• Journal of Microbiology and Biotechnology
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• v.33 no.12
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• pp.1692-1697
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• 2023

Staphylococcus aureus integrated with mecA gene, which codes for penicillin-binding protein 2a, is resistant to all penicillins and other beta-lactam antibiotics, resulting in poor treatment expectations in skin and soft tissue infections. The development of a simple, sensitive and portable biosensor for mecA gene analysis in S. aureus is urgently needed. Herein, we propose a dual-toehold-probe (sensing probe)-mediated exonuclease-III (Exo-III)-assisted signal recycling for portable detection of the mecA gene in S. aureus. When the target mecA gene is present, it hybridizes with the sensing probe, initiating Exo III-assisted dual signal recycles, which in turn release numerous "3" sequences. The released "3" sequences initiate catalytic hairpin amplification, resulting in the fixation of a sucrase-labeled H2 probe on the surface of magnetic beads (MBs). After magnet-based enrichment of an MB-H1-H2-sucrase complex and removal of a liquid supernatant containing free sucrase, the complex is then used to catalyze sucrose to glucose, which can be quantitatively detected by a personal glucose meter. With a limit of detection of 4.36 fM for mecA gene, the developed strategy exhibits high sensitivity. In addition, good selectivity and anti-interference capability were also attained with this method, making it promising for antibiotic tolerance analysis at the point-of-care.

• Journal of Sensor Science and Technology
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• v.22 no.3
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• pp.202-206
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• 2013

In this paper, a disposable glucose sensor was made of paper. Glucose sensor strip using carbon electrode is appropriate for the low price ones because it requires cheap materials and low cost production. Most of blood glucose sensors were developed with plastics, but it causes pollution problems. Therefore we developed disposable carbon electrode glucose sensor using paper. This sensor consists of upper and bottom plate. On the upper plate, three-dimensional channel are formed through pressing process. The fabricated paper glucose sensor shows relatively short sensing time of about 5seconds, excellent reproducibility ($R^2$=0.9558), and fabrication yield as well.

• Journal of Biosystems Engineering
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• v.28 no.5
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• pp.465-468
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• 2003

A microdialysis coupled flow injection amperometric biosensor was calibrated to measure the concentration of glucose using 7 standard samples from 10ml to 70ml of glucose solution. The output of the sensor increased linearly with an increase in the glucose concentration with an $R^2$ correlation of 0.99. The amperometric biosensor was then applied to measure the. glucose concentration of 2 commercial samples(Orange and Pineapple juice) and the results compared with HPLC. Around 12% error was observed in glucose concentration measurements of the samples analyzed. The sensor has potential in rapid measurement once the calibration is done. Potential for on-line sensing is also discussed.

• Journal of Biomedical Engineering Research
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• v.33 no.3
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• pp.114-127
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• 2012

Recent technical advancement allows noninvasive measurement of blood glucose. In this literature, we reviewed various noninvasive techniques for measuring glucose concentration. Optical or electrical methods have been investigated. Optical techniques include near-infrared spectroscopy, Raman spectroscopy, optical coherence technique, polarization, fluorescence, occlusion spectroscopy, and photoacoustic spectroscopy. Electrical methods include reverse iontophoresis, impedance spectroscopy, and electromagnetic sensing. Ultrasound, detection from breath, or fluid harvesting technique can be used to measure blood glucose level. Combination of various methods is also promising. Although there are many interesting and promising technologies and devices, there need further researches until a commercially available non-invasive glucometer is popular.

• The Korea Genome Conference
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• 2003.09a
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• pp.40.1-40.1
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• 2003

• Journal of Sensor Science and Technology
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• v.28 no.1
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• pp.47-51
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• 2019

Sweat-based glucose sensors are being widely investigated and researched as they facilitate painless and continuous measurement. However, because the concentration of sweat glucose is almost a hundred times lower than that of blood glucose, it is important to develop electrochemical sensing electrode materials that are highly sensitive to glucose molecules for the detection of low concentrations of glucose. The preparation of a flexible and ultra-sensitive sensor for detection of sweat glucose is presented in this study. Oxygen and nitrogen are removed from the surface of a polyimide film by exposure to a CO2 laser; hence, laser-induced graphene (LIG) is formed. The fabricated LIG electrode showed favorable properties of high roughness and good stability, flexibility, and conductivity. After the laser scanning, Pt nanoparticles (PtNP) with good catalytic behavior were electrodeposited and the glucose sensor thus developed, with a LIG/PtNP hybrid electrode, exhibited a high order of sensitivity and detection limit for sweat glucose.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.07a
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• pp.451-454
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• 2001

In this study, a micro-glucose sensor was fabricated by micromachining technology and its sensing characteristics were investigated. The 7740 pyrex glass was used as the bottom substrate and anisotropically etched silicon wafer was used as the top substrate. The size of the fabricated microchip is 1.58${\times}$1.58mm$^2$. It is shown that output current exhibits a linear change according to glucose concentration (100 mM ∼ 300 mM). It is also shown that the response time for glucose was within 240 sec. It was followed by a saturation trend within 50 sec. The g1ucose sensor with Fc$\^$+/ exhibits relatively higher sensitivity than that without Fc$\sub$+/ for output current.

• Journal of Sensor Science and Technology
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• v.23 no.5
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• pp.295-298
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• 2014

A novel approach to fabricating high-performance glucose sensors is reported, which is based on the process of self-assembled monolayers (SAMs). In this study, we have particularly used ${\alpha}$-lipoic acid (LA) SAMs for the glucose sensors. To our best knowledge, this study is the first one to use LA as SAMs for this purpose. N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were deliberately attached at the same time on the LA SAM. Then, glucose oxidase ($GO_X$) and horseradish peroxidase (HRP) were sequentially immobilized. Thus, the HRP/$GO_X$/NHS-EDC/LA-SAM/Au/Cr/glass working electrode was developed. The glucose-sensing capability of the fabricated sensor was systematically measured by the use of cyclic voltammetry in the range of 1-30 mM glucose in phosphate-buffered saline. The result showed a good sensitivity, that is, as high as $27.5{\mu}A/(mM{\cdot}cm^2)$. This result conspicuously demonstrates that LA can be one of promising substances for use as SAMs for accurately monitoring trace levels of glucose concentration in human blood.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.42 no.2 s.302
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• pp.33-38
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• 2005

In this paper, the sensing unit of a non-invasive blood glucose sensor for home users, using a magneto-resonance absorption method, have been designed and manufactured. The sensor is capable of non-invasively determining blood glucose levels through measuring the 1H spin-lattice relaxation time in human body, The comparison of initial models, with different dimensions and shapes, for the sensing unit has led us to select the materials of the final model, which has adequate size and weight for home use. Through the design optimization using the FEM model, the dimension of final model has been determined to satisfy the required strength and uniformity of the magnetic field in the detecting area.

• Applied Chemistry for Engineering
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• v.27 no.2
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• pp.145-152
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• 2016

In this study, CuO was introduced on MWCNTs dispersed with Au nanoparticles to improve the glucose sensing capability of electrochemical biosensors. Nano-cluster shaped CuO was synthesized due to the presence of Au nanoparticle, which affects glucose sensing performance. The biosensor featuring CuO/Au@MWCNTs nanocomposite as an electrode material when 0.1 mole of CuO was synthesized showed the highest sensitivity of $504.1{\mu}A\;mM^{-1}cm^{-2}$, which is 4 times better than that of MWCNTs based biosensors. In addition, it shows a wider linear range from 0 to 10 mM and lower limit of detection (LOD) of 0.008 mM. These results demonstrate that CuO/Au@MWCNTs nanocomposite sensors are superior to other CuO based biosensors which are attributed that the nano-cluster shaped CuO is favorable for the electrochemical reaction with glucose molecules.