Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Protective Effects of Glycyrrhiza uralensis Radix Extract and Its Active Compounds on H2O2-induced Apoptosis of C6 Glial Cells

H2O2로 유도된 C6 신경교세포의 세포사멸에 대한 감초 추출물과 감초 활성물질의 보호효과

  • Park, Chan Hum (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Ji Hyun (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Seung Hak (Department of Food Science and Nutrition, Pusan National University) ;
  • Shin, Yu Su (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Sang Won (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
  • 박찬흠 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김지현 (부산대학교 식품영양학과) ;
  • 최승학 (부산대학교 식품영양학과) ;
  • 신유수 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이상원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 조은주 (부산대학교 식품영양학과)
  • Received : 20170631
  • Accepted : 2017.09.07
  • Published : 2017.10.30
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Abstract

Background: Glycyrrhiza uralensis Radix (GR) is a crude drugs used in Asian countries that has been reported to prevent the progression of neurodegenerative diseases such as Alzheimer's disease. The present study examined whether GR and its active compounds, glycyrrhizic acid (GA) and isoliquiritigenin (IL), exerted protective effects on $H_2O_2$-induced oxidative damage in C6 glial cells. Methods and Results: We exposed C6 glial cells to hydrogen peroxide ($H_2O_2$) for 24 h and investigated the cellular response to GR and its active compounds by evaluating cell viability, reactivie oxygen species (ROS) production, and apoptosis-related protein expression. GR successfully mitigated the reduced cell viability and ROS production induced by $H_2O_2$ in C6 glial cells, IL and GA significantly increased the cell viability and decreased ROS production. In addition, IL and GA down-regulated apoptotic Baxdependent caspase-3 activation, but each compound exerted different mechanisms, i.e., IL dose-dependently decreased ROS production and, GA up-regulated anti-apoptotic Bcl-2 expression. Conclusions: These results demonstrated that GR and its active components, IL and GA, exhibit potential for use as natural neurodegenerative agents for the modulation of apoptosis in C6 glial cells.

Keywords

References

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