification of pixel colors by the use of a multilayer neural network is also proposed to improve the detection accuracy.

• Journal of the Korean Society of Visualization
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• v.8 no.4
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• pp.26-30
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• 2010

A PTV algorithm was constructed using a linear transformation, in which the merits of the conventional PIV and PTV were adopted. In PIV calculations, the obtained velocity vectors are affected by the filtering effects by its calculation principle. PTV techniques are widely used for their excellences of measuring small scaled flows, such as nano and bio flows. However, PTVs produce vector errors due to interpolation process. To overcome these problems, a hybrid PTV algorithm was constructed by combining PTVs' and PIVs' benefits using a linear transformation. The Taylor-Green vortex flows were generated for the tests of vorticity calculations. The conventional gray-level cross-correlation PIV technique and 2-Frame PTV technique were tested for the same flows for comparisons with those obtained by the constructed hybrid algorithm. The excellence of the constructed hybrid algorithm was validated through an actual experiment on the cylinder wake.

• Journal of Electrical Engineering and information Science
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• v.3 no.3
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• pp.330-335
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• 1998

In this paper, a new light-value approaching method to enhance a color image by excluding the effect of incident illumination is proposed. The method uses the fundamental vector transformation in which an estimated color of illumination is rotated to the white color of natural daylight. Then the transformed red, green, and blue values of each pixel are nonlinearly mapped into the 8-bit values to enhance intensity and saturation in the dark portion of the image. The proposed algorithm can produce the enhanced color image fast and efficiently without any space conversion or noticeable distortion.

• Journal of Plant Biotechnology
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• v.33 no.2
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• pp.75-84
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• 2006

Heteroplasmic rice plastid transformant was generated using suspension cells as bombardment materials. PCR analyses confirmed incorporation of aadA and cp4-epsps genes into the rice plastid genome by homologous recombination events via the flanking sequences of the trnI and trnA. Transplastomic calli were actively proliferated when cultured on AAM2 medium supplemented with various concentrations (500-3000 mg/L) of streptomycin in dark condition, and transplastomic suspension cells showed resistance to nonselective herbicide, glyphosate. Through 'agarose pie selection' method, heteroplastomic calli, containing considerably high level of transplastome and expressing the CP4 EPSPS protein, were obtained. They were further regenerated to green shoots with healthy roots.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.145.3-146
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• 2003

Transgenic Eleutherococcus senticosus plants were prepared by introducing the genes for squalene synthase (SQS), hygromycin phosphotransferase (HPT) and green fluorescent Protein (GFP) through Agrobacterium-mediated transformation. The enzyme, SQS, represents a putative branch point in the isoprenoid pathway capable of diverting carbon flow specifically to the biosynthesis of phytosterol and oleanolic acid. The full SQS gene was isolated from P. ginseng roots. Early globular embryo clusters developed from embryogenic callus were used as the explant source. (omitted)