• Parasites, Hosts and Diseases
• /
• 제57권4호
• /
• pp.379-387
• /
• 2019

Clonorchis sinensis is a carcinogenic human liver fluke that promotes hepatic inflammatory environments via direct contact or through their excretory-secretory products (ESPs), subsequently leading to cholangitis, periductal fibrosis, liver cirrhosis, and even cholangiocarcinoma (CCA). This study was conducted to examine the host inflammatory responses to C. sinensis ESPs and their putative protein components selected from C. sinensis expressed sequenced tag (EST) pool databases, including $TGF-{\beta}$ receptor interacting protein 1(CsTRIP1), legumain (CsLeg), and growth factor binding protein 2 (CsGrb2). Treatment of CCA cells (HuCCT1) with the ESPs or bacterial recombinant C. sinensis proteins differentially promoted the secretion of proinflammatory cytokines ($IL-1{\beta}$, IL-6, and $TNF-{\alpha}$) as well as anti-inflammatory cytokines (IL-10, $TGF-{\beta}1$, and $TGF-{\beta}2$) in a time-dependent manner. In particular, recombinant C. sinensis protein treatment resulted in increase (at maximum) of ~7-fold in $TGF-{\beta}1$, ~30-fold in $TGF-{\beta}2$, and ~3-fold in $TNF-{\alpha}$ compared with the increase produced by ESPs, indicating that CsTrip1, CsLeg, and CsGrb2 function as strong inducers for secretion of these cytokines in host cells. These results suggest that C. sinensis ESPs contribute to the immunopathological response in host cells, leading to clonorchiasis-associated hepatobiliary abnormalities of greater severity.

• 천문학회보
• /
• 제35권2호
• /
• pp.36.2-36.2
• /
• 2010

CQUEAN (Camera fo QUasars in EArly uNiverse) is a newly developed camera by CEOU for the 2.1m telescope at the McDonald Observatory, Texas, USA. We report the early science results from the commissioning run of CQUEAN which include the observations of the gamma-ray burst (GRB) afterglows and quasars at z ~ 5.5. Although the data were originally taken to test the instrument performance, the results are already very encouraging. We uncovered GRB afterglows at z = 0.8 - 1.4, with our data being used for the international collaboration research to understand the nature of GRBs. The unique filter sets we employed are providing the data which are effective for selecting quasars at z ~ 5.5. The special aspects of CQUEAN - high sensitivity at 0.8-1.1 ${\mu}m$ and fast readouts - will allow us to produce many interesting through surveys of high redshift quasars and fast follow-up of transient objects such as GRBs and exoplanets in future.

• 천문학회보
• /
• 제37권2호
• /
• pp.208.2-208.2
• /
• 2012

The Slewing Mirror Telescope (SMT) is a key telescope of Ultra-Fast Flash Observatory (UFFO) space project to explore the first sub-minute or sub-seconds early photons from the Gamma Ray Bursts (GRBs) afterglows. The first realization of UFFO is the 20kg UFFO-Pathfinder (UFFO-P) to be launched on board the Russian Lomonosov satellite in 2013 by the Soyuz-2 rocket. Once the UFFO Burst Alert & Trigger Telescope (UBAT) detects the GRBs, Slewing mirror (SM) will rotate to bring the GRB into the SMT's field of view instead of slewing the entire spacecraft. SMT can image the UV/Optical counterpart with about 4-arcsec accuracy. However it will provide a important understanding of the GRB mechanism by measuring the sub-minute optical photons from GRBs. SMT can respond to the trigger over $35^{\circ}{\times}35^{\circ}$ wide field of view within 1 sec by using Slewing Mirror Stage (SMS). SMT has 10-cm Ritchey-Chretien telescope and $256{\times}256$ pixilated Intensified Charge-Coupled Device (ICCD) on focal plane. In this paper, we discuss the overall design of UFFO-P SMT instrument and payloads development status.

• 천문학회보
• /
• 제37권2호
• /
• pp.208.1-208.1
• /
• 2012

The Ultra Fast Flash Observatory (UFFO) pathfinder is a payload system on-board the Russian satellite Lomonosov, scheduled to be launched in 2013. The main purpose of the UFFO pathfinder is to observe the early photons from Gamma-Ray Bursts. It consists of two instruments. The first instrument is the UFFO Burst Alert X-ray Trigger telescope (UBAT) for the fast-trigger and detection of GRB location, and the second is the Slewing Mirror Telescope (SMT) for the observation of the UV/optical afterglow from the GRB located by the UBAT. It will provide the first-ever systematic study of UV/optical emission far earlier than 1 sec after trigger. We will present the design, fabrication and the preliminary performance of the UBAT.

• 천문학회보
• /
• 제41권1호
• /
• pp.61.1-61.1
• /
• 2016

As a new way to explore the universe, astronomers are now performing time-domain astronomy by surveying the universe looking for new transient phenomena and taking movies of the universe with telescopes. Large-area, time-series survey of astronomical objects are uncovering many interesting, fast-changing objects that have now been poorly understood before, such as GRBs, tidal disruption phenomena, and new types of supernova. In order to characterize these new, exciting events, it is very critical to perform follow-up observations, and 1-2m telescopes can effectively contribute to such efforts. Since 2007, our group has been performing follow-up observations of gamma-ray bursts (GRBs) and interesting transients using BOAO and other KASI facilities. Here, we present results from several key transient studies that were done by using BOAO: (1) tidal disruption event Swift J1644+57; (2) SN 2011fe that occurred in M101; and (3) several GRB events. These study demonstrates the usefulness of BOAO as a powerful transient follow-up facility. Finally, we will discuss how BOAO research activities can possibly be bolstered in this newly emerging field of astronomy.

• 천문학회보
• /
• 제43권1호
• /
• pp.39.1-39.1
• /
• 2018

Although it is agreed that the gamma-ray bursts (GRBs) invoke highly relativistic jets with bulk Lorentz factors of a few hundreds, the exact physical mechanism producing such powerful gamma-rays still remains debated. Three outstanding and important questions in the field concern (1) the composition of GRB jets (i.e., matter-dominated vs Poynting-flux-dominated), (2) the involved radiative process responsible for the observed gamma-rays (i.e., synchrotron mechanism vs photospheric radiation), and (3) the distance of the emitting region from the central engine where the prompt gamma-rays are released (i.e., ~10^12 cm vs 10^14 cm vs 10^16 cm). I will present recent important breakthroughs that we have made, which answer these three questions.

• Molecules and Cells
• /
• 제40권11호
• /
• pp.855-863
• /
• 2017

Adipose tissue plays a central role in regulating dynamic cross-talk between tissues and organs. A detailed description of molecules that are differentially expressed upon changes in adipose tissue mass is expected to increase our understanding of the molecular mechanisms that underlie obesity and related metabolic co-morbidities. Our previous studies suggest a possible link between endophilins (SH3Grb2 proteins) and changes in body weight. To explore this further, we sought to assess the distribution of endophilin A2 (EA2) in human adipose tissue and experimental animals. Human paired adipose tissue samples (subcutaneous and visceral) were collected from subjects undergoing elective abdominal surgery and abdominal liposuction. We observed elevated EA2 gene expression in the subcutaneous compared to that in the visceral human adipose tissue. EA2 gene expression negatively correlated with adiponectin and chemerin in visceral adipose tissue, and positively correlated with $TNF-{\alpha}$ in subcutaneous adipose tissue. EA2 gene expression was significantly downregulated during differentiation of preadipocytes in vitro. In conclusion, this study provides a description of EA2 distribution and emphasizes a need to study the roles of this protein during the progression of obesity.

• BMB Reports
• /
• 제47권10호
• /
• pp.581-586
• /
• 2014

Epidermal growth factor (EGF) is known to play key roles in skin regeneration and wound-healing. Here, we demonstrate that Pep2-YAC, a tripeptide covering residues 29-31 in the B loop of EGF, promotes the proliferation of HaCaT keratinocytes with activity comparable to EGF. The treatment of HaCaT cells with Pep2-YAC induced phosphorylation, internalization, and degradation of EGFR and organization of signaling complexes, which consist of Grb2, Gab1, SHP2, and PI3K. In addition, it stimulated the phosphorylation of ERK1/2 at Thr 202/Tyr 204 and of Akt1 at Ser 473 and the nuclear translocation of EGFR, STAT3, c-Jun, and c-Fos. These results suggest that Pep2-YAC may be useful as a therapeutic agent for skin regeneration and wound-healing as an EGFR agonist.

• Fisheries and Aquatic Sciences
• /
• 제21권2호
• /
• pp.4.1-4.5
• /
• 2018

Insulin-like growth factors (IGFs), along with IGF-binding protein and IGF receptor, are well-known regulators in the growth and survival of vertebrates. In this study, we investigated the involvement of IGFs and protein variation during embryonic development of the olive flounder (Paralichthys olivaceus). Morphological stages were divided into six main developments as blastula, gastrula, cephalization, cranial regionalization, tail lift, and hatch. During embryonic development, protein variation was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrospray ionization quadrupole time-of-flight mass spectrometry/mass spectrometry. In addition, the mechanism of signaling of IGF-I receptor was examined using immuno-blot analysis. We found marked changes in protein expression at four stages of embryonic development and identified proteins as belonging to the vitellogenin 2 family. As development progresses, expression of IGF-II, phosphotyrosine, and phospho-Akt increased, while expression of growth factor receptor-bound protein 2 (GRB2) and one of guanine-nucleotide-binding proteins (Ras) decreased. These results provide basic information on the IGF system in the embryonic development of the olive flounder.

• 한방안이비인후피부과학회지
• /
• 제21권3호
• /
• pp.52-68
• /
• 2008

Objectives and Methods : Salviae miltiorrhizae Radix (SMR) promotes blood circulation to remove blood stasis, cools the blood to relieve carbuncle, clears away heat from the heart and tranquilizes the mind. This study was designed to investigate the effects of SMR on immuno-potentiative action in terms of changes in the genetic profile of dendritic cells (DC) using by microarray analysis. Results and Conclusion: In this experiment, treatments with more than 250 ${\mu}g/ml$ upto 1000 ${\mu}g/ml$ of SMR elevated the proliferation rates of DC. Microscopic observations confirmed the tendency on proliferation rates. Expression levels of genes related with cellular methabolic process, cell communication, and macromolecule metabolic process were elevated by treatment with SMR in comparison of functional distribution in a Biological Process. In molecular functions, expression levels of genes related with receptor activation, nucleotide binding and nucleic acid binding were elevated. In cellular components, expression levels of genes related to cellular membrane-bound organelles were elevated. In addition, expression levels of genes related to Wnt signalling pathways and the glycerophospholipid metabolism were elevated through analysis using pathway analysis between up-and down-regulated genes in cells treated with SMR. Finally, genes related to JAK2, GRB2, CDC42, SMAD4, B2M, FOS and ESRI located the center of Protein interaction network of genes through treatment with SMR.