Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Protein variation and involvement of insulin-like growth factor during embryonic development in the olive flounder Paralichthys olivaceus

  • Kim, Kang-Woong (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Nam, Taek Jeong (Department of Food Science and Nutrition, Pukyong National University) ;
  • Choi, Youn Hee (Department of Marine Bio-Materials and Aquaculture, Pukyong National University)
  • Received : 2017.11.22
  • Accepted : 2018.01.03
  • Published : 2018.02.28
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Abstract

Insulin-like growth factors (IGFs), along with IGF-binding protein and IGF receptor, are well-known regulators in the growth and survival of vertebrates. In this study, we investigated the involvement of IGFs and protein variation during embryonic development of the olive flounder (Paralichthys olivaceus). Morphological stages were divided into six main developments as blastula, gastrula, cephalization, cranial regionalization, tail lift, and hatch. During embryonic development, protein variation was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrospray ionization quadrupole time-of-flight mass spectrometry/mass spectrometry. In addition, the mechanism of signaling of IGF-I receptor was examined using immuno-blot analysis. We found marked changes in protein expression at four stages of embryonic development and identified proteins as belonging to the vitellogenin 2 family. As development progresses, expression of IGF-II, phosphotyrosine, and phospho-Akt increased, while expression of growth factor receptor-bound protein 2 (GRB2) and one of guanine-nucleotide-binding proteins (Ras) decreased. These results provide basic information on the IGF system in the embryonic development of the olive flounder.

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References

  1. Caelers A, Berishvili G, Meli ML, Eppler E, Reinecke M. Establishment of a real-time RT-PCR for the determination of absolute amounts of IGF-I and IGF-II gene expression in liver and extrahepatic sites of the tilapia. Gen Comp Endocrinol. 2004;137:196-204. https://doi.org/10.1016/j.ygcen.2004.03.006
  2. Chandra V, Kumar GS, Sharma GT. Temporal expression pattern of insulin-like growth factors (IGF-1 and IGF-2) ligands and their receptors (IGF-1R and IGF-2R) in buffalo (Bubalus bubalis) embryos produced in vitro. Live Sci. 2011;135:225-30. https://doi.org/10.1016/j.livsci.2010.07.016
  3. Choi YH, Chang YJ. Gametogenic cycle of the transplanted-cultured pearl oyster, Pinctada fucata martensii (Bivalvia: Pteriidae) in Korea. Aquaculture. 2003;220:781-90. https://doi.org/10.1016/S0044-8486(02)00304-6
  4. Choi YH, Kim KW, Han HS, Nam TJ, Lee BJ. Dietary Hizikia fusiformis glycoproteininduced IGF-I and IGFBP-3 associated to somatic growth, polyunsaturated fatty acid metabolism, and immunity in juvenile olive flounder Paralichthys olivaceus. Comp Biochem Physiol A. 2014;167:1-6.
  5. Choi YH, Yamaguchi K, Oda T, Nam TJ. Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): protective activity of the N-terminal fragment of PYP1 against acetaminophen-induced cell death in Chang liver cells. Int J Mol Med. 2015;35:271-6. https://doi.org/10.3892/ijmm.2014.1992
  6. Duan C. The insulin-like growth factor system and its biological actions in fish. Amer Zool. 1997;37:491-503. https://doi.org/10.1093/icb/37.6.491
  7. Duan C, Xu Q. Roles of insulin-like growth factor (IGF) binding proteins in regulating IGF actions. Gen Comp Endocrinol. 2005;142:44-52. https://doi.org/10.1016/j.ygcen.2004.12.022
  8. Emmersen BK, Petersen IM. Natural occurrence, and experimental induction by estradiol-$17{\beta}$, of a lipophosphoprotein (vitellogenin) in flounder (Platichtys flesus, L.). Comp Biochem Physiol. 1976;54B:443-6.
  9. Fuentes EN, Bjornsson BT, Valdes JA, Einarsdottir IE, Lorca B, Alvarez M, Molina A. IGF-I/PI3K/Akt and IGF-I/MAPK/ERK pathways in vivo in skeletal muscle are regulated by nutrition and contribute to somatic growth in the fine flounder. Am J Physiol Regul Integr Comp Physiol. 2011;300:R1532-42. https://doi.org/10.1152/ajpregu.00535.2010
  10. Hardy K, Spanos S. Growth factor expression and function in the human and mouse preimplantaion embryo. J Endocrinol. 2002;172:221-36. https://doi.org/10.1677/joe.0.1720221
  11. Li M, Bureau DP, King WA, Leatherland JF. The actions of in ovo cortisol on egg fertility, embryo development and the expression of growth-related genes in rainbow trout embryos, and the growth performance of juveniles. Mol Reprod Dev. 2010;77:922-31. https://doi.org/10.1002/mrd.21239
  12. Li Y, Wu S, Ouyang J, Mao L, Li W, Lin H. Expression of insulin-like growth factor-1 of orange-spotted grouper (Epinephelus coioides) in yeast Pichia pastoris. Protein Expr Purif. 2012;84:80-5. https://doi.org/10.1016/j.pep.2012.04.019
  13. Manning BD, Cantley LCAKT. PKB signaling: navigating downstream. Cell. 2007;129:1261-74. https://doi.org/10.1016/j.cell.2007.06.009
  14. Martinez GM, Bolker JA. Embryonic and larval staging of summer flounder (Paralichthys dentatus). J Morphol. 2003;255:162-76. https://doi.org/10.1002/jmor.10053
  15. Matsubara T, Ohkubo N, Andoh T, Sullivan CV, Hara A. Two forms of vitellogenin, yielding two distinct lipovitellins, play different roles during oocyte maturation and early development of barfin flounder, Verasper moseri, a marine teleost that spawns pelagic eggs. Dev Biol. 1999;213:18-32. https://doi.org/10.1006/dbio.1999.9365
  16. Ohlsson C, Sjogren K, Jansson JO, Isaksson OGP. The relative importance of endocrine versus autocrine/paracrine insulin-like growth factor-I in the regulation of body growth. Pediatr Nephrol. 2000;14:541-3. https://doi.org/10.1007/s004670000348
  17. Riley JK, Carayannopoulos MO, Wyman AH, Chi M, Ratajczak CK, Moley KH. The PI3K/Akt pathway is present and functional in the preimplantation mouse embryo. Dev Biol. 2005;284:377-86. https://doi.org/10.1016/j.ydbio.2005.05.033
  18. Santos AN, Ramin N, Tonack S, Fischer B. Cell lineage-specific signaling of insulin and insulin-like growth factor I in rabbit blastocysts. Endocrinol. 2008;149:515-24. https://doi.org/10.1210/en.2007-0821
  19. Schlueter PJ, Peng G, Westerfield M, Duan C. Insulin-like growth factor signaling regulates zebrafish embryonic growth and development by promoting cell survival and cell cycle progression. Cell Death Differ. 2007;14:1095-105. https://doi.org/10.1038/sj.cdd.4402109
  20. Silversand C, Haux C. Isolation of turbot (Scophthalmus maximus) vitellogenin by high-performance anion-exchange chromatography. J Chromatogr. 1989;478A:387-97.
  21. Vera Cruz EM, Brown CL, Luckenbach JA, Picha ME, Bolivar RB, Borski RJ. Insulin-like growth factor-I cDNA cloning, gene expression and potential use as a growth rate indicator in Nile tilapia, Oreochromis niloticus. Aquaculture. 2006;251:585-95. https://doi.org/10.1016/j.aquaculture.2005.06.039
  22. Xu Y, Zang K, Liu X, Shi B, Li C, Shi X. Insulin-like growth factors I and II in starry flounder (Platichthys stellatus): molecular cloning and differential expression during embryonic development. Fish Physiol Biochem. 2015;41:139-52. https://doi.org/10.1007/s10695-014-0012-y
  23. Yuan Y, Hong Y. Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro. Sci Rep. 2017;7:78. https://doi.org/10.1038/s41598-017-00094-y.
  24. Zou S, Kamei H, Modi Z, Duan C. Zebrafish IGF genes: gene duplication, conservation and divergence, and novel roles in midline and notochord development. PLoS One. 2009;4:e7026. https://doi.org/10.1371/journal.pone.0007026

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