• Analytical Science and Technology
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• v.23 no.2
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• pp.165-171
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• 2010

Glucose oxidase ($GOD_{ox}$) immobilized biosensor was fabricated by two methods. In one of the methods, gold nanoparticles (Au-NPs) prepared by ${\gamma}$-irradiation were loaded into the poly(maleic anhydride)-grafted multi-walled carbon nanotube, PMAn-g-MWCNT electrode via physical entrapment. In the other method, the Au-NPs were prepared by electrochemical reduction of Au ions on the surface of PMAn-g-MWCNT electrode and then GODox was immobilized into the Au-NPs. The $GOD_{ox}$ immobilized biosensors were tested for electrocatalytic activities to sense glucose. The sensing range of the biosensor based on the Au-NPs physically modified PMAn-g-MWCNT electrode was from $30\;{\mu}M$ to $100\;{\mu}M$ for the glucose concentration, and the detection limit was $15\;{\mu}M$. Interferences of ascorbic acid and uric acid were below 7.6%. The physically Au deposited PMAn-g-MWCNT paste electrodes appear to be good sensor in detecting glucose.

• Microbiology and Biotechnology Letters
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• v.20 no.4
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• pp.395-400
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• 1992

A novel strain of HSL613 producing a large amount of cholesterol oxidase as an extra~ cellular enzyme was isolated from soil samples. Experiments were carried out to optimize the condition of cholesterol oxidase production using HSL613 strain. This microorganism was shown to give the maximum yield f)f cholesterol oxidase in the medium containing 2% glucose, 2% yeast extract, 0.2% $K_2HP0_4$, 0.1% NaCl. 0.005% $CaCl_22H_2O, 0.001% $FeSO_47H_20$. The optimum temperature was $30^{\circ}C$ and the enzyme production reached a maximum level at 144 hours of cultivation (10.3$\mu$/ml).

• Journal of the Korean Applied Science and Technology
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• v.36 no.1
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• pp.269-278
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• 2019

We fabricated glucose oxidase (GOx)-modified biosensor for detection of glucose by physical immobilization of GOx after electrochemical polymerization of the conductive mixture monomers of the 3-thiophenecarboxylic acid (TCA) and thiophene (Th) onto ITO electrode in this study. We confirmed the successfully fabrication of GOx-modified biosensor via FT-IR spectroscopy, SEM, contact angle, and cyclic voltammetry. The fabricated biosensor has the detection limit of $0.1{\mu}M$, the linearity of 0.001-27 mM, and sensitivity of $38.75mAM^{-1}cm^{-2}$, respectively. The fabricated biosensor exhibits high interference effects to dopamine, ascorbic acid, and L-cysteine, respectively. From these results, the fabricated GOx-modified biosensor with long linearity and high sensitivity could be used as glucose sensor in human blood sample.

• Journal of Sensor Science and Technology
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• v.32 no.1
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• pp.16-21
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• 2023

This study reports the potential of using commercial copy papers as substrates for simple sensitive glucose detection. Typical paper-based devices use filter papers as porous substrates that can contain reagents; however, this is the first study to report the use of copy papers for the purpose of enhancing enzymatic colorimetric detection. Glucose detection using glucose oxidase, horseradish peroxidase and potassium iodide was performed on a copy paper, cellulose-based filter paper, and polyethylene film. The results indicated that the copy paper exhibited a stronger coloration than the other substrates. Reagents required for detection were dried on the copy paper, and a 3D-printed holder was designed to provide an environment for consistent imaging, making it a convenient cost-effective option for point-of-care testing using a mobile phone camera. The simple paper-based glucose sensor exhibited a linear range of 0.1-20 mM, limit of quantification of 0.477 mM, and limit of detection of 0.143 mM.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.1
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• pp.141-145
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• 2002

In order to clarify the neuroprotective effect of Gamibojungikki-tang (GBJIKT) water extract on cultured mouse spinal sensory neuron damaged by glucose Oxidase (GO), MTT [3-(4,5-dimethylthiazole-2-yl) -2,5-diphenyltetrazolium bromide] assay and SRB (Sulforhodamine B) assay were carried out after the cultured mouse spinal sensory neuron were preincubated with various concentrations of GBJIKT water extract for 3 hours prior to exposure of GO. Cell viability of cultured mouse spinal sensory neurons exposed to various concentrations of GO for 8 hours was decreased in a dose-dependent manner. MTT50 values were 45 mU/ml GO. Cultured mouse spinal sensory neurons in the medium containing various concentration of GO for 8 hours showed decreasing of total protein synthesis. GO was toxic on cultured spinal sensory neurons. Pretreatment at GBJIKT water extract for 3 hours following GO prevented the GO-induced neurotoxicity such as decreasing of total protein synthesis. These results suggest that GO shows toxic effect on cultured spinal sensory neurons and GBJIKT water extract is highly effective in proecting the neurotoxicity induced by GO.

• Journal of Oriental Neuropsychiatry
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• v.10 no.1
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• pp.53-78
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• 1999

As the average life span have been lengthened and the rate of senile population have been raised, chronic degenerative diseases incident to aging has been increased rapidly and become a social problem. With this social background, recently, the facts that oxygen radicals(OR) have toxic effects on Central Nervous System and Peripheral Nervous System and cause neuropathy such as Parkinson's Disease, Alzheimer Disease have been turned out, and accordingly lots of studies on the mechanism of the toxic effects of OR on nerves, the diseases caused by OR and the approaches to curing the diseases have been made. The purpose of this study is to examine the toxic effects caused by Glucose Oxidase(GO) and the effects of herbal extracts such as Chilbokyeum(CBY), Chilbokyeumga Acori Rhizoma(CAR), Acori Rhizoma(AR) on the treatment of the toxic effects. For this purpose, experiments with the cultured cell from the cerebrums of new born mice were done. The results of these experiments were as follows. 1. GO, a oxygen radical, decreased the survival rate of the cultured cells on NR assay, MTT assay and amount of neurofilaments and increased the amount of total protein, lipid peroxidation and the amount of LDH. 2. CBY have efficacy of increasing the amount of neurofilaments and total protein and decreasing lipid peroxidation and the amount of LDH. 3. CAR have efficacy of increasing the amount of neurofilaments and total protein and decreasing lipid peroxidation and the amount of LDH. 4. AR have efficacy of increasing the amount of neurofilaments and total protein. From the above results, It is concluded that Chilbokyeumgamibang has marked efficacy as a treatment for the damages caused in the GO-mediated oxidative process. And Chilbokyeumgamibang is thought to have certain pharmacological effects on controlling over aging and treating Dementia. Further clinical study of this pharmacological effects of Chilbokyeumgamibang should be complemented.

• The Korean Journal of Mycology
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• v.22 no.4
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• pp.325-331
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• 1994

White-rot fungus, Schizopora paradoxa did not produce Mn-peroxidase and glucose oxidase without manganese. But, in high concentration of manganese (40 ppm), the activities of both enzymes were higher than those in basal concentration of manganese (11.15 ppm). Unlike the activities of the enzymes, mycelial mass was the same level as the control culture (11.15 ppm manganese) through out the culture period, depending on the concentration of manganese. The same experiments were carried out for the effect of copper and veratryl alcohol added to the culture. The results were not consistent dependent on the concentration of copper and veratryl alcohol, respectively. The involvement of cAMP in the correlative production of MNP and GOX was investigated. In this study, addition of atropine to the culture resulted in a concomitant inhibition of production of MNP and GOX, depending on the concentration of inhibitor added.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.3
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• pp.495-498
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• 2002

Cytotoxicity of glucose oxidase(GO) and neuroprotective effect of Aconiti Radix(AKR) against GO-induced neurotoxicity were measured for elucidating the mechanism of cardiotoxicity on cultured mouse myocardial cells by MTT assay after myocardial cells were cultured for 24 hours at various concentrations of GO. GO was toxic in a time-and dose-dependent manner on cultured myocardial cells after myocardial cells were grown for 24 hours in media containing 5～40mU/ml GO. While, cultures were pretreated with 50 μg/ml AKR for 2 hours increased remarkably cell viability. From the above results, it is suggested that GO is toxic on cultured mouse myocardial cells by the decrease of cell viability, and herb medicine such as AKR is very effective in the prevention of myocardial toxicity induced by GO.

• Herbal Formula Science
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• v.7 no.1
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• pp.143-151
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• 1999

To evaluate the neurotoxic effect of oxygen radicals in cultured mouse spinal dorsal root ganglion(DRG) neurons, cytotoxicity was determined by MTT assay after cultured DRG neurons were grown in the media containing various concentrations of glucose oxidase(GO). In addition, neuroprotective effect of herb extract, Epimedium Koreanum Nakai was examined by MTT assay in cultured DRG neurons. Cell viability of cultured DRG neurons was remarkably decreased by GO in dose- and time-dependent manner, and Epimedium Koreanum Nakai protected remarkably GO-induced neurotoxicity in these cultures. From the above results, it is suggested that oxygen radicals is toxic in cultured mouse DRG neurons, and herb extracts such as Epimedium Koreanum Nakai are effective in prevention of the neurotoxicity induced by oxygen radicals in cultured mouse DRG neurons.

• Journal of the Korean Chemical Society
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• v.37 no.10
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• pp.874-880
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• 1993

Spectrophotometric enzymatic analysis and amperometric enzymatic analysis for the determinations of glucose and ethanol were studied utilizing glucose oxidase (GO) and alcohol oxidase (AO), respectively, which commonly consume $O_2$ and produce $H_2O_2$. For the determination of glucose, $H_2O_2$ were coupled to $K_4Fe(CN)_6$ via peroxidase producing $K_3Fe(CN)_6$ whose absorbance was measured at 418 nm or whose diffusion current was measured on the glassy carbon electrode at an applied potential of -55 mV vs. Ag/AgCl (sat. KCl) reference electrode. Amperometric analysis was 1000 times more sensitive as well as 10 times better in the linear concentration range than spectrophotometric analysis. For the determination of ethanol, AO only was used for the enzymatic analysis, since $K_3Fe(CN)_6$ was completely disappeared as soon as AO was added. Either rate of $H_2O_2$ produced was amperometrically measured at +0.900 V or rate of $O_2$ consumed was measured at -0.500 V vs. Ag/AgCl(sat. KCl) reference electrode.