• Title/Summary/Keyword: Grafting

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Standardization of a Graft Inoculation Method for the Screening of Mungbean Germplasm against Mungbean yellow mosaic virus (MYMV)

  • Akhtar, Khalid Pervaiz;Ahsanul Haq, M.
    • The Plant Pathology Journal
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    • v.19 no.5
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    • pp.257-259
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    • 2003
  • This report described a simple, inexpensive, faster, and effective graft inoculation method for the artificial transmission of Mungbean yellow mosaic virus (MYMV). Success of grafting and disease transmission was 100% in this method. Screening of mungbean germplasm using this method will prevent the chance of escape infection, probably as a consequence of non-preference mechanism and loss of vector infectivity. The grafting method described here is applicable to both screenhouse and field trials.

The Right Gastroepiploic Artery Graft for Coronary Artery Bypass Grafting: A 30-Year Experience

  • Suma, Hisayoshi
    • Journal of Chest Surgery
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    • v.49 no.4
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    • pp.225-231
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    • 2016
  • Throughout its 30-year history, the right gastroepiploic artery (GEA) has been useful for in situ grafts in coronary artery bypass grafting (CABG). The early graft patency rate is high, and the late patency rate has improved by using the skeletonized GEA graft and proper target selection, which involves having a target coronary artery with a tight >90% stenosis. Total arterial revascularization with the internal thoracic artery and GEA grafts is an option for achieving better outcomes from CABG procedures.

Graft Polymerization of vinyl monomers onto silk (Vinyl단량체의 silk graft중합)

  • 남중희;마석일;이양후
    • Journal of Sericultural and Entomological Science
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    • v.19 no.1
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    • pp.33-36
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    • 1977
  • Graft polymerization of vinyl monomers onto silk were carried out in aqueous medium using ceric salt as an initiator, and discussed about the mechanical properties of silk. The results obtained are summarized as follows: 1. Percent grafting is greatly enhanced by the increasing of the temperature. 2. Effect of percent grafting is in the order of methyl methacrylate, and vinyl acetate. 3. Strength is decreased in the high grafted silk, and elongation is increased in the low grafted silk.

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Graft Copolymerization of Methyl Methacrylate on to Chloroprene Rubber (Chloroprene 고무에 대한 Methyl Methacrylate의 Graft공중합)

  • Park, Chan-Young;Kim, Byung-Kyu;Park, Tchun-Wook
    • Elastomers and Composites
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    • v.26 no.2
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    • pp.109-114
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    • 1991
  • The graft copolymerization of chloroprene rubber with methyl methacrylate in toluene solution has been carried out varying the concentration of monomer, initiator, CR, reaction time, and reaction temperature, etc.. It is observed that the grafting reaction follows conventional kinetic behavior under the present experimental conditions. Identification of grafting on to chloroprene rubber was obtained through characterization of the graft copolymer by infrared spectrometer.

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"Off-Pump" Coronary rtery bypass Grafting in Multi-vessel Coronary Disease -Two Cases- (다중 혈관질환에서 심폐바이패스를 이용하지 않은 관상동맥 우회술)

  • 유원희;김기봉
    • Journal of Chest Surgery
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    • v.32 no.12
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    • pp.1123-1126
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    • 1999
  • Coronary artery bypass grafting (CABG) technique has been much developed but CABG under cardiopulmonary bypass has the unavoidable deficits such as generalized inflammatory reaction from cardiopulmonary bypass and myocardial ischemia from aortic-cross clamp. There has been remarkable advancement of CABG without cadiopulmonary bypass. We performed CABG successfully without cardiopulmonary bypass. We performed CABG successfully without cardiopulmonary bypass in two patients with multivessel coronary disease who were failed to intervene with percutaneous transluminal coronary angioplasty. We herein report the two cases.

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Comparison of Resistance Level to Cotton leaf curl virus(CLCuV) Among Newly Developed Cotton Mutants and Commercial Cultivars

  • Akhtar, Khalid P.;Khan, Azeem I.;Hussain, M.;Khan, M.S.I.
    • The Plant Pathology Journal
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    • v.18 no.4
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    • pp.179-186
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    • 2002
  • Four newly developed cotton mutants (M-111, M-7662, M-358 and M-218) were compared for their resistance against Cotton leaf curl virus(CLCuV) together with commercial resistant (CIM-443, CIM-482, CIM-473, FH-900 and FH-901) and susceptible (5-12) varieties by artificial inoculation through grafting and under natural field conditions. Infectivity and success of grafting were 100% in all cases. None of the grafted plants were found immune or asymptomatic. All the grafted mutants and most of their single plant progeny rows (SPPRs) showed highly resistant responses as the symptoms displayed by these mutants were milder than the commercial cultivars. Grafted mutants also had delayed disease reactions as they took more time (25-30 days) to produce disease symptoms, as compared with resistant commercial varieties that produced disease 18-22 days after inoculation. Growth of the grafted SPPRs of tested mutants was normal, which is an indication that there will be no production losses. Observations under natural infestation of whitefly showed that two SPPRs of M-ll/CE and M-7662-1/2 and one resistant variety CIM-443 exhibited slight incidence of disease, while one SPPR of M-l1/59 and S-12 were moderately susceptible and highly susceptible with 21% and 97.l% disease incidence, respectively. This study also showed that plants displaying more disease symptoms through grafting were easily infected under natural conditions. These results suggest that preference should be given to those plants that exhibited highly resistant responses after artificial inoculation.

Synthesis and Characterization of Polypropylene-grafted Graphene Oxide via "Grafting-to" Method (폴리프로필렌으로 그래프트된 그래핀 옥사이드 제조 및 특성 분석)

  • Lee, Jong-Hee;Oh, Chang-Ho;Lim, Jung-Hyurk;Kim, Kyung-Min
    • Polymer(Korea)
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    • v.39 no.1
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    • pp.180-184
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    • 2015
  • PP-grafted GO was prepared by the reaction of graphene oxide (GO) containing 2-bromoisobuyryl groups and polypropylene (PP) having hydroxyl groups (PP-OH) via a "grafting-to" method. GO-Br was synthesized by the reaction of GO and 2-bromoisobutyryl bromide under a basic condition. PP-MAH was reacted with ethanolamine to produce PP-OH. The melting temperature of PP-grafted GO was shifted to the higher temperature than that of PP-OH. Also, the thermal stability of PP-grafted GO was increased as compared to PP-OH and GO. These results demonstrated that the grafted coating polymer PP was effective for enhancing the thermal stability of GO. The higher surface roughness of PP-grafted GO was resulted from the chemical attachment of PP on the surface of GO. The characterization of PP-grafted GO was conducted from Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and scanning electron microscope (SEM).

Silane-crosslinked Proton Exchange Membranes Prepared by a Stepwise Radiation Grafting (방사선 그래프트를 순차적으로 진행하여 제조된 실란 가교구조의 수소이온교환막)

  • Lee, Ji-Hong;Choi, Hongsuk;Song, Ju-Myung;Sohn, Joon-Yong;Shin, Junhwa
    • Polymer(Korea)
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    • v.36 no.6
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    • pp.816-821
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    • 2012
  • In this study, silane-crosslinked proton exchange membranes were prepared by step-wise radiation grafting of styrene and 3-(trimethoxysilyl)propyl methacrylate (TMSPM) onto an poly(ethylene-co-tetrafluoroethylene) (ETFE) film and followed by sol-gel processing and sulfonation. The sequentially grafted films with styrene to provide the proton conductivity and TMSPM to form the crosslinked structure were prepared by different grafting order. The structural changes and thermal properties of the prepared films were investigated by FTIR and TGA, respectively. After the introduction of sulfonic acid functional groups, the distributions of sulfonic acid group and silicon atoms at the inside of the sulfonated membranes were analyzed by SEM-EDX.

Functional Outcomes of Multiple Sural Nerve Grafts for Facial Nerve Defects after Tumor-Ablative Surgery

  • Lee, Myung Chul;Kim, Dae Hee;Jeon, Yeo Reum;Rah, Dong Kyun;Lew, Dae Hyun;Choi, Eun Chang;Lee, Won Jai
    • Archives of Plastic Surgery
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    • v.42 no.4
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    • pp.461-468
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    • 2015
  • Background Functional restoration of the facial expression is necessary after facial nerve resection to treat head and neck tumors. This study was conducted to evaluate the functional outcomes of patients who underwent facial nerve cable grafting immediately after tumor resection. Methods Patients who underwent cable grafting from April 2007 to August 2011 were reviewed, in which a harvested branch of the sural nerve was grafted onto each facial nerve division. Twelve patients underwent facial nerve cable grafting after radical parotidectomy, total parotidectomy, or schwannoma resection, and the functional facial expression of each patient was evaluated using the Facial Nerve Grading Scale 2.0. The results were analyzed according to patient age, follow-up duration, and the use of postoperative radiation therapy. Results Among the 12 patients who were evaluated, the mean follow-up duration was 21.8 months, the mean age at the time of surgery was 42.8 years, and the mean facial expression score was 14.6 points, indicating moderate dysfunction. Facial expression scores were not influenced by age at the time of surgery, follow-up duration, or the use of postoperative radiation therapy. Conclusions The results of this study indicate that facial nerve cable grafting using the sural nerve can restore facial expression. Although patients were provided with appropriate treatment, the survival rate for salivary gland cancer was poor. We conclude that immediate facial nerve reconstruction is a worthwhile procedure that improves quality of life by allowing the recovery of facial expression, even in patients who are older or may require radiation therapy.

Use of a Temporary Shunt as a Salvage Technique for Distal Extremity Amputations Requiring Repair by Vessel Grafting during Critical Ischemia

  • Ince, Bilsev;Dadaci, Mehmet;Altuntas, Zeynep
    • Archives of Plastic Surgery
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    • v.43 no.6
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    • pp.544-550
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    • 2016
  • Background Although the use of temporary shunts in proximal extremity amputations has been reported, no study has described the use of temporary shunts in distal extremity amputations that require vein grafting. Moreover, the total volume of blood loss when temporary shunts are used has not been reported. The aim of this study was to investigate the applicability of a temporary shunt for distal extremity amputations requiring repair by vessel grafting with an ischemia time of >6 hours. This study also aimed to determine the total volume of blood loss when temporary shunts were used. Methods Patients who underwent distal major extremity replantation and/or revascularization with a vessel graft and who experienced ischemia for 6-8 hours between 2013 and 2014 were included in the study. A 6-Fr suction catheter was cut to 5 cm in length after the infusion of heparin, and secured with a 5-0 silk suture between the distal and the proximal ends of the artery. While bleeding continued, the bones were shortened and fixed. After the complete restoration of circulation, the arterial shunt created using the catheter was also repaired with a vein graft. Results Six patients were included in this study. The mean duration of ischemia was 7.25 hours. The mean duration of suction catheter use during limb revascularization was 7 minutes. The mean transfusion volume was 7.5 units. No losses of the extremity were observed. Conclusions This procedure should be considered in distal extremity amputations requiring repair by vessel grafting during critical ischemia.