• Applied Biological Chemistry
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• 제41권8호
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• pp.578-582
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• 1998

Grape seed oil was characterized to assess the usefulness in the food industry. Among the various oils, the initial antioxidant activity was the highest for grape seed oil. Heating the oil at $180^{\sim}C$ for 20 min retained 86% of the initial activity. Grape seed and sesame oils showed a low peroxide value, about 2, implying a less oxidative reaction. The oxidation of grape seed oil was increased to a less extent by heat-treatment than other oils. Light exposure for 1 month resulted in a slight decrease in the antioxidant activity of grape seed oil, maintaining 96% of the initial activity. Other oils were all light-susceptible and the activities decreased significantly. The peroxide values of all the oils increased by light exposure, but the extent of oxidation was still the least for grape seed oil. The addition of grape seed oil to perilla oil was very effective, in that the peroxide value was 5-times decreased by 1 : 5 composition of grape seed oil versus perilla oil. These results indicate that grape seed oil can be used as a good cooking oil or an additive for other oils.

• Nutrition Research and Practice
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• 제4권2호
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• pp.114-120
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• 2010

The effects of grape seeds extract and grape peels extract prepared from grape pomace on the activity of antioxidant enzymes, degree of lipid peroxidation in serum and liver tissue were investigated in rabbits fed on high cholesterol diet. New Zealand white rabbits were divided as follows ; 1) NOR (normal group); 2) CHOL (cholesterol group); 3) GSH (cholesterol + grape seed extract group); 4) GPE (cholesterol + grape peel extract); 5) GSP (cholesterol + grape seed powder); 6) GPP (cholesterol + grape peel powder); 7) GE (cholesterol + grape seed and peel extract); 8) GP (cholesterol + grape seed and peel powder). Eight groups of rabbits were studied for 8 weeks. At the end of the experimental period, rabbits were sacrificed and the liver tissue were removed. Then, GSH, GPx, GST, CAT and MDA in the liver were measured. In liver tissues, total glutathione contents (GSH), glutathione peroxidase (GPx) and catalase (CAT) activity, which was significantly higher by grape seed extract supplementation. The level of malondialdehyde (MDA) was lower in the serum of rabbits fed grape seed extract or grape peel powder plus cholesterol than in the serum of rabbits fed cholesterol alone. It is therefore likely that grape seed extract prepared from grape pomace functioned as antioxidants in vivo, negating the effects of the oxidative stress induced by 1% cholesterol diet. The grape seed extract was found effective in converting the oxidized glutathione into reduced glutathione, and in removing $H_2O_2$ that is created by oxidative stress. The grape peel powder was found to have small influence on reduced glutathione content, CAT and GPX activity, but it increased GST activity in liver tissues, resulting in promoting the combination of lipid peroxide and glutathione (GSH), and further, lowering the formation of lipid peroxide in the serum. Therefore, grape pomace (grape seed extract and grape peel powder) supplementation is considered to activate the antioxidant enzyme system and prevent damage with hypercholesterolemia.

• Journal of Nutrition and Health
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• 제36권7호
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• pp.667-674
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• 2003

The study was performed to investigate the effects of isoflavone and/or grape seed oil supplementation on serum and liver lipid profiles and bone strength in ovariectomized female rats. Female Spraque-Dawley rats were assigned into 4 groups of ovariectomized (O), isoflavone (0.085 mg/100 g b.w/day) in ovariectomized rats, grape seed oil in ovariectomized rats and isoflavone and/or grape seed oil in ovariectomized rats. After 8 weeks, biochemical profiles of serum, liver and bone were analyzed. Total food intakes, body weight gains and FER (food efficiency ratio) were not statistically significantly different among groups. Total cholesterol, triglyceride and LDL-cholesterol levels in serum were decreased by fed of isoflavone and/or grape seed oils. However, crude lipid and total cholesterol contents in liver were not affect of isoflavone and/or grape seed oil. The hepatic glutathione contents were increased by isoflavone and/or grape seed oil fed. The hepatic glutathione-S-transferase activity in isoflavone and/or grape seed oil supplemented groups were higher than that O group. Bone (scapular and femur bone) dry weight, femur of max weight and bending strength were no significant difference among groups. Our finding suggest that isoflavone/grape seed oils might have potential role for serum lipid profiles improvement and bone strength in vivo.

• 한국식품영양과학회지
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• 제44권12호
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• pp.1813-1818
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• 2015

본 연구에서는 높은 불포화지방산 함유율로 말미암아 항산화 성분을 함유하고 있음에도 불구하고 산패에 대한 위험성을 내포하고 있는 포도씨유에 다량의 항산화 성분을 함유한 것으로 알려진 포도씨추출물을 첨가하여 포도씨추출물 함유 포도씨유의 90일 동안의 저장 중 산화 안정성을 측정하였고, 갈색화, 비타민 E, 지방산 조성 및 폴리페놀 함량 변화를 조사해 보았다. 포도씨추출물을 첨가한 포도씨유의 경우 무첨가 포도씨유보다 저장기간 동안 과산화물가, 산가 및 공액이중결합가가 모두 낮아 포도씨추출물이 포도씨유의 산화 안정성에 기여하는 것으로 나타났다. 저장 중 흡광도 증가량 역시 포도씨추출물 첨가 포도씨유가 무첨가 포도씨유에서보다 적게 나타났고, 비타민 E 이성체의 감소량 또한 무첨가 포도씨유에서보다 포도씨추출물 첨가 포도씨유에서 적게 나타났으며, 지방산 조성 변화 역시 무첨가 포도씨유에서보다 포도씨추출물 첨가 포도씨유에서 적게 나타났다. 한편 저장 중 폴리페놀 함량 감소량은 포도씨추출물 함유 포도씨유에서 가장 크게 나타났다. 따라서 포도씨추출물은 저장 중 포도씨유의 산화 안정성에 기여하는 것으로 확인되었으며, 다만 그 능력은 합성항산화제인 butylated hydroxytoluene보다는 낮은 것으로 조사되었다.

• 한국지역사회생활과학회지
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• 제18권3호
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• pp.363-368
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• 2007

This study is to find out the antioxidative effect and serum lipid composition of wild grape seed powder in vivo. 20 white Sprague Dawley rats of six weeks old were divided into 2 groups and AIN-93 basic diet, high fat and cholesterol were provided. And they were examined to know how wild grape seed powder worked for antioxidative effect and serum lipid composition. For the comparing group, wild grape seed powder consisting 5% of the diet weight was provided and the quantity of protein, fat, carbohydrate, and cellulose was controlled following the analysis of the ingredients. The rats were fed for four weeks with experimental diet. Serum lipid and the antioxidant enzyme activity in blood and liver microsome were measured after 4 weeks of experiment. The results are as follows; There was no difference between the experimental groups in the initial body weight, final body weight, weight gain and FER. Food intake was higher in the group wild grape seed powder was provided than in the control group(p<0.05). Serum total cholesterol in the control group was significantly higher than that in the group wild grape seed powder was provided.(p<0.05). There was no difference serum HDL cholesterol and LDL cholesterol between the groups. Serum triglyceride showed no significant difference between the groups. In blood, glutanthione peroxidase activity was higher in the group supplemented with wild grape seed powder than in the control group. The glutathione reductase activity of blood showed no difference between the groups. In liver, the glutanthione peroxidase activity was higher in the group supplemented with wild grape seed powder than in the control group(p<0.05). Glutathione reductase activity in liver showed no difference in accordance with the supplementation of wild grape seed powder.

• KSBB Journal
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• 제20권5호
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• pp.383-386
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• 2005

압착이나 유기용매 추출방식으로 생산된 포도씨유에는 resveratrol이 함유되어있지 않다 초임계 이산화탄소로 추출하는 경우 포도씨에 존재하는 resveratrol의 약 $1.6\%$가 추출 함유됨을 확인하였다. 초임계 추출 효율은 포도씨 건조 정도에 따라서 달라졌는데, 시료에 수분이 전혀 있지 않은 경우는 resvertrol을 추출되지 않았다. 수분함량이 증가할수록 추출 효율이 증가하는데, 수분이 resvertrol을 추출에 중요한 역할을 하였다. 초임계유체 추출 시간에 따른 추출된 포도씨 유의 resvertol 함량은 초반보다 후반으로 갈수록 증가하는 경향을 나타내었다. 초반에 오일 이 많이 추출되고 이후 수분이 빠져나오는 추출성향을 볼 때, resvertrol추출에는 수분이 초임계유체의 성질에 큰 영향을 미쳐 추출 효율을 변화시키는 것으로 생각된다.

• Nutrition Research and Practice
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• 제6권1호
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• pp.3-8
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• 2012

Polyphenol-rich grape seeds have a beneficial effect on human health. The present study was performed to investigate the effects of grape seeds on antioxidant activities in rats. Male Sprague-Dawley rats were randomly divided into a control diet group (C), a high-fat diet group (HF), a 5% grape seed-supplemented control diet group (G), and a 5% grape seed-supplemented high-fat diet group (HG). Dietary supplementation with grape seeds reduced serum concentrations of lipid peroxides compared with those in the C and HF groups. The hepatic level of lipid peroxides decreased significantly in the grape seed groups compared with that in the C and HF groups. Superoxide dismutase activity in the G group increased significantly compared with that in the C group. Catalase activity tended to be higher by feeding grape seeds. The grape seed diet increased glutathione peroxidase activity in the C group. Glutathione-S-transferase activity increased significantly in the G group compared with that in the C group. Hepatic content of total glutathione increased significantly in the HG group but decreased significantly in the HF group. The ratio of reduced glutathione and oxidized glutathione increased by feeding the grape seed diet. Total vitamin A concentration was significantly higher in HG group than in other groups. Liver tocopherol content of the G and HG groups was significantly higher than that of the control groups. These results suggest that dietary supplementation with grape seeds is beneficial for suppressing lipid peroxidation in high fat-fed rats.

• 한국식품저장유통학회지
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• 제12권6호
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• pp.600-607
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• 2005

포도씨을 이용한 고부가가치의 포도씨유 제조기술 개발 및 시제품을 생산하기위해 먼저 일반 재배 및 유기농 재배 포도로부터 각각 수거된 포도씨의 수율, 및 기능성성분(지방산, 피토스테롤, 토코페롤, 총카테킨 및 4가지 카테킨 조성)의 차이를 측정하여 비교하였다. 다음, 용매추출법과 화학적 정제방법에의한 포도씨로부터 고품질의 포도씨유의 제조기술을 개발하고 시제품을 생산하한 결과는 다음과 같다. 일반 재배 및 유기농 재배 포도로부터 얻어진 포도씨유 및 추출물의 수율 그리고 기능성성분(지방산, 피토스테롤, 토코페롤, 총카테킨 및 4가지 카테킨류)의 함량을 측정한 후 비교한 결과 대체적으로 일반 재배 및 유기농 재배 포도씨의 기능성성분의 함량에는 큰 차이가 없었다. 건조포도씨(1.0톤)를 볶음처리 후 분쇄하여 노르말-헥산으로 탈지하고 얻은 포도씨 원유를 탈검($0.15\%$ 인산) 및 탈산($20\%$ NaOH 용액) 처리 후 수증기증류 장치($240^{\circ}C$, 2시간, 5 mmHg)를 이용하여 탈색, 탈향 및 탈취공정을 차례로 거치면서 고품질의 포도씨유의 제조기술을 확립하였으며, 아울러 그로부터 최종 포도씨유 제품(50kg, 수율: $5.0\%$/건조 포도씨)을 생산하였다.

• Journal of Applied Biological Chemistry
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• 제42권4호
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• pp.175-179
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• 1999

Grape seed oil was extracted using different preparatory treatments as follows: (1) grinding, (2) grinding and roasting, (3) grinding and wet- roasting, (4) grinding, roasting, and wet-roasting, and (5) grinding, wet-roasting, and wet-roasting. The highest antioxidant activity was obtained from the sample with the method (2). Initial states of oxidation were similar except method (1) that showed more oxidized state, being P.O.V.8. Acid values were observed in the range from 1.42 to 1.89. The lowest acid value was found as 1.42 in method (1) and those of others were somewhat higher, indicating that heating process of roasting produced some free fatty acids. From the results of sensory evaluation, the best odor and taste were obtained from the methods (2) and (3). Repetitive procedure of wet-roasting, like method 5, caused some loss of flavor components and decrease in the sensory evaluation score. Addition of grape seed oil (method 2) to soybean and perilla oil at the level of 20% retained considerable antioxidant activities as much as 4.3 and 5 times, respectively, than 100% soybean or perilla oil stored for 12 weeks. When soybean or perilla oil was mixed with 20% grape seed oils, P.O.V. decreased to half of that of unmixed oils.

• Journal of Animal Science and Technology
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• 제50권5호
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• pp.721-732
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• 2008

본 연구는 프레스햄의 품질특성에 포도씨유 첨가가 미치는 영향을 조사하고자 실시하였다. 시험구는 프레스햄에 첨가되는 포도씨유의 양에 따라 5개의 시험구를 배치하였다. 대조구는 포도씨유를 첨가하지 않고 총 구성분 중 10% 량만큼 등지방을 첨가하였다. 처리구 1은 첨가되는 돈지방 함량 중 10%를 포도씨유로 대체, 처리구 2는 20%, 처리구 3은 30%, 처리구 4는 40%를 포도씨유로 대체하여 제조하였다. 제조된 프레스햄은 진공포장하여 냉장온도(4℃)에서 28일 동안 저장하면서 포도씨유가 첨가된 프레스햄의 전단가, 관능적 특성분석, 지방산패도 및 지방산 조성변화를 조사하였다. 전단가는 처리구간의 비교에서 저장 1일, 21일과 28일에는 T4 처리구가 대조구에 비하여 유의적으로 낮은 전단가를 보였다(P<0.05). 저장기간의 경과에 따른 변화에서는 전 처리구가 저장기간이 경과함에 따라 유의적인 차이는 있지만 뚜렷한 경향이 없었다. 관능검사 결과 제품색, 향기, 조직감 및 전체적인 기호성은 대조구와 포도씨유 처리구간에 유의적인 차이가 없었으며(P>0.05), 저장기간에 따른 제품의 제품색, 향기, 조직감 및 전체적인 기호성 변화는 모든 처리구가 저장기간이 경과하여도 유의적인 차이가 없었다(P>0.05). 지방산화는 처리구간의 비교에서 저장 28일에 T4 처리구가 대조구 비하여 유의적으로 낮은 지방산화를 보였다(P<0.05). 저장기간에 따른 지방산화 값의 변화는 대조구와 포도씨유 처리구 모두 저장기간이 경과함에 따라 유의적으로 높게 나타났다(p<0.05). 지방산 조성 변화는 대조구에 비하여 포도씨유 처리구는 C10:0~C20:4 지방산 함량이 유의적으로 감소하였다(P<0.05). 포화지방산과 단가불포화지방산 함량은 대조구가 포도씨유 처리구에 비하여 유의적으로 높은 함량을 보였으며(P<0.05), 포도씨유 처리구 간에는 대체 수준이 증가할수록 포화지방산 함량이 유의적으로 감소하였다(P<0.05). 다중불포화 지방산 함량은 대조구에 비하여 포도씨유 처리구가 유의적으로 높은 함량을 보였으며, 대체 수준이 증가할수록 다중불포화 지방산 함량이 유의적으로 증가하였다(P<0.05). 이상의 결과 프레스햄 제조시 포도씨유의 첨가는 관능적 평가 및 지질산화에 영향을 미치지 않으며, 제품의 지방산 조성을 변화시켜 건강지향적 고급 육제품 생산이 가능하다고 사료된다.