• Natural Product Sciences
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• v.19 no.3
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• pp.236-241
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• 2013

A high-performance liquid chromatography-diode array detector (HPLC-DAD) method was established for quantitative evaluation of nine constituents of Fraxinus rhynchophylla such as four coumarins, esculin (1), fraxin (2), esculetin (3), fraxetin (4), three lignans, syringaresinol 4,4'-O-${\beta}$-diglucoside (5), pinoresinol 4-O-${\beta}$-glucoside (6), pinoresinol (9), one secoiridoid, oleuropein (7), and one coumarinolignan, cleomiscosin C (8). The preferred chromatographic condition was obtained on Phenomenex Gemini-NX (3 ${\mu}m$, C18 110A, $150{\times}4.60$ mm) and the mobile phase was composed of water and acetonitrile using a gradient elution. The wavelength was set at 220 nm. Extraction condition of these constituents in F. rhynchophylla was also optimized through extraction time, extraction solvent and extraction method using established method. From this study, extraction at $70^{\circ}C$ with the mixture of ethanol and water for more than 12 h was suggested to be good extraction condition for these constituents. Quantitation of nine constituents in different F. rhynchophylla samples was also successfully accomplished with the newly established method.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.153-158
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• 2023

Pinus species are native to the Northern Hemisphere and some parts of the tropics to temperate regions in the Southern Hemisphere. They were used as food and medicine in prehistoric times. Massonianoside B is a compound found in pine trees and possesses antioxidant activity. In order to determine the presence and content of this compound in Pinus species, three different parts (needles, branches, and bark) of three Pinus species were extracted and investigated. High-performance liquid chromatography with a gradient elution system along with a reverse-phase INNO column with photodiode array detector was employed. Results showed that the branches of the three Pinus species had higher massonianoside B content (5.502 to 9.751 mg/g DW) than either the needles or bark. Furthermore, among the three species, P. rigida × P. taeda had the highest concentration of total massonianoside B (11.557 mg/g DW). These findings thus provide evidence of biological activity in Pinus species and establish a foundation for further research.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.67-72
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• 2023

A simple and accurate method was developed for the quantitative analysis of oleanonic acid (OA) from mastic gum. The analysis was carried out using reverse-phase high-performance liquid chromatography combined with a photodiode array detector (HPLC/PDA). Our optimized method was validated by measuring various parameters, using an INNO C18 column fitted with a gradient elution system. The results revealed limits of detection and quantification of 0.34 and 1.042 ㎍/mL, respectively. The OA calibration curve exhibited excellent linearity over the concentration range of 0.0625 to 2.0 mg/mL, with r² =0.9996. Accuracy tests revealed a high recovery rate of 99.44-103.66%, with precision values below 0.15%. These results suggest that the present analytical method can identify and quantify OA in mastic gum with high precision. The HPLC approach developed in this study might be applied to routine analyses and large-scale extraction procedures for OA content quantification.

• Journal of IKEEE
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• v.16 no.4
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• pp.328-334
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• 2012

In this paper, we analyzed the electromagnetic characteristics of the spherical frequency selective surface with different arrangement of crossed dipole slot elements for reducing the RCS(radar cross section). The three dimensional MOM(method of moment) with RWG basis is used to analyze the proposed structure. To reduce the simulation time, we applied the BiCGSTab(Biconjugate Gradient Stabilized) algorithm as an iterative method and presented the comparison results with Mie's theoretical results for PEC sphere to show the validity of this paper. From the simulation results, the different arrangement of elements array showed the difference RCS that cannot be negligible. The arrangement method of element in frequency selective surface will be one of variables for the design of curved frequency selective structures.

• The Bulletin of The Korean Astronomical Society
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• v.45 no.1
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• pp.41.2-42
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• 2020

Understanding how the filamentary structure plays a role in the formation of the prestellar cores and stars is a key issue to challenge. We have observed two prestellar cores in surrounding filamentary environments in 13CO, C180 (3-2) and HCO+ (4-3) molecular lines with the Heterodyne Array Receiver Program (HARP) of the James Clerk Maxwell Telescope (JCMT), in order to search for the evidence related to the possible flow motions along the filament and/or the radial accretion (or infalling motions) of gas material toward the dense cores from their surrounding filamentary cloud. In L1544, the velocity gradient of 1.6 km s-1 pc-1 toward the core was measured in a small branch of filament lying on a radial direction of main filament while no velocity gradient along the main axis of filament in both 13CO and C18O lines. In L694-2, we found the velocity gradient of 0.6 km s-1 pc-1 along the filament in only 13CO lines. The projected accretion rate of ~6 M◉ Myr-1 was estimated in both cases. The infall (or radially contracting) velocity of gas material was measured ~0.16 km s-1 in both 13CO and HCO+ lines and in both L1544 and L694-2, which leads to estimate a mass infall rate of ~20 M◉ Myr-1. Our analysis suggests that our targets are at a stage where the gravitational contraction dominates the mass accretion through the surrounding filamentary cloud. This is consistent with the fact that our targets are highly evolved prestellar cores on a verge of star formation. More detailed results will be presented at the meeting.

• Analytical Science and Technology
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• v.19 no.6
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• pp.519-528
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• 2006

An analytical method for the determination of thyroid hormones in urine samples has been studied by using solid-phase extraction and high-performance liquid chromatography/diode array detector/electro-spray mass spectrometry. Seven thyroid hormones were successfully separated by gradient elution on the reverse phase Hypersil ODS column (4.6 mm I.D., 100 mm length, particle size $5{\mu}m$) with ammonium formate buffer and acetonitrile, and UV spectra and mass fragment could be confirmed. The extraction recoveries of thyroid hormones in the urine samples (pH 3) were in the range of 89.0-113.1% with solid-phase extraction by C18, followed by elution with 4 ml of methanol/ammonium hydroxide (9 : 1). The calibration curves showed good linearity with the correlation coefficients ($r^2$) varying from 0.992 to 0.998 and the detection limits of all analytes were obtained in the range of 2-4 ng/ml (3.8-13.0 pmol/ml).

• Proceedings of the KIPE Conference
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• 2016.07a
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• pp.183-184
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• 2016

태양광 패널 등가모델을 결합한 태양광 발전 패널 모의 장치(SAS)는 정확도 면에서 많은 장점이 있다. 특히 SAS에서 사용되는 등가회로 모델은 주변 환경에 맞게 빠르게 변하는 I-V 출력특성을 추출할 수 있어야 하며, 추출시간이 짧을수록 좋다. 본 논문은 태양광 등가회로 모델의 파라미터를 빠르게 추출하여 I-V 특성곡선을 모사할 수 있는 방법을 제안한다. 제안한 모델은 실시간으로 변화하는 태양광 패널의 출력 특성을 빠르게 모사하기 위해 미분을 사용해서 등가회로의 파라미터를 추출한다. 제안 모델의 타당성 검증은 기존의 사용하는 방법들과의 비교를 통해 진행하였고, 그 결과 정확도는 기존의 사용하는 방법과 비슷하게 유지하면서 속도는 향상됨을 확인할 수 있었다.

• Proceedings of the Korea Electromagnetic Engineering Society Conference
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• 2005.11a
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• pp.153-156
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• 2005

This paper concerns an efficient iterative approach for eliminating coherent interference signals in linearly constrained adaptive arrays. The Alternate Mainbeam Nulling Algorithm[1] is implemented iteratively to find an optimum weight vector. The convergence parameters in the unit gain and null constraints are calculated using steepest descent method with gradient estimation. The nulling performance of the proposed method is compared with that of conventional ones. It is shown that the proposed method performs better than conventional ones when the power of the coherent signals is large compared with a desired signal. Also, it performs consistently well for more number of interferences.

• Korean Journal of Mathematics
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• v.15 no.2
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• pp.195-206
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• 2007

We investigate the multiplicity of $2{\pi}$-periodic solutions of the nonlinear Hamiltonian system with perturbed polynomial and exponential potentials, $\dot{z}= JG^{\prime}(z)$, where $z:R{\rightarrow}R^{2n}$, $\dot{z}={\frac{dz}{dt}}$, $J=\(\array{0&-I\\I&0}\)$, I is the identity matrix on $R^n,G:R^{2n}{\rightarrow}R$, G(0, 0) = 0 and $G^{\prime}$ is the gradient of G. We look for the weak solutions $z=(p,q){\in}E$ of the nonlinear Hamiltonian system.

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.279.2-280
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• 2003

A simple and accurate reverse-phase high performance liquid chromatography (HPLC) coupled with photodiode array was developed for the determination of dextromethorphan(DM) and its metabolite dextrorphan(DX) in human urine. Chromatographic separation was accomplished on a cyano analytical column at 220 nm using a mobile phase containing 25 mM triethylammonium phosphate buffer(PH 3.0) in a 0-70％ ACN gradient and triazolam(TZ) was used as internal standard(I.S). (omitted)