• 한방안이비인후피부과학회지
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• 제27권3호
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• pp.96-105
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• 2014

Objective : Glycyrrhiza uralensis Fisch (GUF) has been used as remedy of allergic diseases for a long time in Korea. In the present study, we investigated the anti-allergic effects of GUF on experimental allergic asthma mouse model using ovalbumin (OVA). Methods : BALB/c mice were sensitized and challenged with 100 ug of OVA and 1 mg of aluminum potassium sulfate of 0.2 ml phosphate-buffered saline (PBS) intraperitoneally on day 1 and 15. Mice were challenged on 3 consecutive days with 5% OVA and AHR was assessed 24 hrs after the last challenge. We examined total inflammatory cell number in bronchoaveloar lavage fluid (BALF), Th2-associated cytokine productions and lung histology. Results : GUF potently inhibited the development of airway hypersensitivity and also reduced the number of BAL cells during OVA-induced allergic asthma. GUF also inhibited cytokine productions such as IL-4, IL-13 in lung tissue. Furthermore, GUF treatment inhibited allergic airway inflammation. Conclusion : These results suggest that GUF may inhibit the production of IL-4, IL-13 and infiltration of inflammatory cell and be beneficial oriental medicine for allergic asthma.

• 대한한방소아과학회지
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• 제32권3호
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• pp.16-25
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• 2018

Objectives The purpose of this study is to investigate the anti-inflammatory effect of Lonicera Japonica-Glycyrrhiza Uralensis decoction extracts (LGE) on ulcerative colitis in children and adolescents. Methods Colitis was induced by DSS (Dextran Sulfate Sodium) in C57BL/6 mice. The sample mice were divided into group of four. The mice in the control group were not inflammation-induced. The control group was composed of untreated ulcerative colitis elicited mice. The mice in the experimental group were administered with Pentasa and another experimental group mice were treated with LGE after colitis elicitation. The effects on ulcerative colitis were evaluated by the morphological changes of colonic mucosa, decrease in the effect of pro-inflammatory cytokines ($TNF-{\alpha}$ and $NF-{\kappa}B$) and inflammatory cytokines (iNOS and COX-2) in the mucosa. Results LGE showed protective effects in DSS induced ulcerative colitis. LGE inhibited shortening of colon length and relieved the hemorrhagic erosion in colonic mucosa. LGE decreased pro-inflammatory cytokines ($TNF-{\alpha}$ and $NF-{\kappa}B$) and inflammatory cytokines (iNOS and COX-2). According to the GC/MS analysis, N-methyl pyrrolidone (NMP) was identified. Conclusions The result shows the clinical efficacy of LGE and demonstrates possible treatment options for ulcerative colitis. Further investigations for biological activity and chemical analysis of LGE will be needed.

• 한국식품저장유통학회지
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• 제9권2호
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• pp.174-178
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• 2002

감초추출물의 농도를 100 ppm, 200 ppm, 300 ppm and 400 ppm로 조절하여 콩을 발아시켜 재배한 콩나물의 아미노산, 유기산 및 유리당 함량을 재배 2일, 3일 및 4일째에 분석하였다. 총 아미노산 함량은 100 ppm에서 3일간 재배하였을 때가 가장 높았고, 또한 재배일수가 길어질수록 aspartic acid 함량은 증가하지만, glutamic acid 함량은 오히려 감소하는 경향을 나타내었다. 총 유리당은 GGE 콩나물의 경우 대조군에 비하여 낮은 함량을 나타내었으며, 그러나 재배일수가 증가할수록 그 함량은 많아졌다 Sucrose함량은 재배일수가 길어질수록 감소하였으나, 다른 유리당은 오히려 증가하였다 총 유기산은 재배일수가 증가할수록 많은 함량을 나타내었으며, 200 ppm으로 재배한 콩나물이 가장 많은 함량을 나타내었다. 결론적으로 감초추출물은 콩나물의 품질증진을 위한 아미노산 및 유기산 함량을 증가시키는 발아 및 재배수로서 효과적일 것으로 판단된다.

• 한국약용작물학회지
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• 제18권1호
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• pp.28-33
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• 2010

Glycyrrhiza uralensis (Leguminosae) is a well-known herbal medicine that has long been valued as a demulcent to relieve inflammatory disorders. To compare the influence of different solvents on the anti-inflammatory efficacy of G. uralensis, we measured the inhibition of pro-inflammatory mediators such as NO, TNF-$\alpha$, and $PGE_2$ in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW 264.7 cells by extracts produced using different solvents (water, methanol, ethanol, or n-hexane). The results showed that methanol was the most effective solvent for the inhibition of both NO and $PGE_2$ production in RAW 264.7 cells. However, there was no difference among the extracts for inhibition of TNF-$\alpha$. Further study must be performed for the analysis of correlation between the anti-inflammatory activity of extracts produced using different solvents and the content of major bioactive compounds in G. uralensis, such as glycyrrhizin and liquiritin. The present study suggests that methanol may be a more appropriate solvent of G. uralensis than other solvents (water, ethanol, and n-hexane) to yield the greatest anti-inflammatory activity for food additives and medicine.

• 한국식품과학회지
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• 제30권6호
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• pp.1367-1372
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• 1998

한약재 혼합 효과와 chitosan 병용효과가 김치의 보존성에 미치는 영향을 조사하기 위하여 자초·감초 추출액 혼합 첨가(LG)와 절임 배추를 1% chitosan 용액에 침지 후 혼합 한약재를 첨가한 김치(LGDC)를 $10^{\circ}C$에서 25일간 발효시키면서 김치의 특성을 검토하였다. 김치 숙성 중 pH, 산도 총균수, 유산균수, 유산균 분포의 변화, 색택, 기호성을 대조구와 비교한 결과, LG군과 LGDC군은 대조구와 비교시, pH 감소속도는 완만하였고, 산도증가도 더 낮게 나타났으며, 총균수, 유산균수, Leuconostoc sp., L. plantarum 균수의 증식은 억제되었으며, LGDC군은 그 효과가 더욱 크게 나타났다. 특히 Leuconostoc sp., L. plantarum과 김치 유산균에 대해 초기부터 높은 억제능을 나타내었으며 이로써 chitosan에 대한 유산균 특이성에 대한 연구가 더욱 진행되어야 할 것으로 사료되었다. 김치의 관능검사 결과 신맛은 대조구에 비해 낮게 나타났으며 향, 종합적 기호도 등은 대조구와 뚜렷한 차이를 보이지 않아 자초, 감초 및 chitosan은 김치 숙성을 지연시키기 위한 첨가제로서 적당한 것으로 나타났다.

• 한방안이비인후피부과학회지
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• 제17권2호
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• pp.12-30
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• 2004

This study was carried out to investigate the effects of SMG on the in vitro and in vivo inflammatory reactions. In experiment I, in vitro tests, ethanol extract of SMG showed potent radical scavenging activity tested by DPPH(I,1-diphenyl-2-picryl-hyrazyl) method and inhibited the lipopolysaccharide-induced gene expressions of interleukin-1${\beta}$, interleukin-6 and tumor necrosis factor-${\alpha}$ (above 50$\%$ at a concentration of 50㎍／㎖) by the macrophage RAW 246.7 cells. Among the herbal ingredients of SMG, ethanol extracts of Scutellaria baicalensis, Paeonia lactiflora, Glycyrrhiza glabra showed potent radical scavenging activity. And Glycyrrhiza glabra and Scutellaria baicalensis showed potent inhibitory activity of nitric oxide production. Especially, ethanol extract of Scutellaria baicalensis inhibited the gene expression of IL-1${\beta}$, IL-6 and TNF-${\alpha}$. In cyclooxygenase-2 assay, Scutellaria baicalensis and Glycyrrhiza glabra showed the potent inhibition of prostaglandin E2 generation. In experiment 2, in vivo tests, SMG showed inhibitory effects on vascular permeability (28.7$\%$) and leukocyte migration (11.5$\%$). These results mean that SMG has a anti-inflammatory effects by it's inhibitory effects of leukocyte migration and vascular permeability as well as it's inhibitory effects of lipopolysaccharide-induced gene expression of IL-1${\beta}$, IL-6 and TNF-${\alpha}$, and radical scavenging activity. Therefore, I expect that SMG may be used as a effective drug for treatment on inflammation.

• 한국약용작물학회지
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• 제25권5호
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• pp.315-321
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• 2017

Background: Glycyrrhiza uralensis Radix (GR) is a crude drugs used in Asian countries that has been reported to prevent the progression of neurodegenerative diseases such as Alzheimer's disease. The present study examined whether GR and its active compounds, glycyrrhizic acid (GA) and isoliquiritigenin (IL), exerted protective effects on $H_2O_2$-induced oxidative damage in C6 glial cells. Methods and Results: We exposed C6 glial cells to hydrogen peroxide ($H_2O_2$) for 24 h and investigated the cellular response to GR and its active compounds by evaluating cell viability, reactivie oxygen species (ROS) production, and apoptosis-related protein expression. GR successfully mitigated the reduced cell viability and ROS production induced by $H_2O_2$ in C6 glial cells, IL and GA significantly increased the cell viability and decreased ROS production. In addition, IL and GA down-regulated apoptotic Baxdependent caspase-3 activation, but each compound exerted different mechanisms, i.e., IL dose-dependently decreased ROS production and, GA up-regulated anti-apoptotic Bcl-2 expression. Conclusions: These results demonstrated that GR and its active components, IL and GA, exhibit potential for use as natural neurodegenerative agents for the modulation of apoptosis in C6 glial cells.

• 한국식품과학회지
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• 제38권1호
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• pp.143-146
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• 2006

본 연구의 목적은 김치의 맛을 증진시키기 위하여 감미료로 첨가되는 설탕 대신에 분말상태의 감초를 첨가하여 김치의 품질특성을 개선하고자 하는 것이다. 김치 제조원료에 설탕 또는 감초를 첨가하여 젖산균의 생육과 산생성 및 관능성 특성에 미치는 영향을 조사하였다. 김치에 첨가된 감초의 영향은 다음과 같다. pH는 비숙성시료와 1일 숙성시료는 감초의 첨가에 따라 차이가 없었으나, 2일 숙성시료와 3일 숙성시료의 경우는 감초의 첨가에 따라 pH가 다소 높아지는 경향을 보였다. 산도는 비숙성시료와 1일 숙성시료는 감초의 첨가농도가 증가함에 따라 유의적으로 증가하였으나(p<0.05), 2일 숙성시료와 3일 숙성시료의 경우는 반대로 감초의 첨가농도가 증가함에 따라 유의적으로 감소하였다(p<0.05). 감초의 첨가에 의하여 젖산균수는 변화가 없었다. 관능검사의 결과를 보면 1-3일 숙성된 시료의 전반적인 기호도와 맛은 감초 0.1% 또는 0.2% 첨가시료가 표준시료와 같거나 다소 우수하였고, 1.0% 첨가시료가 가장 저조하였다. 냄새와 조직감은 1.0% 첨가시료가 다른 시료보다 저조하였고, 색상은 시료 사이에 차이가 없었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.88-88
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• 2019

Antioxidants are involved in the defense mechanism against the attack of free radicals. This study was carried out to determine the antioxidant activity of new variety of Glycyrrhiza cultivar radix, Wongam and Sinwongam. Dissolved freeze dried Wongam and Sinwongam extracts were filtered by $0.2{\mu}m$ filter and serially diluted at the concentrations of $10{\mu}g/mL$, $50{\mu}g/mL$, $100{\mu}g/mL$, $500{\mu}g/mL$, and $1000{\mu}g/mL$. The antioxidant potential was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, ABTS (2,2-azino-bis (3-rthylbenzthiazoline-6-sulfonic acid) diammonium salt) radical cation decolorization assay, nitrite radical scavenging assay, and ferric reducing antioxidant power (FRAP) assay. DPPH radical scavenging activities (i.e. the highest value $50.9{\pm}0.8%$ by Wongam and $82.6{\pm}1.1%$ by Sinwongam), ABTS radical scavenging activities (i.e. the highest value $88.1{\pm}1.8%$ by Wongam and $98.6{\pm}0.1%$ by Sinwongam), and nitrite radical scavenging activities (i.e. the highest value $87.3{\pm}1.6%$ by Wongam and $89.8{\pm}0.8%$ by Sinwongam) increased in a dose-dependent manner. In addition, ferric reducing power activities also increased in a dose-dependent manner. The FRAP value of Wongam and Sinwongam extracts were $0.72{\pm}0.03$ and $0.99{\pm}0.06$ compared to ascorbic acid, as a positive control, was $1.32{\pm}0.02$. These results suggested that Wongam and Sinwongam have beneficial effects as a potent antioxidant.

• 동의생리병리학회지
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• 제33권2호
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• pp.116-122
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• 2019

The aim of this study is to evaluate the anti-inflammatory effect of Hataedock treatment using Coptidis Rhizome and Glycyrrhiza Uralensis (CG) mixed extract in allergic rhinitis induced NC/Nga mice. We divided NC/Nga mice into 3 groups as follows; allergic rhinitis-induced group after CG Hataedock treatment (CGT, n=10), no treatment group (Ctrl), allergic rhinitis elicited group (ARE). To induce allergic rhinitis, NC/Nga mice of 3 weeks age were sensitized on 7, 8 and 9week by Ovalbumin (OVA) antigen in intranasal space. Hataedock using CG extract was administered on week 3 in allergic rhinitis-induced group (CGT) after Hataedock treatment. To identify distribution of Interlukin (IL)-4, Cluster of differentiation 40 (CD40), high-affinity IgE receptor ($Fc{\varepsilon}RI$), substance P, Matrix metallopeptidase 9 (MMP-9), Nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) p65, Inducible nitric oxide synthase (iNOS) and Cycloxygenase-2 (COX-2), we used histological examination. CGT significantly inhibited IL-4 and CD40 response compared with ARE. The reduction of Th2 cytokine expression decreased inflammatory mediators such as $Fc{\varepsilon}RI$, substance P, MMP-9, $NF-{\kappa}B$ p65, iNOS and COX-2. Such immunological improvement induced reduction of respiratory epithelial damage and mucin secretion in goblet cell. These results indicate that Hataedock treatment suppresses allergic rhinitis through modulating of Th2 responses and diminishing various inflammatory mediators in nasal mucosal tissue. It might have potential applications for prevention and treatment of allergic rhinitis.