• 한국동물위생학회지
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• 제36권2호
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• pp.111-119
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• 2013

The aim of this study was to investigate the isolation frequency and antimicrobial resistance of Escherichia coli (E. coli) and Enterococcus spp. from domestic beef and pork on sale in Seoul, Korea. A total of 106 (10.4%) E. coli and 114 (11.2%) Enterococcus spp. from 635 domestic beef and 381 pork samples were isolated and examined for susceptibility to 15 and 11 antimicrobial agents, respectively. The most frequent antimicrobial resistance observed in E. coli isolates was to ampicillin (38.6%), followed by streptocmycin (34.9%) and tetracycline (32.0%). The most frequent antimicrobial resistance in E. faecium isolates was to erythromycin (53.8%) and rifampin (46.1%), and in E. faecalis isolates was to tetracycline (55.7%) and rifampin (55.0%). Among the isolates, multi-drug resistant (MDR) E. coli and Enterococcus spp. strains showing resistances to more than two antimicrobial agents tested were 10.4% and 11.2%, respectively. As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in future.

• 약학회지
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• 제54권1호
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• pp.39-48
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• 2010

The objective of this study was to analyze sennoside A and rhaponticin simultaneously according to sennoside A of Rhei Rhizoma determination in Korean Pharmacopoeia. $NaHCO_3$ solution in KP was compared with methanol which usually used as solution to extract rhaponticin in Rhei Rhizoma. The method was validated through the guidelines of linearity, LOD, LOQ, specificity and accuracy. Two solution weren't different about validation parameter and passed. So this method were applied to the determination of 6 commercial Rhei Rhizoma samples and 2 samples were suitable for the legal standards.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.297-303
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• 2020

Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities were investigated for comparison of the biological activity of the berry extracts. Chlorogenic acid was the major phenolic acid, which had the highest content in aronia, followed by blueberry. The rutin and isoquercetin contents were the highest in mulberry, followed by aronia, black raspberry, and blueberry. The anthocyanin content was the highest in black raspberry. Aronia showed the highest DPPH free radical and nitrite scavenging activities with the highest contents of total phenolics and proanthocyanidins.

• Journal of Applied Biological Chemistry
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• 제56권1호
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• pp.29-35
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• 2013

This study was performed to analyze 48 kinds of pesticide residues using gas chromatography (GC)/nitrogen phosphorous detector, GC/micro electron capture detector, GC/mass selective detector, and high performance liquid chromatograph/diode array detector in 186 fresh ginseng samples collected in the Seoul area from 2010 to 2011. Fresh ginseng dietary intakes were estimated using the data from the 2009 Korea National Health and Nutrition examination survey. Residual pesticides were detected in 79 samples (42.5%) with eight different fungicides. Only 20 samples (10.8%) exceeded the maximum residue limits (MRLs) for pesticides registered by the Korea Food & Drug Administration. Among them, tolclofos-methyl residues (10.2%) exceeded the MRL for fresh ginseng in 18 ginseng seedlings and one of the two-year old fresh ginseng plants, and the residual level in just one ginseng seedling violated the MRL for pyrimethanil. The results showed that residual pesticides levels in marketable fresh ginseng around Seoul were relatively safe. The percent acceptable daily intake (%ADI) was calculated using pesticide residues in fresh ginseng and dietary intakes of fresh ginseng. The risk caused by pesticide residues in fresh ginseng was very low.

• 한국식생활문화학회지
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• 제30권6호
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• pp.783-789
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• 2015

In recent years, consumers have become more interested in convenient lifestyles, leading to increased use of salted cabbages for preparation of kimchi. This study aimed to investigate the safety of heavy metals, pesticide residues, and foodborne pathogens in commercial salted cabbages in Seoul from August to November, 2014. The survey, which was conducted to determine whether or not salted cabbages were prepared under the highest sanitary conditions, showed that Seoulites are interested in purchasing hygienic and safe salted cabbages. The average amounts (range) of Pb and Cd found in 30 salted cabbage samples were 0.007 (0.000~0.063) mg/kg and 0.004 (0.000~0.012) mg/kg, respectively. The cabbages were analyzed for residues from 285 types of pesticides using the multiresidue method. Residues for pesticides were not detected. Major foodborne pathogens, specifically Escherichia coli, Salmonella spp., Listeria monocytogenes, Campylobacter jejuni, Staphylococcus aureus, Vibrio parahaemolyticus, Enterohemorrhagic Escherichia coli, Bacillus cereus, Clostridium perfringens and Norovirus, were also not detected.

• 생약학회지
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• 제47권4호
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• pp.374-380
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• 2016

The purpose of this study was to evaluate of quality variation of Sibujeondaebotang according to storage period and temperature (5 and $25^{\circ}C$) Identificaion test, pH, specific gravity, heavy metal, microbial limit test and glycyrrhizic acid, paeoniflorin content of Sibjeondaebotang were performed during 12 months. The significant change was not shown on identificaion test, specific gravity, microbial limit test under long term storage and the result suited a safe level standards of 'Korean National Standard of Traditional Medicinal (Herbal and Botanical) Materials - "Sibjeondaebotang"'. The test of pH showed meaningful changes between two storage temperature (5 and $25^{\circ}C$) according to increasing storage period. The contents of glycyrrhizic acid and paeoniflorin had no relation with storage temperature($5^{\circ}C$, $25^{\circ}C$) but it showed a tendency to decrease according to the length of storage period. We predicted shelf-life of glycyrrhizic acid and paeoniflorin at storgae temperature(5 and $25^{\circ}C$) as about 6 and 3 months, respecttively. Therefore considering glycyrrhizic acid and paeoniflorin, sibjeondaebotang is determined to be in good condition within 3 months, regardless of the room temperature and refrigerated temperature. We were expected to serve as a basis for the guideline of medication counseling sibjeondaebotang.

• 한국동물위생학회지
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• 제31권3호
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• pp.375-384
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• 2008

This study was performed to establish the discrimination of the corpus albicans that were formed after normal parturition and abortion in Holstein, The corpus albicans were obtained from 504 cows and 506 heifers slaughtered in the Agricultural Cooperative's Livestock Products Joint Market of Seoul City from March 2007 to October 2007. The difference in a gross examination (morphology, fibrogenesis, deposit state, color, and size) among the fixtures that exist in their ovaries was investigated. All of the cows had corpus albicans in their ovaries: 423 cows (83.9%) had less than three corpus albicans, 81 (16.1%) had more than four. In the heifers, however, 463 (91.05%) had no corpus albicans, 39 (7.7%) had one, and four (0.8%) had more than two. In the types of corpus albicans 73.2% of the cows had oval-type, 14.4% had circular-shaped, 12.4% had irregular-type. In the aborted heifers, however, oval-type appeared in 88.6% and circular-shaped appeared in 11.4%, and no irregular-type was found. There was no significant difference in the fibrogenesis and size of corpus albicans between cows and aborted heifers. However, slight difference in the color of the corpus albicans was observed between two types. In conclusion, it is not easy to discriminate by gross examination of ovary between cows and aborted heifers in the slaughter house.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.381-381
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• 2010

2010년 5월 중 서울 약령시와 서울시내 대형마트에서 유통 중인 약용버섯 및 식용버섯류 16품목 92건 대상으로 중금속(비소, 카드뮴, 납, 수은) 모니터링을 실시하였다. 식품의약품안전청에서 고시한 생약의 잔류오염물질 허용기준 및 시험방법에 따라 실험하였으며, 유통 버섯류의 중금속 함유실태를 파악하고, 버섯의 안전성 기준 제정을 위한 기초 자료를 제공하고자 본 실험을 실시하였다. 새송이버섯 등 생버섯 7종 15건에 대한 중금속 검사 결과 비소 0.01~0.26 mg/kg, 카드뮴 0~0.10 mg/kg, 납 0~0.03 mg/kg, 수은 0~0.007 mg/kg 으로 중금속 함유량이 낮았다. 상황버섯 등 11종 77건의 건조되어 식용 및 약용으로 사용되는 버섯류 중 노루궁뎅이버섯은 4건 중 2건(50.0 %)에서 카드뮴이 0.32~0.82 mg/kg으로 한약재 기준 대비 높게 검출되었으며, 상황버섯은 15건 중 5건(33 %)에서 카드뮴 0.58~0.85 mg/kg, 표고버섯은 9건 중 3건에서 카드뮴 0.42~0.74 mg/kg, 운지버섯 5건 중 1건(20.0 %)에서 1.66 mg/kg, 영지버섯 16건 중 2건(12.5%)에서 수은 0.415 mg/kg과 카드뮴 0.66 mg/kg이 분석되었다. 특히 아가리쿠스버섯은 10건 중 10건(100.0 %)에서 카드뮴(10건) 1.49~7.35 mg/kg, 수은(9건) 0.202~0.505 mg/kg으로 높은 유해중금속 함유량을 보이는 것으로 조사되었다.

• 분석과학
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• 제29권6호
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• pp.249-254
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• 2016

서울지역 지하철역사내 자판기에서 판매되는 음료현황 및 설문조사 결과, 자판기 진열음료 중 탄산음료의 비율이 36%으로 가장 높았고, 설문응답자들은 갈증해소를 위해 당류 함량이 많은 탄산음료와 이온음료를 구매하였다. 또한 다빈도 진열음료에 대한 당류 함량 분석결과, 탄산음료 1회 제공량에는 평균 30.4 g, 최고 50.1 g 으로 각설탕(3 g)으로는 평균 10개에서 최고 17개 분량의 당류가 있었다. 이는 세계보건기구의 1일 당류 섭취 권고기준 50 g의 평균 60 % 수준이었고, 일부 탄산음료는 한 캔으로도 권고기준을 모두 섭취하는 제품도 있었다.

• 한국식품위생안전성학회지
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• 제29권3호
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• pp.241-247
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• 2014

In this study, HPLC-RI was used to determine sugars and sugar alcohols contents in 102 different kinds of processed foods met to children's taste. The average amount of sugars per 100 g of candies was 70.25 g, of processed cocoa products was 65.34 g, of processed chocolate products was 47.53 g, of breads was 25.66 g, of cookies was 22.28 g, of ices was 12.47 g, of snacks was 9.74 g. Processed cocoa product items had relatively higher sucrose contents (56.80 g/100 g) than any other items. The average amount of sucrose per 100 g of candies was 44.20 g, of processed chocolate products was 32.89 g, of breads was 23.11 g. When the contents of sugars in processed foods met to children's taste per on serving size were compared to WHO guidelines, the percentages were 5.84~28.52 about recommended daily intake of total sugar of 50 g. The result for the analysis of confectioneries showed that 13 samples of 102 were detected and the sugar alcohols content of samples investigated varied between 0.01~15.06%.