• 생약학회지
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• 제50권4호
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• pp.318-323
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• 2019

This study was designed to establish the stability of EGHB010, a combined hot water extract of Paeoniae Radix and Glycyrrhizae Radix. Test of coliform groups, sensory evaluations, contents of moisture, and the amount of paeoniflorin and glycyrrhizin were determined during storage at 4, 25 and 35℃ for 6 months. Coliform groups were not observed and moisture contents were maintained about 3.0% during the storage period. Sensory characteristics including color, taste, texture and appearance were tested over 3 points throughout the entire storage period. It maintained the commercial value during the storage period. Contents of paeoniflorin and glycyrrhizin as indicator components were remained stably for the storage period. The shelf-life of EGHB010 based on a linear response to zero of sensory evaluation with the highest correlation coefficient was predicted. Finally, the shelf-life of EGHB010 was expected for about 3 years to consider in safety coefficient. The shelf-life data of EGHB010 can be used to develop a functional health ingredient.

• 동의생리병리학회지
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• 제16권3호
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• pp.499-506
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• 2002

Two of the essential processes required for metastasis are neoangiogenesis and tumor cell invasion of basement membranes (BM) and extracellular matrix (ECM). Recently, data showed that herbs removing blood stasis has an anti-angiogenic effects. Tonifying vital Qi and eliminating pathogenic factor was a basic modality in Oriental oncology. In this study, we investigated several Qi and Blood tonics for potent angiogenic inhibitors. Methanol extracts of samples inhibited the proliferation of ECV-304 at the concentration of 100 ㎍/㎖. Zizyphi Fructus, Glycyrrhizae Radix, Angelicae Gigantis Radix decreased the gelatinolytic activity of MMP-9 from ECV-3Q4, at the concentration of 100 ㎍/㎖ in gelatin zymography. In in vitro invasion assay, herbs inhibited the invasion activity of ECV-304 by 53% of control (Ginseng Radix), 39% (Zizyphi Fructus), 36% (Angelicae Gigantis Radix), 25% (Glycyrrhizae Radix). Ginseng Radix inhibited the capillary-like tube formation of ECV-304 at the concentration of 160 ㎍/㎖, Angelicae Gigantis Radix and Paeoniae Radix Alba inhibited at the concentration of 320 ㎍/㎖. These results indicated that Ginseng Radix, Glycyrrhizae Radix, and Angelicae Gigantis Radix could be considered as potent angiogenic inhibitiors.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.126-126
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• 2018

Many of researches have revealed that human intestinal microbiota is related to health. Several diseases like obesity, diabetes, and hypertension are affected by the microbiota directly and indirectly. So, interventions with food and drug have been tried to change a composition of the microbiota to better condition. However, few natural medicines have elucidated to date. To understand an influence on microbiota by plant materials including Glycyrrhizae Radix, the extract of medicines were administered to mice and the feces were collected before and after the administration. The feces were analyzed by terminal restriction fragment length polymorphism (T-RFLP). The changes in composition of mice gut microbiota were detected and analyzed. The data could be utilized to further study about biological activities of the plant medicines.

• 한국임상수의학회지
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• 제22권4호
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• pp.353-356
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• 2005

This study was performed to examine the antibacterial efecacy of topical agent extracted from Phellodendri cortex, Forsythia fructus and Glycyrrhizae radix as an alternative dermatological agent. Also, the most efficacious concentration of this agent and the skin-contact time inhibiting the vegetation of bacterial cells in various dilutions were determined. Five strains of Staphylococcus intermedius were exposed to various dilutions of this agent. The efficacy was assessed after exposure for time periods varying between 0 and 30 min. The antibacterial efficacy was shown to be the strongest at dilution of 1: 2. The number of viable organisms decreased significantly after 8 minutes of exposure time and was the smallest after 30 minutes of exposure time. This alternative dermatological agent is considered to be effective against Staphylococcus intermedius and has a potential to be a new topical agents far the treatment of pyoderma in dogs.

• Biomolecules & Therapeutics
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• 제3권3호
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• pp.229-232
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• 1995

In order to study if Glycyrrhizae Radix (GR) has protective effects on hepatotoxicity of acetaminophen in mouse, one of the species which are sensitive to acetaminophen-induced hepatotoxicity, effects of GR on liver weight to body weight ratio, serum alanine and aspartate transaminase (ALT and AST) activities, hepatic UDP-GT2 activity, and histopathologic changes were determined in acetaminophen-treated mice. Liver weight to body weight ratio and UDP-GT2 activity in mouse liver were not altered by GR. However, GR pretreatment lowered serum ALT and AST activities by 77% and 90% respectively, and diminished the degree of centrilobular necrosis caused by acetaminophen in liver as determined by histopathologic observation. These results suggest a possible protective effect of GR against the acetaminophen-induced hepatotoxicity in mice.

• 한방안이비인후피부과학회지
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• 제17권1호
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• pp.143-153
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• 2004

Antimicrobial activities against Streptococcus mutans, Candida albicans, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus were assayed through the hot water extracts and the ethanol extracts from Chungyulsodokeum and its composition oriental medicines. The stains were incubated on culture medium and activated. We observed the size of inhibition zone on the strains that is incubated in strilized filter paper disc with various concentration extracts of Chungyulsodokeum and its composition 1. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Glycyrrhizae Radix, Gardeniae Fructus showed antimicrobial activities against Staphylococcus aureus. 2. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Forsythiae Fructus Ghycyrrhizae Radix, showed antimiaobial activities against Staphylococcus epidermidis. 3. The extracts of Chungyulsodokeum, Coptidis Rhizoma, Paeoniae Radix, Glycyrrhizae Radix, showed antimicrobial activities against Streptococcus mutans. 4. The extract of Coptidis Rhizoma showed antimicrobial activities against Candida albicans. 5. None of the extracts showed antimicrobial activities against Escherichia coli. We observed antimicrobial activities of Chungyulsodokeum and its composition against Streptococcus mutans, Staphylococcus epidermidis, Staphylococcus aureus.

• 생약학회지
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• 제25권4호통권99호
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• pp.399-401
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• 1994

$Yongkaksan^{\circledR}$ is a powdered crude drug which is used to treat cough and recommended it as an expectorant. It is composed of Platycodi Radix (11.7 mg), Senegae Radix(0.5 mg), Armeniacae Semen(0.83 mg) and Glycyrrhizae Radix(8.3 mg). For the identification of individual ingredients in such powdery mixtures, microscopic method may be considered to be efficacious due to requirement of a small amount of specimens. In this paper, effectiveness of this method will be exemplified by the identification of the ingredients in $Yongkaksan^{\circledR}$ which contains 4 powdered crude drug ingredients.

• 대한본초학회지
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• 제25권3호
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• pp.123-130
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• 2010

Objectives & Method：We investigated adverse symptoms, toxicity, treatment and prevention against adverse effects of restoratives for invigorating qi(補氣藥) in order to use herbal medicines accurately. Result：Ginseng Radix(人參), Codonopsis Pilosulae Radix(黨參), Panacis Quinquefolii Radix(西洋參), Astragali Radix(黃芪), Atractylodis Rhizoma Alba(白朮), Dioscoreae Rhizoma(山藥), Dolichoris Semen(白扁豆), Glycyrrhizae Radix(甘草), Jujubae Fructus(大棗) and Mel(蜂蜜) may give rise to some side effects, allergic reaction or toxic symptoms in restoratives for invigorating qi(補氣藥). The representative methods of poisoning treatment in western medicines are stopping medication, washing out the stomach, promotion of vomiting, causing diarrhea, supplies of grape sugar and symptomatic treatment, etc. The representative methods of poisoning treatment in oriental medicine take advantage of various herbs. And Oriental medical doctor should meet symptoms as patients call for attention. In order to prevent against poisoning of restoratives for invigorating qi(補氣藥), the patients should keep usage, dosage and notes. Conclusion：We should pay attention to clinical using of Ginseng Radix(人參), Codonopsis Pilosulae Radix(黨參), Panacis Quinquefolii Radix(西洋參), Astragali Radix(黃芪), Atractylodis Rhizoma Alba(白朮), Dioscoreae Rhizoma (山藥), Dolichoris Semen(白扁豆), Glycyrrhizae Radix(甘草), Jujubae Fructus(大棗) and Mel(蜂蜜) in restoratives for invigorating qi(補氣藥).

• 분석과학
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• 제20권4호
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• pp.331-338
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• 2007

한약과 기능성식품으로 널리 활용되는 감초의 지표물질인 glycyrrhizin과 liquiritin을 HPLC-MS/MS 로 정성, 정량분석을 실시하였다. 두 가지 지표성분 모두 positive ion mode에서 검출이 가능하였고, precursor ion과 product ion을 조합한 multiple-reaction monitoring (MRM) mode에서 liquiritin은 m/z $436.2{\rightarrow}257.0$로 관찰되었고, glycyrrhizin은 m/z $823.4{\rightarrow}453.4$ 로 각각 검지되었다. 감초 물 추출물을 분무건조기로 건조한 분말에서 glycyrrhizin의 경우 5.8%, liquiritin의 경우 2.3% 각각 함유되어 있었다. 회수율은 103-113%였고, 일간, 일내 정밀도 실험에서 상대표준편차는 0.95-1.8% 였다. 본 실험 조건에서 liquiritin과 glycyrrhizin의 검출한계는 각각 0.4 ng/mL과 0.01 ng/mL 이었다.

• 한방안이비인후피부과학회지
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• 제13권1호
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• pp.209-236
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• 2000

This study has been carried out to investigate pathogenic factor and medicine of ja-shi(자시) by referring to literatures. The results were obtained as follows. 1. In oriental medicine, Chashi(차시), Hamshichang(함시창), Hamshi(함시), Jabal(자발), Shichang(시창), Nojaon(로자온), Hamaon(하마온), Jokshiong(적시옹), Hamshidok(함사독), Tabshijong(탑사종), Shijong(시종) were the other names of ja-shi(자시). 2. The pathogenic factors of ja-shi(자시) are wind-heat, wetness-heat, Kihyeolungche(기혈응체), stomach-heat. wetness, wind wetness, and liking greasy and sweet food. 3. In the internal therapy of ja-shi(자시), Bojesodokum(普濟消毒飮), Sasunchunglyangum(四順淸凉飮), Takljsodoksan(托裏消毒散), Shihogalguntang(柴胡葛根湯), Sungsohwan(醒消丸), Gamisodokum(加味消毒飮) and etc. were used. And Radix glycyrrhizae(甘草), Fructus forsythiae(連翹), Radix angelicae gogantis(當歸), Radix scutellariae(황기), Radix bupleuri(柴胡), Radix p]atycodi(桔梗), Radix Ledebounellae(防風), Frustus arctii(牛蒡子), Rhizoma cimicifugae(升麻) and ete. were used as medicine. 4. In the external therapy of ja-shi(자시), Youigumhwangsan(如意金黃散), Okiogo(玉露膏), Igumsan(二金散), Gumhwangsan(金黃膏), Nungo(嫩膏) and etc. were used. And Radix angelicae dahuricae(白芷), Cortex phellodendri(黃栢), Rhizoma rhei(大黃), Indigo maturalis(靑黛), Radix glycyrrhizae(甘草) and etc. were used as medicine.