• Journal of Magnetics
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• 제21권1호
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• pp.125-132
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• 2016

High gradient magnetic separation (HGMS) is the most commonly used magnetic cell separation technique in biomedical science. However, parameters determining target cell capture efficiencies in HGMS are still not well understood. This limitation leads to loss of information and resources. The present study develops a bead-capture theory to predict capture efficiencies in HGMS. The theory is tested with CD3- and CD14-positive cells in combination with paramagnetic beads of different sizes and a generic immunomagnetic separation system. Data depict a linear relationship between normalized capture efficiency and the bead concentration. In addition, it is shown that key biological functions of target cells are not affected for all bead sizes and concentrations used. In summary, linear bead-capture theory predicts capture efficiency ($E_t$) in a highly significant manner.

• KSBB Journal
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• 제16권5호
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• pp.523-525
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• 2001

커들란은 용액의 pH 7.0에서 salt 상태로 존재하기 때문에 커들란만이 액상에 녹아 있는 경우에는 분리공정은 원심분리를 함으로써 분리가 가능하다. 그러나, 세포외 다당류로 생산된 커들란읜 세포, 염 의 무기물이 섞여 있기 때문에 이러한 물질을 제거하는 것이 커들란 분리의 중요한 변수가 되었다. 커들란의 생산을 위한 분리에 관한 보고는 원심분리 방법을 이용한 것으로 알려져 있음 세포 및 불순물 등을 제거하여 순수한 커들란을 분리하기 위해 pH swing separation (pH가 높은 경우 커들란이 용액에 녹는 성질을 이용)을 이용하였으나 원심분리를 수 차례 반복해야 되기 때문에 소요되는 동력이 많이 필요하여 에너지 절감형 및 오염절감형의 새로운 공정의 개발이 필요하게 되었다. 온 연구에서는 pH swing separation 에서 동력비가 많은 드는 단점을 감소시키기위하여 커들란의 물리 화학적인 특성을 고려한 분리공정을 개발하였다. 향후 공정 개선을 위한 지속적인 연구가 필요하며 완성시 경제적이 공정을 개발하는 것이 가능할 것이다.

• 대한수의학회지
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• 제29권2호
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• pp.33-39
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• 1989

Pure separation of various leukocytes is required for the assessment of their roles in immunological and phisiological function. In this study, pure separation of monocytes from canine peripheral blood was attempted. At first, mononuclear cells (PBMC) were separated by ficoll-hypaque gradient method and then monocytes were recovered from PBMC suspensions in sucrose gradient Sol. (PBMC-Sucrose), autologous plasma (PBMC-Plasma) and autologous serum (PBMC-Serum) incubated at $37^{\circ}C$ for 2 hours. 1. In the separation of PBMC by ficoll-hypaque gradient method in canine blood, higher relative centrifugal force (RCF) was required, as high as more than 1,300xg RCF for 40 minutes, for clear formation of PBMC layer than that in human blood as usually used 400xg RCF for 40 minutes. 2. In monocytes-separation from three PBMC suspensions following PBMC separation, recovery-, purity- and viability-rate of monocytes showed better results in PBMC-Plasma and PBMC-Serum than in PBMC-Sucrose suspension, particulary showing better results from PBMC suspensions performed by centrifugation at 1,500xg RCF for 40 minutes.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 The 6th International Symposium of East Asian Resources Recycling Technology
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• pp.770-775
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• 2001

Effects of magnetic field and carrier gas velocity on the magnetic separation of FCC catalyst by a high gradient magnetic separator were studied. The activities of the equilibrium catalyst, the magnetic particles and the nonmagnetic particles were evaluated in a fixed bed microreactor The results showed that heavy metal contaminated catalyst can be selectively separated by means of high gradient magnetic separation at magnetic field 0.5T and carrier gas velocity 0.3m.s$^{-1}$ , and lightly metal contaminated catalyst retained high catalytic activity.

• 한국초전도ㆍ저온공학회논문지
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• 제24권3호
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• pp.7-11
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• 2022

We are conducting research and development of magnetic filters for magnetic separation targeting paramagnetic materials. In order to develop a new magnetic filter with a large magnetic gradient, stainless fiber (SUS430, 120 mm × 3 mm) with a triangular cross section was sintered with a high void ratio (~ 70%) and the magnetic filter (20 mm × 2 mm) was created. When this magnetic filter was used to perform magnetic separation of hematite (particle size 50 ㎛) under a maximum magnetic flux density of 1.49 T, high separation rates were obtained.

• Journal of the Optical Society of Korea
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• 제20권1호
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• pp.118-124
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• 2016

The shape and location of the cutoff shield in a projection-type headlight system were optimized by a ray-tracing technique. A shield based on a Petsval surface showed better cutoff characteristics than a flat or cylindrical shield, such as a sharp intensity gradient and less color separation at the cutoff line. Adjustment of the shield’s location between the reflector and the aspheric lens further improved its cutoff characteristics.

• The Journal of the Acoustical Society of Korea
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• 제24권2E호
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• pp.54-60
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• 2005

A new multichannel blind deconvolution algorithm is proposed for speech mixtures. It employs unidirectional filters and normalization of gradient terms in the frequency domain. The proposed algorithm is shown to be approximately nonholonomic. Thus it provides improved convergence and separation performances without whitening effect for nonstationary sources such as speech and audio signals. Simulations using real world recordings confirm superior performances over existing algorithms and its usefulness for real applications.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2002년도 봄 학술발표논문집 Vol.29 No.1 (B)
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• pp.289-291
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• 2002

Host of source separation methods focus on stationary sources so higher-order statistics is necessary In this paler we consider a problem of source separation when sources are second-order nonstationary stochastic processes . We employ the natural gradient method and develop learning algorithms for both 1inear feedback and feedforward neural networks. Thus our algorithms possess equivariant property Local stabi1iffy analysis shows that separating solutions are always locally stable stationary points of the proposed algorithms, regardless of probability distributions of

• Journal of Microbiology and Biotechnology
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• 제9권4호
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• pp.522-524
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• 1999

A discontinuous Percoll gradient was used to separate islets from the collagenase-treated mouse pancreas easily and rapidly. Since the osmolality of Percoll is very low, adjustment of its osmolality to 340 mOs/kg $H_2$O was essential for securing the optimal separation. A discontinuous gradient layering with Percoll solution of 1.09 g, 1.07g, and1.05g/m, respectively, when centrifuged at 800$\times$g for 10 min, resulted in an optimal condition for separation and yielded a banding pattern with an even distribution of islet cells. No significant difference was observed in the morphological features between the Percoll-isolated and the manually-isolated islets. In conclusion, the discontinuous Percoll gradient can be effectively used to isolate the pancreatic islets from mice with four-fold higher efficiency compared to the handpicking method.

• 공업화학
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• 제8권4호
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• pp.694-699
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• 1997

대두에 포함된 유용 인지질 PE, PI, PC를 분리하기 위해서 정상 HPLC를 이용하였다. 이동상으로 hexane, isopropanol, methanol을 사용하였고 일정용매조성법으로는 세 물질을 분리할 수 없었기 때문에 구배용매조성법을 적용하였다. 최적 분리조건을 구하기 위해서 단 이론과 step-gradient mode에서의 용량인자를 계산하는 식으로부터 농도분포곡선을 계산하였다. 계산 결과에 의하면 PE는 일정용매조성법 (hexane/isopropanol/methanol=90/5/5vo1.%)에서 분리하고 PI와 PC를 분리하기 위해서는 10분의 구배시간과 두번째 이동상을 hexane/isopropanol/methanol=50/20/30(vol.%)으로 계단함수의 구배용매조성법을 사용하는 것이다. 계산된 최적 분리조건을 실험 data와 비교한 결과 잘 일치하였고 이 제시된 방법은 구배용매조성법에서 최적 분리조건을 구하는데 이용될 수 있었다.