• 제목/요약/키워드: Grace

검색결과 334건 처리시간 0.022초

Nutritional Studies on Production of Antibacterial Activity by the Zebra Mussel Antagonist, Pseudomonas fluorescens CL0145A

  • Polanski-Cordovano, Grace;Romano, Lea;Marotta, Lauren L.C.;Jacob, Serena;Hoo, Jennifer Soo;Tartaglia, Elena;Asokan, Deepa;Kar, Simkie;Demain, Arnold L.
    • Journal of Microbiology and Biotechnology
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    • 제23권5호
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    • pp.656-660
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    • 2013
  • Pseudomonas fluorescens strain CL0145A was discovered at the New York State Museum Field Research Laboratory as an effective agent against the environmentally destructive zebra mussel, which has contaminated US waters. Dried cells of the microbe are being commercialized as an environmentally friendly solution to the problem. We found that antibiotic activity against the Gram-positive bacterium Bacillus subtilis is produced and excreted by this strain. We have carried out studies to optimize production of the antibiotic. Studies were begun in a complex corn meal medium. Activity was found in both cells and culture supernates and was maximal after one day of fermentation. Static fermentation conditions were found to be superior to shaken culture. Production of extracellular antibiotic in complex medium was found to be dependent on the content of sucrose and enzyme-hydrolyzed casein. Indeed, production was greater in sucrose plus enzyme-hydrolyzed casein than in the complex medium. Of a large number of carbon sources studied as improvements over sucrose, the best was glycerol. An examination of nitrogen sources showed that production was improved by replacement of enzyme-hydrolyzed casein with soy hydrolysates. Production in the simple glycerol-Hy-Soy medium was not improved by addition of an inorganic salt mixture or by complex nitrogen sources, with the exception of malt extract. In an attempt to keep the medium more defined, we studied the effect of amino acids and vitamins as replacements for malt extract. Of 21 amino acids and 7 vitamins, we found tryptophan, glutamine, biotin, and riboflavin to be stimulatory. The final medium contained glycerol, Hy-Soy, tryptophan, glutamine, biotin, and riboflavin.

일 지역 노인의 건강관련 삶의 질 (Health Related Quality of Life of The Elderly in a Small and Medium-Sized City in Rural Area)

  • 이혜경;이창금;김경수
    • 디지털융복합연구
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    • 제16권6호
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    • pp.343-352
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    • 2018
  • 본 연구의 목적은 일개 중소도시 지방 65세 이상 노인을 대상으로 건강관련 삶의 질과 이에 영향을 미치는 요인을 분석하여 중소도시 노인의 삶의 질 향상을 위한 방안을 마련하기 위하여 시도 되었다. 조사대상은 2015년 9월 21일부터 10월 30일까지 자료를 수집하였고, spss/win 23.0을 이용하여 분석하였다. 연구결과 대상자의 일반적 특성과 건강관련 삶의 질은 성별(t=2.36, p=.019), 사회참여(t=4.10, p=.000), 주관적 건강상태(F=12.08, p<.001), 의료비부담 수준(F=4.39, p=.014), 주관적 경제상태(F=3.50, p=.032), 만성질환 수(F=7.91, p<.001)에 따라 유의한 차이가 있었다. 대상자의 건강관련 삶의 질의 영향요인은 사회참여, 성별, 주관적 건강상태, 가족지지, 의료비부담 순으로 나타났으며 설명력은 26.3%이었다. 따라서 중소도시 65세 이상 노인을 대상으로 건강관련 삶의 질 향상을 위하여 사회 참여, 성별, 주관적 건강상태, 가족지지, 의료비 부담 등을 고려한 지역적 특성에 맞는 실제적이고 포괄적이며 지속적인 건강증진프로그램 개발이 필요하다.

대도시 가족의 주거생활양식에 관한 연구 I - 주거생활양식 유형화를 중심으로 - (A Study on the Housing Life Style of Families Living in Metropolitan Areas I: with special reference to patterning of Housing Life style)

  • 이연복;홍형옥
    • 대한가정학회지
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    • 제38권1호
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    • pp.75-87
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    • 2000
  • The aims of this study are to establish a mode of housing life style that meet the demands of families living in big cities, and the contribute to the development of a better housing life style pattern by considering metropolitan residents' choice of housing and basic concepts of their behavioral patterns. The results of this study were as follows: 1. Value orientation of family life was divided into value orientation of family and value orientation of housing. Value orientation of family was constituted of four factors such as 'harmony', 'leisure and hobbies', 'individual development and its support' , 'education' . Value orientation of housing is constituted of three factors such as 'physical characteristics of house' , '\`environmental condition', 'socio-economic conditions of housing'. The comprehensive life values were constituted of six factors such as 'the importance of communal family', 'the importance of housing decoration and housing life benefits', 'the importance of security and holding environment' , 'the importance of familial harmony', 'the importance of privacy', 'the importance of convenience and natural environment'. Based on three factors, were found to be fine types of value orientation of family life which were 'pursue healthy of family', 'comfort of family', 'clean environment', 'convenient environment', and 'harmonious relationship among family members'. Variables influencing the value orientation of family life were property and price of housing. 2. Consumption propensity when buying house, furniture and durables were constituted of four factors which were propensity to 'beauty', 'fashion', 'tradition and symbol', and 'pragmatic use'. Based on these factors, there were found to be four types of consumption propensity when buying house, furniture and durables which were 'fashion', 'tradition and symbol', 'beauty', and 'pragmatic use'. Variables influencing spending habits were found to be objective social class (SES), types of residence, wife's educational background, and price of housing. 3. Propensity to using space were constituted of three factors which were 'convenience', 'politeness and social grace', and 'housekeeping'. Based on these factors, there were found to be three types of propensity to using space which were 'individuality', 'convenience', and 'politeness and housekeeping'. Variables influencing propensity to use of space were found to be objective social class (SES), wife's educational background, types of homeownership and price of housing 4. According to this study, there were found to be six patterns of housing life style: 1) family that seeks formalist life, 2) family that seeks harmonious life, 3) family that seeks active healthy life, 4) family that combine various factors, 5) family that seeks convenience, 6) family that stresses environment.

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의복문양(衣服紋樣)의 기호도에 관한 실태(實態) 연구(硏究) - 한복(韓服)을 중심(中心)으로 - (A Study on Present Status of Preferences in the Choice of Patterns in Clothing)

  • 김은주
    • 복식
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    • 제6권
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    • pp.65-87
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    • 1982
  • 1. Purpose: The purpose is to apply and adapt ancient textile pattern to practical life, and to contribute to the promotion of the creative development of modernization and Koreanization of textile pattern by the survey of the extent of taste for the textile pattern existent in Korean costume among costumes. 2. Hypothesis: Textile pattern is in most classical one expressed in fact, but nowadays abstract or geometric one is more exist. Consumers are in most part in forties, and so the taste cannot be ignored. Therefore aesthetic consciousness found Korean costume is more important rather than excessively bold or mordern one. 3. Method and Process: (1) This research is analysed 386 one through college student and parents of a primary or high school. (2) Taste of the pattern is comparisoned and analyzed as age. education, pocket money of the student or an income of a month in home through computer. And the analysis of the statistics research is operation through the Chi-Square Goodness of-Fit-Test. 4. Conclusion: (1) The Korean costume put on mostly party or formal dress, therefore it should be researched as an outdoor dress with traditional costume. (2) The most one is concerned in textile pattern, it's taste is different from color, constitution. Textile industry is developed not only weave pattern, gold-coverning pattern but embroidry pattern, dye pattern, therefore the various side of pattern design should be researched. (3) In Side of form, plant pattern is not only most various but highest in popularity. According to approach nowadays, all of the pattern becomes composition or geometric one with abstract tendency and so from these day pattern is necessary combinationed, researched with our people emotion. (4) The color of textile pattern is choiced individual taste. Through investige, research in various side of the modern taste and our costume, we take growing interest in the color, composition of textile, and should dressed with beauty and grace. (5) In side of size, textile pattern is choiced to individual face and body shape through research on form, color, size of these pattern, we should endeavor to dress well. (6) In side of application, most people is concerned in not only interior ornament but the costume is high. Costume research by some people of the outside and symbol with interior through the pattern analysis is not only very important but also is due to people culture.

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하브루타 수업이 고등학생의 지리학습에 미치는 영향 (The Effects of Havruta Class on High School Students' Geographical Attitude and Academic Achievement)

  • 강은정;조철기
    • 한국지역지리학회지
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    • 제23권2호
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    • pp.420-436
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    • 2017
  • 이 연구는 지리수업에 하브루타 학습방법을 적용하여 그 효과를 검증한 것이다. 하브루타 수업을 적용한 후 설문조사 및 심층 인터뷰를 실시하여 얻은 결과는 다음과 같다. 지리수업에 대한 학생들의 태도에 긍정적인 변화를 가져오는 등 유의미한 결과를 얻었다. 하브루타를 적용한 지리수업에 대한 학생들의 흥미 및 참여도에 있어서 효과적이었다. 뿐만 아니라 학생들의 수업 이해도, 문제해결력 및 사고력 향상에도 효과적임을 알 수 있었다. 학생들은 활발한 대화와 질문을 통한 상호작용의 결과로 자신의 생각을 표현하고 설명해 주는 하브루타 활동으로 지리수업에 대해 흥미를 느끼고 보다 적극적이고 능동적인 자세로 수업에 참여한 것으로 나타났다. 그러나 하브루타 수업을 적용한 수업 이후 학생들의 학업성취도 변화를 통계처리한 결과, 유의미한 차이는 얻지 못했다. 이 연구는 4차시의 짧은 기간 학생들을 대상으로 한 단원의 내용에 대한 효과를 검증하였으므로 하브루타를 적용한 수업의 효과를 검증하는데 한계가 있는 것으로 판단된다. 단기간의 하브루타 수업이후 학업성취도의 효과를 기대하기보다 학습 태도와 같은 정의적 영역에 미치는 영향이 더욱 크다는 것을 알 수 있었다.

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Effects of replacing fish oil with palm oil in diets of Nile tilapia (Oreochromis niloticus) on muscle biochemical composition, enzyme activities, and mRNA expression of growth-related genes

  • Ayisi, Christian Larbi;Zhao, Jinliang;Yame, Chen;Apraku, Andrews;Debra, Grace
    • Fisheries and Aquatic Sciences
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    • 제22권11호
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    • pp.25.1-25.9
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    • 2019
  • Background: Due to the continuous demand for fish coupled with decline in capture fisheries, there is the need to increase aquaculture production to meet the demand. Aquaculture is faced with high cost of feeding since fish oil and fish meal are expensive. In view of this, there are calls to explore alternatives that are cheap and reliable. Objectives: This study on Oreochromis niloticus was conducted to evaluate the effects of replacing fish oil (FO) with palm oil (PO) at 0%, 25%, 50%, 75%, and 100% on muscle fatty acid and proximate composition as well as growthrelated enzyme activities and mRNA expression. Methods: Oreochromis niloticus were fed five experimental diets (33% crude protein and 10% crude lipid) for 8 weeks. Feed had variation in fish oil and palm oil contents. After the 8 weeks feeding trial, five fish were sampled from each tank (15 from each treatment) and euthanized using an excess dose of tricaine methane sulfonate (MS-222 at 200 mg/L). Fatty acid and enzyme activities were analyzed using standard protocols. Also, RT-qPCR was used to quantify the expression levels of selected growth-related genes. Results: Fish fed 25% PO recorded the least muscle protein content and was significantly lower than the group fed 100% PO. Paired box protein 7 (Pax-7) enzyme activity was significantly higher in the group fed 50% PO compared to the groups fed 25% PO and 100% PO, while caplain-3 (Capn-3) was significantly lower in the group fed 0% PO compared to all other groups. There was a significant difference among treatments with respect to mRNA expression of Pax-7 and Capn-3. Group fed 25% PO had significantly lower mRNA expression of Pax-7, while the group fed 75% PO recorded significantly higher mRNA expression of Capn-3 compared to groups fed 0% PO, 25% PO, and 100% PO. Pearson's correlation analysis revealed that Igf-I and Igf-II mRNA expression have significant correlation with n-3 polyunsaturated fatty acids content in muscle. Conclusion: The results suggest muscle protein content could be modified if FO is replaced with PO. Also, mRNA expression of Pax-7 and Capn-3 is affected by replacing FO with PO.

바이오플라스틱 생산 미생물 플랫폼 제작을 위한 대사공학 전략 개발 (Development of Metabolic Engineering Strategies for Microbial Platform to Produce Bioplastics)

  • 박시재;요키미코 데이비드;메리 그레이스 베일런;홍순호;오영훈;양정은;최소영;이승환;이상엽
    • 공업화학
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    • 제25권2호
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    • pp.134-141
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    • 2014
  • 환경오염, 기후변화, 고갈되어가는 화석원료에 대한 문제를 해결하기 위해 재생가능한 자원으로부터 케미칼 및 고분자 등의 산업자원을 생산하는 친환경 공정개발에 많은 연구가 진행되고 있다. 최근에 재생가능한 바이오매스로부터 다양한 케미칼 및 고분자 등을 생산하는 바이오리파이너리 공정이 많은 관심을 받고 있으며, 석유화학기반산업을 보완 혹은 대체할 가능성이 매우 높은 친환경공정으로 생각되고 있다. 본 총설에서는 바이오리파이너리 공정에 핵심적인 촉매로 사용되고 있는 재조합 미생물의 개발의 최근 동향을 바이오나일론, 바이오폴리에스터의 생산을 위하여 개발되고 있는 재조합 미생물의 대사공학전략을 중심으로 살펴보고자 한다.

개의 후복강과 골반강에 발생한 지방종의 진단영상 증례 (Diagnostic Imaging of Lipoma in the Retroperitoneum and Pelvic Cavity in a Dog)

  • 이남순;김준영;나응식;김미은;이혜연;최미현;김완희;김대용;최민철;윤정희
    • 한국임상수의학회지
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    • 제27권1호
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    • pp.88-92
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    • 2010
  • 11살의 중성화 암컷 miniature Schnauzer 견이 식욕부진, 복부팽만, 빈뇨증상과 변비증상으로 내원하였다. 복부 일반 방사선 검사에서 하나의 큰 균질한 지방 밀도의 종괴가 후복강과 골반강에서 확인되었다. 그 종괴는 내림결장을 변연부와 배쪽으로, 방광을 배쪽으로 변위시켰다. 복부 초음파 검사에서 종괴는 내부의 거친 에코구조와 바깥쪽에 고에코성 막을 갖고 있으며, 컴퓨터단층촬영에서 균질하게 주위 지방과 같은 (-180 ~ -110 HU) 감쇄성을 보였다. 주위 조직으로의 침습의 증거는 없었다. 세침흡인을 통한 세포검사 결과는 많은 층과 군집의 지방세포들이었다. 환자는 수술적으로 종괴를 제거한 후 임상증상이 완전히 해소되었다. 조직병리학적 검사를 통해 그 종괴는 지방종으로 진단되었다.

Arshile Gorky와 Jackson Pollock의 Painting이 현대의상 직물 문양에 미친 영향 (The Influence of Arshile Gorky's & Jackson Pollock's Painting on Modern Fashion)

  • 정흥숙
    • 한국의류학회지
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    • 제16권3호
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    • pp.197-207
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    • 1992
  • Expressionism, is as diverse as the artists invo)ved, in a very broad sense two main tendencies may be noted. The first is that of the Action painters, concerned in different ways with the gesture of the brush and the texture of the paint. It included such major artists as Arshile Gorky, Jackson Pollock, Willem De Keening, and Franz Kline. The other group consisted of the Color Field painters, concerned with the statement of an abstract sign or tranquil image in terms of a large, unified color shape or area. Here must be included Mark Rothko, Barnett Newman, Ad Rdinhardt, as well as, to a degree, Adolph Gottlieb, Robert Motherwell, and Clyf(ord Still. In this paper, 1 selected two artists Arshile Gorky and Jackson Pollock independent charac-teristics and studied the influence of their Action painting on the fabrics of modern fashion. However, it should be noted it was never the intention of the critic Harold Rosenberg, in coining this term, to imply that Action painting was a kind of athletic exercise. Nor is it true that the furious and seemingly haphazard scattering of the paint involved a completely uncontrolled, intuitive act. There is no question that, in the paintings of Jackson Pollock, Arshile Gorky and many of the other Abstract Expressionists, the element of intuition or the accidental plays a large and deliberate part; this was indeed one of the principal contributions of Abstract Expressionism which had found its own inspiration in surrealism's 'psychic automaton'. However, nothing that an experienced and accomplished artist does can be completely accidental. Aside from their intrinsic quality, the spun-out skeins of poured pigments contributed other elements that changed the course of modern painting. There was the concept of the all-over painting, the painting seemingly without beginning or end, extending to the very limits of the canvas and implying an extension even beyond. The feeling of absorption or participation is heightened by the ambiguity of the picture space. The colors and lines, although never punctur-ing deep perspective holes in the surface, still create an illusion of continuous movement, a billowing, a surging back and forth, within a limited depth. To study the influence of Abstract Expressionism on the fabric of modern fashion, 1 selected and examined four fashion magazines: Collezioni published in France, Bazaar in Italy, Gap in Japan and Vogue in the U.S.a. froim January 1989 to June 1991. As a result of this review I found that some fabrics used in modern clothing are printed in a dripping, pouring and splashing style without any meaning or form. Slides included in the presentation show that modern fabrics which are printed in such a style were influenced by Abstract Expressionism. The slides also show that these abstract prints are well suited to modern fashion design.

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일본 출신 결혼이주여성이 인지하는 자녀에 대한 민족사회화 수행 : 만 7-18세 자녀를 중심으로 (Perceived Enactment of Ethnic Socialization by Japanese Marriage Migrant Mothers of 7- to 18-Year-Old Children)

  • 윤수현
    • 가정과삶의질연구
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    • 제33권3호
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    • pp.15-29
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    • 2015
  • The purpose of this study was to explore ethnic socialization enacted by Japanese marriage migrant women currently raising 7- to 18-year-old children in Korea. To accomplish this goal, we examined the following research questions: (1) What is the level of ethnic socialization enacted by Japanese marriage migrant mothers? (2) Does the level of ethnic socialization vary by demographic and ethnocultural factors? (3) To what extent do demographic and ethnocultural factors influence the enactment of ethnic socialization by Japanese marriage migrant mothers? The sample consisted of 243 Japanese marriage migrant women currently raising 7- to 18-year-old children in Korea. For data analysis, T-test, correlation, ANOVA, and hierarchical regression analyses were used. Also, we performed separate analyses for two subtypes of ethnic socialization in particular, namely cultural socialization and preparation for bias. Ethnocultural factors examined in this study were the level of discrimination experience, Japanese ethnic identity, and husbands' ethnic orientation in childrearing. The main results of this study were as follows. First, results showed that Japanese marriage migrant women enacted moderate levels of cultural socialization and preparation for bias. Second, Japanese marriage migrant women's cultural socialization and preparation for bias were both positively related to the levels of women's discrimination experience and Japanese ethnic identity. Also, the younger the age of the firstborn child, the more preparation for bias these women performed. In addition, women whose Korean husbands wanted to raise their children more biculturally performed more cultural socialization compared to those with Korean husbands who wanted to raise their children as Korean. Third, hierarchical regression analyses revealed that women's discrimination experience and ethnic identity positively predicted both types of ethnic socialization. Also, Korean husband's bicultural orientation towards childrearing predicted a higher level of cultural socialization by Japanese marriage migrant women. Results of this study provide basic information about ethnic socialization among multicultural families in Korea, which can be useful for promoting positive self-identity among multicultural children. Furthermore, the results suggest that husbands' support and cooperation in ethnic socialization can be crucial for marriage migrant women to socialize their children utilizing their cultural and experiential resources.